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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--1253
         (706 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g20850.1 68418.m02476 DNA repair protein RAD51, putative iden...    58   4e-09
At3g22880.1 68416.m02884 meiotic recombination protein, putative...    43   2e-04
At1g65890.1 68414.m07477 acyl-activating enzyme 12 (AAE12) simil...    31   0.98 
At1g29900.1 68414.m03654 carbamoyl-phosphate synthase family pro...    29   2.3  
At5g41580.1 68418.m05052 zinc finger (MIZ type) family protein c...    28   5.2  

>At5g20850.1 68418.m02476 DNA repair protein RAD51, putative
           identical to Rad51-like protein [Arabidopsis thaliana]
           GI:2388778; strong similarity to SP|Q06609 DNA repair
           protein RAD51 homolog 1 {Homo sapiens}; contains Pfam
           profile PF00633: Helix-hairpin-helix motif
          Length = 342

 Score = 58.4 bits (135), Expect = 4e-09
 Identities = 35/93 (37%), Positives = 48/93 (51%), Gaps = 1/93 (1%)
 Frame = +1

Query: 379 KKWLITIKGISEAKADKILAEASKLVPMGFTTATEFHQKRAEINNLLLVQRN*IDYWEXX 558
           +K L+ IKGIS+AK DKI+  ASKLVP+GFT+A++ H +R EI  +    R      E  
Sbjct: 60  RKDLLQIKGISDAKVDKIVEAASKLVPLGFTSASQLHAQRQEIIQITSGSRELDKVLEGG 119

Query: 559 XXXXXXXXXXXXXXXA-NPIMSPLAVTCQLPLN 654
                              +   L VTCQLP++
Sbjct: 120 IETGSITELYGEFRSGKTQLCHTLCVTCQLPMD 152



 Score = 40.7 bits (91), Expect = 0.001
 Identities = 19/34 (55%), Positives = 23/34 (67%)
 Frame = +2

Query: 305 GITSGDIKKLEEAGYHTVESVAYAPKNG*SQLKG 406
           GI S D+KKL +AG  TVE VAY P+    Q+KG
Sbjct: 35  GIASVDVKKLRDAGLCTVEGVAYTPRKDLLQIKG 68



 Score = 34.7 bits (76), Expect = 0.060
 Identities = 20/57 (35%), Positives = 28/57 (49%), Gaps = 1/57 (1%)
 Frame = +3

Query: 510 QLTTGSKELDRLLXXXXXXXXXXXXXXXFRTGKPNYV-TLSSHLPATIEQSGGEGNA 677
           Q+T+GS+ELD++L               FR+GK     TL       ++Q GGEG A
Sbjct: 104 QITSGSRELDKVLEGGIETGSITELYGEFRSGKTQLCHTLCVTCQLPMDQGGGEGKA 160


>At3g22880.1 68416.m02884 meiotic recombination protein, putative
           similar to Swiss-Prot:Q14565 meiotic recombination
           protein DMC1/LIM15 homolog [Homo sapiens]; contains
           non-consensus AT/AC non-consensus splice sites at intron
           14
          Length = 344

 Score = 43.2 bits (97), Expect = 2e-04
 Identities = 25/61 (40%), Positives = 34/61 (55%)
 Frame = +1

Query: 325 KKA*GSGISYC*ICCLCSKKWLITIKGISEAKADKILAEASKLVPMGFTTATEFHQKRAE 504
           KK   +GI  C    + +KK L  IKG+SEAK DKI   A K+V  G+ T ++   KR  
Sbjct: 45  KKLQEAGIHTCNGLMMHTKKNLTGIKGLSEAKVDKICEAAEKIVNFGYMTGSDALIKRKS 104

Query: 505 I 507
           +
Sbjct: 105 V 105



 Score = 35.5 bits (78), Expect = 0.035
 Identities = 16/39 (41%), Positives = 22/39 (56%)
 Frame = +2

Query: 290 KRSGNGITSGDIKKLEEAGYHTVESVAYAPKNG*SQLKG 406
           K    GI +GD+KKL+EAG HT   +    K   + +KG
Sbjct: 33  KLIAQGINAGDVKKLQEAGIHTCNGLMMHTKKNLTGIKG 71


>At1g65890.1 68414.m07477 acyl-activating enzyme 12 (AAE12) similar
           to AMP-binding protein GI:1903034 from [Brassica napus];
           contains Pfam AMP-binding domain PF00501; identical to
           cDNA acyl-activating enzyme 12 (At1g65890) mRNA
           GI:29893228, acyl-activating enzyme 12 [Arabidopsis
           thaliana] GI:29893229
          Length = 578

 Score = 30.7 bits (66), Expect = 0.98
 Identities = 20/63 (31%), Positives = 34/63 (53%), Gaps = 2/63 (3%)
 Frame = +2

Query: 251 LPKNFQIDLQKTQKRSGNGITSGDI--KKLEEAGYHTVESVAYAPKNG*SQLKGYLKRRQ 424
           LP+N Q++L+  Q  S  G+T  D+  K+ +E+     +++      G S +KGYLK  +
Sbjct: 352 LPENQQMELKARQGLSILGLTEVDVRNKETQESVPRDGKTMGEIVMKGSSIMKGYLKNPK 411

Query: 425 IKY 433
             Y
Sbjct: 412 ATY 414


>At1g29900.1 68414.m03654 carbamoyl-phosphate synthase family
           protein similar to carbamoylphosphate synthetase
           GI:6552726 from [Medicago sativa]; contains Pfam
           profiles PF02786: Carbamoyl-phosphate synthase L chain
           ATP binding domain, PF00289: Carbamoyl-phosphate
           synthase L chain N-terminal domain, PF02787:
           Carbamoyl-phosphate synthetase large chain
           oligomerisation domain
          Length = 1187

 Score = 29.5 bits (63), Expect = 2.3
 Identities = 13/44 (29%), Positives = 22/44 (50%)
 Frame = +2

Query: 248 YLPKNFQIDLQKTQKRSGNGITSGDIKKLEEAGYHTVESVAYAP 379
           YL    +ID+       GN +  G ++ +E+AG H+ +S    P
Sbjct: 858 YLSDAIEIDVDTLTDSYGNVVIGGIMEHIEQAGVHSGDSACMLP 901


>At5g41580.1 68418.m05052 zinc finger (MIZ type) family protein
           contains Pfam domain PF02891: MIZ zinc finger
          Length = 703

 Score = 28.3 bits (60), Expect = 5.2
 Identities = 16/56 (28%), Positives = 31/56 (55%), Gaps = 1/56 (1%)
 Frame = +2

Query: 176 SKISTFQIKDLSRKEKTRVKEGQVYLPKNFQIDLQKTQKRSGNGITSGDI-KKLEE 340
           S +++F++  ++++ +  +++G    PK FQI      K     I + DI KK+EE
Sbjct: 22  SLVNSFRLASVTQRLRYHIQDGAKVDPKEFQICCISFAKGIDFAIANNDIPKKVEE 77


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,739,573
Number of Sequences: 28952
Number of extensions: 262110
Number of successful extensions: 610
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 592
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 609
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1516419560
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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