BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--1540 (838 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_37259| Best HMM Match : No HMM Matches (HMM E-Value=.) 109 4e-24 SB_13681| Best HMM Match : Guanylate_cyc (HMM E-Value=2.8e-27) 83 4e-16 SB_29094| Best HMM Match : Guanylate_cyc (HMM E-Value=7.9e-07) 80 2e-15 SB_37135| Best HMM Match : Guanylate_cyc (HMM E-Value=0) 80 3e-15 SB_50980| Best HMM Match : Guanylate_cyc (HMM E-Value=0) 64 2e-10 SB_38875| Best HMM Match : No HMM Matches (HMM E-Value=.) 59 4e-09 SB_43991| Best HMM Match : No HMM Matches (HMM E-Value=.) 58 1e-08 SB_11488| Best HMM Match : No HMM Matches (HMM E-Value=.) 57 2e-08 SB_51990| Best HMM Match : Guanylate_cyc (HMM E-Value=0) 54 1e-07 SB_50731| Best HMM Match : Guanylate_cyc (HMM E-Value=0.76) 49 5e-06 SB_10830| Best HMM Match : No HMM Matches (HMM E-Value=.) 45 7e-05 SB_6162| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 2.7 SB_2034| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 2.7 >SB_37259| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1690 Score = 109 bits (261), Expect = 4e-24 Identities = 48/84 (57%), Positives = 63/84 (75%) Frame = +1 Query: 1 AKHISLLALDMMDLSQTVTVDGEPVGITIGIHSGEVVTGVIGHRMPRYCLFGNTVNLTSR 180 A+ I +ALDM ++Q++ G+ + I IGIHSGEVV GV+GH+ PRYCLFGNTVNLTSR Sbjct: 1533 ARSICNMALDMKHVTQSIIHKGDKIQIKIGIHSGEVVAGVVGHKTPRYCLFGNTVNLTSR 1592 Query: 181 CETTGVPGTINVSEDTYNYLMRDD 252 ETTG PG +N++E TY L+ + Sbjct: 1593 TETTGAPGQVNITEFTYRCLLEPE 1616 Score = 103 bits (248), Expect = 1e-22 Identities = 49/77 (63%), Positives = 60/77 (77%) Frame = +1 Query: 1 AKHISLLALDMMDLSQTVTVDGEPVGITIGIHSGEVVTGVIGHRMPRYCLFGNTVNLTSR 180 A +I+ LALDM+D+S+ + G + +TIGIHSGEVV GV+G + PRYCLFGNTVNLTSR Sbjct: 985 ALNIANLALDMIDISKGMKAKGIQLQVTIGIHSGEVVAGVVGQKKPRYCLFGNTVNLTSR 1044 Query: 181 CETTGVPGTINVSEDTY 231 ETTG+ G INVSE Y Sbjct: 1045 TETTGLKGKINVSEYAY 1061 Score = 78.2 bits (184), Expect = 8e-15 Identities = 33/78 (42%), Positives = 55/78 (70%), Gaps = 1/78 (1%) Frame = +1 Query: 1 AKHISLLALDMMD-LSQTVTVDGEPVGITIGIHSGEVVTGVIGHRMPRYCLFGNTVNLTS 177 A H++L+A+ M+D + ++ +G P+ + +GIH+G V++GV+G +MPRYCLFG+ V L + Sbjct: 528 AYHVALMAVGMIDHIRDVLSPEGVPLKMRVGIHTGSVLSGVVGTKMPRYCLFGHDVTLAN 587 Query: 178 RCETTGVPGTINVSEDTY 231 + E+ G IN+S TY Sbjct: 588 KMESESEAGRINISPTTY 605 Score = 33.1 bits (72), Expect = 0.29 Identities = 15/28 (53%), Positives = 17/28 (60%) Frame = +3 Query: 258 DDQFELTYRGHVTMKGKAEPMQTWFLTR 341 D F RG V MKG+ EPM T+ LTR Sbjct: 1619 DPSFVFERRGPVAMKGRQEPMVTYLLTR 1646 >SB_13681| Best HMM Match : Guanylate_cyc (HMM E-Value=2.8e-27) Length = 517 Score = 82.6 bits (195), Expect = 4e-16 Identities = 36/74 (48%), Positives = 53/74 (71%), Gaps = 4/74 (5%) Frame = +1 Query: 1 AKHISLLALDMMDLSQTVTVDGEPVG----ITIGIHSGEVVTGVIGHRMPRYCLFGNTVN 168 A I ++LD++ L++T T+D P G + +GIH+G V+GV+G++MPRYCLFG+TVN Sbjct: 390 ADEIGRMSLDLVRLTETTTLDSVPKGKKLMVRVGIHTGPCVSGVVGYKMPRYCLFGDTVN 449 Query: 169 LTSRCETTGVPGTI 210 SR +TTG+P I Sbjct: 450 TASRMQTTGIPNVI 463 >SB_29094| Best HMM Match : Guanylate_cyc (HMM E-Value=7.9e-07) Length = 235 Score = 80.2 bits (189), Expect = 2e-15 Identities = 36/62 (58%), Positives = 45/62 (72%) Frame = +1 Query: 58 VDGEPVGITIGIHSGEVVTGVIGHRMPRYCLFGNTVNLTSRCETTGVPGTINVSEDTYNY 237 VD EP+ I IGIH+G V+ GV+G +MPRYCLFGNTV L ++ E+ PG IN+S DT Sbjct: 11 VDREPLKIRIGIHTGPVMPGVVGKKMPRYCLFGNTVTLANKMESGSQPGRINISIDTKRL 70 Query: 238 LM 243 LM Sbjct: 71 LM 72 >SB_37135| Best HMM Match : Guanylate_cyc (HMM E-Value=0) Length = 584 Score = 79.8 bits (188), Expect = 3e-15 Identities = 39/85 (45%), Positives = 57/85 (67%), Gaps = 3/85 (3%) Frame = +1 Query: 1 AKHISLLALDMMDLSQTVTVDG---EPVGITIGIHSGEVVTGVIGHRMPRYCLFGNTVNL 171 A+H++ +AL+M+ + + + I IGIH+G VV GV+G +MPRYCLFG+TVN Sbjct: 479 AEHVADMALNMLSAMPEIEDPAGLNDHLRIRIGIHTGTVVAGVVGLKMPRYCLFGDTVNT 538 Query: 172 TSRCETTGVPGTINVSEDTYNYLMR 246 SR E+TG+ I+VSEDT +L + Sbjct: 539 ASRMESTGLAMAIHVSEDTQKFLAK 563 >SB_50980| Best HMM Match : Guanylate_cyc (HMM E-Value=0) Length = 313 Score = 63.7 bits (148), Expect = 2e-10 Identities = 33/69 (47%), Positives = 46/69 (66%), Gaps = 4/69 (5%) Frame = +1 Query: 1 AKHISLLALDMMDLSQT-VTVDGEP---VGITIGIHSGEVVTGVIGHRMPRYCLFGNTVN 168 A I+ ++L ++D QT V +P + + +GIHSG VV GV+G+ MPRYCLFG+TVN Sbjct: 242 AGEIARMSLHLVDAVQTEFCVRHKPEYKLKLRVGIHSGPVVAGVVGNTMPRYCLFGDTVN 301 Query: 169 LTSRCETTG 195 SR E+ G Sbjct: 302 TASRMESNG 310 >SB_38875| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1116 Score = 59.3 bits (137), Expect = 4e-09 Identities = 28/69 (40%), Positives = 38/69 (55%) Frame = +1 Query: 46 QTVTVDGEPVGITIGIHSGEVVTGVIGHRMPRYCLFGNTVNLTSRCETTGVPGTINVSED 225 +T E + GI G +V GVIG + P Y ++GNTVN+ SR ETTG G I ++ Sbjct: 1010 ETTRFYSENDSVVFGICQGPIVAGVIGAKKPHYDIWGNTVNVASRMETTGKAGCIQITAQ 1069 Query: 226 TYNYLMRDD 252 TY + D Sbjct: 1070 TYETIKDKD 1078 Score = 49.6 bits (113), Expect = 3e-06 Identities = 20/54 (37%), Positives = 35/54 (64%) Frame = +1 Query: 73 VGITIGIHSGEVVTGVIGHRMPRYCLFGNTVNLTSRCETTGVPGTINVSEDTYN 234 + + +GIH+G V+ GV+G R ++ + N V L + E+ G+PG +++SE T N Sbjct: 616 LNMRVGIHTGMVLAGVLGQRKWQFEAWSNDVTLANHMESGGIPGRVHISEPTLN 669 >SB_43991| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1149 Score = 57.6 bits (133), Expect = 1e-08 Identities = 30/69 (43%), Positives = 44/69 (63%), Gaps = 4/69 (5%) Frame = +1 Query: 1 AKHISLLALDMMD-LSQTVTVDGEP---VGITIGIHSGEVVTGVIGHRMPRYCLFGNTVN 168 A I+ +AL ++D +S V +P + + +G H+G VV GV+G+ M RYCLFG+TVN Sbjct: 946 AGEIAKMALHLVDAVSHNFIVRHKPEYKLQLRVGCHTGPVVAGVVGNTMARYCLFGDTVN 1005 Query: 169 LTSRCETTG 195 SR E+ G Sbjct: 1006 TASRMESNG 1014 >SB_11488| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 250 Score = 56.8 bits (131), Expect = 2e-08 Identities = 27/83 (32%), Positives = 47/83 (56%), Gaps = 3/83 (3%) Frame = +1 Query: 1 AKHISLLALDMMDLSQTVTVDGEP---VGITIGIHSGEVVTGVIGHRMPRYCLFGNTVNL 171 A I+ ++L +++ ++ P + +GIH+G V G++G + PRY +FG TVN+ Sbjct: 135 ASEIAAMSLHLLNYVTDFRIEHLPDTRLEFRMGIHTGPCVAGIVGLKKPRYDIFGETVNI 194 Query: 172 TSRCETTGVPGTINVSEDTYNYL 240 E+ G+P +I+VS T L Sbjct: 195 VKHLESAGIPQSIHVSSPTCRLL 217 >SB_51990| Best HMM Match : Guanylate_cyc (HMM E-Value=0) Length = 1055 Score = 54.4 bits (125), Expect = 1e-07 Identities = 23/75 (30%), Positives = 47/75 (62%), Gaps = 1/75 (1%) Frame = +1 Query: 19 LALDMMDLSQTVTVDGE-PVGITIGIHSGEVVTGVIGHRMPRYCLFGNTVNLTSRCETTG 195 + LDM++ + D + V + +G+H+G V+ G++G + ++ ++ N V L +R ET G Sbjct: 380 MGLDMLEQIKEFDNDTDNDVDMRVGVHTGTVLCGIVGTKRFKFDVWSNDVTLANRMETAG 439 Query: 196 VPGTINVSEDTYNYL 240 +PG ++++E T +L Sbjct: 440 LPGRVHITEATVGFL 454 Score = 52.0 bits (119), Expect = 6e-07 Identities = 26/54 (48%), Positives = 34/54 (62%) Frame = +1 Query: 79 ITIGIHSGEVVTGVIGHRMPRYCLFGNTVNLTSRCETTGVPGTINVSEDTYNYL 240 + IG ++G V GVIG Y ++G+TVNL SR ++TGVP I VSE T L Sbjct: 972 LRIGFNAGPVTAGVIGTTKLLYDIWGDTVNLASRMDSTGVPEKIQVSESTKEML 1025 >SB_50731| Best HMM Match : Guanylate_cyc (HMM E-Value=0.76) Length = 64 Score = 48.8 bits (111), Expect = 5e-06 Identities = 21/45 (46%), Positives = 31/45 (68%) Frame = +1 Query: 109 VTGVIGHRMPRYCLFGNTVNLTSRCETTGVPGTINVSEDTYNYLM 243 V GV+G + PRY +FG+TVN+ SR E+ GVP I++S + L+ Sbjct: 4 VAGVVGIKKPRYDVFGDTVNVASRMESNGVPNRIHISAECRKALL 48 >SB_10830| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1006 Score = 45.2 bits (102), Expect = 7e-05 Identities = 21/52 (40%), Positives = 32/52 (61%) Frame = +1 Query: 85 IGIHSGEVVTGVIGHRMPRYCLFGNTVNLTSRCETTGVPGTINVSEDTYNYL 240 + ++ G VV GVIG P+Y ++G+TVN+ SR +TG I V++ Y L Sbjct: 917 VSLNFGPVVAGVIGAHKPQYDIWGDTVNVASRMYSTGKDNHIQVTKAMYEIL 968 Score = 33.5 bits (73), Expect = 0.22 Identities = 13/44 (29%), Positives = 28/44 (63%) Frame = +1 Query: 73 VGITIGIHSGEVVTGVIGHRMPRYCLFGNTVNLTSRCETTGVPG 204 + + +G+H G+V +GV+G +Y ++ + V + + E+ G+PG Sbjct: 551 LNMRVGVHMGKVHSGVLGLVKWQYDVWSDDVTIANHMESGGLPG 594 >SB_6162| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1808 Score = 29.9 bits (64), Expect = 2.7 Identities = 9/19 (47%), Positives = 14/19 (73%) Frame = -1 Query: 166 LPYCRRGNNEACDDRSHLS 110 +P+C GNNE C D +H++ Sbjct: 423 VPHCSHGNNERCYDNTHMT 441 >SB_2034| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1003 Score = 29.9 bits (64), Expect = 2.7 Identities = 12/35 (34%), Positives = 23/35 (65%), Gaps = 1/35 (2%) Frame = +1 Query: 124 GHRMPRYCLFGNTVNLTSRCETTGVP-GTINVSED 225 G + P + GNT+ +T +C+ TG+P T+ +++D Sbjct: 259 GSKTPLHSFVGNTIPVTIKCQFTGLPMPTVVMTKD 293 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 22,706,922 Number of Sequences: 59808 Number of extensions: 471767 Number of successful extensions: 1052 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 945 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1048 length of database: 16,821,457 effective HSP length: 81 effective length of database: 11,977,009 effective search space used: 2359470773 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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