Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--1476
         (770 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac...    72   1e-13
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc...    32   0.079
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha...    27   2.2  
SPCC290.02 |rpc34||DNA-directed RNA polymerase III complex subun...    27   2.2  
SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr ...    27   3.0  
SPAC167.05 ||SPAC57A7.01|Usp |Schizosaccharomyces pombe|chr 1|||...    26   5.2  
SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor |S...    26   6.9  

>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
           Txl1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 290

 Score = 71.7 bits (168), Expect = 1e-13
 Identities = 38/88 (43%), Positives = 54/88 (61%), Gaps = 5/88 (5%)
 Frame = +1

Query: 517 DEQLIINISFNQLVKLHSIKI---KGPADKGPKSIKLFINQPRTLDFDQAAGYSSVQDLE 687
           DEQL+I I F ++VK+HSI I   KG     PK+IKL+INQP  L F+ A  ++  Q +E
Sbjct: 166 DEQLMIYIPFLEVVKVHSIAITPVKGETSSAPKTIKLYINQPNNLSFEDAESFTPTQVIE 225

Query: 688 LTPSDLEGNP--VPLKFVKFQSVQTFTL 765
               + +  P  +PL+FVKFQ V +  +
Sbjct: 226 DIVYEQDDQPTIIPLRFVKFQRVNSLVI 253



 Score = 54.0 bits (124), Expect = 2e-08
 Identities = 30/86 (34%), Positives = 41/86 (47%), Gaps = 4/86 (4%)
 Frame = +2

Query: 248 GRRAMPTFIFYRNRTKIDRLEGGNTTVLENKV----RQYYGSDVAEEDEDNTVAGHMDLI 415
           G +AMPTF+F+ N  +ID L G N   L+ KV     +  G+          V G   L 
Sbjct: 71  GVKAMPTFVFFENGKQIDMLTGANPQALKEKVALISSKATGTGALASSSSAPVKGFASLQ 130

Query: 416 TFITKSECECLNEADDHPLAQALTND 493
             I   + ECLN+ DDH L  A  ++
Sbjct: 131 GCIENPQLECLNQQDDHDLKSAFNSN 156


>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 103

 Score = 32.3 bits (70), Expect = 0.079
 Identities = 13/33 (39%), Positives = 19/33 (57%)
 Frame = +2

Query: 248 GRRAMPTFIFYRNRTKIDRLEGGNTTVLENKVR 346
           G  AMP+F  Y+N  KI+ + G N   LE  ++
Sbjct: 68  GVHAMPSFFLYKNGEKIEEIVGANPAKLEASIK 100



 Score = 29.9 bits (64), Expect = 0.42
 Identities = 14/34 (41%), Positives = 20/34 (58%)
 Frame = +3

Query: 195 AVFLKVDVDRCADTASAQGVVQCPHSFSTETGPK 296
           A F+KVDVD+ ++ A+  GV   P  F  + G K
Sbjct: 50  ATFIKVDVDQLSEIAAEAGVHAMPSFFLYKNGEK 83


>SPBC12D12.07c |trx2||mitochondrial thioredoxin
           Trx2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 121

 Score = 27.5 bits (58), Expect = 2.2
 Identities = 10/35 (28%), Positives = 20/35 (57%)
 Frame = +2

Query: 248 GRRAMPTFIFYRNRTKIDRLEGGNTTVLENKVRQY 352
           G  A+PT + +R   ++DR+ G +   L + + +Y
Sbjct: 85  GVYALPTMVLFRKGQELDRIVGADVKTLSSLLAKY 119


>SPCC290.02 |rpc34||DNA-directed RNA polymerase III complex subunit
           Rpc34|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 301

 Score = 27.5 bits (58), Expect = 2.2
 Identities = 9/23 (39%), Positives = 17/23 (73%)
 Frame = -2

Query: 532 LSIVHHSNSKVSPVICQCLGKWM 464
           +S +  +NS+V+P+ C+ L KW+
Sbjct: 278 VSDICDANSRVNPITCEYLDKWL 300


>SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1337

 Score = 27.1 bits (57), Expect = 3.0
 Identities = 13/26 (50%), Positives = 15/26 (57%)
 Frame = +2

Query: 392  VAGHMDLITFITKSECECLNEADDHP 469
            V  H+D I FITKS C  + E D  P
Sbjct: 1285 VVNHIDRIMFITKSLCGEIFEIDKWP 1310


>SPAC167.05 ||SPAC57A7.01|Usp |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 601

 Score = 26.2 bits (55), Expect = 5.2
 Identities = 10/30 (33%), Positives = 18/30 (60%)
 Frame = +2

Query: 317 NTTVLENKVRQYYGSDVAEEDEDNTVAGHM 406
           NT+ + + +  +YGSD+ E ++D   A  M
Sbjct: 366 NTSFVSSNINNWYGSDLEELEQDVKTAKSM 395


>SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1052

 Score = 25.8 bits (54), Expect = 6.9
 Identities = 12/39 (30%), Positives = 17/39 (43%)
 Frame = +1

Query: 502 PCCYCDEQLIINISFNQLVKLHSIKIKGPADKGPKSIKL 618
           P  +C + L+  +      +LH  K KGP D      KL
Sbjct: 546 PMIWCSQFLLAGVVCESNFELHLYKAKGPLDDRENLAKL 584


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,852,030
Number of Sequences: 5004
Number of extensions: 52532
Number of successful extensions: 141
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 138
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 371330890
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)