Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--1459
         (736 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC13E7.02 |cwf24||GCN5-related N acetyltransferase|Schizosacch...    63   3e-11
SPCC663.13c |||N-acetyltransferase |Schizosaccharomyces pombe|ch...    51   1e-07
SPBC15D4.06 |||NatC N-acetyltransferase complex catalytic subuni...    39   8e-04
SPCC16C4.12 |||NatB N-acetyltransferase complex catalytic subuni...    33   0.042
SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo...    26   6.4  
SPAC17H9.04c |||RNA-binding protein|Schizosaccharomyces pombe|ch...    26   6.4  
SPAC105.01c |||potassium ion/proton antiporter|Schizosaccharomyc...    25   8.5  
SPAC11E3.11c |||guanyl-nucleotide exchange factor |Schizosacchar...    25   8.5  

>SPBC13E7.02 |cwf24||GCN5-related N
           acetyltransferase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 533

 Score = 63.3 bits (147), Expect = 3e-11
 Identities = 33/76 (43%), Positives = 43/76 (56%)
 Frame = +3

Query: 282 LYIMTLGCLYPYRRLGIGSMMVKHVLNYVKQDGNFDSIFLHVQVNNEGAIDSYKXFGFEI 461
           LY+  L  L PYR LGIGS+++ HV      + N D I LHVQ  NE  I  Y   GF+I
Sbjct: 442 LYVTVLCVLAPYRCLGIGSLLIDHVKK-TAINNNIDRISLHVQTTNESVIKWYTAHGFKI 500

Query: 462 VETKERYYKRIEPADA 509
           V+    +Y+R+E   A
Sbjct: 501 VKQINDFYRRLENKSA 516



 Score = 46.0 bits (104), Expect = 6e-06
 Identities = 22/56 (39%), Positives = 35/56 (62%)
 Frame = +1

Query: 79  AKIELGDVTPHNIKQLKKLNTVVFPVSYNDKFYKDVLEAGELAKLAYYNDIVVGAV 246
           +K  + ++T  NI   K+L  VV   SY+DKFY+ VL+  + A++A + D  VGA+
Sbjct: 377 SKYFIREITESNIVHFKRLVRVVLEASYSDKFYRLVLKNPDYARIATFEDKFVGAI 432


>SPCC663.13c |||N-acetyltransferase |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 144

 Score = 51.2 bits (117), Expect = 1e-07
 Identities = 26/67 (38%), Positives = 40/67 (59%), Gaps = 4/67 (5%)
 Frame = +1

Query: 85  IELGDVTPHNIKQLKKLNTVVFP---VSYNDKFYKDVLEAGELAKLAYYNDIVVGAV-CC 252
           IEL  + P+N+K L+ +N   F    + +   FYKD +  G LA+ AY+N + VGAV C 
Sbjct: 2   IELDAINPNNLKILEVINEKCFDPEIIIFPTSFYKDTISVGPLAQYAYFNQVCVGAVRCK 61

Query: 253 R*THRRT 273
           + TH ++
Sbjct: 62  KETHNKS 68


>SPBC15D4.06 |||NatC N-acetyltransferase complex catalytic subunit
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 150

 Score = 38.7 bits (86), Expect = 8e-04
 Identities = 26/80 (32%), Positives = 38/80 (47%)
 Frame = +3

Query: 279 RLYIMTLGCLYPYRRLGIGSMMVKHVLNYVKQDGNFDSIFLHVQVNNEGAIDSYKXFGFE 458
           R YI  L  +  YR  GI + + +  L+ +K  G    I L  +V+NE A+  Y+  GF 
Sbjct: 69  RGYIAMLAIVKEYRGQGIATKLTQASLDVMKNRGA-QEIVLETEVDNEAAMSFYERLGFC 127

Query: 459 IVETKERYYKRIEPADAMSY 518
             +   RYY  +   DA  Y
Sbjct: 128 RYKRLYRYY--LNGTDAFRY 145


>SPCC16C4.12 |||NatB N-acetyltransferase complex catalytic subunit
           Nat3 |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 180

 Score = 33.1 bits (72), Expect = 0.042
 Identities = 22/70 (31%), Positives = 34/70 (48%), Gaps = 3/70 (4%)
 Frame = +3

Query: 318 RRLGIGSMMVKHVLNYVKQDGNFDSIF---LHVQVNNEGAIDSYKXFGFEIVETKERYYK 488
           RRLG+   M    ++Y++  GN ++ F   L V+ +N  AID YK  G+ +      YY 
Sbjct: 84  RRLGLARTM----MDYLETVGNSENAFFVDLFVRASNALAIDFYKGLGYSVYRRVIGYYS 139

Query: 489 RIEPADAMSY 518
                D  S+
Sbjct: 140 NPHGKDEDSF 149


>SPAC1805.15c |pub2||ubiquitin-protein ligase
           Pub2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 671

 Score = 25.8 bits (54), Expect = 6.4
 Identities = 11/41 (26%), Positives = 25/41 (60%)
 Frame = -3

Query: 461 NFKAEXLVRVYCTFIIHLYV*KNTVKISILFHIIEHMLNHH 339
           N   + L  V+  F+ +    K+++KI++ + +I+H+L H+
Sbjct: 188 NLTPKQLKEVFSQFLFNNQS-KSSLKINLEYKVIKHLLEHY 227


>SPAC17H9.04c |||RNA-binding protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 604

 Score = 25.8 bits (54), Expect = 6.4
 Identities = 12/31 (38%), Positives = 17/31 (54%)
 Frame = +3

Query: 306 LYPYRRLGIGSMMVKHVLNYVKQDGNFDSIF 398
           +YPY +L I S+   H  N+  Q+G   S F
Sbjct: 513 VYPYSQLSINSVTANHGQNFGGQNGGNVSRF 543


>SPAC105.01c |||potassium ion/proton antiporter|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 898

 Score = 25.4 bits (53), Expect = 8.5
 Identities = 12/40 (30%), Positives = 22/40 (55%)
 Frame = -2

Query: 510 WRLLVLFSYSNVLLFQQFQSRXSCKSLLHLHYSLVRVKKY 391
           W L V    SN+L   Q  ++   ++L+ L  + V++KK+
Sbjct: 498 WTLFVDSRTSNLLRASQVDNKVENEALMGLFETFVKIKKF 537


>SPAC11E3.11c |||guanyl-nucleotide exchange factor
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 942

 Score = 25.4 bits (53), Expect = 8.5
 Identities = 7/14 (50%), Positives = 13/14 (92%)
 Frame = -1

Query: 331 IPSLLYGYKHPSVI 290
           +PS++Y +KHP+V+
Sbjct: 661 LPSVIYNHKHPNVV 674


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,951,331
Number of Sequences: 5004
Number of extensions: 62515
Number of successful extensions: 142
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 135
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 140
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 347244562
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)