Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1569
(659 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC736.13 |||short chain dehydrogenase|Schizosaccharomyces pomb... 54 2e-08
SPAC521.03 |||short chain dehydrogenase |Schizosaccharomyces pom... 38 0.001
SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces pomb... 38 0.001
SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr ... 35 0.009
SPAC22A12.17c |||short chain dehydrogenase |Schizosaccharomyces ... 34 0.021
SPCC162.03 |||short chain dehydrogenase |Schizosaccharomyces pom... 33 0.036
SPAC4G9.15 |||ketoreductase |Schizosaccharomyces pombe|chr 1|||M... 32 0.084
SPAC4H3.08 |||short chain dehydrogenase |Schizosaccharomyces pom... 31 0.15
SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase Ubp4|Schizos... 31 0.15
SPCC1739.08c |||short chain dehydrogenase|Schizosaccharomyces po... 31 0.15
SPAC19A8.06 |||short chain dehydrogenase|Schizosaccharomyces pom... 30 0.26
SPBC30D10.05c |||sepiapterin reductase |Schizosaccharomyces pomb... 30 0.34
SPCC1450.15 |||pig-F |Schizosaccharomyces pombe|chr 3|||Manual 29 0.59
SPAC513.07 |||flavonol reductase/cinnamoyl-CoA reductase family|... 29 0.78
SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces ... 28 1.4
SPBC1703.05 |||protein kinase, RIO family|Schizosaccharomyces po... 27 1.8
SPBC342.01c |alg6|SPBC3F6.06c|glucosyltransferase Alg6|Schizosac... 27 2.4
SPAC977.08 |||short chain dehydrogenase |Schizosaccharomyces pom... 27 2.4
SPBC1348.09 |||short chain dehydrogenase |Schizosaccharomyces po... 27 2.4
SPBC16E9.14c |||membrane transporter|Schizosaccharomyces pombe|c... 27 3.2
SPBC17A3.07 |pgr1||glutathione reductase|Schizosaccharomyces pom... 26 4.2
SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces p... 26 5.5
SPAC29A4.15 |||serine-tRNA ligase|Schizosaccharomyces pombe|chr ... 26 5.5
SPCC63.08c |ppk36|atg1|serine/threonine protein kinase Ppk36|Sch... 25 7.3
SPAC6F12.02 |rst2||transcription factor Rst2|Schizosaccharomyces... 25 7.3
SPAC24C9.14 |otu1|mug141|ubiquitin-specific protease |Schizosacc... 25 7.3
SPBC1709.07 |erg27||3-keto sterol reductase |Schizosaccharomyces... 25 9.7
SPAC4H3.02c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 9.7
>SPCC736.13 |||short chain dehydrogenase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 339
Score = 53.6 bits (123), Expect = 2e-08
Identities = 25/70 (35%), Positives = 44/70 (62%)
Frame = +2
Query: 62 DLKGKVALITGAASGIGLAYSEELLKQGAKVSLCDIDSERGEQVTEKLASKYGRKNVLFC 241
DL GKVAL+TG++ GIG + EL ++GAKV L + E+ ++V +++ + + F
Sbjct: 39 DLTGKVALVTGSSGGIGYVTALELARKGAKVYLAGRNEEKYQKVMKQIHDEVRHSKIRFL 98
Query: 242 RLDVMTIHNL 271
RLD++ ++
Sbjct: 99 RLDLLDFESV 108
>SPAC521.03 |||short chain dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 259
Score = 38.3 bits (85), Expect = 0.001
Identities = 26/70 (37%), Positives = 40/70 (57%), Gaps = 1/70 (1%)
Frame = +2
Query: 65 LKGKVALITGAASGIGLAYSEELLKQG-AKVSLCDIDSERGEQVTEKLASKYGRKNVLFC 241
L GK LITGA+SGIG + + E+ K K+ L E++ ++L SKY +VL
Sbjct: 4 LDGKTILITGASSGIGKSTAFEIAKVAKVKLILAARRFSTVEEIAKELESKY-EVSVLPL 62
Query: 242 RLDVMTIHNL 271
+LDV + ++
Sbjct: 63 KLDVSDLKSI 72
Score = 26.2 bits (55), Expect = 4.2
Identities = 12/28 (42%), Positives = 19/28 (67%), Gaps = 2/28 (7%)
Frame = +1
Query: 295 EEFNRIDIVINNAGFM--NDRLWELEVD 372
+EF ID++INNAG D++ +L +D
Sbjct: 81 KEFADIDVLINNAGLALGTDKVIDLNID 108
>SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 258
Score = 37.9 bits (84), Expect = 0.001
Identities = 25/75 (33%), Positives = 39/75 (52%), Gaps = 6/75 (8%)
Frame = +1
Query: 265 QFEEAFVMTVEEFNRIDIVINNAGFMNDRLWEL----EVDVNLNGVIRGTLLAYRFM--G 426
Q E A ++ ID++INNAG +D +E D +++ V+RG L R++
Sbjct: 59 QIETAIEKVIQTLGPIDVLINNAGLADDTPFEQLSHESWDHDVSLVLRGNYLTQRYVIPH 118
Query: 427 KDRGGQGGVIVNTAS 471
+ G+GG IVN S
Sbjct: 119 MAKQGKGGSIVNIGS 133
Score = 32.3 bits (70), Expect = 0.064
Identities = 16/37 (43%), Positives = 23/37 (62%)
Frame = +2
Query: 59 MDLKGKVALITGAASGIGLAYSEELLKQGAKVSLCDI 169
M ++G+V LITGAA GIG + + G +V+ DI
Sbjct: 1 MTVEGRVVLITGAAGGIGKVLCKMFTELGDRVAGIDI 37
>SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 255
Score = 35.1 bits (77), Expect = 0.009
Identities = 17/52 (32%), Positives = 28/52 (53%)
Frame = +2
Query: 65 LKGKVALITGAASGIGLAYSEELLKQGAKVSLCDIDSERGEQVTEKLASKYG 220
LKGK LITG + GIG + ++ G+ V L +++ + +L K+G
Sbjct: 7 LKGKTTLITGGSGGIGFSIAKAFAAAGSNVGLLYGRNKKALEYAAELRDKHG 58
Score = 25.4 bits (53), Expect = 7.3
Identities = 24/70 (34%), Positives = 32/70 (45%), Gaps = 10/70 (14%)
Frame = +1
Query: 292 VEEFN-RIDIVINNAGFM---------NDRLWELEVDVNLNGVIRGTLLAYRFMGKDRGG 441
VEE R+D++I NAG N+ +W V +NLNG A K G
Sbjct: 82 VEELGGRLDVMIANAGIAIPHLSLEDKNEDIWTKVVGINLNGAYYTAQAAGHHFKKQ--G 139
Query: 442 QGGVIVNTAS 471
+G +I TAS
Sbjct: 140 KGSLIF-TAS 148
>SPAC22A12.17c |||short chain dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 261
Score = 33.9 bits (74), Expect = 0.021
Identities = 21/65 (32%), Positives = 33/65 (50%)
Frame = +2
Query: 59 MDLKGKVALITGAASGIGLAYSEELLKQGAKVSLCDIDSERGEQVTEKLASKYGRKNVLF 238
+ LKGK A++ G A GIG A + GA + ++ GE+ +++A G K
Sbjct: 17 LSLKGKNAVVFGGARGIGHAICSVFAEAGANAFIV-YNTTPGEKAAKEIAQANGVKTYT- 74
Query: 239 CRLDV 253
C+ DV
Sbjct: 75 CKCDV 79
>SPCC162.03 |||short chain dehydrogenase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 292
Score = 33.1 bits (72), Expect = 0.036
Identities = 30/92 (32%), Positives = 41/92 (44%), Gaps = 8/92 (8%)
Frame = +1
Query: 226 KRFVLSIRCYDYPQFEEAFVMTVEEFNRIDIVINNAG------FMNDRLWELEVDVNLN- 384
K L + D E AF F +DIVINNAG F + + E+ +N+N
Sbjct: 48 KLLKLKLDVTDVKSVETAFKDAKRRFGNVDIVINNAGYGLVGEFESYNIEEMHRQMNVNF 107
Query: 385 -GVIRGTLLAYRFMGKDRGGQGGVIVNTASTA 477
GV T A M + G+GG I+ +S A
Sbjct: 108 WGVAYITKEALNLMRE--SGKGGRILQISSVA 137
Score = 25.0 bits (52), Expect = 9.7
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = +2
Query: 83 LITGAASGIGLAYSEELLKQGAKVSLC 163
LITG++ G+G A + L QG V C
Sbjct: 9 LITGSSKGLGYALVKVGLAQGYNVIAC 35
>SPAC4G9.15 |||ketoreductase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 341
Score = 31.9 bits (69), Expect = 0.084
Identities = 16/46 (34%), Positives = 25/46 (54%)
Frame = +2
Query: 68 KGKVALITGAASGIGLAYSEELLKQGAKVSLCDIDSERGEQVTEKL 205
KG A++TGA GIG Y+ +L G V L E+ + + ++L
Sbjct: 56 KGYWAVVTGATDGIGKEYATQLAMSGFNVVLISRTQEKLDALAKEL 101
>SPAC4H3.08 |||short chain dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 286
Score = 31.1 bits (67), Expect = 0.15
Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 1/54 (1%)
Frame = +2
Query: 65 LKGKVALITGAASGIGLAYSEELLKQGAKVSLCDIDSERGE-QVTEKLASKYGR 223
L K L+TG SGIG A + ++G+ + + + ER + +VT L + GR
Sbjct: 40 LAEKKTLLTGGDSGIGKAAAVMFAREGSDLVISCLPEERDDAEVTRDLIEREGR 93
>SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase
Ubp4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 438
Score = 31.1 bits (67), Expect = 0.15
Identities = 21/74 (28%), Positives = 33/74 (44%)
Frame = +3
Query: 393 QRYAARISVYGEGSRRARRCHREHGLYSICETSSVDSDLTATNYAIVGLTRAYGDQYHVN 572
+RY +S+ + R + + VDS L+ NY +GLT G+ ++N
Sbjct: 26 ERYFEDVSLAYKLYSMLRSLKLDPHFMELANPKKVDSSLSYENYQPIGLTN-LGNTCYMN 84
Query: 573 MTGLRCLVVCPGLT 614
L+CL C LT
Sbjct: 85 CV-LQCLFACKDLT 97
>SPCC1739.08c |||short chain dehydrogenase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 261
Score = 31.1 bits (67), Expect = 0.15
Identities = 17/52 (32%), Positives = 29/52 (55%)
Frame = +2
Query: 65 LKGKVALITGAASGIGLAYSEELLKQGAKVSLCDIDSERGEQVTEKLASKYG 220
LKGK ++ GAA GIG + + + G V + + ++ E+ +KLA + G
Sbjct: 19 LKGKNCVVFGAAKGIGFSIATAFAQAGGNVIITYLTTDPTEK-AKKLAEETG 69
>SPAC19A8.06 |||short chain dehydrogenase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 397
Score = 30.3 bits (65), Expect = 0.26
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +2
Query: 65 LKGKVALITGAASGIGLAYSEELLKQGAKV 154
+ G V ++TG +SGIG E+L GA+V
Sbjct: 73 MNGMVVMVTGGSSGIGQVVVEKLASLGAQV 102
>SPBC30D10.05c |||sepiapterin reductase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 247
Score = 29.9 bits (64), Expect = 0.34
Identities = 16/27 (59%), Positives = 21/27 (77%)
Frame = +2
Query: 74 KVALITGAASGIGLAYSEELLKQGAKV 154
KV L+TG++ GIGLA +E L K+ AKV
Sbjct: 7 KVILLTGSSKGIGLATAEALQKK-AKV 32
>SPCC1450.15 |||pig-F |Schizosaccharomyces pombe|chr 3|||Manual
Length = 503
Score = 29.1 bits (62), Expect = 0.59
Identities = 11/26 (42%), Positives = 20/26 (76%)
Frame = +2
Query: 83 LITGAASGIGLAYSEELLKQGAKVSL 160
L+TG + G+G A ++EL+ +GA V++
Sbjct: 26 LVTGGSQGLGKAIAKELVLRGANVTI 51
>SPAC513.07 |||flavonol reductase/cinnamoyl-CoA reductase
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 336
Score = 28.7 bits (61), Expect = 0.78
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +2
Query: 65 LKGKVALITGAASGIGLAYSEELLKQGAKV 154
+ GK+ L+TG IG +E+LL+ G +V
Sbjct: 1 MSGKLVLVTGVTGFIGAHVAEQLLQAGYRV 30
>SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 937
Score = 27.9 bits (59), Expect = 1.4
Identities = 14/47 (29%), Positives = 25/47 (53%)
Frame = +2
Query: 38 CFYSSINMDLKGKVALITGAASGIGLAYSEELLKQGAKVSLCDIDSE 178
C ++++GK A++ G ++ +G S L K A V+LC +E
Sbjct: 150 CILKHYGINVQGKHAVVIGRSNIVGRPMSILLEKANATVTLCHSKTE 196
>SPBC1703.05 |||protein kinase, RIO family|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 336
Score = 27.5 bits (58), Expect = 1.8
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +1
Query: 256 DYPQFEEAFVMTVEEFNRIDIVINNAGFMNDRLWELE 366
D P FE+ + +E+ N +DI+I +GF + ELE
Sbjct: 274 DVPNFED--ISKMEKENNLDIMIEASGFNKKQSKELE 308
>SPBC342.01c |alg6|SPBC3F6.06c|glucosyltransferase
Alg6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 506
Score = 27.1 bits (57), Expect = 2.4
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = -3
Query: 345 VHEASIINDNVNPVELLYCHHKCLFKLWIVITSN 244
VHE S++ + P +L CH K WI + +N
Sbjct: 362 VHEKSVLLPLL-PTSILLCHGNITTKPWIALANN 394
>SPAC977.08 |||short chain dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 236
Score = 27.1 bits (57), Expect = 2.4
Identities = 8/28 (28%), Positives = 18/28 (64%)
Frame = +1
Query: 256 DYPQFEEAFVMTVEEFNRIDIVINNAGF 339
++ +++ + F RID+++NNAG+
Sbjct: 25 NFSSIKKSVEKAISHFGRIDVLLNNAGY 52
>SPBC1348.09 |||short chain dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 236
Score = 27.1 bits (57), Expect = 2.4
Identities = 8/28 (28%), Positives = 18/28 (64%)
Frame = +1
Query: 256 DYPQFEEAFVMTVEEFNRIDIVINNAGF 339
++ +++ + F RID+++NNAG+
Sbjct: 25 NFSSIKKSVEKAISHFGRIDVLLNNAGY 52
>SPBC16E9.14c |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 386
Score = 26.6 bits (56), Expect = 3.2
Identities = 21/94 (22%), Positives = 43/94 (45%), Gaps = 5/94 (5%)
Frame = -3
Query: 444 LPSAILPHKPICEQRTSDDAIQINIDFEFPKPVVHEASIINDNVNPVELLYCHHK-CLFK 268
+P+AI PI ++ + + I + PKP + ++++ + P HHK L
Sbjct: 7 IPTAIQIQNPI------NNNVSVTISDQLPKPSANNPNLLSVDTRPTHRKGHHHKHSLSH 60
Query: 267 LWIVITSNRQ----NKTFFRPYFEASFSVTCSPR 178
+ + NRQ ++ P F+ +F++ P+
Sbjct: 61 QYFLPPKNRQPLEIPASYPIPTFKETFAILTFPQ 94
>SPBC17A3.07 |pgr1||glutathione reductase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 464
Score = 26.2 bits (55), Expect = 4.2
Identities = 14/34 (41%), Positives = 18/34 (52%)
Frame = +2
Query: 83 LITGAASGIGLAYSEELLKQGAKVSLCDIDSERG 184
L+ G SG GLA + K GAKV+L + G
Sbjct: 11 LVIGGGSG-GLASARRAAKHGAKVALIEASGRLG 43
>SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2280
Score = 25.8 bits (54), Expect = 5.5
Identities = 11/30 (36%), Positives = 16/30 (53%)
Frame = +3
Query: 540 TRAYGDQYHVNMTGLRCLVVCPGLTDSGLV 629
+R+ YH+ + G RC LTD GL+
Sbjct: 645 SRSSPGSYHLFLNGSRCTAGVRSLTDGGLL 674
>SPAC29A4.15 |||serine-tRNA ligase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 450
Score = 25.8 bits (54), Expect = 5.5
Identities = 12/40 (30%), Positives = 20/40 (50%)
Frame = -1
Query: 635 ILHQTTVRESRAHDEASQSGHIDVVLIPVSSGEANNSVVR 516
++ Q T + + S G+I +PVS E NN ++R
Sbjct: 90 LIEQETAKNKEMLNVVSSIGNIVHDSVPVSMDEDNNEIIR 129
>SPCC63.08c |ppk36|atg1|serine/threonine protein kinase
Ppk36|Schizosaccharomyces pombe|chr 3|||Manual
Length = 830
Score = 25.4 bits (53), Expect = 7.3
Identities = 14/42 (33%), Positives = 22/42 (52%), Gaps = 3/42 (7%)
Frame = -3
Query: 177 SESMSHRETL---APCFNNSSL*AKPMPEAAPVINATFPFKS 61
+ES++H +++ +P N PE+APV N FP S
Sbjct: 452 NESLTHEQSINGNSPSPNEGVFQGSFSPESAPVNNHAFPHTS 493
>SPAC6F12.02 |rst2||transcription factor Rst2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 567
Score = 25.4 bits (53), Expect = 7.3
Identities = 15/41 (36%), Positives = 19/41 (46%), Gaps = 1/41 (2%)
Frame = +2
Query: 461 TRPLQHLRDLKCRLRSHSNELRY-CWPHQSLRGSVPRQYDR 580
TR L LK +RSH+NE + C L RQ+ R
Sbjct: 76 TRAFARLEHLKRHIRSHTNEKPFTCSEIDGLPTGCGRQFSR 116
>SPAC24C9.14 |otu1|mug141|ubiquitin-specific protease
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 329
Score = 25.4 bits (53), Expect = 7.3
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = -2
Query: 658 EDISCLSRSFTKPLSVSP 605
+D SCL R+ +KPL SP
Sbjct: 142 DDNSCLFRALSKPLGFSP 159
>SPBC1709.07 |erg27||3-keto sterol reductase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 338
Score = 25.0 bits (52), Expect = 9.7
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = +2
Query: 74 KVALITGAASGIGLAYSEELLK 139
K ALITG+ SG+G + LL+
Sbjct: 5 KYALITGSNSGLGFGIATRLLQ 26
>SPAC4H3.02c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 391
Score = 25.0 bits (52), Expect = 9.7
Identities = 15/41 (36%), Positives = 22/41 (53%), Gaps = 1/41 (2%)
Frame = +1
Query: 205 RFKIWPKKRFVLSIRCYDYPQFEEAFVMTVEEFNR-IDIVI 324
R K P+KR LSI D +F ++ + +E R DIV+
Sbjct: 249 RAKKPPRKRITLSINQQDKDEFFDSLINNKKEVRRHFDIVM 289
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,670,176
Number of Sequences: 5004
Number of extensions: 54820
Number of successful extensions: 177
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 299817502
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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