Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1520
(768 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g25900.1 68418.m03075 ent-kaurene oxidase, putative (GA3) / c... 38 0.010
At5g26610.2 68418.m03181 D111/G-patch domain-containing protein ... 37 0.017
At5g26610.1 68418.m03180 D111/G-patch domain-containing protein ... 37 0.017
At5g06230.2 68418.m00695 expressed protein contains Pfam profile... 29 2.6
At5g06230.1 68418.m00696 expressed protein contains Pfam profile... 29 2.6
At3g17000.1 68416.m02171 ubiquitin-conjugating enzyme, putative ... 29 2.6
At4g26030.1 68417.m03748 hypothetical protein 28 6.0
At1g25500.2 68414.m03166 choline transporter-related contains we... 28 6.0
At1g25500.1 68414.m03167 choline transporter-related contains we... 28 6.0
At4g13130.1 68417.m02045 DC1 domain-containing protein contains ... 28 7.9
At3g28970.1 68416.m03621 expressed protein contains Pfam domain ... 28 7.9
>At5g25900.1 68418.m03075 ent-kaurene oxidase, putative (GA3) /
cytochrome P450 identical to GA3 [Arabidopsis thaliana]
GI:3342249; similar to ent-kaurene oxidase [Cucurbita
maxima] GI:11934675; contains Pfam profile PF00067:
Cytochrome P450
Length = 509
Score = 37.5 bits (83), Expect = 0.010
Identities = 31/110 (28%), Positives = 54/110 (49%), Gaps = 3/110 (2%)
Frame = -3
Query: 499 ASFVITMFLKRTMPFSKRSKR*APLLRICSLPVTWNLPLIIVDRYLLQNSQKNPFLTFLM 320
+SF+ F K+ + FS+++ + + + S+PV P+I LLQ +K P TF
Sbjct: 16 SSFIFIFFFKKLLSFSRKNM--SEVSTLPSVPVVPGFPVI---GNLLQLKEKKPHKTFTR 70
Query: 319 RSSINS---ATKLLASRLFLCSSFEYAVRHLVRLEGKFRRRDSTNAMSLL 179
S I + K+ +S L + +S E A +V R +NA+++L
Sbjct: 71 WSEIYGPIYSIKMGSSSLIVLNSTETAKEAMVTRFSSISTRKLSNALTVL 120
>At5g26610.2 68418.m03181 D111/G-patch domain-containing protein
contains Pfam PF01585: G-patch domain
Length = 301
Score = 36.7 bits (81), Expect = 0.017
Identities = 15/56 (26%), Positives = 31/56 (55%)
Frame = +3
Query: 261 EEHKKSLEASSFVAEFIEDRIRKVKKGFFCEFCNKYLSTIIKGRFHVTGNEHIRNK 428
EE K E + + I+ +++++K F+CE C+K T+++ H++ +H K
Sbjct: 134 EEIAKKREVLAEREQKIQSDVKEIRKVFYCELCSKQYRTVMEFEGHLSSYDHNHKK 189
>At5g26610.1 68418.m03180 D111/G-patch domain-containing protein
contains Pfam PF01585: G-patch domain
Length = 301
Score = 36.7 bits (81), Expect = 0.017
Identities = 15/56 (26%), Positives = 31/56 (55%)
Frame = +3
Query: 261 EEHKKSLEASSFVAEFIEDRIRKVKKGFFCEFCNKYLSTIIKGRFHVTGNEHIRNK 428
EE K E + + I+ +++++K F+CE C+K T+++ H++ +H K
Sbjct: 134 EEIAKKREVLAEREQKIQSDVKEIRKVFYCELCSKQYRTVMEFEGHLSSYDHNHKK 189
>At5g06230.2 68418.m00695 expressed protein contains Pfam profile
PF03005: Arabidopsis proteins of unknown function
Length = 372
Score = 29.5 bits (63), Expect = 2.6
Identities = 22/88 (25%), Positives = 35/88 (39%)
Frame = +3
Query: 240 CRTAYSKEEHKKSLEASSFVAEFIEDRIRKVKKGFFCEFCNKYLSTIIKGRFHVTGNEHI 419
C + K + S +SS F + R + GF C + S + R+ G +
Sbjct: 21 CDYSKGKWVRRASSSSSSVNGLFYGEECRFLDSGFRCHKHGRKDSGYLDWRWQPHGCDLP 80
Query: 420 RNKGAYLFERLENGMVLFRNIVITKEAW 503
R + L ER NG ++F I + W
Sbjct: 81 RFNASDLLERSRNGRIVFVGDSIGRNQW 108
>At5g06230.1 68418.m00696 expressed protein contains Pfam profile
PF03005: Arabidopsis proteins of unknown function
Length = 413
Score = 29.5 bits (63), Expect = 2.6
Identities = 22/88 (25%), Positives = 35/88 (39%)
Frame = +3
Query: 240 CRTAYSKEEHKKSLEASSFVAEFIEDRIRKVKKGFFCEFCNKYLSTIIKGRFHVTGNEHI 419
C + K + S +SS F + R + GF C + S + R+ G +
Sbjct: 62 CDYSKGKWVRRASSSSSSVNGLFYGEECRFLDSGFRCHKHGRKDSGYLDWRWQPHGCDLP 121
Query: 420 RNKGAYLFERLENGMVLFRNIVITKEAW 503
R + L ER NG ++F I + W
Sbjct: 122 RFNASDLLERSRNGRIVFVGDSIGRNQW 149
>At3g17000.1 68416.m02171 ubiquitin-conjugating enzyme, putative
similar to Non-Canonical UBiquitin Conjugating Enzyme 1
(NCUBE1) from [Gallus gallus] GI:7362937, [Mus musculus]
GI:7363050, [Homo sapiens] GI:7362973; contains Pfam
profile PF00179: Ubiquitin-conjugating enzyme
Length = 309
Score = 29.5 bits (63), Expect = 2.6
Identities = 20/52 (38%), Positives = 25/52 (48%), Gaps = 7/52 (13%)
Frame = +2
Query: 605 DVQF--GIYGGLYRKTMD-----DSFHCLTCNEVFESPTRACISSHFLHPNH 739
D +F GIY G + D SF LT N FE+ T+ C+S HP H
Sbjct: 52 DTEFEGGIYHGRIQLPADYPFKPPSFMLLTPNGRFETNTKICLSISNYHPEH 103
>At4g26030.1 68417.m03748 hypothetical protein
Length = 220
Score = 28.3 bits (60), Expect = 6.0
Identities = 9/31 (29%), Positives = 17/31 (54%)
Frame = +2
Query: 650 DDSFHCLTCNEVFESPTRACISSHFLHPNHQ 742
D SF CL CN +F++ + + +H ++
Sbjct: 155 DSSFICLKCNSLFDTSQMLVVHTELIHSKNE 185
>At1g25500.2 68414.m03166 choline transporter-related contains weak
similarity to CD92 protein [Homo sapiens]
gi|16945323|emb|CAC82175
Length = 488
Score = 28.3 bits (60), Expect = 6.0
Identities = 13/51 (25%), Positives = 28/51 (54%)
Frame = +2
Query: 509 IIGKKCIICAIEFNDVKKHITSVKHIFNMLKFDVQFGIYGGLYRKTMDDSF 661
I G +C++C ++F + + ++ FN + VQ +YG + K+ D++
Sbjct: 320 ICGNECLLCCVDF--LFHLVETLVRFFNKYAY-VQIAVYGKGFNKSARDAW 367
>At1g25500.1 68414.m03167 choline transporter-related contains weak
similarity to CD92 protein [Homo sapiens]
gi|16945323|emb|CAC82175
Length = 432
Score = 28.3 bits (60), Expect = 6.0
Identities = 13/51 (25%), Positives = 28/51 (54%)
Frame = +2
Query: 509 IIGKKCIICAIEFNDVKKHITSVKHIFNMLKFDVQFGIYGGLYRKTMDDSF 661
I G +C++C ++F + + ++ FN + VQ +YG + K+ D++
Sbjct: 320 ICGNECLLCCVDF--LFHLVETLVRFFNKYAY-VQIAVYGKGFNKSARDAW 367
>At4g13130.1 68417.m02045 DC1 domain-containing protein contains
Pfam protein PF03107 DC1 domain
Length = 767
Score = 27.9 bits (59), Expect = 7.9
Identities = 14/38 (36%), Positives = 19/38 (50%), Gaps = 2/38 (5%)
Frame = +2
Query: 632 LYRKTMDDSFHCLTCN-EVFESPTRACIS-SHFLHPNH 739
+++ T FHC+ CN E E + I H LHP H
Sbjct: 223 IWKSTRQIHFHCILCNGENHEEYDKVPIEVKHPLHPRH 260
>At3g28970.1 68416.m03621 expressed protein contains Pfam domain
PF03556: Domain of unknown function (DUF298)
Length = 295
Score = 27.9 bits (59), Expect = 7.9
Identities = 16/60 (26%), Positives = 27/60 (45%)
Frame = +3
Query: 105 NRDSLCFICCAYLKDGFEVYQERNTNKDIAFVLSRLRNLPSKRTRCRTAYSKEEHKKSLE 284
N+D+ F C + +YQE +KD + LRN+P + + +EE + E
Sbjct: 186 NKDTSLFCKCGDTESESCLYQEDEHHKDYRRPHTGLRNIPGLKRKTSKKNDEEEEDEDEE 245
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,148,012
Number of Sequences: 28952
Number of extensions: 333917
Number of successful extensions: 1012
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 985
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1012
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1721869952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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