Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--1362
         (846 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g11820.2 68417.m01882 hydroxymethylglutaryl-CoA synthase / HM...   101   5e-22
At4g11820.1 68417.m01881 hydroxymethylglutaryl-CoA synthase / HM...   101   5e-22
At4g38110.1 68417.m05382 expressed protein                             30   1.7  
At1g08280.1 68414.m00914 glycosyl transferase family 29 protein ...    30   2.2  
At4g09900.1 68417.m01622 hydrolase, alpha/beta fold family prote...    29   5.1  
At4g39290.1 68417.m05564 kelch repeat-containing F-box family pr...    28   6.8  
At1g17230.1 68414.m02099 leucine-rich repeat family protein / pr...    28   6.8  
At2g04620.1 68415.m00470 cation efflux family protein potential ...    28   9.0  
At1g33990.1 68414.m04214 hydrolase, alpha/beta fold family prote...    28   9.0  

>At4g11820.2 68417.m01882 hydroxymethylglutaryl-CoA synthase /
           HMG-CoA synthase / 3-hydroxy-3-methylglutaryl coenzyme A
           synthase identical to Hydroxymethylglutaryl-CoA synthase
           (EC 4.1.3.5) (HMG-CoA synthase)
           (3-hydroxy-3-methylglutaryl coenzyme A synthase)
           (Swiss-Prot:P54873) [Arabidopsis thaliana]
          Length = 461

 Score =  101 bits (243), Expect = 5e-22
 Identities = 46/83 (55%), Positives = 57/83 (68%)
 Frame = +3

Query: 3   YMTHAYDFYKPDLSSEFPYVDGKLSIQCYLNALDNCYNLFSKKMRKTDPNFKGLLSLDGM 182
           +M H YDFYKP+L+SE+P VDGKLS  CYL ALD+CY     K  K +     +   D +
Sbjct: 185 HMAHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKHLCNKFEKIEGKEFSINDADYI 244

Query: 183 LFHSPYCKLVQKSLARVCFNDFL 251
           +FHSPY KLVQKS AR+ +NDFL
Sbjct: 245 VFHSPYNKLVQKSFARLLYNDFL 267



 Score = 58.8 bits (136), Expect = 4e-09
 Identities = 35/104 (33%), Positives = 55/104 (52%), Gaps = 2/104 (1%)
 Frame = +1

Query: 499 EEVSLFSYGSGLASTMYSINVCHDMSTGSKLEKLISSLHETVALLDKRQSVEPSKFSDIM 678
           + V +FSYGSG  +TM+S+ + +D      +   I+S+ +    L  R    P KF + M
Sbjct: 350 KRVVMFSYGSGSTATMFSLRL-NDNKPPFSISN-IASVMDVGGKLKARHEYAPEKFVETM 407

Query: 679 DVRTKNYHSAPYEPT--GSLDVLFPGTYYL*KIDDQRRRTYDRK 804
            +    Y +  +  T  G +D+L PGTYYL ++D   RR Y +K
Sbjct: 408 KLMEHRYGAKDFVTTKEGIIDLLAPGTYYLKEVDSLYRRFYGKK 451



 Score = 53.2 bits (122), Expect = 2e-07
 Identities = 31/102 (30%), Positives = 54/102 (52%)
 Frame = +2

Query: 212 SKIISKSVLQ*FSELPAEEREKQFPGLSEFSNHKLEDTYFDREVEKAFMTYSLSLFEEKT 391
           ++++    L+  S +    +EK  P    +S+  L+++Y  R++EK     +   ++ K 
Sbjct: 259 ARLLYNDFLRNASSIDEAAKEKFTP----YSSLTLDESYQSRDLEKVSQQIAKPFYDAKV 314

Query: 392 KPSLHIARNVGNMYTPSLYGGLVSYLISKSPDQLIGKKFLCF 517
           +P+  I + VGNMYT SLY    S LI K  + L GK+ + F
Sbjct: 315 QPTTLIPKEVGNMYTASLYAAFAS-LIHKKHNDLAGKRVVMF 355


>At4g11820.1 68417.m01881 hydroxymethylglutaryl-CoA synthase /
           HMG-CoA synthase / 3-hydroxy-3-methylglutaryl coenzyme A
           synthase identical to Hydroxymethylglutaryl-CoA synthase
           (EC 4.1.3.5) (HMG-CoA synthase)
           (3-hydroxy-3-methylglutaryl coenzyme A synthase)
           (Swiss-Prot:P54873) [Arabidopsis thaliana]
          Length = 406

 Score =  101 bits (243), Expect = 5e-22
 Identities = 46/83 (55%), Positives = 57/83 (68%)
 Frame = +3

Query: 3   YMTHAYDFYKPDLSSEFPYVDGKLSIQCYLNALDNCYNLFSKKMRKTDPNFKGLLSLDGM 182
           +M H YDFYKP+L+SE+P VDGKLS  CYL ALD+CY     K  K +     +   D +
Sbjct: 130 HMAHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKHLCNKFEKIEGKEFSINDADYI 189

Query: 183 LFHSPYCKLVQKSLARVCFNDFL 251
           +FHSPY KLVQKS AR+ +NDFL
Sbjct: 190 VFHSPYNKLVQKSFARLLYNDFL 212



 Score = 58.8 bits (136), Expect = 4e-09
 Identities = 35/104 (33%), Positives = 55/104 (52%), Gaps = 2/104 (1%)
 Frame = +1

Query: 499 EEVSLFSYGSGLASTMYSINVCHDMSTGSKLEKLISSLHETVALLDKRQSVEPSKFSDIM 678
           + V +FSYGSG  +TM+S+ + +D      +   I+S+ +    L  R    P KF + M
Sbjct: 295 KRVVMFSYGSGSTATMFSLRL-NDNKPPFSISN-IASVMDVGGKLKARHEYAPEKFVETM 352

Query: 679 DVRTKNYHSAPYEPT--GSLDVLFPGTYYL*KIDDQRRRTYDRK 804
            +    Y +  +  T  G +D+L PGTYYL ++D   RR Y +K
Sbjct: 353 KLMEHRYGAKDFVTTKEGIIDLLAPGTYYLKEVDSLYRRFYGKK 396



 Score = 53.2 bits (122), Expect = 2e-07
 Identities = 31/102 (30%), Positives = 54/102 (52%)
 Frame = +2

Query: 212 SKIISKSVLQ*FSELPAEEREKQFPGLSEFSNHKLEDTYFDREVEKAFMTYSLSLFEEKT 391
           ++++    L+  S +    +EK  P    +S+  L+++Y  R++EK     +   ++ K 
Sbjct: 204 ARLLYNDFLRNASSIDEAAKEKFTP----YSSLTLDESYQSRDLEKVSQQIAKPFYDAKV 259

Query: 392 KPSLHIARNVGNMYTPSLYGGLVSYLISKSPDQLIGKKFLCF 517
           +P+  I + VGNMYT SLY    S LI K  + L GK+ + F
Sbjct: 260 QPTTLIPKEVGNMYTASLYAAFAS-LIHKKHNDLAGKRVVMF 300


>At4g38110.1 68417.m05382 expressed protein 
          Length = 475

 Score = 30.3 bits (65), Expect = 1.7
 Identities = 16/57 (28%), Positives = 26/57 (45%)
 Frame = +2

Query: 275 KQFPGLSEFSNHKLEDTYFDREVEKAFMTYSLSLFEEKTKPSLHIARNVGNMYTPSL 445
           + FP + +   H L+  + DRE   A   Y  SL  + T   LH+   V + +  +L
Sbjct: 342 RSFPDVVKGVEHTLQSLHSDRETTPANFKYKRSLENQLTSTMLHLLSLVSSCHFEAL 398


>At1g08280.1 68414.m00914 glycosyl transferase family 29 protein /
           sialyltransferase family protein contains Pfam profile:
           PF00777 sialyltransferase (Glycosyltransferase family
           29)
          Length = 398

 Score = 29.9 bits (64), Expect = 2.2
 Identities = 18/54 (33%), Positives = 28/54 (51%), Gaps = 3/54 (5%)
 Frame = +1

Query: 463 LFDKQISRSVNWE---EVSLFSYGSGLASTMYSINVCHDMSTGSKLEKLISSLH 615
           L +K+    V+W    E SLF Y SG+ + M ++ +C  +S      KL S+ H
Sbjct: 298 LEEKKAKGFVDWSKDHEGSLFHYSSGMQAVMLAVGICEKVSVFG-FGKLNSTKH 350


>At4g09900.1 68417.m01622 hydrolase, alpha/beta fold family protein
           similar to ethylene-induced esterase [Citrus sinensis]
           GI:14279437, polyneuridine aldehyde esterase [Rauvolfia
           serpentina] GI:6651393, SP|Q40708 PIR7A protein {Oryza
           sativa}; contains Pfam profile PF00561: hydrolase,
           alpha/beta fold family
          Length = 349

 Score = 28.7 bits (61), Expect = 5.1
 Identities = 18/64 (28%), Positives = 31/64 (48%), Gaps = 5/64 (7%)
 Frame = +2

Query: 365 SLSLFEEKTKPSLHIARNVGN-----MYTPSLYGGLVSYLISKSPDQLIGKKFLCFHTVQ 529
           S+S  EE +KP + + +N+       +   S  G  VSY + + P+++    F+C   V 
Sbjct: 143 SVSTLEEYSKPLIELIQNLPAEEKVILVGHSTGGACVSYALERFPEKISKAIFICATMVT 202

Query: 530 DWHR 541
           D  R
Sbjct: 203 DGQR 206


>At4g39290.1 68417.m05564 kelch repeat-containing F-box family
           protein contains F-box domain Pfam:PF00646 and Kelch
           motif Pfam:PF01344
          Length = 365

 Score = 28.3 bits (60), Expect = 6.8
 Identities = 11/46 (23%), Positives = 22/46 (47%)
 Frame = -1

Query: 831 KNFTNK*LMFPIISTPSLIIYFSKVICSWEQDI*RTSRLIWCGMII 694
           K F  + +M P      ++ Y   ++  WE+ +    +LIWC  ++
Sbjct: 284 KGFVRRSIMNPRGEGVKMVNYGGNIVLLWEECVTIKKKLIWCEEVV 329


>At1g17230.1 68414.m02099 leucine-rich repeat family protein / protein
            kinase family protein contains protein kinase domain,
            Pfam:PF00069; contains leucine-rich repeats, Pfam:PF00560
          Length = 1133

 Score = 28.3 bits (60), Expect = 6.8
 Identities = 11/28 (39%), Positives = 17/28 (60%), Gaps = 2/28 (7%)
 Frame = -1

Query: 81   VWIVFHLHKETLMINLA--CKSHMHVSC 4
            +++  H H  TL+ N    C SHM++SC
Sbjct: 1101 MYLHIHTHTHTLLCNFRTFCDSHMNISC 1128


>At2g04620.1 68415.m00470 cation efflux family protein potential
           member of the cation diffusion facilitator (CDF) family,
           or cation efflux (CE) family, see PMID:11500563
          Length = 798

 Score = 27.9 bits (59), Expect = 9.0
 Identities = 11/32 (34%), Positives = 18/32 (56%)
 Frame = +1

Query: 445 IRRSCVLFDKQISRSVNWEEVSLFSYGSGLAS 540
           +R  CVLF   +  S++W+ V  F + S + S
Sbjct: 197 VRGFCVLFAGLLLLSISWDRVDCFPFSSSVES 228


>At1g33990.1 68414.m04214 hydrolase, alpha/beta fold family protein
           similar to polyneuridine aldehyde esterase GI:6651393
           from [Rauvolfia serpentina], SP|Q40708 PIR7A protein
           {Oryza sativa}, ethylene-induced esterase [Citrus
           sinensis] GI:14279437; contains Pfam profile PF00561:
           hydrolase, alpha/beta fold family
          Length = 348

 Score = 27.9 bits (59), Expect = 9.0
 Identities = 17/66 (25%), Positives = 31/66 (46%), Gaps = 5/66 (7%)
 Frame = +2

Query: 359 TYSLSLFEEKTKPSLHIARNVGN-----MYTPSLYGGLVSYLISKSPDQLIGKKFLCFHT 523
           T ++S  EE +KP + +  N+       +   S  G  +SY + + P+++    F+C   
Sbjct: 140 TNTVSTLEEYSKPLIDLLENLPEEEKVILVGHSTGGASISYALERFPEKISKAIFVCATM 199

Query: 524 VQDWHR 541
           V D  R
Sbjct: 200 VSDGQR 205


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,753,377
Number of Sequences: 28952
Number of extensions: 359477
Number of successful extensions: 895
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 874
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 893
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1960634400
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)