Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1262
(819 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g35390.2 68415.m04339 ribose-phosphate pyrophosphokinase 1 / ... 59 3e-09
At2g35390.1 68415.m04338 ribose-phosphate pyrophosphokinase 1 / ... 59 3e-09
At2g44530.1 68415.m05539 ribose-phosphate pyrophosphokinase, put... 59 4e-09
At1g32380.1 68414.m03995 ribose-phosphate pyrophosphokinase 2 / ... 59 4e-09
At1g07390.1 68414.m00788 leucine-rich repeat family protein cont... 28 6.5
At5g35160.1 68418.m04167 endomembrane protein 70, putative p76, ... 28 8.6
At2g40140.1 68415.m04937 zinc finger (CCCH-type) family protein ... 28 8.6
>At2g35390.2 68415.m04339 ribose-phosphate pyrophosphokinase 1 /
phosphoribosyl diphosphate synthetase 1 (PRSI) identical
to phosphoribosyl diphosphate synthetase 1
(ribose-phosphate pyrophosphokinase 1 (PRS I)
[Arabidopsis thaliana] GI:633140, SP|Q42581
Length = 403
Score = 59.3 bits (137), Expect = 3e-09
Identities = 25/69 (36%), Positives = 42/69 (60%)
Frame = +3
Query: 297 GGCSVYHKTNRETIVEIADSIRGKNIYIIQTGTKDVNNNIMELLIMAYACKTSSASSIVG 476
G ++ + E V++ +S+RG ++Y++Q N N+MELLIM AC+ +SA +
Sbjct: 117 GKVNIKRFADGEIYVQLQESVRGCDVYLVQPTCTPTNENLMELLIMVDACRRASAKKVTA 176
Query: 477 VIPYLPYSK 503
VIPY Y++
Sbjct: 177 VIPYFGYAR 185
Score = 51.6 bits (118), Expect = 6e-07
Identities = 30/90 (33%), Positives = 48/90 (53%), Gaps = 7/90 (7%)
Frame = +2
Query: 509 KMRKRGCIVSKLLAQMMCKSGLSHIITMDLHQKEIQGFFDCPVDNLRASPFLLQYI-PES 685
K + R I +KL+A ++ ++G ++ DLH + G+FD PVD++ P +L Y+ +S
Sbjct: 189 KTQGRESIAAKLVANLITEAGADRVLACDLHSGQSMGYFDIPVDHVYCQPVILDYLASKS 248
Query: 686 IPDYRNSVI------VARNPALQRKLLHMP 757
IP V+ VAR A +KL P
Sbjct: 249 IPSEDLVVVSPDVGGVARARAFAKKLSDAP 278
>At2g35390.1 68415.m04338 ribose-phosphate pyrophosphokinase 1 /
phosphoribosyl diphosphate synthetase 1 (PRSI) identical
to phosphoribosyl diphosphate synthetase 1
(ribose-phosphate pyrophosphokinase 1 (PRS I)
[Arabidopsis thaliana] GI:633140, SP|Q42581
Length = 352
Score = 59.3 bits (137), Expect = 3e-09
Identities = 25/69 (36%), Positives = 42/69 (60%)
Frame = +3
Query: 297 GGCSVYHKTNRETIVEIADSIRGKNIYIIQTGTKDVNNNIMELLIMAYACKTSSASSIVG 476
G ++ + E V++ +S+RG ++Y++Q N N+MELLIM AC+ +SA +
Sbjct: 66 GKVNIKRFADGEIYVQLQESVRGCDVYLVQPTCTPTNENLMELLIMVDACRRASAKKVTA 125
Query: 477 VIPYLPYSK 503
VIPY Y++
Sbjct: 126 VIPYFGYAR 134
Score = 51.6 bits (118), Expect = 6e-07
Identities = 30/90 (33%), Positives = 48/90 (53%), Gaps = 7/90 (7%)
Frame = +2
Query: 509 KMRKRGCIVSKLLAQMMCKSGLSHIITMDLHQKEIQGFFDCPVDNLRASPFLLQYI-PES 685
K + R I +KL+A ++ ++G ++ DLH + G+FD PVD++ P +L Y+ +S
Sbjct: 138 KTQGRESIAAKLVANLITEAGADRVLACDLHSGQSMGYFDIPVDHVYCQPVILDYLASKS 197
Query: 686 IPDYRNSVI------VARNPALQRKLLHMP 757
IP V+ VAR A +KL P
Sbjct: 198 IPSEDLVVVSPDVGGVARARAFAKKLSDAP 227
>At2g44530.1 68415.m05539 ribose-phosphate pyrophosphokinase,
putative / phosphoribosyl diphosphate synthetase,
putative very strong similarity to phosphoribosyl
pyrophosphate synthase [Spinacia oleracea] GI:4902849;
contains Pfam profile PF00156: Phosphoribosyl
transferase domain
Length = 394
Score = 58.8 bits (136), Expect = 4e-09
Identities = 37/124 (29%), Positives = 61/124 (49%)
Frame = +3
Query: 132 GFRLLCYSTKPGWWVYRIDLKIAIWTGTPRRTLLF*VETHI*IS*FDCQPPRVRKGGCSV 311
GF LL T P + R L+ +I+ T R + I C + G +
Sbjct: 49 GFHLLTGDTVPASFS-RTRLEDSIYQNTTRLRIFSGTANPILAQEISCYLG-LDLGKIKI 106
Query: 312 YHKTNRETIVEIADSIRGKNIYIIQTGTKDVNNNIMELLIMAYACKTSSASSIVGVIPYL 491
+ E V++ +S+RG +++++Q N N+MELL+M AC+ +SA +I VIPY
Sbjct: 107 KRFADGEIYVQLQESVRGCDVFLVQPTCPPANENLMELLVMIDACRRASAKTITAVIPYF 166
Query: 492 PYSK 503
Y++
Sbjct: 167 GYAR 170
Score = 51.6 bits (118), Expect = 6e-07
Identities = 22/70 (31%), Positives = 39/70 (55%)
Frame = +2
Query: 509 KMRKRGCIVSKLLAQMMCKSGLSHIITMDLHQKEIQGFFDCPVDNLRASPFLLQYIPESI 688
K + R I +KL+A ++ +SG ++ DLH + G+FD PVD++ P +L Y+
Sbjct: 174 KTQGRESIAAKLVANLITQSGADRVLACDLHSGQSMGYFDIPVDHVYGQPVILDYLASKA 233
Query: 689 PDYRNSVIVA 718
+ V+V+
Sbjct: 234 ISSEDLVVVS 243
>At1g32380.1 68414.m03995 ribose-phosphate pyrophosphokinase 2 /
phosphoribosyl diphosphate synthetase 2 (PRS2) identical
to SP:Q42583 from [Arabidopsis thaliana]; strong
similarity to phosphoribosyl diphosphate synthetase 1
(ribose-phosphate pyrophosphokinase 1 (PRS I)
[Arabidopsis thaliana] GI:633140, SP|Q42581
Length = 400
Score = 58.8 bits (136), Expect = 4e-09
Identities = 25/69 (36%), Positives = 42/69 (60%)
Frame = +3
Query: 297 GGCSVYHKTNRETIVEIADSIRGKNIYIIQTGTKDVNNNIMELLIMAYACKTSSASSIVG 476
G S+ + E V++ +S+RG +++++Q N N+MELLIM AC+ +SA +
Sbjct: 114 GKVSIKRFADGEIYVQLKESVRGCDVFLVQPTCTPTNENLMELLIMVDACRRASAKKVTA 173
Query: 477 VIPYLPYSK 503
VIPY Y++
Sbjct: 174 VIPYFGYAR 182
Score = 50.0 bits (114), Expect = 2e-06
Identities = 21/70 (30%), Positives = 39/70 (55%)
Frame = +2
Query: 509 KMRKRGCIVSKLLAQMMCKSGLSHIITMDLHQKEIQGFFDCPVDNLRASPFLLQYIPESI 688
K + R I +KL+A ++ ++G ++ DLH + G+FD PVD++ P +L Y+
Sbjct: 186 KTQGRESIAAKLVANLITEAGADRVLACDLHSGQSMGYFDIPVDHVYCQPVILDYLASKS 245
Query: 689 PDYRNSVIVA 718
+ V+V+
Sbjct: 246 ISSEDLVVVS 255
>At1g07390.1 68414.m00788 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; contains similarity to Hcr2-5D
[Lycopersicon esculentum] gi|3894393|gb|AAC78596
Length = 976
Score = 28.3 bits (60), Expect = 6.5
Identities = 16/57 (28%), Positives = 28/57 (49%)
Frame = +2
Query: 506 MKMRKRGCIVSKLLAQMMCKSGLSHIITMDLHQKEIQGFFDCPVDNLRASPFLLQYI 676
MK+R+ + L + C L+H+ T+DL ++ G V L P +L+Y+
Sbjct: 332 MKLRELDLSSNALTSLPYCLGNLTHLRTLDLSNNQLNGNLSSFVSGL---PSVLEYL 385
>At5g35160.1 68418.m04167 endomembrane protein 70, putative p76,
Homo sapiens, EMBL:HSU81006
Length = 627
Score = 27.9 bits (59), Expect = 8.6
Identities = 13/45 (28%), Positives = 22/45 (48%)
Frame = -3
Query: 343 STMVSLLVLWYTEQPPLRTLGGWQSNQLIQM*VSTQNNNVRRGVP 209
S V LL+LW+ PL +GG+ + + + N + R +P
Sbjct: 433 SLFVILLLLWFCISVPLTLIGGYFGAKAPHIEFPVRTNQIPREIP 477
>At2g40140.1 68415.m04937 zinc finger (CCCH-type) family protein
contains Pfam domain, PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar) and Pfam domain,
PF00023: Ankyrin repeat
Length = 597
Score = 27.9 bits (59), Expect = 8.6
Identities = 17/40 (42%), Positives = 20/40 (50%), Gaps = 1/40 (2%)
Frame = +3
Query: 375 YIIQTGTKDVNNNIMELLIMAYACKTSSAS-SIVGVIPYL 491
YII TG DVN + + A C S S SIV +I L
Sbjct: 95 YIIATGRSDVNRVCSDEKVTALHCAVSGCSVSIVEIIKIL 134
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,045,316
Number of Sequences: 28952
Number of extensions: 386679
Number of successful extensions: 905
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 881
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 905
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1872844800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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