Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--1461
         (760 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig...    76   6e-15
SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||...    43   5e-05
SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa...    40   3e-04
SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomy...    36   0.005
SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyc...    36   0.008
SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo...    34   0.025
SPBP8B7.27 |mug30||ubiquitin-protein ligase E3|Schizosaccharomyc...    33   0.033
SPAC1A6.05c |||triacylglycerol lipase|Schizosaccharomyces pombe|...    27   3.8  

>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
            E3 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1647

 Score = 75.8 bits (178), Expect = 6e-15
 Identities = 34/63 (53%), Positives = 45/63 (71%)
 Frame = +2

Query: 74   DHGYNAESRGIRMLIDILASYNREEQRHFLQFVTGSPRLPTGGFKALNPPLTVVRKSLES 253
            DHGY  ES  I+ L+ +++  N +EQR FLQF+TGS +LP GGF  LNPPLTVVR+  E 
Sbjct: 1541 DHGYTMESPTIQRLLTLMSQMNFQEQRDFLQFITGSRKLPIGGFAGLNPPLTVVRRLNEP 1600

Query: 254  YWI 262
             ++
Sbjct: 1601 PYV 1603



 Score = 72.1 bits (169), Expect = 8e-14
 Identities = 32/44 (72%), Positives = 38/44 (86%)
 Frame = +1

Query: 262  PDEYLPSVMTCVNYLKLPDYSSAEVMRAKLRLAASEGQHSFHLS 393
            PD+YLPSVMTCVNYLKLP+YSS+EV+ ++L  A  EGQ SFHLS
Sbjct: 1604 PDDYLPSVMTCVNYLKLPEYSSSEVLGSRLSKAILEGQGSFHLS 1647


>SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 3227

 Score = 42.7 bits (96), Expect = 5e-05
 Identities = 18/46 (39%), Positives = 27/46 (58%)
 Frame = +2

Query: 77   HGYNAESRGIRMLIDILASYNREEQRHFLQFVTGSPRLPTGGFKAL 214
            HGYN  S  ++     + S++ EE+   LQF TG+ ++P  GFK L
Sbjct: 3122 HGYNVSSPQVQWFWRAVRSFDEEERAKLLQFATGTSKVPLNGFKEL 3167



 Score = 35.9 bits (79), Expect = 0.006
 Identities = 16/37 (43%), Positives = 21/37 (56%)
 Frame = +1

Query: 274  LPSVMTCVNYLKLPDYSSAEVMRAKLRLAASEGQHSF 384
            LP   TC N L LP+Y + E +R+ L  A +EG   F
Sbjct: 3188 LPQSHTCFNQLDLPEYDTYEQLRSMLLTAINEGSEGF 3224


>SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1029

 Score = 40.3 bits (90), Expect = 3e-04
 Identities = 19/51 (37%), Positives = 28/51 (54%)
 Frame = +2

Query: 80   GYNAESRGIRMLIDILASYNREEQRHFLQFVTGSPRLPTGGFKALNPPLTV 232
            GY   S  I +  ++L  +  E++R F++FVT   R P  GFKAL P   +
Sbjct: 931  GYEPNSPTIVLFWEVLREFEEEDKRSFVKFVTSVARPPILGFKALMPSFCI 981



 Score = 37.9 bits (84), Expect = 0.002
 Identities = 17/33 (51%), Positives = 21/33 (63%)
 Frame = +1

Query: 274  LPSVMTCVNYLKLPDYSSAEVMRAKLRLAASEG 372
            LP+  TCVN LKLP YS+ + +R KL  A   G
Sbjct: 991  LPTASTCVNLLKLPMYSTKQTLRDKLLTAVRSG 1023


>SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 786

 Score = 36.3 bits (80), Expect = 0.005
 Identities = 16/44 (36%), Positives = 25/44 (56%)
 Frame = +2

Query: 83  YNAESRGIRMLIDILASYNREEQRHFLQFVTGSPRLPTGGFKAL 214
           Y A    I+   +I+A +  E++   LQF TG+ R+P  GF+ L
Sbjct: 685 YIATDPVIKWFWEIIAGWKNEDRSKLLQFATGTSRIPVNGFRDL 728



 Score = 34.7 bits (76), Expect = 0.014
 Identities = 17/33 (51%), Positives = 20/33 (60%)
 Frame = +1

Query: 262 PDEYLPSVMTCVNYLKLPDYSSAEVMRAKLRLA 360
           PD+ LP   TC N L LPDY S + +  KL LA
Sbjct: 745 PDQ-LPVAHTCFNRLDLPDYPSKDTLHEKLSLA 776


>SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 767

 Score = 35.5 bits (78), Expect = 0.008
 Identities = 14/44 (31%), Positives = 26/44 (59%)
 Frame = +2

Query: 83  YNAESRGIRMLIDILASYNREEQRHFLQFVTGSPRLPTGGFKAL 214
           Y+   + I+   +++  ++ E++   LQF TG+ R+P  GFK L
Sbjct: 666 YSENDQIIKWFWELMDEWSNEKKSRLLQFTTGTSRIPVNGFKDL 709



 Score = 30.3 bits (65), Expect = 0.31
 Identities = 14/32 (43%), Positives = 18/32 (56%)
 Frame = +1

Query: 274 LPSVMTCVNYLKLPDYSSAEVMRAKLRLAASE 369
           LP   TC N L LP Y+S + +  KL +A  E
Sbjct: 729 LPKAHTCFNRLDLPPYTSKKDLDHKLSIAVEE 760


>SPAC1805.15c |pub2||ubiquitin-protein ligase
           Pub2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 671

 Score = 33.9 bits (74), Expect = 0.025
 Identities = 13/33 (39%), Positives = 23/33 (69%)
 Frame = +2

Query: 119 DILASYNREEQRHFLQFVTGSPRLPTGGFKALN 217
           ++L+ ++ E++   LQF TG+ RLP  GFK ++
Sbjct: 582 ELLSEWSPEKKAKLLQFATGTSRLPLSGFKDMH 614



 Score = 32.3 bits (70), Expect = 0.077
 Identities = 14/32 (43%), Positives = 18/32 (56%)
 Frame = +1

Query: 274 LPSVMTCVNYLKLPDYSSAEVMRAKLRLAASE 369
           LP   TC N L +P Y+S E +  KL +A  E
Sbjct: 633 LPKAHTCFNRLDIPPYNSKEELEQKLTIAIQE 664


>SPBP8B7.27 |mug30||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 807

 Score = 33.5 bits (73), Expect = 0.033
 Identities = 15/42 (35%), Positives = 26/42 (61%)
 Frame = +2

Query: 77  HGYNAESRGIRMLIDILASYNREEQRHFLQFVTGSPRLPTGG 202
           H + ++ + I    D+++ Y+ + Q+ FL FVTGS R+P  G
Sbjct: 710 HSFVSKRKIILWFWDLISHYSLKMQKLFLIFVTGSDRIPATG 751



 Score = 30.7 bits (66), Expect = 0.24
 Identities = 17/40 (42%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
 Frame = +1

Query: 253 LLDPD-EYLPSVMTCVNYLKLPDYSSAEVMRAKLRLAASE 369
           +L PD + LP   TC N+L + +YSS E ++ KL  A  E
Sbjct: 761 VLGPDSDQLPISHTCFNHLCIWEYSSREKLKKKLDTALLE 800


>SPAC1A6.05c |||triacylglycerol lipase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 483

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 15/44 (34%), Positives = 23/44 (52%), Gaps = 1/44 (2%)
 Frame = +1

Query: 265 DEYLPSVMTCVNYL-KLPDYSSAEVMRAKLRLAASEGQHSFHLS 393
           DE++  V+ C+ YL + PD S  E +    RL  + G  +  LS
Sbjct: 101 DEFIQEVLMCLTYLEETPDLSLDEKITEFSRLKLTTGNTALILS 144


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,315,679
Number of Sequences: 5004
Number of extensions: 69208
Number of successful extensions: 156
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 156
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 363302114
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)