BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--1510 (840 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g66680.1 68418.m08406 dolichyl-diphosphooligosaccharide-prote... 79 5e-15 At2g01340.1 68415.m00051 expressed protein 29 5.1 At3g06500.1 68416.m00754 beta-fructofuranosidase, putative / inv... 28 6.7 >At5g66680.1 68418.m08406 dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48kDa subunit family protein similar to SP|Q05052 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit precursor (EC 2.4.1.119) (Oligosaccharyl transferase 48 kDa subunit) {Canis familiaris}; contains Pfam profile PF03345: Dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48kD subunit Length = 437 Score = 78.6 bits (185), Expect = 5e-15 Identities = 33/60 (55%), Positives = 46/60 (76%) Frame = +2 Query: 68 DHETLVLIDNLNIKETHSQFFKSLQERGYGLTFKLADDANLVLSKYGEYLYKNLIVFAPS 247 D LVL+D+L++K +HS FF +L+ RG+ L FKLA+D+ L L +YG+YLY LI+FAPS Sbjct: 30 DRRVLVLLDDLSLKSSHSIFFNTLKSRGFDLDFKLAEDSKLALQRYGQYLYDGLIIFAPS 89 Score = 75.8 bits (178), Expect = 3e-14 Identities = 37/82 (45%), Positives = 50/82 (60%) Frame = +3 Query: 516 PLLFEGTGLIVDKDNSLVLPILSADSTAYSYNPKSQVKEYPHAVGRKTVLIAALQARNNA 695 P+LF G ++ N+LVL +LSA +AYS NP S++ P G L++ +QARNNA Sbjct: 181 PVLFRGVAHSLNPTNNLVLKVLSASPSAYSANPSSKLSSPPQLTGSSISLVSVMQARNNA 240 Query: 696 RIVFSGSLFFFSDEAFNSPVTK 761 R+V SGS+ FSD S V K Sbjct: 241 RVVISGSVQLFSDRLIRSGVQK 262 Score = 67.3 bits (157), Expect = 1e-11 Identities = 34/86 (39%), Positives = 55/86 (63%), Gaps = 2/86 (2%) Frame = +1 Query: 253 RFGGQVDSEAITKFIDDXXXXXXXXXXXXXDVYREIASECGFEMDEESAA-VIDHFNYDV 429 RFGG +DS++I F+D D+ R IA+ECG + DE+S+A VIDH ++ V Sbjct: 92 RFGGSLDSKSIADFVDSGRDLILSADTAASDLIRGIATECGVDFDEDSSAMVIDHTSFSV 151 Query: 430 TD-EGDHTRIVVSPKNLIKAPTIVGE 504 +D +GDHT +++ +L+K+ I+G+ Sbjct: 152 SDVDGDHT--LIAADDLVKSDVILGK 175 >At2g01340.1 68415.m00051 expressed protein Length = 215 Score = 28.7 bits (61), Expect = 5.1 Identities = 22/78 (28%), Positives = 32/78 (41%), Gaps = 4/78 (5%) Frame = -2 Query: 467 GDTTMRVWSPSSVTS*LKWSMTAADSSSISNPH----SEAISLYTSPAAALPAIRRFPPS 300 G T +V T LK +TA + H SE++ Y + A L A +R Sbjct: 7 GKKTTKVMKIDGETFKLKTPVTAEEVLKDFPGHVLLDSESVKHYGARAKPLEAKQRLEAK 66 Query: 299 SMNLVMASLSTCPPNRAR 246 + V+ + CPP R R Sbjct: 67 RLYFVVEPVKECPPRRVR 84 >At3g06500.1 68416.m00754 beta-fructofuranosidase, putative / invertase, putative / saccharase, putative / beta-fructosidase, putative similar to neutral invertase [Daucus carota] GI:4200165; contains Pfam profile PF04853: Plant neutral invertase Length = 664 Score = 28.3 bits (60), Expect = 6.7 Identities = 15/39 (38%), Positives = 21/39 (53%) Frame = -1 Query: 432 SHIIVEVVYDCCRFLVHLKSTF*SDFSIYVSGRGVTSHK 316 S I V + CCRFL+ +S+ FS SG+ + S K Sbjct: 5 SCICVSAMKPCCRFLISFRSSSLFGFSPPNSGKFINSSK 43 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 17,701,471 Number of Sequences: 28952 Number of extensions: 369349 Number of successful extensions: 1015 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 985 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1015 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1941125600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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