BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--1376 (830 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC8C9.09c |mug129||sequence orphan|Schizosaccharomyces pombe|c... 29 0.81 SPAC3A12.03c |mug145||ubiquitin-protein ligase E3 |Schizosacchar... 27 4.3 SPAC12B10.08c |||mitochondrial tRNA|Schizosaccharomyces pombe|ch... 26 5.7 SPAC56F8.06c |alg10||dolichyl-phosphate-glucose-glycolipid alpha... 26 5.7 SPBC20F10.03 |||conserved eukaryotic protein|Schizosaccharomyces... 26 5.7 SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 26 7.5 SPAC56F8.10 |met9|met5|methylenetetrahydrofolate reductase Met9|... 25 10.0 SPAC17A5.01 |pex6||peroxin-6 |Schizosaccharomyces pombe|chr 1|||... 25 10.0 SPBC15D4.07c |atg9|apg9|autophagy associated protein Atg9 |Schiz... 25 10.0 SPCC736.15 |||protein kinase inhibitor |Schizosaccharomyces pomb... 25 10.0 >SPAC8C9.09c |mug129||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 302 Score = 29.1 bits (62), Expect = 0.81 Identities = 10/37 (27%), Positives = 22/37 (59%) Frame = -1 Query: 170 PKKHCLTRVELLYFSCLKPFGNCQHTKKSRVEFATSL 60 P +HC + ++ +Y +C+ P + + +V+F TS+ Sbjct: 243 PDEHCNSWIQRIYITCVLPRNHYIYLLNEQVDFLTSI 279 >SPAC3A12.03c |mug145||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 309 Score = 26.6 bits (56), Expect = 4.3 Identities = 18/62 (29%), Positives = 29/62 (46%), Gaps = 3/62 (4%) Frame = -1 Query: 323 DNSFLLFAINIANFIWFVNVVFHFFC*YTERKRRLFTYFDYKVKT---DDEIPIPKKHCL 153 + LLFA+ I + F+N F + C R YF + ++ DDE P+ + H + Sbjct: 20 NTEILLFALVIILSVIFINFFFFYLC-------RCCVYFYHTLENQEGDDERPLIQHHMV 72 Query: 152 TR 147 R Sbjct: 73 NR 74 >SPAC12B10.08c |||mitochondrial tRNA|Schizosaccharomyces pombe|chr 1|||Manual Length = 456 Score = 26.2 bits (55), Expect = 5.7 Identities = 18/48 (37%), Positives = 25/48 (52%), Gaps = 1/48 (2%) Frame = -1 Query: 167 KKHC-LTRVELLYFSCLKPFGNCQHTKKSRVEFATSLITDEKRLGTRK 27 KKH LT+ ELL CL +C+ K+S V + + D K+L K Sbjct: 279 KKHSNLTKEELL-LRCLTLTTSCRQIKRSSVVSLCNSVFDGKKLTIAK 325 >SPAC56F8.06c |alg10||dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase Alg10|Schizosaccharomyces pombe|chr 1|||Manual Length = 445 Score = 26.2 bits (55), Expect = 5.7 Identities = 12/30 (40%), Positives = 20/30 (66%), Gaps = 2/30 (6%) Frame = +3 Query: 162 FLGNRDFIICFYFVIEISK--QSSFTFSIL 245 +LG ++I +YF ++ISK +S TF +L Sbjct: 341 YLGPFSYLILYYFFLDISKLQMTSLTFFLL 370 >SPBC20F10.03 |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 26.2 bits (55), Expect = 5.7 Identities = 13/36 (36%), Positives = 20/36 (55%) Frame = +3 Query: 249 KKMENDINKPYKICDVNREKKKGIVAMSLEDLLIKV 356 K+ E + K Y IC + K+ SLE+LL+K+ Sbjct: 67 KEREEHLEKIYVICTRHYAKRISENVASLEELLLKI 102 >SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|Schizosaccharomyces pombe|chr 1|||Manual Length = 2397 Score = 25.8 bits (54), Expect = 7.5 Identities = 11/25 (44%), Positives = 17/25 (68%), Gaps = 2/25 (8%) Frame = -1 Query: 581 QQDSPLV--LYHPKLYHRSDLTIPD 513 + D+PL LYHP ++ SD+ IP+ Sbjct: 1795 ESDTPLQNKLYHPGMWSSSDIRIPN 1819 >SPAC56F8.10 |met9|met5|methylenetetrahydrofolate reductase Met9|Schizosaccharomyces pombe|chr 1|||Manual Length = 603 Score = 25.4 bits (53), Expect = 10.0 Identities = 10/32 (31%), Positives = 21/32 (65%) Frame = +3 Query: 327 MSLEDLLIKVPEKLGMPSENLTVVLESDGTEV 422 M+LE + + E+LG+ ENL +++++ E+ Sbjct: 278 MNLEKAVKMIIERLGLLDENLAPIVDTNNVEL 309 >SPAC17A5.01 |pex6||peroxin-6 |Schizosaccharomyces pombe|chr 1|||Manual Length = 948 Score = 25.4 bits (53), Expect = 10.0 Identities = 23/106 (21%), Positives = 49/106 (46%), Gaps = 2/106 (1%) Frame = +3 Query: 207 EISKQSSFTFSILTKKMEN--DINKPYKICDVNREKKKGIVAMSLEDLLIKVPEKLGMPS 380 E SK S T K++ D+N+ K C N + A+ + +L + K + Sbjct: 829 EESKASMLRALTKTFKLDETIDLNEIAKNCHPNFTGAD-MYALCSDAVLSAIKRK----T 883 Query: 381 ENLTVVLESDGTEVDDEEYFSTLDPDTSLMILHGNEKWVPNMPSVR 518 + +++++ GT++ EE+F + SL + E ++ ++ +R Sbjct: 884 NEIDLLIQASGTDLSTEEFFKRNENQDSLELRITKEDFLTSLKKLR 929 >SPBC15D4.07c |atg9|apg9|autophagy associated protein Atg9 |Schizosaccharomyces pombe|chr 2|||Manual Length = 702 Score = 25.4 bits (53), Expect = 10.0 Identities = 13/35 (37%), Positives = 20/35 (57%) Frame = -3 Query: 744 SRRILPDASLICSKIILLSRSVTMSAKLSGSISDN 640 SR I+PD +L+ + L R +T + + G SDN Sbjct: 552 SRSIIPDETLVFAPEKALRRVITFTHYMPGWWSDN 586 >SPCC736.15 |||protein kinase inhibitor |Schizosaccharomyces pombe|chr 3|||Manual Length = 351 Score = 25.4 bits (53), Expect = 10.0 Identities = 27/93 (29%), Positives = 43/93 (46%), Gaps = 6/93 (6%) Frame = +3 Query: 282 KICDVNREKKKGIVAMSLEDLLIKVPEKLGMPSENLTVVLES-DGTEVDDEEYFSTLDP- 455 ++ + REK K + +L D L + EKL + + +L D T V E D Sbjct: 186 QLSNTTREKFKQAMTFNL-DALHEHAEKLNLIATYGRHLLNLIDDTPVTPGEARPAYDGY 244 Query: 456 DTSLMIL----HGNEKWVPNMPSVRYR*IRPMI 542 +TS I+ H WVP+ P+V + ++P I Sbjct: 245 ETSRQIVMDAEHALSSWVPSQPAVNF--VKPQI 275 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,560,584 Number of Sequences: 5004 Number of extensions: 77164 Number of successful extensions: 216 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 205 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 216 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 408446760 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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