BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--1253 (706 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g20850.1 68418.m02476 DNA repair protein RAD51, putative iden... 58 4e-09 At3g22880.1 68416.m02884 meiotic recombination protein, putative... 43 2e-04 At1g65890.1 68414.m07477 acyl-activating enzyme 12 (AAE12) simil... 31 0.98 At1g29900.1 68414.m03654 carbamoyl-phosphate synthase family pro... 29 2.3 At5g41580.1 68418.m05052 zinc finger (MIZ type) family protein c... 28 5.2 >At5g20850.1 68418.m02476 DNA repair protein RAD51, putative identical to Rad51-like protein [Arabidopsis thaliana] GI:2388778; strong similarity to SP|Q06609 DNA repair protein RAD51 homolog 1 {Homo sapiens}; contains Pfam profile PF00633: Helix-hairpin-helix motif Length = 342 Score = 58.4 bits (135), Expect = 4e-09 Identities = 35/93 (37%), Positives = 48/93 (51%), Gaps = 1/93 (1%) Frame = +1 Query: 379 KKWLITIKGISEAKADKILAEASKLVPMGFTTATEFHQKRAEINNLLLVQRN*IDYWEXX 558 +K L+ IKGIS+AK DKI+ ASKLVP+GFT+A++ H +R EI + R E Sbjct: 60 RKDLLQIKGISDAKVDKIVEAASKLVPLGFTSASQLHAQRQEIIQITSGSRELDKVLEGG 119 Query: 559 XXXXXXXXXXXXXXXA-NPIMSPLAVTCQLPLN 654 + L VTCQLP++ Sbjct: 120 IETGSITELYGEFRSGKTQLCHTLCVTCQLPMD 152 Score = 40.7 bits (91), Expect = 0.001 Identities = 19/34 (55%), Positives = 23/34 (67%) Frame = +2 Query: 305 GITSGDIKKLEEAGYHTVESVAYAPKNG*SQLKG 406 GI S D+KKL +AG TVE VAY P+ Q+KG Sbjct: 35 GIASVDVKKLRDAGLCTVEGVAYTPRKDLLQIKG 68 Score = 34.7 bits (76), Expect = 0.060 Identities = 20/57 (35%), Positives = 28/57 (49%), Gaps = 1/57 (1%) Frame = +3 Query: 510 QLTTGSKELDRLLXXXXXXXXXXXXXXXFRTGKPNYV-TLSSHLPATIEQSGGEGNA 677 Q+T+GS+ELD++L FR+GK TL ++Q GGEG A Sbjct: 104 QITSGSRELDKVLEGGIETGSITELYGEFRSGKTQLCHTLCVTCQLPMDQGGGEGKA 160 >At3g22880.1 68416.m02884 meiotic recombination protein, putative similar to Swiss-Prot:Q14565 meiotic recombination protein DMC1/LIM15 homolog [Homo sapiens]; contains non-consensus AT/AC non-consensus splice sites at intron 14 Length = 344 Score = 43.2 bits (97), Expect = 2e-04 Identities = 25/61 (40%), Positives = 34/61 (55%) Frame = +1 Query: 325 KKA*GSGISYC*ICCLCSKKWLITIKGISEAKADKILAEASKLVPMGFTTATEFHQKRAE 504 KK +GI C + +KK L IKG+SEAK DKI A K+V G+ T ++ KR Sbjct: 45 KKLQEAGIHTCNGLMMHTKKNLTGIKGLSEAKVDKICEAAEKIVNFGYMTGSDALIKRKS 104 Query: 505 I 507 + Sbjct: 105 V 105 Score = 35.5 bits (78), Expect = 0.035 Identities = 16/39 (41%), Positives = 22/39 (56%) Frame = +2 Query: 290 KRSGNGITSGDIKKLEEAGYHTVESVAYAPKNG*SQLKG 406 K GI +GD+KKL+EAG HT + K + +KG Sbjct: 33 KLIAQGINAGDVKKLQEAGIHTCNGLMMHTKKNLTGIKG 71 >At1g65890.1 68414.m07477 acyl-activating enzyme 12 (AAE12) similar to AMP-binding protein GI:1903034 from [Brassica napus]; contains Pfam AMP-binding domain PF00501; identical to cDNA acyl-activating enzyme 12 (At1g65890) mRNA GI:29893228, acyl-activating enzyme 12 [Arabidopsis thaliana] GI:29893229 Length = 578 Score = 30.7 bits (66), Expect = 0.98 Identities = 20/63 (31%), Positives = 34/63 (53%), Gaps = 2/63 (3%) Frame = +2 Query: 251 LPKNFQIDLQKTQKRSGNGITSGDI--KKLEEAGYHTVESVAYAPKNG*SQLKGYLKRRQ 424 LP+N Q++L+ Q S G+T D+ K+ +E+ +++ G S +KGYLK + Sbjct: 352 LPENQQMELKARQGLSILGLTEVDVRNKETQESVPRDGKTMGEIVMKGSSIMKGYLKNPK 411 Query: 425 IKY 433 Y Sbjct: 412 ATY 414 >At1g29900.1 68414.m03654 carbamoyl-phosphate synthase family protein similar to carbamoylphosphate synthetase GI:6552726 from [Medicago sativa]; contains Pfam profiles PF02786: Carbamoyl-phosphate synthase L chain ATP binding domain, PF00289: Carbamoyl-phosphate synthase L chain N-terminal domain, PF02787: Carbamoyl-phosphate synthetase large chain oligomerisation domain Length = 1187 Score = 29.5 bits (63), Expect = 2.3 Identities = 13/44 (29%), Positives = 22/44 (50%) Frame = +2 Query: 248 YLPKNFQIDLQKTQKRSGNGITSGDIKKLEEAGYHTVESVAYAP 379 YL +ID+ GN + G ++ +E+AG H+ +S P Sbjct: 858 YLSDAIEIDVDTLTDSYGNVVIGGIMEHIEQAGVHSGDSACMLP 901 >At5g41580.1 68418.m05052 zinc finger (MIZ type) family protein contains Pfam domain PF02891: MIZ zinc finger Length = 703 Score = 28.3 bits (60), Expect = 5.2 Identities = 16/56 (28%), Positives = 31/56 (55%), Gaps = 1/56 (1%) Frame = +2 Query: 176 SKISTFQIKDLSRKEKTRVKEGQVYLPKNFQIDLQKTQKRSGNGITSGDI-KKLEE 340 S +++F++ ++++ + +++G PK FQI K I + DI KK+EE Sbjct: 22 SLVNSFRLASVTQRLRYHIQDGAKVDPKEFQICCISFAKGIDFAIANNDIPKKVEE 77 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,739,573 Number of Sequences: 28952 Number of extensions: 262110 Number of successful extensions: 610 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 592 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 609 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1516419560 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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