BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1351
(700 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 29 0.85
SPAC17A5.14 |exo2||exonuclease II Exo2 |Schizosaccharomyces pomb... 27 3.4
SPAC3C7.05c |mug191||alpha-1,6-mannanase |Schizosaccharomyces po... 27 3.4
SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2 |Schizosacch... 26 6.0
SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha... 26 6.0
>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2100
Score = 28.7 bits (61), Expect = 0.85
Identities = 16/60 (26%), Positives = 27/60 (45%)
Frame = -2
Query: 558 ISSIFFSTSMSN*SDSATPLYLLMLRHQNQNNLSPQPHNF*TDYGSFLNQHCGNELLSYV 379
+S + F+ + + D+ TP YL H + NF + YG FL + ++L V
Sbjct: 878 LSQLNFNEKLKDVKDNLTPAYLPWFSHYIVTQRVSREANFLSLYGKFLEELKSSDLYKIV 937
>SPAC17A5.14 |exo2||exonuclease II Exo2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1328
Score = 26.6 bits (56), Expect = 3.4
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = +2
Query: 326 LCYQLLY*AKQPSFDSILT*LNNSLPQCWFKNE 424
LC QL+ + P FD++ +N L C KN+
Sbjct: 16 LCSQLIENDRIPEFDNLYLDMNGILHNCTHKND 48
>SPAC3C7.05c |mug191||alpha-1,6-mannanase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 442
Score = 26.6 bits (56), Expect = 3.4
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = -1
Query: 550 YFFQHIYEQLIRFSYSIIFVNVASPKSK*SITPASQLLNRL 428
+F QH+ E L+ FSY++ +A P K + A L+ L
Sbjct: 299 FFAQHLSEGLMTFSYAMPSSALAKPAQKMVLDQADFLMKYL 339
>SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2176
Score = 25.8 bits (54), Expect = 6.0
Identities = 19/69 (27%), Positives = 35/69 (50%), Gaps = 5/69 (7%)
Frame = +3
Query: 252 IQKNISTNRIVVKEINGCD-GSFI---VNCVISYCIKQN-SPLLIVSSHNSITHYHNVGL 416
++KNI + V N D G ++ C+ ++ K +PL I SITH+ ++ L
Sbjct: 1519 LEKNIRVVGLSVSVANARDLGEWLGTSPQCIFNFSPKDRPNPLTIHLQSFSITHFPSLML 1578
Query: 417 RMNHNLFKS 443
M+ +++S
Sbjct: 1579 AMSKPIYRS 1587
>SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 4196
Score = 25.8 bits (54), Expect = 6.0
Identities = 15/66 (22%), Positives = 33/66 (50%), Gaps = 6/66 (9%)
Frame = +2
Query: 473 FW*RNINKYNGVAESDQLLIDVLKKIEEMQRNHDTVNII------FDGITHLLDLQYTLP 634
FW + + + +++ + I+ +++N D++N+I DG ++ DLQ +
Sbjct: 1467 FWKNSYFSFKSFKGRNYIVVGCQELIDAVEKNMDSLNLIKTSRHFKDGDMNITDLQSKMK 1526
Query: 635 EVNKFL 652
+ KFL
Sbjct: 1527 IIVKFL 1532
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,576,835
Number of Sequences: 5004
Number of extensions: 49615
Number of successful extensions: 125
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 120
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 125
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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