BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--1575 (768 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g18180.1 68417.m02701 glycoside hydrolase family 28 protein /... 29 4.5 At1g56590.1 68414.m06508 clathrin adaptor complexes medium subun... 29 4.5 At5g36090.1 68418.m04349 hypothetical protein contains Pfam prof... 28 7.9 At1g42250.1 68414.m04869 replication protein-related weak simila... 28 7.9 At1g23860.2 68414.m03010 splicing factor RSZp21 (RSZP21) / 9G8-l... 28 7.9 At1g23860.1 68414.m03009 splicing factor RSZp21 (RSZP21) / 9G8-l... 28 7.9 >At4g18180.1 68417.m02701 glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein similar to polygalacturonase [Salix gilgiana] GI:6714524; contains PF00295: Glycosyl hydrolases family 28 (polygalacturonases) Length = 414 Score = 28.7 bits (61), Expect = 4.5 Identities = 18/75 (24%), Positives = 33/75 (44%) Frame = -1 Query: 432 KNVA*KYSKLFLIEKHTPRSTP*PKYNIVILDYDLQNNTTQH*PERSFTREN*TITAFLR 253 KN+ S L ++ H R P K + + DL ++ ++S R N +++ R Sbjct: 341 KNIRGTSSSLVAVQLHCSRGMPCKKVYLENVHLDLSSSDGGR--KQSSNRGNEAVSSSCR 398 Query: 252 LLGSEYLGVSFTDPC 208 + + Y+G PC Sbjct: 399 NVRANYIGTQIPPPC 413 >At1g56590.1 68414.m06508 clathrin adaptor complexes medium subunit family protein contains Pfam profile: PF00928 adaptor complexes medium subunit family Length = 415 Score = 28.7 bits (61), Expect = 4.5 Identities = 16/53 (30%), Positives = 26/53 (49%), Gaps = 2/53 (3%) Frame = -2 Query: 692 SGNQAIITLLQKKNCGWTYWTIRKHSLGHIS--IALKM*TQRIRIT*SHVLGY 540 S N +T+L K C WT I K +S +AL+ +R+ + + LG+ Sbjct: 322 SSNHGTVTILSNKTCTWTIGRIPKDKTPCLSGTLALEPGLERLHVFPTFKLGF 374 >At5g36090.1 68418.m04349 hypothetical protein contains Pfam profile PF03778: Protein of unknown function (DUF321) Length = 141 Score = 27.9 bits (59), Expect = 7.9 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = -1 Query: 711 ELFGLKVW*PSHYYTFAKKKLRLDILDNK 625 ++ GL+ W +H +TF +KLR L K Sbjct: 53 KITGLRFWRENHGFTFLARKLRFTFLTGK 81 >At1g42250.1 68414.m04869 replication protein-related weak similarity to Replication Protein A 70 kDa DNA-binding subunit (RP-A) (RF-A) (Replication factor-A protein 1) (Single-stranded DNA-binding protein) (DMRPA1) (GI:2498844)(SP:Q24492) [Drosophila melanogaster] Length = 114 Score = 27.9 bits (59), Expect = 7.9 Identities = 9/22 (40%), Positives = 16/22 (72%) Frame = -1 Query: 591 QNVNPENSYHLISRIRLHTIQH 526 ++V+P++ YH I + HTI+H Sbjct: 2 EHVSPKSRYHFIECVDFHTIKH 23 >At1g23860.2 68414.m03010 splicing factor RSZp21 (RSZP21) / 9G8-like SR protein (SRZ21) nearly identical to 9G8-like splicing factor SRZ21 [Arabidopsis thaliana] GI:3435096, RSZp21 protein [Arabidopsis thaliana] GI:2582643 Length = 187 Score = 27.9 bits (59), Expect = 7.9 Identities = 19/58 (32%), Positives = 26/58 (44%) Frame = +2 Query: 62 RDTSPAVTRSKRYTGADRRKTARAC*RPPKRPSSAKTRVQTKLTRPRCSQGSVKDTPK 235 R SP RS Y A R + R PP+R S R +R +GS +D+P+ Sbjct: 114 RSPSPRRRRSPDYGYARRSISPRGRRSPPRRRSVTPPRRGRSYSRSPPYRGSRRDSPR 171 >At1g23860.1 68414.m03009 splicing factor RSZp21 (RSZP21) / 9G8-like SR protein (SRZ21) nearly identical to 9G8-like splicing factor SRZ21 [Arabidopsis thaliana] GI:3435096, RSZp21 protein [Arabidopsis thaliana] GI:2582643 Length = 187 Score = 27.9 bits (59), Expect = 7.9 Identities = 19/58 (32%), Positives = 26/58 (44%) Frame = +2 Query: 62 RDTSPAVTRSKRYTGADRRKTARAC*RPPKRPSSAKTRVQTKLTRPRCSQGSVKDTPK 235 R SP RS Y A R + R PP+R S R +R +GS +D+P+ Sbjct: 114 RSPSPRRRRSPDYGYARRSISPRGRRSPPRRRSVTPPRRGRSYSRSPPYRGSRRDSPR 171 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,131,851 Number of Sequences: 28952 Number of extensions: 327722 Number of successful extensions: 672 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 653 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 672 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1721869952 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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