BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1443
(846 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1215.01 |shy1||SURF-family protein Shy1|Schizosaccharomyces ... 66 6e-12
SPAC1834.05 |alg9||mannosyltransferase complex subunit Alg9 |Sch... 30 0.36
SPAC23H3.11c |||glucosidase |Schizosaccharomyces pombe|chr 1|||M... 27 2.5
>SPBC1215.01 |shy1||SURF-family protein Shy1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 290
Score = 66.1 bits (154), Expect = 6e-12
Identities = 28/75 (37%), Positives = 50/75 (66%), Gaps = 2/75 (2%)
Frame = +1
Query: 28 ILLMIPVTSFTLGSWQVYRWQWKLGLIDMMQAKSNAVPIDMPKDFSE--LEKMEYLPVKV 201
+L +P+ +F LG+WQV R +WK+G+I+ + + I +PK +E +K+E+ V +
Sbjct: 42 LLSAVPIVTFALGTWQVKRREWKMGIINTLTERLQQPAILLPKTVTEQDTKKLEWTRVLL 101
Query: 202 KGEFLHEKEILIGPR 246
+G F H++E+L+GPR
Sbjct: 102 RGVFCHDQEMLVGPR 116
Score = 61.3 bits (142), Expect = 2e-10
Identities = 32/67 (47%), Positives = 43/67 (64%), Gaps = 1/67 (1%)
Frame = +3
Query: 315 GWLVITPFKLADTGEVILINRGWIHQNLRPKEKREP-SLIKGPVELTGVVRLTEKRAPFM 491
G+ V+TPF L D G IL+NRGWI ++ + R+P SL KGPV + G++R + FM
Sbjct: 123 GYHVVTPFIL-DDGRRILVNRGWIARSFAEQSSRDPSSLPKGPVVIEGLLRQHTDKPRFM 181
Query: 492 PKNNPEK 512
KN PEK
Sbjct: 182 MKNEPEK 188
Score = 39.1 bits (87), Expect = 8e-04
Identities = 16/40 (40%), Positives = 25/40 (62%)
Frame = +2
Query: 608 GWPIPNQTRVTLRNEHFSYIVTWYSLFAFTSIMWHRFFIR 727
G P+ + +V + N H YI+TWYSL ++IM + +F R
Sbjct: 229 GLPLGHPLKVEIFNSHTEYIITWYSLSVVSAIMLYVYFKR 268
>SPAC1834.05 |alg9||mannosyltransferase complex subunit Alg9
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 577
Score = 30.3 bits (65), Expect = 0.36
Identities = 15/55 (27%), Positives = 28/55 (50%), Gaps = 2/55 (3%)
Frame = +3
Query: 291 VSDP--KKNQGWLVITPFKLADTGEVILINRGWIHQNLRPKEKREPSLIKGPVEL 449
V++P K+ GW+ + + DT + + R + + PK R L+K PV++
Sbjct: 510 VNEPIYSKSDGWIPVMVYPFIDTKQTPFMGRAFAVPFIEPKWGRYEILVKKPVKI 564
>SPAC23H3.11c |||glucosidase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 629
Score = 27.5 bits (58), Expect = 2.5
Identities = 14/37 (37%), Positives = 20/37 (54%), Gaps = 1/37 (2%)
Frame = +2
Query: 524 YRDLDQMSAHIGC-LPIWLDAKGIPDPPTGWPIPNQT 631
Y+D D ++HIGC P + K I + P + PN T
Sbjct: 573 YQDPDDTNSHIGCDPPGYPTTKYIEEHPLAYKNPNAT 609
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,621,433
Number of Sequences: 5004
Number of extensions: 78705
Number of successful extensions: 190
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 188
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 418457710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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