Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1287
(707 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 144 1e-35
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 80 3e-16
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 80 3e-16
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 64 1e-11
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 60 3e-10
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 46 5e-06
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 43 4e-05
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 38 0.001
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 33 0.053
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 28 1.1
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 1.5
SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |S... 27 3.5
SPCC663.15c |||conserved fungal protein|Schizosaccharomyces pomb... 27 3.5
SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 26 6.1
SPBC530.14c |dsk1||SR protein-specific kinase Dsk1|Schizosacchar... 25 8.0
SPAC607.06c |||metallopeptidase|Schizosaccharomyces pombe|chr 1|... 25 8.0
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 144 bits (349), Expect = 1e-35
Identities = 65/71 (91%), Positives = 69/71 (97%)
Frame = -3
Query: 507 MYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDE 328
MYPGIADRMQKEI ALAPS+MK+KI+APPERKYSVWIGGSILASLSTFQQMWISKQEYDE
Sbjct: 305 MYPGIADRMQKEIQALAPSSMKVKIVAPPERKYSVWIGGSILASLSTFQQMWISKQEYDE 364
Query: 327 SGPSIVHRKCF 295
SGP IV+RKCF
Sbjct: 365 SGPGIVYRKCF 375
Score = 127 bits (307), Expect = 1e-30
Identities = 58/67 (86%), Positives = 60/67 (89%)
Frame = -1
Query: 707 KSYELPDGQVITIGNERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANT 528
KSYELPDGQVITIGNERFR PEALFQPS LG+E GIHE TYNSIMKCDVDIRKDLY N
Sbjct: 238 KSYELPDGQVITIGNERFRAPEALFQPSALGLENAGIHEATYNSIMKCDVDIRKDLYGNV 297
Query: 527 VLSGGTT 507
V+SGGTT
Sbjct: 298 VMSGGTT 304
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 80.2 bits (189), Expect = 3e-16
Identities = 34/66 (51%), Positives = 45/66 (68%)
Frame = -1
Query: 704 SYELPDGQVITIGNERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTV 525
+Y+LPD Q IT+G E F PE LF P +G EA G+H + SI+ D+D+R LY+N V
Sbjct: 244 TYQLPDNQTITLGTECFSAPEVLFNPEMMGSEASGLHIQLFKSILLSDIDLRSTLYSNIV 303
Query: 524 LSGGTT 507
LSGG+T
Sbjct: 304 LSGGST 309
Score = 61.3 bits (142), Expect = 1e-10
Identities = 27/68 (39%), Positives = 47/68 (69%)
Frame = -3
Query: 498 GIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGP 319
G +R E+ A++ ++KI A PER ++ W+GGSILASLSTF+++ I+ +EY ++
Sbjct: 313 GFGERFISELRAISGKKNQVKIYASPERMHNAWLGGSILASLSTFRRLLITSEEY-KNDQ 371
Query: 318 SIVHRKCF 295
+++ R+ F
Sbjct: 372 NVIFRRRF 379
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 80.2 bits (189), Expect = 3e-16
Identities = 35/67 (52%), Positives = 48/67 (71%)
Frame = -1
Query: 707 KSYELPDGQVITIGNERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANT 528
++Y LPDG+VI +G+ER+ CPE LFQP +G E G+ E +++I DVDIRK LY
Sbjct: 238 RNYTLPDGRVIKVGSERYECPECLFQPHLVGSEQPGLSEFIFDTIQAADVDIRKYLYRAI 297
Query: 527 VLSGGTT 507
VLSGG++
Sbjct: 298 VLSGGSS 304
Score = 46.0 bits (104), Expect = 5e-06
Identities = 27/74 (36%), Positives = 44/74 (59%), Gaps = 12/74 (16%)
Frame = -3
Query: 507 MYPGIADRMQKEITAL--------APSTM---KIKIIAPPERKYSVWIGGSILAS-LSTF 364
MY G+ R++KEI L P+ + K+KI P R+++V+IGG++LA ++
Sbjct: 305 MYAGLPSRLEKEIKQLWFERVLHGDPARLPNFKVKIEDAPRRRHAVFIGGAVLADIMAQN 364
Query: 363 QQMWISKQEYDESG 322
MW+SK E++E G
Sbjct: 365 DHMWVSKAEWEEYG 378
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 64.5 bits (150), Expect = 1e-11
Identities = 32/73 (43%), Positives = 50/73 (68%), Gaps = 6/73 (8%)
Frame = -3
Query: 498 GIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSILASLSTFQQMWISKQEYDE 328
G + R+Q E++ L P + ++KI A ER Y+ W+GGSIL+SL TF Q+WIS+QEY+E
Sbjct: 361 GFSLRLQNELSKLYPGS-RLKIHASGHVVERSYASWLGGSILSSLGTFHQLWISRQEYEE 419
Query: 327 SGP---SIVHRKC 298
G +++ ++C
Sbjct: 420 HGSDRLALIEKRC 432
Score = 34.7 bits (76), Expect(2) = 5e-06
Identities = 15/32 (46%), Positives = 20/32 (62%)
Frame = -1
Query: 602 GIHETTYNSIMKCDVDIRKDLYANTVLSGGTT 507
GI + N I +CDVDIR L N ++ GGT+
Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTS 357
Score = 30.7 bits (66), Expect(2) = 5e-06
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = -1
Query: 701 YELPDGQVITIGNERFRCPEALFQP 627
+E PDG + G ER++ PE LF P
Sbjct: 264 FEFPDGSRMMFGAERYQIPEHLFVP 288
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 60.1 bits (139), Expect = 3e-10
Identities = 31/76 (40%), Positives = 40/76 (52%), Gaps = 9/76 (11%)
Frame = -1
Query: 707 KSYELPDGQVITIGNERFRCPEALFQPSFLGMEAC---------GIHETTYNSIMKCDVD 555
K +E PDG G ERFR E LF PSF + G+HE Y SI+ CD +
Sbjct: 279 KPFEFPDGVTHKFGQERFRISEILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSE 338
Query: 554 IRKDLYANTVLSGGTT 507
+R L N V++GGT+
Sbjct: 339 LRSPLLNNIVVTGGTS 354
Score = 60.1 bits (139), Expect = 3e-10
Identities = 29/64 (45%), Positives = 40/64 (62%), Gaps = 2/64 (3%)
Frame = -3
Query: 507 MYPGIADRMQKEITALAP-STMKIKIIAPPER-KYSVWIGGSILASLSTFQQMWISKQEY 334
+ PG+++R+Q E+ LA S + + +VW GGSILASL FQ +W+SKQEY
Sbjct: 355 LIPGLSERLQAEVQRLATGSRINVHTAETASATSNAVWFGGSILASLDNFQHLWVSKQEY 414
Query: 333 DESG 322
DE G
Sbjct: 415 DEVG 418
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 46.0 bits (104), Expect = 5e-06
Identities = 23/58 (39%), Positives = 30/58 (51%), Gaps = 1/58 (1%)
Frame = -1
Query: 677 ITIGNERFRCPEALFQPSFLGME-ACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTT 507
I +G ERF PE F P + + E N + +D+RK LY N VLSGG+T
Sbjct: 280 IDVGFERFLAPEIFFNPEIASSDFLTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGST 337
Score = 37.5 bits (83), Expect = 0.002
Identities = 16/46 (34%), Positives = 26/46 (56%)
Frame = -3
Query: 441 IKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 304
+ +I+ ++ +VW GGS+LA F +K +Y+E G SI R
Sbjct: 374 VNVISHKRQRNAVWFGGSLLAQTPEFGSYCHTKADYEEYGASIARR 419
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 43.2 bits (97), Expect = 4e-05
Identities = 22/57 (38%), Positives = 29/57 (50%)
Frame = -1
Query: 683 QVITIGNERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGG 513
QV+ + NERF PE LF PS + + GI E S+ +I+ L N V GG
Sbjct: 272 QVLNLSNERFMIPELLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGG 328
Score = 38.7 bits (86), Expect = 8e-04
Identities = 20/68 (29%), Positives = 35/68 (51%)
Frame = -3
Query: 504 YPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDES 325
+PG R+ E+ +LAP+ ++K+ P + W S + L + I++ EY E
Sbjct: 332 FPGFHKRLSSELRSLAPANWEVKVFEPSDPICFPWKKASHM-PLEHWNANKITRSEYSEH 390
Query: 324 GPSIVHRK 301
G +I+ RK
Sbjct: 391 GANIMTRK 398
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 38.3 bits (85), Expect = 0.001
Identities = 15/50 (30%), Positives = 31/50 (62%)
Frame = -1
Query: 662 ERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGG 513
E+ R PE +F PS +G++ GI E + + + ++ ++ L +N +++GG
Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGG 643
Score = 37.9 bits (84), Expect = 0.001
Identities = 16/64 (25%), Positives = 31/64 (48%)
Frame = -3
Query: 501 PGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESG 322
PG+ R+++E+T++ P I + W G S + F+ ++++EY E G
Sbjct: 648 PGMETRIKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKFKAAKVTREEYLEKG 707
Query: 321 PSIV 310
P +
Sbjct: 708 PEYI 711
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 32.7 bits (71), Expect = 0.053
Identities = 15/71 (21%), Positives = 32/71 (45%), Gaps = 3/71 (4%)
Frame = -3
Query: 504 YPGIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSILASLSTFQQMWISKQEY 334
+PG A +++ I + + I +I PP + ++ W G I + ++WI ++
Sbjct: 542 FPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYNRIRIVSELWIKNSDW 601
Query: 333 DESGPSIVHRK 301
G ++ K
Sbjct: 602 KMLGSRVLQYK 612
>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 412
Score = 28.3 bits (60), Expect = 1.1
Identities = 13/47 (27%), Positives = 27/47 (57%)
Frame = -1
Query: 344 NKSTTSLAPPLYTGSASKRTARRCLQQPAAAAQFRLVISVNLIVRKI 204
N+S +SL+ Y+ SK+T+ L + A+ Q +++ I+R++
Sbjct: 241 NRSGSSLSSNFYSSRLSKKTSLASLNKSRASLQHKIMSLSRNIIRRV 287
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 27.9 bits (59), Expect = 1.5
Identities = 12/37 (32%), Positives = 24/37 (64%), Gaps = 3/37 (8%)
Frame = -3
Query: 402 WIGGSILASLSTFQQM---WISKQEYDESGPSIVHRK 301
++GGSI+A S + + +++ +EY + GP+ +H K
Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522
>SPBC29A10.10c |||tRNA-splicing endonuclease positive effector
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1944
Score = 26.6 bits (56), Expect = 3.5
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = -2
Query: 325 WPLHCTQEVLLNAPRVAASSSPRPLL 248
W + QEVL P++ SS+ +P+L
Sbjct: 125 WDIFRLQEVLKTVPKIDTSSASKPIL 150
>SPCC663.15c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 657
Score = 26.6 bits (56), Expect = 3.5
Identities = 15/44 (34%), Positives = 22/44 (50%), Gaps = 3/44 (6%)
Frame = +1
Query: 457 GESCDFLLHTVGDSRVHVVPPDNTVLAYKSLRM---STSHFMME 579
GE CD+ H + V V PD +A+K+L T H ++E
Sbjct: 518 GEWCDWYRHCLKQFSVEVKTPDAVDVAHKALNTLDEETKHQVLE 561
>SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 617
Score = 25.8 bits (54), Expect = 6.1
Identities = 13/37 (35%), Positives = 20/37 (54%)
Frame = -3
Query: 477 KEITALAPSTMKIKIIAPPERKYSVWIGGSILASLST 367
KEI++ A S + + + P E+ W ILA L+T
Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248
>SPBC530.14c |dsk1||SR protein-specific kinase
Dsk1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 544
Score = 25.4 bits (53), Expect = 8.0
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = +1
Query: 421 WRSNDLYLHCRWGESCD 471
+RS ++ L CRWG S D
Sbjct: 380 YRSPEVILGCRWGASAD 396
>SPAC607.06c |||metallopeptidase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 612
Score = 25.4 bits (53), Expect = 8.0
Identities = 10/33 (30%), Positives = 16/33 (48%)
Frame = +2
Query: 344 FEIHICWKVEREARIDPPIHTEYFLSGGAMIFI 442
F H C+K+ + I T YF+ G + F+
Sbjct: 364 FFYHPCFKLPSDPTYPSDIETNYFVKGETITFV 396
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,753,428
Number of Sequences: 5004
Number of extensions: 57003
Number of successful extensions: 194
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 188
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 329179816
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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