BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1453
(820 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC10F6.16 |mug134||endosulphine family protein|Schizosaccharom... 31 0.20
SPAC13G7.10 |mug152||transcription factor |Schizosaccharomyces p... 28 1.8
SPAC4A8.11c |fas2|lsd1|fatty acid synthase alpha subunit Lsd1 |S... 26 5.6
SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 9.8
SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces p... 25 9.8
SPAC13G7.04c |mac1||membrane anchored protein Mac1 |Schizosaccha... 25 9.8
>SPAC10F6.16 |mug134||endosulphine family
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 139
Score = 31.1 bits (67), Expect = 0.20
Identities = 22/72 (30%), Positives = 34/72 (47%)
Frame = +3
Query: 207 QGEKYRKWRVDCSCSRAGLASVLAICALGATWRLCCRKRSPDEESLDY*HAARGAPNAPP 386
QG KY D + ++AG AS I +G K P +++ + + G+PN P
Sbjct: 57 QGRKYFD-SGDYALNKAGKASDSGITCIG--------KEIPSPDTIPHRVVSAGSPNKEP 107
Query: 387 SLHSRSTSRDLP 422
SLH++ S P
Sbjct: 108 SLHTKRPSESSP 119
>SPAC13G7.10 |mug152||transcription factor |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 390
Score = 27.9 bits (59), Expect = 1.8
Identities = 14/34 (41%), Positives = 20/34 (58%)
Frame = +2
Query: 371 TQRAAVAPFAFHIQGLTNGLEPPLTAAVLSACAL 472
T + +V PF F IQ LEPPL++ L++ L
Sbjct: 326 TSQNSVQPFPFSIQQPPLHLEPPLSSNTLNSSTL 359
>SPAC4A8.11c |fas2|lsd1|fatty acid synthase alpha subunit Lsd1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1842
Score = 26.2 bits (55), Expect = 5.6
Identities = 20/66 (30%), Positives = 30/66 (45%), Gaps = 2/66 (3%)
Frame = +1
Query: 58 TTSEKKFWTAEEKRAKEAYSEDSLRRLGYLDALAGLPLGKMNDESPEL--CARARSTGSG 231
T + K TA +A Y+ D+L + A P K +++ L ARA + +G
Sbjct: 1628 TYNAYKAKTAARYKASYRYTHDALVYNNLVRAKDSPPYTKEQEKAVYLNPLARASKSKAG 1687
Query: 232 EWTAPA 249
WT PA
Sbjct: 1688 TWTFPA 1693
>SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 583
Score = 25.4 bits (53), Expect = 9.8
Identities = 16/44 (36%), Positives = 21/44 (47%), Gaps = 1/44 (2%)
Frame = +1
Query: 412 GTYQWTGASIDSRCTECLCPPLLTTE-RSKKKC*NEHVHYNCLD 540
G + ++G D RC CL L E R K+C N H C+D
Sbjct: 514 GVFPFSGT--DERCLVCLSNFELNDECRRLKQC-NHFFHRECID 554
>SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1323
Score = 25.4 bits (53), Expect = 9.8
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = -2
Query: 426 PLVSPWMWNANGATAARWVRRGPHV 352
P+ S + W+ NGAT +W + PH+
Sbjct: 358 PVNSFYDWH-NGATQVKWNYKNPHI 381
>SPAC13G7.04c |mac1||membrane anchored protein Mac1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 756
Score = 25.4 bits (53), Expect = 9.8
Identities = 23/71 (32%), Positives = 31/71 (43%)
Frame = -3
Query: 272 NTGQSCPRAGAVHSPLPVLLALAHNSGDSSFIFPKGSPARASR*PNLLKLSSEYASLARF 93
NT QS AGA+ S L +LAL S S I K AR + S Y + ++
Sbjct: 145 NTWQSYVTAGAIGSDLIAILALCLRSVSISRIGQKS--ARLEHVDTMNSSYSSYKTDVKY 202
Query: 92 SSAVQNFFSDV 60
A+ + S V
Sbjct: 203 PLALDDKLSSV 213
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,318,282
Number of Sequences: 5004
Number of extensions: 69456
Number of successful extensions: 201
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 195
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 201
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 400438000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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