BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--1453 (820 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC10F6.16 |mug134||endosulphine family protein|Schizosaccharom... 31 0.20 SPAC13G7.10 |mug152||transcription factor |Schizosaccharomyces p... 28 1.8 SPAC4A8.11c |fas2|lsd1|fatty acid synthase alpha subunit Lsd1 |S... 26 5.6 SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 9.8 SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces p... 25 9.8 SPAC13G7.04c |mac1||membrane anchored protein Mac1 |Schizosaccha... 25 9.8 >SPAC10F6.16 |mug134||endosulphine family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 139 Score = 31.1 bits (67), Expect = 0.20 Identities = 22/72 (30%), Positives = 34/72 (47%) Frame = +3 Query: 207 QGEKYRKWRVDCSCSRAGLASVLAICALGATWRLCCRKRSPDEESLDY*HAARGAPNAPP 386 QG KY D + ++AG AS I +G K P +++ + + G+PN P Sbjct: 57 QGRKYFD-SGDYALNKAGKASDSGITCIG--------KEIPSPDTIPHRVVSAGSPNKEP 107 Query: 387 SLHSRSTSRDLP 422 SLH++ S P Sbjct: 108 SLHTKRPSESSP 119 >SPAC13G7.10 |mug152||transcription factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 27.9 bits (59), Expect = 1.8 Identities = 14/34 (41%), Positives = 20/34 (58%) Frame = +2 Query: 371 TQRAAVAPFAFHIQGLTNGLEPPLTAAVLSACAL 472 T + +V PF F IQ LEPPL++ L++ L Sbjct: 326 TSQNSVQPFPFSIQQPPLHLEPPLSSNTLNSSTL 359 >SPAC4A8.11c |fas2|lsd1|fatty acid synthase alpha subunit Lsd1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1842 Score = 26.2 bits (55), Expect = 5.6 Identities = 20/66 (30%), Positives = 30/66 (45%), Gaps = 2/66 (3%) Frame = +1 Query: 58 TTSEKKFWTAEEKRAKEAYSEDSLRRLGYLDALAGLPLGKMNDESPEL--CARARSTGSG 231 T + K TA +A Y+ D+L + A P K +++ L ARA + +G Sbjct: 1628 TYNAYKAKTAARYKASYRYTHDALVYNNLVRAKDSPPYTKEQEKAVYLNPLARASKSKAG 1687 Query: 232 EWTAPA 249 WT PA Sbjct: 1688 TWTFPA 1693 >SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 583 Score = 25.4 bits (53), Expect = 9.8 Identities = 16/44 (36%), Positives = 21/44 (47%), Gaps = 1/44 (2%) Frame = +1 Query: 412 GTYQWTGASIDSRCTECLCPPLLTTE-RSKKKC*NEHVHYNCLD 540 G + ++G D RC CL L E R K+C N H C+D Sbjct: 514 GVFPFSGT--DERCLVCLSNFELNDECRRLKQC-NHFFHRECID 554 >SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1323 Score = 25.4 bits (53), Expect = 9.8 Identities = 10/25 (40%), Positives = 16/25 (64%) Frame = -2 Query: 426 PLVSPWMWNANGATAARWVRRGPHV 352 P+ S + W+ NGAT +W + PH+ Sbjct: 358 PVNSFYDWH-NGATQVKWNYKNPHI 381 >SPAC13G7.04c |mac1||membrane anchored protein Mac1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 756 Score = 25.4 bits (53), Expect = 9.8 Identities = 23/71 (32%), Positives = 31/71 (43%) Frame = -3 Query: 272 NTGQSCPRAGAVHSPLPVLLALAHNSGDSSFIFPKGSPARASR*PNLLKLSSEYASLARF 93 NT QS AGA+ S L +LAL S S I K AR + S Y + ++ Sbjct: 145 NTWQSYVTAGAIGSDLIAILALCLRSVSISRIGQKS--ARLEHVDTMNSSYSSYKTDVKY 202 Query: 92 SSAVQNFFSDV 60 A+ + S V Sbjct: 203 PLALDDKLSSV 213 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,318,282 Number of Sequences: 5004 Number of extensions: 69456 Number of successful extensions: 201 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 195 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 201 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 400438000 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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