Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--1357
         (752 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g19006.1 68417.m02801 26S proteasome regulatory subunit, puta...    56   3e-08
At5g45620.2 68418.m05607 26S proteasome regulatory subunit, puta...    53   2e-07
At5g45620.1 68418.m05608 26S proteasome regulatory subunit, puta...    53   2e-07
At3g13150.1 68416.m01645 pentatricopeptide (PPR) repeat-containi...    32   0.47 
At2g17790.1 68415.m02062 vacuolar protein sorting-associated pro...    28   5.8  

>At4g19006.1 68417.m02801 26S proteasome regulatory subunit,
           putative (RPN9) similar to 26S proteasome subunit p40.5
           [Homo sapiens] gi|3618343|dbj|BAA33214
          Length = 386

 Score = 55.6 bits (128), Expect = 3e-08
 Identities = 26/57 (45%), Positives = 35/57 (61%)
 Frame = +2

Query: 95  FLTKKQASEPALAADWAKLEELYNKKLWHQLTLKLQEFVKNPALQRGDNLIQLSITF 265
           +L  ++ + P L   +  L +LY KKLWHQLTLKL++F+     Q GD LIQL   F
Sbjct: 6   YLESQKNAHPELGEWYNSLADLYQKKLWHQLTLKLEQFIALSVFQAGDALIQLYNNF 62



 Score = 50.4 bits (115), Expect = 1e-06
 Identities = 46/166 (27%), Positives = 74/166 (44%), Gaps = 6/166 (3%)
 Frame = +1

Query: 256 NNFLTTFESKINPLSLVEIIAHIVEQYVNKRDAVTFLEKVETKVKMNDEAL--ALCKVLQ 429
           NNF+T FE++IN L L      +  QY  K  AV++LE V  K+K   E+        ++
Sbjct: 60  NNFITDFETRINLLKLAHFAVVVSRQYPEKEAAVSYLEGVIEKLKATKESRINEPISYIE 119

Query: 430 GQIYIEQLNDYDAAE--KIIEHXXXXXXXXXXXXQFTDDSIS*PRNITESVVR--LAVTT 597
            QI + +L   D  E  KI++                  +     +    V +       
Sbjct: 120 TQIALFKLEQGDQKECKKILDDGKSLLDSMTDIDPSVYANFFWVSSQYHKVRQEFSEFYK 179

Query: 598 AQLRYVGCADGGEALPATERADIAFRLALSGVLAPDVYDLGELLAH 735
             L Y+      ++L  + + D+AF L+LS +L  ++Y+ GELLAH
Sbjct: 180 NALLYLAYT-SVDSLSESFKLDLAFDLSLSALLGENIYNFGELLAH 224


>At5g45620.2 68418.m05607 26S proteasome regulatory subunit,
           putative (RPN9) contains similarity to 26S proteasome
           subunit p40.5 GI:3618343 from [Homo sapiens]
          Length = 350

 Score = 52.8 bits (121), Expect = 2e-07
 Identities = 25/57 (43%), Positives = 33/57 (57%)
 Frame = +2

Query: 95  FLTKKQASEPALAADWAKLEELYNKKLWHQLTLKLQEFVKNPALQRGDNLIQLSITF 265
           +L   + + P L   +  L +LY KKLWHQLTLKL++F+     Q GD LIQ    F
Sbjct: 6   YLESLKNTHPELGEWYNSLADLYQKKLWHQLTLKLEQFIALAVFQAGDALIQFYHNF 62



 Score = 46.4 bits (105), Expect = 2e-05
 Identities = 47/170 (27%), Positives = 69/170 (40%), Gaps = 10/170 (5%)
 Frame = +1

Query: 256 NNFLTTFESKINPLSLVEIIAHIVEQYVNKRDAVTFLEKV-----ETKVKMNDEALALCK 420
           +NF+T FE+KIN L L      +  QY  K  AV++LE V      TK     E +   +
Sbjct: 60  HNFITDFETKINLLKLAHFAVVVSRQYSEKEAAVSYLESVIEKLRATKEPRITEPIIYIE 119

Query: 421 VLQGQIYIEQLNDYDAAEKIIEHXXXXXXXXXXXX-----QFTDDSIS*PRNITESVVRL 585
             +    +EQ  D    +KI++                   F   S    +   E     
Sbjct: 120 TQKALFKLEQ-GDQKECKKILDDGKSSLDSMTDIDPSVYANFYWVSSQYHKCRQEFSDFY 178

Query: 586 AVTTAQLRYVGCADGGEALPATERADIAFRLALSGVLAPDVYDLGELLAH 735
                 L Y    D  E+     + D+AF L+LS +L  ++Y+ GELLAH
Sbjct: 179 KSALLYLAYTSVEDLSESF----KLDLAFDLSLSALLGENIYNFGELLAH 224


>At5g45620.1 68418.m05608 26S proteasome regulatory subunit,
           putative (RPN9) contains similarity to 26S proteasome
           subunit p40.5 GI:3618343 from [Homo sapiens]
          Length = 386

 Score = 52.8 bits (121), Expect = 2e-07
 Identities = 25/57 (43%), Positives = 33/57 (57%)
 Frame = +2

Query: 95  FLTKKQASEPALAADWAKLEELYNKKLWHQLTLKLQEFVKNPALQRGDNLIQLSITF 265
           +L   + + P L   +  L +LY KKLWHQLTLKL++F+     Q GD LIQ    F
Sbjct: 6   YLESLKNTHPELGEWYNSLADLYQKKLWHQLTLKLEQFIALAVFQAGDALIQFYHNF 62



 Score = 46.4 bits (105), Expect = 2e-05
 Identities = 47/170 (27%), Positives = 69/170 (40%), Gaps = 10/170 (5%)
 Frame = +1

Query: 256 NNFLTTFESKINPLSLVEIIAHIVEQYVNKRDAVTFLEKV-----ETKVKMNDEALALCK 420
           +NF+T FE+KIN L L      +  QY  K  AV++LE V      TK     E +   +
Sbjct: 60  HNFITDFETKINLLKLAHFAVVVSRQYSEKEAAVSYLESVIEKLRATKEPRITEPIIYIE 119

Query: 421 VLQGQIYIEQLNDYDAAEKIIEHXXXXXXXXXXXX-----QFTDDSIS*PRNITESVVRL 585
             +    +EQ  D    +KI++                   F   S    +   E     
Sbjct: 120 TQKALFKLEQ-GDQKECKKILDDGKSSLDSMTDIDPSVYANFYWVSSQYHKCRQEFSDFY 178

Query: 586 AVTTAQLRYVGCADGGEALPATERADIAFRLALSGVLAPDVYDLGELLAH 735
                 L Y    D  E+     + D+AF L+LS +L  ++Y+ GELLAH
Sbjct: 179 KSALLYLAYTSVEDLSESF----KLDLAFDLSLSALLGENIYNFGELLAH 224


>At3g13150.1 68416.m01645 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat
          Length = 551

 Score = 31.9 bits (69), Expect = 0.47
 Identities = 22/76 (28%), Positives = 44/76 (57%)
 Frame = -1

Query: 728 NNSPKSYTSGASTPERASLKAISARSVAGRASPPSAQPTYRSCAVVTASRTTDSVIFRG* 549
           ++SP S +S +S+P+ +S  + S  S +  +S P +  +  S ++ ++SR   S      
Sbjct: 455 SSSPDSSSSVSSSPDSSSSVSSSPDSYSSFSSSPDSSSSV-SSSLFSSSRENSSSPDYSN 513

Query: 548 LIESSVNWSHTVSTSS 501
            + SS+++S +VS+SS
Sbjct: 514 SVSSSLDYSGSVSSSS 529


>At2g17790.1 68415.m02062 vacuolar protein sorting-associated
           protein 35 family protein / VPS35 family protein similar
           to vacuolar protein sorting 35 [Mus musculus]
           GI:11875394; contains Pfam profile PF03635: Vacuolar
           protein sorting-associated protein 35
          Length = 830

 Score = 28.3 bits (60), Expect = 5.8
 Identities = 17/37 (45%), Positives = 21/37 (56%)
 Frame = +1

Query: 634 EALPATERADIAFRLALSGVLAPDVYDLGELLAHQFF 744
           EALP     D+AFRL L    A D  D  E +A++FF
Sbjct: 608 EALPNVPSPDLAFRLYLQCAEAADKCD-EEPIAYEFF 643


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,423,464
Number of Sequences: 28952
Number of extensions: 311106
Number of successful extensions: 763
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 735
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 762
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1672953192
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)