Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1278
(609 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g21540.1 68417.m03112 diacylglycerol kinase family protein co... 32 0.26
At2g04350.2 68415.m00434 long-chain-fatty-acid--CoA ligase famil... 32 0.26
At2g04350.1 68415.m00433 long-chain-fatty-acid--CoA ligase famil... 32 0.26
At5g14210.1 68418.m01660 leucine-rich repeat transmembrane prote... 29 1.8
At2g06645.1 68415.m00740 expressed protein 29 1.8
At4g12275.1 68417.m01945 reverse transcriptase-related low simil... 29 2.4
At4g35280.1 68417.m05013 zinc finger (C2H2 type) family protein ... 29 3.2
At1g67120.1 68414.m07636 midasin-related similar to Midasin (MID... 29 3.2
At3g46220.1 68416.m05003 expressed protein 28 4.2
At2g32800.1 68415.m04015 protein kinase family protein contains ... 28 4.2
At5g35604.1 68418.m04242 hypothetical protein 28 5.6
At2g25410.1 68415.m03043 hypothetical protein 27 7.4
At1g22280.2 68414.m02785 protein phosphatase 2C, putative / PP2C... 27 7.4
At1g22280.1 68414.m02786 protein phosphatase 2C, putative / PP2C... 27 7.4
At5g41100.2 68418.m04997 expressed protein 27 9.7
At5g41100.1 68418.m04996 expressed protein 27 9.7
At5g04710.1 68418.m00480 aspartyl aminopeptidase, putative simil... 27 9.7
At3g47150.1 68416.m05119 F-box family protein various predicted ... 27 9.7
>At4g21540.1 68417.m03112 diacylglycerol kinase family protein
contains INTERPRO domain, IPR001206, DAG-kinase
catalytic domain
Length = 1240
Score = 32.3 bits (70), Expect = 0.26
Identities = 19/57 (33%), Positives = 27/57 (47%)
Frame = +3
Query: 294 ISVGTGSTPVSSMLLSGGKLWCATHASIKIINPHTLQVDETFQISSETKPISHMAVA 464
+ GTG+ + S+L + G CA A+I II H VD TK S + +A
Sbjct: 471 VPAGTGNGMIKSLLDTVGLRCCANSATISIIRGHKRSVDVATIAQGNTKFFSVLMLA 527
>At2g04350.2 68415.m00434 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
(LACS8) similar to LACS 4 [SP|O35547] from Rattus
norvegicus, LACS 4 [SP|O60488] from Homo sapiens;
contains Pfam HMM hit: AMP-binding enzymes PF00501
Length = 720
Score = 32.3 bits (70), Expect = 0.26
Identities = 26/77 (33%), Positives = 37/77 (48%), Gaps = 1/77 (1%)
Frame = +1
Query: 85 TMWLGTED-GCIHVYNCMDNIRIKKNKVKLQHNSGVHSIVFVEGKVFVALGNGDLVVYVG 261
T W T D G H C++ I KK+ VKLQH V GKV ALG+ + V +
Sbjct: 558 TRWFYTGDIGRFHPDGCLEVIDRKKDIVKLQHGEYVSL-----GKVEAALGSSNYVDNIM 612
Query: 262 TLMDLGLNVALSALVPA 312
D + ++ +VP+
Sbjct: 613 VHADPINSYCVALVVPS 629
>At2g04350.1 68415.m00433 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
(LACS8) similar to LACS 4 [SP|O35547] from Rattus
norvegicus, LACS 4 [SP|O60488] from Homo sapiens;
contains Pfam HMM hit: AMP-binding enzymes PF00501
Length = 720
Score = 32.3 bits (70), Expect = 0.26
Identities = 26/77 (33%), Positives = 37/77 (48%), Gaps = 1/77 (1%)
Frame = +1
Query: 85 TMWLGTED-GCIHVYNCMDNIRIKKNKVKLQHNSGVHSIVFVEGKVFVALGNGDLVVYVG 261
T W T D G H C++ I KK+ VKLQH V GKV ALG+ + V +
Sbjct: 558 TRWFYTGDIGRFHPDGCLEVIDRKKDIVKLQHGEYVSL-----GKVEAALGSSNYVDNIM 612
Query: 262 TLMDLGLNVALSALVPA 312
D + ++ +VP+
Sbjct: 613 VHADPINSYCVALVVPS 629
>At5g14210.1 68418.m01660 leucine-rich repeat transmembrane protein
kinase, putative
Length = 812
Score = 29.5 bits (63), Expect = 1.8
Identities = 27/104 (25%), Positives = 45/104 (43%), Gaps = 1/104 (0%)
Frame = +1
Query: 172 QHNSGVHSIVFVEGKVFVALGNGDLVVYVG-TLMDLGLNVALSALVPAALR*AACCSREG 348
QH + EGK F G L+ +G ++ L V + L+ R ++CCSRE
Sbjct: 373 QHQEFLCEEAETEGKQFQGRKVGILIAVIGGAVLVLVFFVLVILLLLCTNRCSSCCSREK 432
Query: 349 SFGARPTPRSRSSILTHYRWTKHSK*VQKRSQLATWRSRASTYG 480
S P++R ++T T S V ++L + ++ G
Sbjct: 433 S-----VPQTRLKVVTDNSHTSLSSEVLASARLISQTAKLGAQG 471
>At2g06645.1 68415.m00740 expressed protein
Length = 163
Score = 29.5 bits (63), Expect = 1.8
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +3
Query: 210 GEGVCGTRKW*SRGVCRDIDGSWTERST 293
GE V G +W RG+ RD WTE ++
Sbjct: 125 GEAVDGAEEWLVRGIRRDDKALWTEHAS 152
>At4g12275.1 68417.m01945 reverse transcriptase-related low
similarity to reverse transcriptase [Arabidopsis
thaliana] GI:976278
Length = 396
Score = 29.1 bits (62), Expect = 2.4
Identities = 11/30 (36%), Positives = 19/30 (63%)
Frame = +3
Query: 243 SRGVCRDIDGSWTERSTISVGTGSTPVSSM 332
+RGV RD +GSW +++G S P++ +
Sbjct: 253 ARGVVRDGEGSWLGGFALNIGVCSAPLAEL 282
>At4g35280.1 68417.m05013 zinc finger (C2H2 type) family protein
contains Pfam domain, PF00096: Zinc finger, C2H2 type
Length = 284
Score = 28.7 bits (61), Expect = 3.2
Identities = 18/50 (36%), Positives = 26/50 (52%), Gaps = 1/50 (2%)
Frame = -2
Query: 350 LPSREQHAAHRSAAGTNADSATFSPRSINVPTYTTRSPFPSATN-TFPST 204
L S H+ + T A S++ SP+S + P Y T+ P P+A T P T
Sbjct: 32 LSSSYDHSPKKKRTKTVASSSSSSPKSASKPKY-TKKPDPNAPKITRPCT 80
>At1g67120.1 68414.m07636 midasin-related similar to Midasin
(MIDAS-containing protein) (Swiss-Prot:Q12019)
[Saccharomyces cerevisiae]; similar to Midasin
(MIDAS-containing protein) (Swiss-Prot:Q9NU22) [Homo
sapiens]; contains Prosite PS00017: ATP/GTP-binding site
motif A (P-loop)
Length = 5336
Score = 28.7 bits (61), Expect = 3.2
Identities = 15/37 (40%), Positives = 20/37 (54%)
Frame = +1
Query: 142 IRIKKNKVKLQHNSGVHSIVFVEGKVFVALGNGDLVV 252
+R K NK++ Q +SG FVEG AL G V+
Sbjct: 807 LRTKVNKIRQQIHSGGMVFTFVEGAFVTALREGHWVL 843
>At3g46220.1 68416.m05003 expressed protein
Length = 530
Score = 28.3 bits (60), Expect = 4.2
Identities = 15/48 (31%), Positives = 30/48 (62%)
Frame = +3
Query: 393 HTLQVDETFQISSETKPISHMAVAGVNIWLSLHNAAQLRATIRTLASS 536
H+ + D Q+S+E+ PI+ +A +++ +HN A L+A R +A++
Sbjct: 412 HSYRKDLISQVSTESDPIALLAKVVSLLFIKIHNKA-LQAPGRAIAAA 458
>At2g32800.1 68415.m04015 protein kinase family protein contains
dual protein kinase domains, Pfam:PF00069
Length = 851
Score = 28.3 bits (60), Expect = 4.2
Identities = 16/57 (28%), Positives = 30/57 (52%), Gaps = 2/57 (3%)
Frame = -2
Query: 365 GRAPKLPSREQHAAH--RSAAGTNADSATFSPRSINVPTYTTRSPFPSATNTFPSTN 201
G P LPS + H + S+ + + SAT + + T T+ + F +++ + PS+N
Sbjct: 422 GNLPALPSFKSHPLYIPLSSLKSTSTSATTTTTRTTMTTTTSTTSFNASSESTPSSN 478
>At5g35604.1 68418.m04242 hypothetical protein
Length = 298
Score = 27.9 bits (59), Expect = 5.6
Identities = 18/49 (36%), Positives = 25/49 (51%)
Frame = -2
Query: 365 GRAPKLPSREQHAAHRSAAGTNADSATFSPRSINVPTYTTRSPFPSATN 219
GR+P+ PSR + R A D SPR ++ P+ T P P AT+
Sbjct: 68 GRSPRRPSRGNSSPRRDKARARTDC---SPR-LSPPSRTMGPPPPVATS 112
>At2g25410.1 68415.m03043 hypothetical protein
Length = 377
Score = 27.5 bits (58), Expect = 7.4
Identities = 24/90 (26%), Positives = 41/90 (45%), Gaps = 5/90 (5%)
Frame = -3
Query: 274 DPSMSRHTPRDHHFLVPQTP-SPPRILWNEPLNCVAT*LYSF*F*YCPCNCIRGCNHP-- 104
D + + P+ FLV + RI N+P NC+A L +F P + +R N+
Sbjct: 67 DANTTLKLPKSEPFLVKEIDYETQRIRLNDPENCLARRLLNFDPSGSPFSFLRSRNYTFL 126
Query: 103 QCP--ATLWAG*QLLHCLLKTDDWLEMIEY 20
CP A + A + + CL T ++++
Sbjct: 127 ICPKEANITASFRAIDCLGNTTSSFFVVQF 156
>At1g22280.2 68414.m02785 protein phosphatase 2C, putative / PP2C,
putative similar to protein phosphatase type 2C
GI:4336436 from [Lotus japonicus]
Length = 199
Score = 27.5 bits (58), Expect = 7.4
Identities = 11/29 (37%), Positives = 18/29 (62%)
Frame = +3
Query: 309 GSTPVSSMLLSGGKLWCATHASIKIINPH 395
GST V+++L++G KLW A + + H
Sbjct: 125 GSTAVTAILINGRKLWIANVGDSRAVLSH 153
>At1g22280.1 68414.m02786 protein phosphatase 2C, putative / PP2C,
putative similar to protein phosphatase type 2C
GI:4336436 from [Lotus japonicus]
Length = 281
Score = 27.5 bits (58), Expect = 7.4
Identities = 11/29 (37%), Positives = 18/29 (62%)
Frame = +3
Query: 309 GSTPVSSMLLSGGKLWCATHASIKIINPH 395
GST V+++L++G KLW A + + H
Sbjct: 125 GSTAVTAILINGRKLWIANVGDSRAVLSH 153
>At5g41100.2 68418.m04997 expressed protein
Length = 582
Score = 27.1 bits (57), Expect = 9.7
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = -3
Query: 376 IEAWVAHQSFPPESNMLLTGVLPVPTLIVLRSVQDPSMSR 257
+ AW +S S ++ LPVP L+V RS PS ++
Sbjct: 486 LTAWNQERSNVVVSTNIVASPLPVPPLVVPRSYSIPSRNQ 525
>At5g41100.1 68418.m04996 expressed protein
Length = 586
Score = 27.1 bits (57), Expect = 9.7
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = -3
Query: 376 IEAWVAHQSFPPESNMLLTGVLPVPTLIVLRSVQDPSMSR 257
+ AW +S S ++ LPVP L+V RS PS ++
Sbjct: 486 LTAWNQERSNVVVSTNIVASPLPVPPLVVPRSYSIPSRNQ 525
>At5g04710.1 68418.m00480 aspartyl aminopeptidase, putative similar
to SP|Q9ULA0 Aspartyl aminopeptidase (EC 3.4.11.21)
{Homo sapiens}; contains Pfam profile PF02127:
Aminopeptidase I zinc metalloprotease (M18)
Length = 526
Score = 27.1 bits (57), Expect = 9.7
Identities = 11/27 (40%), Positives = 18/27 (66%)
Frame = -2
Query: 290 ATFSPRSINVPTYTTRSPFPSATNTFP 210
++F +S++ PTY RSPF S ++ P
Sbjct: 20 SSFLSQSLSFPTYLHRSPFRSFSSVSP 46
>At3g47150.1 68416.m05119 F-box family protein various predicted
proteins, Arabidopsis thaliana
Length = 360
Score = 27.1 bits (57), Expect = 9.7
Identities = 17/49 (34%), Positives = 27/49 (55%), Gaps = 3/49 (6%)
Frame = -3
Query: 334 NMLLTGVLPVPTLIVLRSVQD---PSMSRHTPRDHHFLVPQTPSPPRIL 197
N+LL LPV +L+ R V ++ H R+ HF + + +PPR+L
Sbjct: 19 NILLR--LPVKSLLRFRCVSKLWCSIITSHDFRNRHFNITSSSAPPRLL 65
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,906,604
Number of Sequences: 28952
Number of extensions: 346640
Number of successful extensions: 1149
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1117
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1149
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1216725696
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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