BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1281X
(403 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1198.07c |||mannan endo-1,6-alpha-mannosidase |Schizosacchar... 32 0.038
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 28 0.47
SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3 ... 27 1.4
SPBC582.04c |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 25 4.4
SPCC576.01c ||SPCPB1C11.04c|sulfonate dioxygenase |Schizosacchar... 25 4.4
SPBC4C3.06 |||actin cytoskeletal protein Syp1|Schizosaccharomyce... 25 4.4
SPAPB1A10.09 |ase1||microtubule-associated protein Ase1 |Schizos... 25 5.8
>SPBC1198.07c |||mannan endo-1,6-alpha-mannosidase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 507
Score = 31.9 bits (69), Expect = 0.038
Identities = 12/27 (44%), Positives = 19/27 (70%)
Frame = -2
Query: 93 NITTLKQGYFTSFYHGTIHH*LFIHPQ 13
N TT+ + YF++F+ G IH LF +P+
Sbjct: 31 NNTTISKPYFSTFFEGGIHSLLFFYPK 57
>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1576
Score = 28.3 bits (60), Expect = 0.47
Identities = 14/38 (36%), Positives = 26/38 (68%), Gaps = 2/38 (5%)
Frame = -3
Query: 368 VSHAAFLASLIAFLRVSASWRRLSSSFM--INTRVTHS 261
++HA L+S++ +L+VS RR+++ + IN R+T S
Sbjct: 1120 INHADILSSILDYLQVSKDKRRMATHILGQINQRLTLS 1157
>SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 932
Score = 26.6 bits (56), Expect = 1.4
Identities = 9/19 (47%), Positives = 15/19 (78%)
Frame = -2
Query: 234 APRTYNTQRTDPSVSNVPS 178
+P Y+ RT+P+V+N+PS
Sbjct: 393 SPEGYDNNRTNPTVNNLPS 411
>SPBC582.04c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 601
Score = 25.0 bits (52), Expect = 4.4
Identities = 14/46 (30%), Positives = 25/46 (54%), Gaps = 1/46 (2%)
Frame = -3
Query: 161 FSGDFYITIKIG*PLFGTFCIFSISPH*NK-DISQVFITEQSTISF 27
FSG ++ +K+G PLF + + N +I+Q F+ Q+ +F
Sbjct: 308 FSGTVFMRVKVGIPLFERLKVGTNKVKNNSVNIAQSFLEPQTRSTF 353
>SPCC576.01c ||SPCPB1C11.04c|sulfonate dioxygenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 413
Score = 25.0 bits (52), Expect = 4.4
Identities = 11/31 (35%), Positives = 19/31 (61%), Gaps = 1/31 (3%)
Frame = -1
Query: 124 NHFLAHFVSFQYHHTKTRIFHKF-LSRNNPP 35
N + H ++ Y +TRIFH+ L+ ++PP
Sbjct: 372 NRGVTHCITGAYRDDQTRIFHQCNLAASHPP 402
>SPBC4C3.06 |||actin cytoskeletal protein Syp1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 818
Score = 25.0 bits (52), Expect = 4.4
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = -3
Query: 362 HAAFLASLIAFLRVSASWRRLSSSFMINTRV 270
H +S+IAF++ + WR L SS I V
Sbjct: 663 HDESSSSMIAFVKPNPVWRNLGSSLHIEKLV 693
>SPAPB1A10.09 |ase1||microtubule-associated protein Ase1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 731
Score = 24.6 bits (51), Expect = 5.8
Identities = 11/24 (45%), Positives = 12/24 (50%)
Frame = -2
Query: 252 TDDRQPAPRTYNTQRTDPSVSNVP 181
T R P PR NTQ S+S P
Sbjct: 517 TSPRTPLPRVKNTQNPSRSISAEP 540
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,594,860
Number of Sequences: 5004
Number of extensions: 29170
Number of successful extensions: 75
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 71
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 74
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 136158338
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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