Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--1534
         (701 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC31G5.16c |dpm1||dolichol-phosphate mannosyltransferase catal...   114   1e-26
SPBC56F2.10c |alg5||dolichyl-phosphate beta-glucosyltransferase ...    32   0.069
SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C |Schizosacchar...    26   4.5  
SPBC12C2.07c |||spermidine synthase |Schizosaccharomyces pombe|c...    26   6.0  
SPBC646.05c |erg9||squalene synthase Erg9|Schizosaccharomyces po...    25   7.9  
SPAC29A4.15 |||serine-tRNA ligase|Schizosaccharomyces pombe|chr ...    25   7.9  
SPAC9G1.02 |wis4|wak1, wik1|MAP kinase kinase kinase Wis4|Schizo...    25   7.9  
SPAC6F12.10c |ade3|min11|phosphoribosylformylglycinamidine synth...    25   7.9  

>SPAC31G5.16c |dpm1||dolichol-phosphate mannosyltransferase
           catalytic subunit Dpm1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 236

 Score =  114 bits (275), Expect = 1e-26
 Identities = 51/80 (63%), Positives = 64/80 (80%)
 Frame = +1

Query: 31  KYSILLPTYNERENLPIIIWLIIKYLDESGVDYEVIIIDDGSPDGTSEVARQLQKLYGSS 210
           KYS+LLPTYNER+NLPII +LI K  D+  +D+E++IIDD SPDGT EVA++LQK+YG  
Sbjct: 3   KYSVLLPTYNERKNLPIITYLIAKTFDQEKLDWEIVIIDDASPDGTQEVAKELQKIYGED 62

Query: 211 KIVLRPREMKLGLGTPTFMG 270
           KI+L+PR  KLGLGT    G
Sbjct: 63  KILLKPRSGKLGLGTAYIHG 82



 Score =  104 bits (249), Expect = 1e-23
 Identities = 43/62 (69%), Positives = 54/62 (87%)
 Frame = +3

Query: 255 AYIHGIQQASGNFIIIMDADLSHHPKFIPEFIKLQLKYDYDIVSGTRYKGSGGVYGWDFK 434
           AYIHG++ A+G+F+IIMDAD SHHPK++PEFIKLQ +++YDIV GTRY   GGVYGW+ K
Sbjct: 78  AYIHGLKFATGDFVIIMDADFSHHPKYLPEFIKLQKEHNYDIVLGTRYAKDGGVYGWNLK 137

Query: 435 RK 440
           RK
Sbjct: 138 RK 139



 Score = 90.6 bits (215), Expect = 2e-19
 Identities = 42/63 (66%), Positives = 52/63 (82%)
 Frame = +1

Query: 511 FRLYKKEVLEKLILSCVSKGYVFQMEMIIRARQFDYSIGEVPISFVDRVYGESKLGGSEI 690
           FRLYKK VLE L+    SKGYVFQME+I RAR+ +Y+IGEVPI+FVDR+YGESKLG  +I
Sbjct: 164 FRLYKKPVLETLMSEVTSKGYVFQMEIIARAREHNYTIGEVPIAFVDRLYGESKLGMDDI 223

Query: 691 VQF 699
           + +
Sbjct: 224 LGY 226



 Score = 31.5 bits (68), Expect = 0.12
 Identities = 16/35 (45%), Positives = 20/35 (57%)
 Frame = +2

Query: 443 ISRGANFLTQLMLRPGVSDLTGHLDCTKRKFSRSL 547
           ISRGAN L   +L  GVSD+TG     K+    +L
Sbjct: 141 ISRGANLLASTVLGTGVSDVTGSFRLYKKPVLETL 175


>SPBC56F2.10c |alg5||dolichyl-phosphate beta-glucosyltransferase
           Alg5 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 322

 Score = 32.3 bits (70), Expect = 0.069
 Identities = 14/45 (31%), Positives = 27/45 (60%)
 Frame = +1

Query: 511 FRLYKKEVLEKLILSCVSKGYVFQMEMIIRARQFDYSIGEVPISF 645
           F+L+ +E  + +      +G++F +E++  AR F   I EVPI++
Sbjct: 236 FKLFSREAYQSIFPRMHVEGWIFDIEVLTLARFFGLPIIEVPITW 280


>SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 988

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 23/71 (32%), Positives = 32/71 (45%), Gaps = 3/71 (4%)
 Frame = +1

Query: 85  IWLIIKYLDESGVDYEVIIIDD--GSPDGTSEVARQLQKLYGSSK-IVLRPREMKLGLGT 255
           + L +KY  E+G+ Y  + +D+    PDG   +A      YG  K  +L         GT
Sbjct: 773 VCLALKYFHENGIAYRDLKLDNILLCPDGHIRIAD-----YGLCKENMLLGNTTSTFCGT 827

Query: 256 PTFMGYSKLLE 288
           P FM    LLE
Sbjct: 828 PEFMAPEILLE 838


>SPBC12C2.07c |||spermidine synthase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 298

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 20/65 (30%), Positives = 30/65 (46%), Gaps = 3/65 (4%)
 Frame = +1

Query: 1   EVDSGLIKRDKYSILLPTYNERENLPIIIWLI---IKYLDESGVDYEVIIIDDGSPDGTS 171
           ++D  +IK  K    LP  +   N P +   I    K+L +    ++VII D   PDG +
Sbjct: 119 DIDEDVIKVSKQ--YLPEMSAGFNHPKVKVHIGDGFKFLQDYQNTFDVIITDSSDPDGPA 176

Query: 172 EVARQ 186
           E   Q
Sbjct: 177 EALFQ 181


>SPBC646.05c |erg9||squalene synthase Erg9|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 460

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 20/82 (24%), Positives = 33/82 (40%)
 Frame = +1

Query: 46  LPTYNERENLPIIIWLIIKYLDESGVDYEVIIIDDGSPDGTSEVARQLQKLYGSSKIVLR 225
           LP+Y E EN+P  I    + LD +   + V+I +   P+G  E       +      V  
Sbjct: 22  LPSYGEDENVPQNIRRCYQLLDMTSRSFAVVIKE--LPNGIREAVMIFYLVLRGLDTVED 79

Query: 226 PREMKLGLGTPTFMGYSKLLEI 291
              + L    P    + K +E+
Sbjct: 80  DMTLPLDKKLPILRDFYKTIEV 101


>SPAC29A4.15 |||serine-tRNA ligase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 450

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 19/57 (33%), Positives = 27/57 (47%), Gaps = 1/57 (1%)
 Frame = +1

Query: 496 RSDWP-FRLYKKEVLEKLILSCVSKGYVFQMEMIIRARQFDYSIGEVPISFVDRVYG 663
           R  W  FR++  E +E+ +L+   K +    EMI  A  F Y   E+P   V  V G
Sbjct: 279 RDAWGIFRVHAFEKIEQFVLTDPEKSWEAFTEMINHAEDF-YKSLELPYRIVAIVSG 334


>SPAC9G1.02 |wis4|wak1, wik1|MAP kinase kinase kinase
           Wis4|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1401

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 11/32 (34%), Positives = 20/32 (62%)
 Frame = +1

Query: 580 QMEMIIRARQFDYSIGEVPISFVDRVYGESKL 675
           + ++ +R  QF+Y   E   SFV+R++ +S L
Sbjct: 393 EFDLTMRTPQFNYDGVENTSSFVERIFRQSGL 424


>SPAC6F12.10c |ade3|min11|phosphoribosylformylglycinamidine synthase
           Ade3 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1323

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 10/25 (40%), Positives = 15/25 (60%)
 Frame = +1

Query: 622 IGEVPISFVDRVYGESKLGGSEIVQ 696
           IG   + F+D   G+ +LGGS + Q
Sbjct: 840 IGSTSLIFIDLANGKQRLGGSILAQ 864


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,896,849
Number of Sequences: 5004
Number of extensions: 58267
Number of successful extensions: 152
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 152
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 325165428
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)