BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1376
(830 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC8C9.09c |mug129||sequence orphan|Schizosaccharomyces pombe|c... 29 0.81
SPAC3A12.03c |mug145||ubiquitin-protein ligase E3 |Schizosacchar... 27 4.3
SPAC12B10.08c |||mitochondrial tRNA|Schizosaccharomyces pombe|ch... 26 5.7
SPAC56F8.06c |alg10||dolichyl-phosphate-glucose-glycolipid alpha... 26 5.7
SPBC20F10.03 |||conserved eukaryotic protein|Schizosaccharomyces... 26 5.7
SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 26 7.5
SPAC56F8.10 |met9|met5|methylenetetrahydrofolate reductase Met9|... 25 10.0
SPAC17A5.01 |pex6||peroxin-6 |Schizosaccharomyces pombe|chr 1|||... 25 10.0
SPBC15D4.07c |atg9|apg9|autophagy associated protein Atg9 |Schiz... 25 10.0
SPCC736.15 |||protein kinase inhibitor |Schizosaccharomyces pomb... 25 10.0
>SPAC8C9.09c |mug129||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 302
Score = 29.1 bits (62), Expect = 0.81
Identities = 10/37 (27%), Positives = 22/37 (59%)
Frame = -1
Query: 170 PKKHCLTRVELLYFSCLKPFGNCQHTKKSRVEFATSL 60
P +HC + ++ +Y +C+ P + + +V+F TS+
Sbjct: 243 PDEHCNSWIQRIYITCVLPRNHYIYLLNEQVDFLTSI 279
>SPAC3A12.03c |mug145||ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 309
Score = 26.6 bits (56), Expect = 4.3
Identities = 18/62 (29%), Positives = 29/62 (46%), Gaps = 3/62 (4%)
Frame = -1
Query: 323 DNSFLLFAINIANFIWFVNVVFHFFC*YTERKRRLFTYFDYKVKT---DDEIPIPKKHCL 153
+ LLFA+ I + F+N F + C R YF + ++ DDE P+ + H +
Sbjct: 20 NTEILLFALVIILSVIFINFFFFYLC-------RCCVYFYHTLENQEGDDERPLIQHHMV 72
Query: 152 TR 147
R
Sbjct: 73 NR 74
>SPAC12B10.08c |||mitochondrial tRNA|Schizosaccharomyces pombe|chr
1|||Manual
Length = 456
Score = 26.2 bits (55), Expect = 5.7
Identities = 18/48 (37%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
Frame = -1
Query: 167 KKHC-LTRVELLYFSCLKPFGNCQHTKKSRVEFATSLITDEKRLGTRK 27
KKH LT+ ELL CL +C+ K+S V + + D K+L K
Sbjct: 279 KKHSNLTKEELL-LRCLTLTTSCRQIKRSSVVSLCNSVFDGKKLTIAK 325
>SPAC56F8.06c |alg10||dolichyl-phosphate-glucose-glycolipid
alpha-glucosyltransferase Alg10|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 445
Score = 26.2 bits (55), Expect = 5.7
Identities = 12/30 (40%), Positives = 20/30 (66%), Gaps = 2/30 (6%)
Frame = +3
Query: 162 FLGNRDFIICFYFVIEISK--QSSFTFSIL 245
+LG ++I +YF ++ISK +S TF +L
Sbjct: 341 YLGPFSYLILYYFFLDISKLQMTSLTFFLL 370
>SPBC20F10.03 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 446
Score = 26.2 bits (55), Expect = 5.7
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +3
Query: 249 KKMENDINKPYKICDVNREKKKGIVAMSLEDLLIKV 356
K+ E + K Y IC + K+ SLE+LL+K+
Sbjct: 67 KEREEHLEKIYVICTRHYAKRISENVASLEELLLKI 102
>SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase
Mok11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2397
Score = 25.8 bits (54), Expect = 7.5
Identities = 11/25 (44%), Positives = 17/25 (68%), Gaps = 2/25 (8%)
Frame = -1
Query: 581 QQDSPLV--LYHPKLYHRSDLTIPD 513
+ D+PL LYHP ++ SD+ IP+
Sbjct: 1795 ESDTPLQNKLYHPGMWSSSDIRIPN 1819
>SPAC56F8.10 |met9|met5|methylenetetrahydrofolate reductase
Met9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 603
Score = 25.4 bits (53), Expect = 10.0
Identities = 10/32 (31%), Positives = 21/32 (65%)
Frame = +3
Query: 327 MSLEDLLIKVPEKLGMPSENLTVVLESDGTEV 422
M+LE + + E+LG+ ENL +++++ E+
Sbjct: 278 MNLEKAVKMIIERLGLLDENLAPIVDTNNVEL 309
>SPAC17A5.01 |pex6||peroxin-6 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 948
Score = 25.4 bits (53), Expect = 10.0
Identities = 23/106 (21%), Positives = 49/106 (46%), Gaps = 2/106 (1%)
Frame = +3
Query: 207 EISKQSSFTFSILTKKMEN--DINKPYKICDVNREKKKGIVAMSLEDLLIKVPEKLGMPS 380
E SK S T K++ D+N+ K C N + A+ + +L + K +
Sbjct: 829 EESKASMLRALTKTFKLDETIDLNEIAKNCHPNFTGAD-MYALCSDAVLSAIKRK----T 883
Query: 381 ENLTVVLESDGTEVDDEEYFSTLDPDTSLMILHGNEKWVPNMPSVR 518
+ +++++ GT++ EE+F + SL + E ++ ++ +R
Sbjct: 884 NEIDLLIQASGTDLSTEEFFKRNENQDSLELRITKEDFLTSLKKLR 929
>SPBC15D4.07c |atg9|apg9|autophagy associated protein Atg9
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 702
Score = 25.4 bits (53), Expect = 10.0
Identities = 13/35 (37%), Positives = 20/35 (57%)
Frame = -3
Query: 744 SRRILPDASLICSKIILLSRSVTMSAKLSGSISDN 640
SR I+PD +L+ + L R +T + + G SDN
Sbjct: 552 SRSIIPDETLVFAPEKALRRVITFTHYMPGWWSDN 586
>SPCC736.15 |||protein kinase inhibitor |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 351
Score = 25.4 bits (53), Expect = 10.0
Identities = 27/93 (29%), Positives = 43/93 (46%), Gaps = 6/93 (6%)
Frame = +3
Query: 282 KICDVNREKKKGIVAMSLEDLLIKVPEKLGMPSENLTVVLES-DGTEVDDEEYFSTLDP- 455
++ + REK K + +L D L + EKL + + +L D T V E D
Sbjct: 186 QLSNTTREKFKQAMTFNL-DALHEHAEKLNLIATYGRHLLNLIDDTPVTPGEARPAYDGY 244
Query: 456 DTSLMIL----HGNEKWVPNMPSVRYR*IRPMI 542
+TS I+ H WVP+ P+V + ++P I
Sbjct: 245 ETSRQIVMDAEHALSSWVPSQPAVNF--VKPQI 275
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,560,584
Number of Sequences: 5004
Number of extensions: 77164
Number of successful extensions: 216
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 205
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 216
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 408446760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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