BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--1443 (846 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1215.01 |shy1||SURF-family protein Shy1|Schizosaccharomyces ... 66 6e-12 SPAC1834.05 |alg9||mannosyltransferase complex subunit Alg9 |Sch... 30 0.36 SPAC23H3.11c |||glucosidase |Schizosaccharomyces pombe|chr 1|||M... 27 2.5 >SPBC1215.01 |shy1||SURF-family protein Shy1|Schizosaccharomyces pombe|chr 2|||Manual Length = 290 Score = 66.1 bits (154), Expect = 6e-12 Identities = 28/75 (37%), Positives = 50/75 (66%), Gaps = 2/75 (2%) Frame = +1 Query: 28 ILLMIPVTSFTLGSWQVYRWQWKLGLIDMMQAKSNAVPIDMPKDFSE--LEKMEYLPVKV 201 +L +P+ +F LG+WQV R +WK+G+I+ + + I +PK +E +K+E+ V + Sbjct: 42 LLSAVPIVTFALGTWQVKRREWKMGIINTLTERLQQPAILLPKTVTEQDTKKLEWTRVLL 101 Query: 202 KGEFLHEKEILIGPR 246 +G F H++E+L+GPR Sbjct: 102 RGVFCHDQEMLVGPR 116 Score = 61.3 bits (142), Expect = 2e-10 Identities = 32/67 (47%), Positives = 43/67 (64%), Gaps = 1/67 (1%) Frame = +3 Query: 315 GWLVITPFKLADTGEVILINRGWIHQNLRPKEKREP-SLIKGPVELTGVVRLTEKRAPFM 491 G+ V+TPF L D G IL+NRGWI ++ + R+P SL KGPV + G++R + FM Sbjct: 123 GYHVVTPFIL-DDGRRILVNRGWIARSFAEQSSRDPSSLPKGPVVIEGLLRQHTDKPRFM 181 Query: 492 PKNNPEK 512 KN PEK Sbjct: 182 MKNEPEK 188 Score = 39.1 bits (87), Expect = 8e-04 Identities = 16/40 (40%), Positives = 25/40 (62%) Frame = +2 Query: 608 GWPIPNQTRVTLRNEHFSYIVTWYSLFAFTSIMWHRFFIR 727 G P+ + +V + N H YI+TWYSL ++IM + +F R Sbjct: 229 GLPLGHPLKVEIFNSHTEYIITWYSLSVVSAIMLYVYFKR 268 >SPAC1834.05 |alg9||mannosyltransferase complex subunit Alg9 |Schizosaccharomyces pombe|chr 1|||Manual Length = 577 Score = 30.3 bits (65), Expect = 0.36 Identities = 15/55 (27%), Positives = 28/55 (50%), Gaps = 2/55 (3%) Frame = +3 Query: 291 VSDP--KKNQGWLVITPFKLADTGEVILINRGWIHQNLRPKEKREPSLIKGPVEL 449 V++P K+ GW+ + + DT + + R + + PK R L+K PV++ Sbjct: 510 VNEPIYSKSDGWIPVMVYPFIDTKQTPFMGRAFAVPFIEPKWGRYEILVKKPVKI 564 >SPAC23H3.11c |||glucosidase |Schizosaccharomyces pombe|chr 1|||Manual Length = 629 Score = 27.5 bits (58), Expect = 2.5 Identities = 14/37 (37%), Positives = 20/37 (54%), Gaps = 1/37 (2%) Frame = +2 Query: 524 YRDLDQMSAHIGC-LPIWLDAKGIPDPPTGWPIPNQT 631 Y+D D ++HIGC P + K I + P + PN T Sbjct: 573 YQDPDDTNSHIGCDPPGYPTTKYIEEHPLAYKNPNAT 609 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,621,433 Number of Sequences: 5004 Number of extensions: 78705 Number of successful extensions: 190 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 179 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 188 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 418457710 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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