Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--1558X
         (478 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig...    81   1e-16
SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||...    43   2e-05
SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa...    42   6e-05
SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomy...    38   0.001
SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyc...    37   0.002
SPBP8B7.27 |mug30||ubiquitin-protein ligase E3|Schizosaccharomyc...    35   0.007
SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo...    33   0.017
SPAC1A6.05c |||triacylglycerol lipase|Schizosaccharomyces pombe|...    27   1.9  
SPAC19G12.01c |cut20|lid1, apc4, SPAPJ698.04c|anaphase-promoting...    25   4.5  

>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
            E3 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1647

 Score = 80.6 bits (190), Expect = 1e-16
 Identities = 38/81 (46%), Positives = 51/81 (62%)
 Frame = +2

Query: 20   GGRDQRWDPRMLAECIRPDHGYNAESRAIRMLIDILASYNREEQRHFLQFVTGSPRLPTG 199
            G  D+ W    L + I  DHGY  ES  I+ L+ +++  N +EQR FLQF+TGS +LP G
Sbjct: 1523 GTVDEDWSYATLMKSIVADHGYTMESPTIQRLLTLMSQMNFQEQRDFLQFITGSRKLPIG 1582

Query: 200  GFKALTPPLTVVRKSLESYWI 262
            GF  L PPLTVVR+  E  ++
Sbjct: 1583 GFAGLNPPLTVVRRLNEPPYV 1603



 Score = 72.5 bits (170), Expect = 3e-14
 Identities = 32/44 (72%), Positives = 38/44 (86%)
 Frame = +1

Query: 262  PDEYLPSVMTCVNYLKLPDYSSAEVMXAKLRLAASEGQHSFHLS 393
            PD+YLPSVMTCVNYLKLP+YSS+EV+ ++L  A  EGQ SFHLS
Sbjct: 1604 PDDYLPSVMTCVNYLKLPEYSSSEVLGSRLSKAILEGQGSFHLS 1647


>SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 3227

 Score = 43.2 bits (97), Expect = 2e-05
 Identities = 18/46 (39%), Positives = 27/46 (58%)
 Frame = +2

Query: 77   HGYNAESRAIRMLIDILASYNREEQRHFLQFVTGSPRLPTGGFKAL 214
            HGYN  S  ++     + S++ EE+   LQF TG+ ++P  GFK L
Sbjct: 3122 HGYNVSSPQVQWFWRAVRSFDEEERAKLLQFATGTSKVPLNGFKEL 3167



 Score = 33.5 bits (73), Expect = 0.017
 Identities = 15/37 (40%), Positives = 20/37 (54%)
 Frame = +1

Query: 274  LPSVMTCVNYLKLPDYSSAEVMXAKLRLAASEGQHSF 384
            LP   TC N L LP+Y + E + + L  A +EG   F
Sbjct: 3188 LPQSHTCFNQLDLPEYDTYEQLRSMLLTAINEGSEGF 3224


>SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1029

 Score = 41.5 bits (93), Expect = 6e-05
 Identities = 19/51 (37%), Positives = 28/51 (54%)
 Frame = +2

Query: 80   GYNAESRAIRMLIDILASYNREEQRHFLQFVTGSPRLPTGGFKALTPPLTV 232
            GY   S  I +  ++L  +  E++R F++FVT   R P  GFKAL P   +
Sbjct: 931  GYEPNSPTIVLFWEVLREFEEEDKRSFVKFVTSVARPPILGFKALMPSFCI 981



 Score = 35.5 bits (78), Expect = 0.004
 Identities = 16/33 (48%), Positives = 20/33 (60%)
 Frame = +1

Query: 274  LPSVMTCVNYLKLPDYSSAEVMXAKLRLAASEG 372
            LP+  TCVN LKLP YS+ + +  KL  A   G
Sbjct: 991  LPTASTCVNLLKLPMYSTKQTLRDKLLTAVRSG 1023


>SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 786

 Score = 37.5 bits (83), Expect = 0.001
 Identities = 16/44 (36%), Positives = 25/44 (56%)
 Frame = +2

Query: 83  YNAESRAIRMLIDILASYNREEQRHFLQFVTGSPRLPTGGFKAL 214
           Y A    I+   +I+A +  E++   LQF TG+ R+P  GF+ L
Sbjct: 685 YIATDPVIKWFWEIIAGWKNEDRSKLLQFATGTSRIPVNGFRDL 728



 Score = 34.3 bits (75), Expect = 0.010
 Identities = 17/33 (51%), Positives = 20/33 (60%)
 Frame = +1

Query: 262 PDEYLPSVMTCVNYLKLPDYSSAEVMXAKLRLA 360
           PD+ LP   TC N L LPDY S + +  KL LA
Sbjct: 745 PDQ-LPVAHTCFNRLDLPDYPSKDTLHEKLSLA 776


>SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 767

 Score = 36.7 bits (81), Expect = 0.002
 Identities = 14/44 (31%), Positives = 26/44 (59%)
 Frame = +2

Query: 83  YNAESRAIRMLIDILASYNREEQRHFLQFVTGSPRLPTGGFKAL 214
           Y+   + I+   +++  ++ E++   LQF TG+ R+P  GFK L
Sbjct: 666 YSENDQIIKWFWELMDEWSNEKKSRLLQFTTGTSRIPVNGFKDL 709



 Score = 30.7 bits (66), Expect = 0.12
 Identities = 14/32 (43%), Positives = 18/32 (56%)
 Frame = +1

Query: 274 LPSVMTCVNYLKLPDYSSAEVMXAKLRLAASE 369
           LP   TC N L LP Y+S + +  KL +A  E
Sbjct: 729 LPKAHTCFNRLDLPPYTSKKDLDHKLSIAVEE 760


>SPBP8B7.27 |mug30||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 807

 Score = 34.7 bits (76), Expect = 0.007
 Identities = 15/42 (35%), Positives = 26/42 (61%)
 Frame = +2

Query: 77  HGYNAESRAIRMLIDILASYNREEQRHFLQFVTGSPRLPTGG 202
           H + ++ + I    D+++ Y+ + Q+ FL FVTGS R+P  G
Sbjct: 710 HSFVSKRKIILWFWDLISHYSLKMQKLFLIFVTGSDRIPATG 751



 Score = 29.5 bits (63), Expect = 0.28
 Identities = 17/40 (42%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
 Frame = +1

Query: 253 LLDPD-EYLPSVMTCVNYLKLPDYSSAEVMXAKLRLAASE 369
           +L PD + LP   TC N+L + +YSS E +  KL  A  E
Sbjct: 761 VLGPDSDQLPISHTCFNHLCIWEYSSREKLKKKLDTALLE 800


>SPAC1805.15c |pub2||ubiquitin-protein ligase
           Pub2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 671

 Score = 33.5 bits (73), Expect = 0.017
 Identities = 13/30 (43%), Positives = 21/30 (70%)
 Frame = +2

Query: 119 DILASYNREEQRHFLQFVTGSPRLPTGGFK 208
           ++L+ ++ E++   LQF TG+ RLP  GFK
Sbjct: 582 ELLSEWSPEKKAKLLQFATGTSRLPLSGFK 611



 Score = 31.9 bits (69), Expect = 0.052
 Identities = 14/32 (43%), Positives = 18/32 (56%)
 Frame = +1

Query: 274 LPSVMTCVNYLKLPDYSSAEVMXAKLRLAASE 369
           LP   TC N L +P Y+S E +  KL +A  E
Sbjct: 633 LPKAHTCFNRLDIPPYNSKEELEQKLTIAIQE 664


>SPAC1A6.05c |||triacylglycerol lipase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 483

 Score = 26.6 bits (56), Expect = 1.9
 Identities = 15/44 (34%), Positives = 23/44 (52%), Gaps = 1/44 (2%)
 Frame = +1

Query: 265 DEYLPSVMTCVNYL-KLPDYSSAEVMXAKLRLAASEGQHSFHLS 393
           DE++  V+ C+ YL + PD S  E +    RL  + G  +  LS
Sbjct: 101 DEFIQEVLMCLTYLEETPDLSLDEKITEFSRLKLTTGNTALILS 144


>SPAC19G12.01c |cut20|lid1, apc4, SPAPJ698.04c|anaphase-promoting
           complex subunit Apc4|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 719

 Score = 25.4 bits (53), Expect = 4.5
 Identities = 11/36 (30%), Positives = 19/36 (52%)
 Frame = +1

Query: 199 WIQGIDSTTNCGA*IAGVLLDPDEYLPSVMTCVNYL 306
           WI   +     G+ +  V+L P   L +++TC N+L
Sbjct: 16  WINETNRVERVGSGLKRVILCPSMELIAILTCSNHL 51


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,051,572
Number of Sequences: 5004
Number of extensions: 39608
Number of successful extensions: 110
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 100
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 110
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 184476110
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)