Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1558X
(478 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g38600.1 68417.m05464 HECT-domain-containing protein / ubiqui... 84 4e-17
At4g38600.2 68417.m05463 HECT-domain-containing protein / ubiqui... 79 2e-15
At5g02880.1 68418.m00231 HECT-domain-containing protein / ubiqui... 76 1e-14
At3g17205.1 68416.m02196 HECT-domain-containing protein / ubiqui... 46 2e-05
At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearl... 44 7e-05
At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearl... 43 9e-05
At3g53090.1 68416.m05851 HECT-domain-containing protein / ubiqui... 39 0.002
At4g12570.1 68417.m01983 ubiquitin-protein ligase, putative simi... 33 0.100
At5g57500.1 68418.m07185 expressed protein 30 0.70
At5g05070.1 68418.m00538 zinc finger (DHHC type) family protein ... 28 3.8
At3g59770.1 68416.m06670 sacI homology domain-containing protein... 28 3.8
At1g64210.1 68414.m07274 leucine-rich repeat transmembrane prote... 28 3.8
At4g01500.1 68417.m00193 DNA-binding protein, putative similar t... 27 5.0
At2g01730.1 68415.m00101 metallo-beta-lactamase family protein s... 27 5.0
At5g18880.1 68418.m02243 expressed protein ; expression supporte... 27 8.7
At4g30130.1 68417.m04283 expressed protein contains Pfam domains... 27 8.7
At4g24610.1 68417.m03525 expressed protein 27 8.7
At3g07400.1 68416.m00882 lipase class 3 family protein contains ... 27 8.7
At1g77460.1 68414.m09020 C2 domain-containing protein / armadill... 27 8.7
At1g19270.1 68414.m02397 ubiquitin interaction motif-containing ... 27 8.7
>At4g38600.1 68417.m05464 HECT-domain-containing protein /
ubiquitin-transferase family protein similar to
SP|Q14669Thyroid receptor interacting protein 12 (TRIP12)
{Homo sapiens}; contains Pfam profile PF00632:
HECT-domain (ubiquitin-transferase)
Length = 1888
Score = 84.2 bits (199), Expect = 4e-17
Identities = 39/73 (53%), Positives = 50/73 (68%)
Frame = +2
Query: 23 GRDQRWDPRMLAECIRPDHGYNAESRAIRMLIDILASYNREEQRHFLQFVTGSPRLPTGG 202
GR + W+ LAE I+ DHGYNA+S AI L++I+ ++QR F QFVTG+PRLP GG
Sbjct: 1755 GRRELWEVETLAEHIKFDHGYNAKSPAIINLLEIMGELTADQQRAFCQFVTGAPRLPPGG 1814
Query: 203 FKALTPPLTVVRK 241
L P LT+VRK
Sbjct: 1815 LAVLNPKLTIVRK 1827
Score = 63.7 bits (148), Expect = 6e-11
Identities = 33/59 (55%), Positives = 39/59 (66%)
Frame = +1
Query: 217 STTNCGA*IAGVLLDPDEYLPSVMTCVNYLKLPDYSSAEVMXAKLRLAASEGQHSFHLS 393
ST++ A AG D+ LPSVMTC NYLKLP YS+ E+M KL A +EGQ SF LS
Sbjct: 1830 STSSAAANGAGASETADDDLPSVMTCANYLKLPPYSTKEIMYKKLLYAINEGQGSFDLS 1888
>At4g38600.2 68417.m05463 HECT-domain-containing protein /
ubiquitin-transferase family protein similar to
SP|Q14669Thyroid receptor interacting protein 12 (TRIP12)
{Homo sapiens}; contains Pfam profile PF00632:
HECT-domain (ubiquitin-transferase)
Length = 1794
Score = 79.0 bits (186), Expect = 2e-15
Identities = 39/73 (53%), Positives = 49/73 (67%)
Frame = +2
Query: 23 GRDQRWDPRMLAECIRPDHGYNAESRAIRMLIDILASYNREEQRHFLQFVTGSPRLPTGG 202
GR + W+ LAE I+ DHGYNA+S AI I+I+ ++QR F QFVTG+PRLP GG
Sbjct: 1664 GRRELWEVETLAEHIKFDHGYNAKSPAI---INIMGELTADQQRAFCQFVTGAPRLPPGG 1720
Query: 203 FKALTPPLTVVRK 241
L P LT+VRK
Sbjct: 1721 LAVLNPKLTIVRK 1733
Score = 63.7 bits (148), Expect = 6e-11
Identities = 33/59 (55%), Positives = 39/59 (66%)
Frame = +1
Query: 217 STTNCGA*IAGVLLDPDEYLPSVMTCVNYLKLPDYSSAEVMXAKLRLAASEGQHSFHLS 393
ST++ A AG D+ LPSVMTC NYLKLP YS+ E+M KL A +EGQ SF LS
Sbjct: 1736 STSSAAANGAGASETADDDLPSVMTCANYLKLPPYSTKEIMYKKLLYAINEGQGSFDLS 1794
>At5g02880.1 68418.m00231 HECT-domain-containing protein /
ubiquitin-transferase family protein /
armadillo/beta-catenin-like repeat-containing protein
similar to SP|Q14669 Thyroid receptor interacting protein
12 (TRIP12) {Homo sapiens}; contains Pfam profiles
PF00632: HECT-domain (ubiquitin-transferase), PF00514:
Armadillo/beta-catenin-like repeat
Length = 1502
Score = 76.2 bits (179), Expect = 1e-14
Identities = 34/59 (57%), Positives = 44/59 (74%)
Frame = +2
Query: 65 IRPDHGYNAESRAIRMLIDILASYNREEQRHFLQFVTGSPRLPTGGFKALTPPLTVVRK 241
I+ DHGY + S + L+ IL ++RE+QR FLQFVTGSPRLP GG +L+P LT+VRK
Sbjct: 1395 IKFDHGYTSSSPPVEYLLQILHEFDREQQRAFLQFVTGSPRLPHGGLASLSPKLTIVRK 1453
Score = 60.9 bits (141), Expect = 4e-10
Identities = 30/43 (69%), Positives = 31/43 (72%)
Frame = +1
Query: 265 DEYLPSVMTCVNYLKLPDYSSAEVMXAKLRLAASEGQHSFHLS 393
D LPSVMTC NYLKLP YSS E M KL A +EGQ SFHLS
Sbjct: 1460 DTDLPSVMTCANYLKLPPYSSKEKMKEKLIYAITEGQGSFHLS 1502
>At3g17205.1 68416.m02196 HECT-domain-containing protein /
ubiquitin-transferase family protein weak similarity to
ubiquitin-protein ligase 2 [Arabidopsis thaliana]
GI:7108523; contains Pfam profile PF00632: HECT-domain
(ubiquitin-transferase)
Length = 873
Score = 45.6 bits (103), Expect = 2e-05
Identities = 22/53 (41%), Positives = 31/53 (58%)
Frame = +2
Query: 80 GYNAESRAIRMLIDILASYNREEQRHFLQFVTGSPRLPTGGFKALTPPLTVVR 238
GY+A I M +++ S++ E Q+ FL+FVTG R P GFK L P + R
Sbjct: 770 GYHAGHYVIDMFWEVMKSFSTENQKKFLKFVTGCSRGPLLGFKYLEPAFCIQR 822
Score = 36.7 bits (81), Expect = 0.008
Identities = 19/40 (47%), Positives = 24/40 (60%)
Frame = +1
Query: 274 LPSVMTCVNYLKLPDYSSAEVMXAKLRLAASEGQHSFHLS 393
LP+ TC+N LKLP Y S E++ KL A S + F LS
Sbjct: 835 LPTSATCMNLLKLPPYQSKELLETKLMYAIS-AEAGFDLS 873
>At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearly
identical to ubiquitin-protein ligase 2 [Arabidopsis
thaliana] GI:7108523; E3, HECT-domain protein family;
similar to ubiquitin-protein ligase 2 GI:7108523 from
[Arabidopsis thaliana]
Length = 3658
Score = 43.6 bits (98), Expect = 7e-05
Identities = 18/44 (40%), Positives = 29/44 (65%)
Frame = +2
Query: 83 YNAESRAIRMLIDILASYNREEQRHFLQFVTGSPRLPTGGFKAL 214
Y S IR +++ ++++E+ FLQFVTG+ ++P GFKAL
Sbjct: 3555 YTVGSPVIRWFWEVVKAFSKEDMARFLQFVTGTSKVPLEGFKAL 3598
Score = 37.5 bits (83), Expect = 0.005
Identities = 18/39 (46%), Positives = 21/39 (53%)
Frame = +1
Query: 268 EYLPSVMTCVNYLKLPDYSSAEVMXAKLRLAASEGQHSF 384
E LPS TC N L LP+Y S E + +L LA E F
Sbjct: 3617 ERLPSAHTCFNQLDLPEYQSKEQVQERLLLAIHEANEGF 3655
>At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearly
identical to ubiquitin-protein ligase 1 [Arabidopsis
thaliana] GI:7108521; E3, HECT-domain protein family;
similar to GI:7108521, GB:AAF36454 from [Arabidopsis
thaliana]
Length = 3891
Score = 43.2 bits (97), Expect = 9e-05
Identities = 18/44 (40%), Positives = 29/44 (65%)
Frame = +2
Query: 83 YNAESRAIRMLIDILASYNREEQRHFLQFVTGSPRLPTGGFKAL 214
Y A S I +++ ++++E+ FLQFVTG+ ++P GFKAL
Sbjct: 3788 YTAGSPVIHWFWEVVKAFSKEDMARFLQFVTGTSKVPLEGFKAL 3831
Score = 37.9 bits (84), Expect = 0.004
Identities = 18/39 (46%), Positives = 21/39 (53%)
Frame = +1
Query: 268 EYLPSVMTCVNYLKLPDYSSAEVMXAKLRLAASEGQHSF 384
E LPS TC N L LP+Y S E + +L LA E F
Sbjct: 3850 ERLPSAHTCFNQLDLPEYQSKEQLQERLLLAIHEASEGF 3888
>At3g53090.1 68416.m05851 HECT-domain-containing protein /
ubiquitin-transferase family protein / IQ
calmodulin-binding motif-containing protein contains Pfam
profiles PF00632: HECT-domain (ubiquitin-transferase),
PF00612: IQ calmodulin-binding motif
Length = 1142
Score = 39.1 bits (87), Expect = 0.002
Identities = 20/61 (32%), Positives = 32/61 (52%), Gaps = 1/61 (1%)
Frame = +2
Query: 80 GYNAESRAIRMLIDILASYNREEQRHFLQFVTGSPRLPTGGFKALTPPLTVVRKSLE-SY 256
GY+ SR I++ +++ + E+ L+FVT R P GFK L P + + S + S
Sbjct: 1033 GYSDSSRTIKIFWEVMKGFEPSERCLLLKFVTSCSRAPLLGFKYLQPTFIIHKVSCDTSL 1092
Query: 257 W 259
W
Sbjct: 1093 W 1093
Score = 35.9 bits (79), Expect = 0.014
Identities = 21/42 (50%), Positives = 21/42 (50%)
Frame = +1
Query: 268 EYLPSVMTCVNYLKLPDYSSAEVMXAKLRLAASEGQHSFHLS 393
E LPS TC N LKLP Y A M KL L A F LS
Sbjct: 1102 ERLPSASTCYNTLKLPTYKRASTMREKL-LYAITSNAGFELS 1142
>At4g12570.1 68417.m01983 ubiquitin-protein ligase, putative similar
to SP|P39940 Ubiquitin--protein ligase RSP5 (EC 6.3.2.-)
{Saccharomyces cerevisiae}; contains Pfam profiles
PF00240: Ubiquitin family, PF00632: HECT-domain
(ubiquitin-transferase)
Length = 873
Score = 33.1 bits (72), Expect = 0.100
Identities = 18/54 (33%), Positives = 25/54 (46%)
Frame = +2
Query: 77 HGYNAESRAIRMLIDILASYNREEQRHFLQFVTGSPRLPTGGFKALTPPLTVVR 238
+G+ R I IL EEQR L F T + +P GF+ L+ L + R
Sbjct: 772 NGFKETDRQIDWFWKILKKMTEEEQRSILFFWTSNKFVPVEGFRGLSSKLYIYR 825
Score = 26.6 bits (56), Expect = 8.7
Identities = 13/39 (33%), Positives = 21/39 (53%)
Frame = +1
Query: 253 LLDPDEYLPSVMTCVNYLKLPDYSSAEVMXAKLRLAASE 369
L + ++ LP TC L +P Y + +M +LRL A +
Sbjct: 826 LYEANDRLPLSHTCFYRLCIPRYPTITLMEQRLRLIAQD 864
>At5g57500.1 68418.m07185 expressed protein
Length = 318
Score = 30.3 bits (65), Expect = 0.70
Identities = 13/32 (40%), Positives = 21/32 (65%)
Frame = +2
Query: 104 IRMLIDILASYNREEQRHFLQFVTGSPRLPTG 199
IR+LI IL ++ +RHFL+ + G+ +P G
Sbjct: 69 IRILIGILTLPDQYSRRHFLRMIYGTQNVPDG 100
>At5g05070.1 68418.m00538 zinc finger (DHHC type) family protein
contains Pfam profile PF01529: DHHC zinc finger domain
Length = 339
Score = 27.9 bits (59), Expect = 3.8
Identities = 9/21 (42%), Positives = 14/21 (66%)
Frame = -1
Query: 250 LQRFTHHS*WWSQCLESTSWQ 188
+QRF HH W QC+ T+++
Sbjct: 194 VQRFDHHCPWVGQCIARTTYE 214
>At3g59770.1 68416.m06670 sacI homology domain-containing protein / WW
domain-containing protein contains Pfam profiles PF00397:
WW domain, PF02383: SacI homology domain; identical to
cDNA SAC domain protein 9 (SAC9) GI:31415734
Length = 1630
Score = 27.9 bits (59), Expect = 3.8
Identities = 17/45 (37%), Positives = 24/45 (53%)
Frame = -2
Query: 237 RTTVSGGVNALNPPVGSLGLPVTNCKKCRCSSRLYEAKISISMRI 103
R T +GG + + S+G CKKC CSS + EA I +R+
Sbjct: 1136 RDTANGGGSLADVSATSIGSDHYICKKC-CSSIVLEALIVDYVRV 1179
>At1g64210.1 68414.m07274 leucine-rich repeat transmembrane protein
kinase, putative contains 1 predicted transmembrane
domain; similar to receptor-like protein kinase
(GI:4008006) [Arabidopsis thaliana]; similar to
receptor-like kinase RHG1 (GI:21239382) [Glycine max];
similar to receptor-like protein kinase 3 (GI:13506810)
[Lycopersicon esculentum]
Length = 587
Score = 27.9 bits (59), Expect = 3.8
Identities = 15/32 (46%), Positives = 18/32 (56%), Gaps = 1/32 (3%)
Frame = +2
Query: 179 SPRLPTGGFKALTPPLTVVR-KSLESYWIRMN 271
S RLP GF L PP T+ R SL+ +R N
Sbjct: 67 SVRLPAVGFNGLIPPFTISRLSSLKFLSLRKN 98
>At4g01500.1 68417.m00193 DNA-binding protein, putative similar to
DNA-binding proteins from [Arabidopsis thaliana] RAV1
GI:3868857, RAV2 GI:3868859; contains Pfam profile
PF02362: B3 DNA binding domain
Length = 328
Score = 27.5 bits (58), Expect = 5.0
Identities = 20/65 (30%), Positives = 27/65 (41%), Gaps = 2/65 (3%)
Frame = +2
Query: 71 PDHGYNAESRAIRMLIDIL--ASYNREEQRHFLQFVTGSPRLPTGGFKALTPPLTVVRKS 244
PD E R M ID A N + FV GSP PT F +TP ++ +S
Sbjct: 120 PDDNAQPERRRKIMFIDWRPRAEINFVHNINNHNFVFGSPTYPTARFYPVTPEYSMPYRS 179
Query: 245 LESYW 259
++
Sbjct: 180 FPPFY 184
>At2g01730.1 68415.m00101 metallo-beta-lactamase family protein
simliar to SP|P79101 Cleavage and polyadenylation
specificity factor, 73 kDa subunit (CPSF 73 kDa subunit)
{Bos taurus}; contains Pfam profile PF00753:
Metallo-beta-lactamase superfamily
Length = 613
Score = 27.5 bits (58), Expect = 5.0
Identities = 22/54 (40%), Positives = 26/54 (48%), Gaps = 5/54 (9%)
Frame = +2
Query: 128 ASYNREEQRHFLQFVTGSPRLPTGGFKALTPPLTVVRKS-----LESYWIRMNI 274
+ Y RE R FLQ V + GG KAL P + R L+ YW RMNI
Sbjct: 212 SKYPRE--REFLQAVH---KCVAGGGKALIPSFALGRAQELCMLLDDYWERMNI 260
>At5g18880.1 68418.m02243 expressed protein ; expression supported
by MPSS
Length = 295
Score = 26.6 bits (56), Expect = 8.7
Identities = 14/42 (33%), Positives = 18/42 (42%)
Frame = +1
Query: 1 VTRKSFRWT*PTMGSTYAGGMYSSGSWIQCRIAGYTHAY*YF 126
+T SF PT GM SW+ C THA+ +F
Sbjct: 143 ITWMSFLERLPTRDRLRGWGMNIPSSWVLCSNGDETHAHLFF 184
>At4g30130.1 68417.m04283 expressed protein contains Pfam domains,
PF04782: Protein of unknown function (DUF632) and
PF04783: Protein of unknown function (DUF630)
Length = 725
Score = 26.6 bits (56), Expect = 8.7
Identities = 18/62 (29%), Positives = 31/62 (50%), Gaps = 4/62 (6%)
Frame = +2
Query: 80 GYNAESRAIRMLIDILASYNREEQRHFLQF---VTGSPRLPT-GGFKALTPPLTVVRKSL 247
G+ A +++R + D L Y ++ H VT R+ + GGF ++PP +V+
Sbjct: 37 GHIAYIQSLRKVSDALREYIEGDEPHEFMLDTCVTPVKRMSSSGGFIEISPPSKMVQSEA 96
Query: 248 ES 253
ES
Sbjct: 97 ES 98
>At4g24610.1 68417.m03525 expressed protein
Length = 1145
Score = 26.6 bits (56), Expect = 8.7
Identities = 9/21 (42%), Positives = 15/21 (71%)
Frame = +3
Query: 135 TTVKNNDTSYSLLLEVQGCQL 197
T+++NN TSY ++ E C+L
Sbjct: 386 TSLRNNSTSYDIVQETYSCKL 406
>At3g07400.1 68416.m00882 lipase class 3 family protein contains
Pfam profile PF01764: Lipase
Length = 1003
Score = 26.6 bits (56), Expect = 8.7
Identities = 9/16 (56%), Positives = 14/16 (87%)
Frame = +3
Query: 6 AEVLQVDVTNDGIHVC 53
AE+L+VD+T G+H+C
Sbjct: 575 AELLKVDITGFGLHLC 590
>At1g77460.1 68414.m09020 C2 domain-containing protein /
armadillo/beta-catenin repeat family protein similar to
CCLS 65 [Silene latifolia] GI:2570102; contains Pfam
profiles PF00514: Armadillo/beta-catenin-like repeat,
PF00168: C2 domain
Length = 2110
Score = 26.6 bits (56), Expect = 8.7
Identities = 17/60 (28%), Positives = 31/60 (51%)
Frame = +3
Query: 6 AEVLQVDVTNDGIHVCWRNVFVRIMDTMQNRGLYACLLIFWLRTTVKNNDTSYSLLLEVQ 185
A L+V V + + +C +++ ++ + LL+ L +K+N TS+SL EVQ
Sbjct: 888 ASSLEVRVGSTALLLCAAKEKKQLITETLDQSGFLKLLLHALVDMIKHNSTSFSLETEVQ 947
>At1g19270.1 68414.m02397 ubiquitin interaction motif-containing
protein / LIM domain-containing protein weak similarity
to LIM-homeobox protein [Mus musculus] GI:2149584, Hic-5
[Mus musculus] GI:664955; contains Pfam profiles
PF02809: Ubiquitin interaction motif, PF00412: LIM
domain
Length = 532
Score = 26.6 bits (56), Expect = 8.7
Identities = 17/51 (33%), Positives = 25/51 (49%)
Frame = +3
Query: 33 NDGIHVCWRNVFVRIMDTMQNRGLYACLLIFWLRTTVKNNDTSYSLLLEVQ 185
NDG +C + +MDTMQ + LY + F+ +K LL+E Q
Sbjct: 288 NDGRKLCLECLDSAVMDTMQCQPLYLQIQNFYEGLNMKVEQEVPLLLVERQ 338
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,998,205
Number of Sequences: 28952
Number of extensions: 215954
Number of successful extensions: 582
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 557
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 581
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 821630280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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