Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1394
(604 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 124 8e-30
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 82 5e-17
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 81 1e-16
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 75 6e-15
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 2.1
SPBC21H7.02 |taf10||transcription factor TFIID complex subunit T... 27 2.8
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 2.8
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 26 3.7
SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 26 3.7
SPCC1223.03c |gut2||glycerol-3-phosphate dehydrogenase Gut2|Schi... 26 4.9
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 4.9
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 124 bits (300), Expect = 8e-30
Identities = 53/67 (79%), Positives = 61/67 (91%)
Frame = +1
Query: 253 LEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESC 432
LEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVVR+EAE+C
Sbjct: 68 LEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEAC 127
Query: 433 DCLQGFQ 453
D LQGFQ
Sbjct: 128 DALQGFQ 134
Score = 121 bits (292), Expect = 7e-29
Identities = 51/70 (72%), Positives = 59/70 (84%)
Frame = +2
Query: 53 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 232
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 233 PRAILVDWSP 262
PRA+LVD P
Sbjct: 61 PRAVLVDLEP 70
Score = 58.4 bits (135), Expect = 7e-10
Identities = 25/31 (80%), Positives = 28/31 (90%)
Frame = +3
Query: 510 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYN 602
KIREEYPDR+M T+SV P+PK SDTVVEPYN
Sbjct: 154 KIREEYPDRMMATFSVAPAPKSSDTVVEPYN 184
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 82.2 bits (194), Expect = 5e-17
Identities = 33/66 (50%), Positives = 48/66 (72%)
Frame = +1
Query: 253 LEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESC 432
LEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++DSVL+ +R+ A++C
Sbjct: 74 LEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNC 133
Query: 433 DCLQGF 450
LQGF
Sbjct: 134 SGLQGF 139
Score = 56.8 bits (131), Expect = 2e-09
Identities = 28/76 (36%), Positives = 40/76 (52%), Gaps = 6/76 (7%)
Frame = +2
Query: 53 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 214
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 215 SGGKYVPRAILVDWSP 262
GK+VPR+I VD P
Sbjct: 61 GQGKFVPRSIYVDLEP 76
Score = 33.1 bits (72), Expect = 0.032
Identities = 14/31 (45%), Positives = 21/31 (67%)
Frame = +3
Query: 510 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYN 602
++ EY + +SV P+P+VS +VVEPYN
Sbjct: 160 RLNMEYGKKSNLQFSVYPAPQVSTSVVEPYN 190
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 81.0 bits (191), Expect = 1e-16
Identities = 33/66 (50%), Positives = 46/66 (69%)
Frame = +1
Query: 253 LEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESC 432
LEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D +R+ A++C
Sbjct: 70 LEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNC 129
Query: 433 DCLQGF 450
LQGF
Sbjct: 130 SGLQGF 135
Score = 62.9 bits (146), Expect = 3e-11
Identities = 31/72 (43%), Positives = 41/72 (56%), Gaps = 2/72 (2%)
Frame = +2
Query: 53 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 226
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 227 YVPRAILVDWSP 262
YVPR+I VD P
Sbjct: 61 YVPRSIYVDLEP 72
Score = 33.5 bits (73), Expect = 0.024
Identities = 14/31 (45%), Positives = 21/31 (67%)
Frame = +3
Query: 510 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYN 602
++ EY + +SV P+P+VS +VVEPYN
Sbjct: 156 RLAMEYTKKSKLQFSVYPAPQVSTSVVEPYN 186
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 75.4 bits (177), Expect = 6e-15
Identities = 31/69 (44%), Positives = 48/69 (69%)
Frame = +2
Query: 56 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 235
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 236 RAILVDWSP 262
RAIL+D P
Sbjct: 63 RAILIDLEP 71
Score = 57.6 bits (133), Expect = 1e-09
Identities = 23/68 (33%), Positives = 44/68 (64%), Gaps = 2/68 (2%)
Frame = +1
Query: 253 LEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDVVRKEAE 426
LEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D++ +EA+
Sbjct: 69 LEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREAD 127
Query: 427 SCDCLQGF 450
D L+GF
Sbjct: 128 GSDSLEGF 135
Score = 39.5 bits (88), Expect = 4e-04
Identities = 17/32 (53%), Positives = 23/32 (71%), Gaps = 1/32 (3%)
Frame = +3
Query: 510 KIREEYPDRIMNTYSVVP-SPKVSDTVVEPYN 602
++ + YP +I+ TYSV P S VSD VV+PYN
Sbjct: 156 RLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYN 187
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 2.1
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +2
Query: 92 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 193
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPBC21H7.02 |taf10||transcription factor TFIID complex subunit
Taf10 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 215
Score = 26.6 bits (56), Expect = 2.8
Identities = 14/53 (26%), Positives = 25/53 (47%), Gaps = 3/53 (5%)
Frame = +3
Query: 450 PTDTLARRRHRFRYGHPPHLKIREEYPDRIMNTYSVVP---SPKVSDTVVEPY 599
P +R RH Y PHL++R+ D+ + + SP + D +++ Y
Sbjct: 63 PKSDDSRERHGSNYVIEPHLELRDMAKDKTLENFLAQMDDYSPLIPDVLLDYY 115
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 2.8
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +1
Query: 286 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 387
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 26.2 bits (55), Expect = 3.7
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +1
Query: 355 AKGHYTEGAELVDSVLDVVRKEAESCDCLQ 444
A+GH G ELV + D +RK++E+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr
2|||Manual
Length = 807
Score = 26.2 bits (55), Expect = 3.7
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = -3
Query: 428 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPK 291
++A+ RTTS T+ +PS L P P + S R+ CPK
Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432
>SPCC1223.03c |gut2||glycerol-3-phosphate dehydrogenase
Gut2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 649
Score = 25.8 bits (54), Expect = 4.9
Identities = 13/54 (24%), Positives = 26/54 (48%)
Frame = -2
Query: 393 IDELSTLRVVSLGPVVAGAGLSEDEVVRTEDLSERSRADRVHGAGLQSTRMARG 232
++++S V+ +G G G++ D R ++ + D +ST+MA G
Sbjct: 62 VEDISKFDVLIIGGGATGTGVAVDASTRGLNVCLLEKTDFASETSSKSTKMAHG 115
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.8 bits (54), Expect = 4.9
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +1
Query: 307 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 438
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,263,275
Number of Sequences: 5004
Number of extensions: 42474
Number of successful extensions: 142
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 125
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 138
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 264253462
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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