Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1502
(716 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomy... 50 4e-07
SPAC20G8.02 |||phospholipase|Schizosaccharomyces pombe|chr 1|||M... 29 0.88
SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomy... 29 0.88
SPBC405.03c |||membrane transporter |Schizosaccharomyces pombe|c... 27 3.5
SPAC16A10.04 |rho4||Rho family GTPase Rho4|Schizosaccharomyces p... 27 3.5
SPBC776.06c |||spindle pole body interacting protein |Schizosacc... 27 3.5
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 26 4.7
SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb... 26 6.2
SPCC330.05c |ura4||orotidine 5'-phosphate decarboxylase Ura4 |Sc... 26 6.2
SPCC4E9.01c |rec11|SPCC550.16c|meiotic cohesin complex subunit R... 26 6.2
SPCC1620.13 |||phosphoglycerate mutase family|Schizosaccharomyce... 26 6.2
SPBC609.04 |caf5||spermine family transmembrane transporter Caf5... 25 8.2
SPBC56F2.07c |||AAA family ATPase, unknown biological role|Schiz... 25 8.2
>SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 754
Score = 49.6 bits (113), Expect = 4e-07
Identities = 19/41 (46%), Positives = 27/41 (65%)
Frame = +2
Query: 131 WLMPNGNPTGIYVYNCIANQRVPIILNDPHIATWYSCGPTV 253
W +P G T +YVYN + + +VP + N ++ TWY CGPTV
Sbjct: 11 WGVPKGQRTELYVYNTLTHSKVPFVSNGSNL-TWYCCGPTV 50
Score = 44.8 bits (101), Expect = 1e-05
Identities = 32/83 (38%), Positives = 41/83 (49%), Gaps = 14/83 (16%)
Frame = +3
Query: 510 INSIEKFVQRLIDNDMAYVSKNGSVYFDTDKFSN----YGKLQ-------KMQDSGEPSD 656
+ I FVQR+ID AY +GSVYFDT+ F Y KL+ ++ GE S
Sbjct: 251 VPEIVDFVQRIIDRGYAYPVTDGSVYFDTEAFEKGGHFYAKLEPWNKGNRELIAEGEGSL 310
Query: 657 A---DKRNKMAFALWKVTSPRNP 716
A K+ FALWK + P P
Sbjct: 311 AALTGKKRPGDFALWKASKPGEP 333
Score = 38.7 bits (86), Expect = 8e-04
Identities = 16/40 (40%), Positives = 25/40 (62%)
Frame = +1
Query: 244 PHGIESAHIGHASCYVKLDIIQRILKSFFNIKIVSAMGIT 363
P +++H+GHA YV DI++RIL+S+F I +T
Sbjct: 48 PTVYDASHMGHARNYVTTDILRRILQSYFGYNITFVQNVT 87
Score = 34.7 bits (76), Expect = 0.013
Identities = 16/32 (50%), Positives = 21/32 (65%)
Frame = +1
Query: 412 FTNIAKQYEHEFWLDMASLNVAKPLIITRVSE 507
F +A +E +F DMA+LNV P +TRVSE
Sbjct: 218 FRKLAAYWEDDFNKDMANLNVLPPTAVTRVSE 249
>SPAC20G8.02 |||phospholipase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 757
Score = 28.7 bits (61), Expect = 0.88
Identities = 28/83 (33%), Positives = 36/83 (43%), Gaps = 10/83 (12%)
Frame = +2
Query: 143 NGNPTGIYVYNCIANQRVPIILNDPHI----ATWY-SCG----PTVSNLLT*VTPAAM*N 295
N N +Y N ++ + PI + P TW+ S G P NL T V + N
Sbjct: 151 NVNEDYLYSVNVVSRELSPIYWDGPVYRILRGTWFFSRGDKLYPCEENLATQVEEGYL-N 209
Query: 296 SISYREFSN-RSSI*KSSPLWVL 361
S YREFSN + S S W L
Sbjct: 210 SCPYREFSNEKDSAAAQSKTWAL 232
>SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 874
Score = 28.7 bits (61), Expect = 0.88
Identities = 13/45 (28%), Positives = 23/45 (51%)
Frame = +1
Query: 223 RYLVFLWPHGIESAHIGHASCYVKLDIIQRILKSFFNIKIVSAMG 357
+Y++ ++P+ HIGH Y DI+ R + K++ MG
Sbjct: 60 KYILSMFPYPSGLLHIGHVRVYTISDILSRYYR-MKGYKVIHPMG 103
>SPBC405.03c |||membrane transporter |Schizosaccharomyces pombe|chr
2|||Manual
Length = 341
Score = 26.6 bits (56), Expect = 3.5
Identities = 14/24 (58%), Positives = 15/24 (62%)
Frame = -2
Query: 583 TEPFLLT*AMSLSISLCTNFSILL 512
T P L+T MSLSI L F ILL
Sbjct: 287 TSPLLVTVGMSLSIPLALFFDILL 310
>SPAC16A10.04 |rho4||Rho family GTPase Rho4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 203
Score = 26.6 bits (56), Expect = 3.5
Identities = 12/35 (34%), Positives = 20/35 (57%)
Frame = -3
Query: 336 YIEERFENSLYDIEFHIAAGVTYVSRFDTVGPQEY 232
Y+ FEN + DI + + V ++ +DT G +EY
Sbjct: 43 YVPTVFENYITDITYGPNSKVIELALWDTAGQEEY 77
>SPBC776.06c |||spindle pole body interacting protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 598
Score = 26.6 bits (56), Expect = 3.5
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +2
Query: 62 VFNLTFVRSLSHVCSLKPYKDTKWLMPNGNPT 157
VF + + HV +LKP DT W + + +P+
Sbjct: 104 VFAMAICTTFPHVHALKPLLDTAWEIFDSSPS 135
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 26.2 bits (55), Expect = 4.7
Identities = 9/40 (22%), Positives = 20/40 (50%)
Frame = -1
Query: 410 CVLLSRPFLMILSSISVIPIAETIFILKNDLRILCMISSF 291
C +P+ + L ++P++ + I+ D R LC + +
Sbjct: 1255 CESADKPYALPLLDSGMVPVSTQLAIVNPDTRELCRVGEY 1294
>SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 2685
Score = 25.8 bits (54), Expect = 6.2
Identities = 26/91 (28%), Positives = 46/91 (50%), Gaps = 11/91 (12%)
Frame = +1
Query: 436 EHEFWLDMASLNVAKPLIITRVSEPLIVLRNSYRGL------LITTWLM*VKTVQY---I 588
E + LD A N ++IT P +VLR SYRG IT ++ V T++Y +
Sbjct: 2456 ELQSMLDRAGSN----MLITYFKIPSVVLRLSYRGTGGLGLENITGFVFTVPTMEYRNEV 2511
Query: 589 LIPINSQTMANYKKCKTVVNH--QMLIKEIK 675
++ + +TVV+H ++L++++K
Sbjct: 2512 CSYLDLAMKLKHDLIRTVVSHTGKLLVEKVK 2542
>SPCC330.05c |ura4||orotidine 5'-phosphate decarboxylase Ura4
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 264
Score = 25.8 bits (54), Expect = 6.2
Identities = 12/42 (28%), Positives = 24/42 (57%)
Frame = +3
Query: 510 INSIEKFVQRLIDNDMAYVSKNGSVYFDTDKFSNYGKLQKMQ 635
I+ +E F Q +++ +A K+ + F+ KF++ G K+Q
Sbjct: 65 IDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVKLQ 106
>SPCC4E9.01c |rec11|SPCC550.16c|meiotic cohesin complex subunit
Rec11|Schizosaccharomyces pombe|chr 3|||Manual
Length = 923
Score = 25.8 bits (54), Expect = 6.2
Identities = 11/36 (30%), Positives = 20/36 (55%)
Frame = -3
Query: 399 FEAFLNDFVVYIRNTHSGDDFYIEERFENSLYDIEF 292
FE++ D +I + DD ++ + EN+ YD +F
Sbjct: 471 FESWFRDSSDHIFSRVKDDDKFVYQSQENNFYDADF 506
>SPCC1620.13 |||phosphoglycerate mutase family|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 282
Score = 25.8 bits (54), Expect = 6.2
Identities = 15/46 (32%), Positives = 23/46 (50%), Gaps = 1/46 (2%)
Frame = -1
Query: 554 VVINKPLYEFLNTINGSDTLVMMSGFATFNDAISSQNSCSY-CLAI 420
+V +P E NTI D ++ + F T + SSQN + CL +
Sbjct: 14 IVEEEPQQEITNTILEEDFNLLDNSFQTDISSTSSQNDSKFTCLLV 59
>SPBC609.04 |caf5||spermine family transmembrane transporter
Caf5|Schizosaccharomyces pombe|chr 2|||Manual
Length = 531
Score = 25.4 bits (53), Expect = 8.2
Identities = 10/30 (33%), Positives = 21/30 (70%)
Frame = +1
Query: 457 MASLNVAKPLIITRVSEPLIVLRNSYRGLL 546
+A + +P+I++ +SEP+++ N Y GL+
Sbjct: 302 LAKETIIRPIILS-ISEPIVLSLNIYIGLI 330
>SPBC56F2.07c |||AAA family ATPase, unknown biological
role|Schizosaccharomyces pombe|chr 2|||Manual
Length = 809
Score = 25.4 bits (53), Expect = 8.2
Identities = 12/40 (30%), Positives = 21/40 (52%)
Frame = -2
Query: 646 SPLSCIFCNLP*FENLSVSKYTEPFLLT*AMSLSISLCTN 527
SPL + NL F+ +++S+Y P + + SL +N
Sbjct: 71 SPLLAKWANLKAFQRVNISQYVHPLKEAEGIKIVASLSSN 110
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,100,268
Number of Sequences: 5004
Number of extensions: 68139
Number of successful extensions: 192
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 183
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 190
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 335201398
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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