Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1250
(774 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 154 1e-38
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 100 2e-22
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 100 5e-22
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 79 7e-16
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 29 0.98
SPBC16D10.03 |pgp2||metallopeptidase Pgp2|Schizosaccharomyces po... 28 1.3
SPCC16C4.07 |scw1||RNA-binding protein Scw1|Schizosaccharomyces ... 27 3.9
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 3.9
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 25 9.1
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 154 bits (374), Expect = 1e-38
Identities = 67/83 (80%), Positives = 75/83 (90%)
Frame = +3
Query: 261 SGGKYVPRAILLDLEPGTMDAVRSGAYGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 440
+GGKYVPRA+L+DLEPGTMDAV+SG +G LFRPDN ++GQSGAGN WAKGHYTEGAEL D
Sbjct: 55 AGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELAD 114
Query: 441 AVLDVVRKECENCDCLQGFQLTH 509
AVLDVVR+E E CD LQGFQLTH
Sbjct: 115 AVLDVVRREAEACDALQGFQLTH 137
Score = 102 bits (245), Expect = 5e-23
Identities = 50/75 (66%), Positives = 59/75 (78%), Gaps = 1/75 (1%)
Frame = +2
Query: 551 ISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNAVLSIHQLVENTDETYCIDNEAF-TIS 727
+SKIREEYPDR+M T+SV P+PK SDTVVEPYNA LS+HQLVEN+DET+CIDNEA +I
Sbjct: 152 LSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIF 211
Query: 728 ATGLSRYRTNVRDLN 772
A L + DLN
Sbjct: 212 ANTLKIKSPSYDDLN 226
Score = 91.5 bits (217), Expect = 1e-19
Identities = 36/55 (65%), Positives = 47/55 (85%)
Frame = +1
Query: 82 MREIVHLQAGQCGNQIGAKFWEIISEEHGIDPTGVYRGTSDLQLERISVYYNEAS 246
MREIVH+QAGQCGNQ+GA FW I++EHG+D G+Y GTS+ Q ER++VY+NEA+
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAA 55
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 100 bits (240), Expect = 2e-22
Identities = 45/86 (52%), Positives = 60/86 (69%), Gaps = 1/86 (1%)
Frame = +3
Query: 255 AESG-GKYVPRAILLDLEPGTMDAVRSGAYGQLFRPDNFVFGQSGAGNNWAKGHYTEGAE 431
+E+G GKYVPR+I +DLEP +D VR+G Y LF P+ + G+ A NN+A+GHYT G E
Sbjct: 54 SETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKE 113
Query: 432 LVDAVLDVVRKECENCDCLQGFQLTH 509
LVD V D +R+ +NC LQGF + H
Sbjct: 114 LVDEVTDKIRRIADNCSGLQGFLVFH 139
Score = 54.8 bits (126), Expect = 1e-08
Identities = 27/75 (36%), Positives = 45/75 (60%), Gaps = 1/75 (1%)
Frame = +2
Query: 551 ISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNAVLSIHQLVENTDETYCIDNEA-FTIS 727
+ ++ EY + +SV P+P+VS +VVEPYN+VL+ H ++ D T+ +DNE+ + I
Sbjct: 154 LERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDIC 213
Query: 728 ATGLSRYRTNVRDLN 772
L R + +LN
Sbjct: 214 RRNLDIERPSYENLN 228
Score = 44.0 bits (99), Expect = 2e-05
Identities = 18/34 (52%), Positives = 21/34 (61%)
Frame = +1
Query: 82 MREIVHLQAGQCGNQIGAKFWEIISEEHGIDPTG 183
MREI+ + GQ G QIG WE+ EHGI P G
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNG 34
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 99.5 bits (237), Expect = 5e-22
Identities = 43/86 (50%), Positives = 62/86 (72%), Gaps = 1/86 (1%)
Frame = +3
Query: 255 AESG-GKYVPRAILLDLEPGTMDAVRSGAYGQLFRPDNFVFGQSGAGNNWAKGHYTEGAE 431
+E+G GK+VPR+I +DLEP +D VR+G Y LF P+ V G+ A NN+A+GHYT G E
Sbjct: 58 SETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKE 117
Query: 432 LVDAVLDVVRKECENCDCLQGFQLTH 509
++D+VL+ +R+ +NC LQGF + H
Sbjct: 118 MIDSVLERIRRMADNCSGLQGFLVFH 143
Score = 59.3 bits (137), Expect = 6e-10
Identities = 29/75 (38%), Positives = 46/75 (61%), Gaps = 1/75 (1%)
Frame = +2
Query: 551 ISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNAVLSIHQLVENTDETYCIDNEA-FTIS 727
+ ++ EY + +SV P+P+VS +VVEPYN+VL+ H ++N+D T+ +DNEA + I
Sbjct: 158 LERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDIC 217
Query: 728 ATGLSRYRTNVRDLN 772
L R +LN
Sbjct: 218 RRNLDIERPTYENLN 232
Score = 42.3 bits (95), Expect = 7e-05
Identities = 20/53 (37%), Positives = 30/53 (56%)
Frame = +1
Query: 82 MREIVHLQAGQCGNQIGAKFWEIISEEHGIDPTGVYRGTSDLQLERISVYYNE 240
MRE++ + GQ G QIG WE+ EHGI P G T + ++ + + Y N+
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGF--PTENSEVHKNNSYLND 51
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 79.0 bits (186), Expect = 7e-16
Identities = 33/82 (40%), Positives = 54/82 (65%), Gaps = 2/82 (2%)
Frame = +3
Query: 270 KYVPRAILLDLEPGTMDAVRSGAYGQLFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDA 443
+Y+PRAIL+DLEP ++ + S YG L+ P+N + ++ GAGNNWA G Y+ + +
Sbjct: 59 RYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFED 117
Query: 444 VLDVVRKECENCDCLQGFQLTH 509
++D++ +E + D L+GF L H
Sbjct: 118 IMDMIDREADGSDSLEGFSLLH 139
Score = 57.6 bits (133), Expect = 2e-09
Identities = 24/53 (45%), Positives = 36/53 (67%)
Frame = +1
Query: 85 REIVHLQAGQCGNQIGAKFWEIISEEHGIDPTGVYRGTSDLQLERISVYYNEA 243
REI+ LQAGQCGNQIG++FW+ + EHGI P G + ++R V++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQS 55
Score = 55.2 bits (127), Expect = 1e-08
Identities = 24/56 (42%), Positives = 37/56 (66%), Gaps = 1/56 (1%)
Frame = +2
Query: 551 ISKIREEYPDRIMNTYSVVP-SPKVSDTVVEPYNAVLSIHQLVENTDETYCIDNEA 715
+ ++ + YP +I+ TYSV P S VSD VV+PYN++L++ +L N D +DN A
Sbjct: 154 LERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAA 209
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 28.7 bits (61), Expect = 0.98
Identities = 14/30 (46%), Positives = 18/30 (60%)
Frame = +3
Query: 402 AKGHYTEGAELVDAVLDVVRKECENCDCLQ 491
A+GH G ELV A D +RK+ EN L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPBC16D10.03 |pgp2||metallopeptidase Pgp2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 346
Score = 28.3 bits (60), Expect = 1.3
Identities = 14/30 (46%), Positives = 18/30 (60%)
Frame = +2
Query: 674 VENTDETYCIDNEAFTISATGLSRYRTNVR 763
V +TDE +CIDN I+ GL Y+T R
Sbjct: 295 VFSTDERFCIDN-GIMIAQAGLLAYKTGDR 323
>SPCC16C4.07 |scw1||RNA-binding protein Scw1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 561
Score = 26.6 bits (56), Expect = 3.9
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = +1
Query: 640 TIQRSSLHPSTSREYRRNLLHRQ*GLYDICYRT 738
TI +L PSTS E + L Q G +C+RT
Sbjct: 427 TIYVGNLPPSTSEEELKVLFSTQVGYKRLCFRT 459
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 26.6 bits (56), Expect = 3.9
Identities = 10/34 (29%), Positives = 21/34 (61%)
Frame = +1
Query: 121 NQIGAKFWEIISEEHGIDPTGVYRGTSDLQLERI 222
N++G E+++E+ +DPT + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 25.4 bits (53), Expect = 9.1
Identities = 14/35 (40%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +3
Query: 333 GAYGQLFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 434
G YG +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,577,991
Number of Sequences: 5004
Number of extensions: 46158
Number of successful extensions: 145
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 134
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 143
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 373338084
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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