Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--1244X
         (525 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase |Schizosaccharom...    60   2e-10
SPBC902.05c |idh2|glu2|isocitrate dehydrogenase |Schizosaccharom...    60   2e-10
SPCP1E11.03 |mug170||arrestin|Schizosaccharomyces pombe|chr 3|||...    29   0.42 
SPBC1A4.02c |leu1|SPBC1E8.07c|3-isopropylmalate dehydrogenase Le...    29   0.42 
SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr 2|||M...    28   0.74 
SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces ...    27   1.3  
SPBC3E7.15c |mug83|SPBC4F6.02c|sphingosine N-acyltransferase Lac...    27   1.7  
SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor Sp...    27   1.7  
SPBC365.09c |||human KIN homolog|Schizosaccharomyces pombe|chr 2...    27   1.7  
SPAC19G12.02c |pms1||MutL family mismatch-repair protein Pms1|Sc...    27   2.3  
SPAC1071.02 |||TFIIH regulator |Schizosaccharomyces pombe|chr 1|...    26   4.0  
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc...    25   6.9  
SPCC550.15c |||ribosome biogenesis protein |Schizosaccharomyces ...    25   6.9  
SPCC1223.13 |cbf12||CBF1/Su|Schizosaccharomyces pombe|chr 3|||Ma...    25   6.9  
SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces ...    25   9.1  
SPAC343.10 |met11|mthfr2|methylenetetrahydrofolate reductase Met...    25   9.1  
SPBC11C11.05 |||KRE9 family cell wall biosynthesis protein |Schi...    25   9.1  

>SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 356

 Score = 60.5 bits (140), Expect = 2e-10
 Identities = 30/75 (40%), Positives = 45/75 (60%), Gaps = 3/75 (4%)
 Frame = +3

Query: 249 GRRIKCTLIPGDGVGPELVYAVQEVFKAASIPVDFESFFFSEV---NPTLSAPLEDVVNS 419
           G +   TLIPGDG+G E   AV E+FK A++P++FE    + +   N +    L + + S
Sbjct: 18  GGKYTVTLIPGDGIGRETSNAVTEIFKTANVPIEFEEIDVTGMEKNNKSSGDALHEAIQS 77

Query: 420 IAVNKICIKGILATP 464
           +  NK+ +KGIL TP
Sbjct: 78  LKRNKVGLKGILFTP 92


>SPBC902.05c |idh2|glu2|isocitrate dehydrogenase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 378

 Score = 60.1 bits (139), Expect = 2e-10
 Identities = 28/68 (41%), Positives = 44/68 (64%), Gaps = 2/68 (2%)
 Frame = +3

Query: 267 TLIPGDGVGPELVYAVQEVFKAASIPVDFESFFFSEV--NPTLSAPLEDVVNSIAVNKIC 440
           T+I GDG+GPE+  +V+ +FKAA +P+++E      +  N T + P +D   S+  NK+ 
Sbjct: 49  TMIAGDGIGPEIAQSVERIFKAAKVPIEWERVKVYPILKNGTTTIP-DDAKESVRKNKVA 107

Query: 441 IKGILATP 464
           +KG LATP
Sbjct: 108 LKGPLATP 115


>SPCP1E11.03 |mug170||arrestin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 426

 Score = 29.1 bits (62), Expect = 0.42
 Identities = 16/45 (35%), Positives = 25/45 (55%), Gaps = 1/45 (2%)
 Frame = +3

Query: 138 WKGCTHKFGNNGEKCMLCTIWSPA-IFNKVQIATEGNKGRRIKCT 269
           +KG +  F +  EK   C + + A I +K   A+ GNKG  +KC+
Sbjct: 140 FKGHSEPFYSISEKYSFCNLKTSALILSKAASASIGNKGLFLKCS 184


>SPBC1A4.02c |leu1|SPBC1E8.07c|3-isopropylmalate dehydrogenase
           Leu1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 371

 Score = 29.1 bits (62), Expect = 0.42
 Identities = 11/23 (47%), Positives = 16/23 (69%)
 Frame = +3

Query: 261 KCTLIPGDGVGPELVYAVQEVFK 329
           K  ++PGD +GPE+V +  EV K
Sbjct: 5   KIVVLPGDHIGPEIVASALEVLK 27


>SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 685

 Score = 28.3 bits (60), Expect = 0.74
 Identities = 11/19 (57%), Positives = 13/19 (68%)
 Frame = -3

Query: 202 LQMVHNIHFSPLLPNLCVH 146
           LQ  H++H SPL P  CVH
Sbjct: 261 LQGTHSVHGSPLKPEDCVH 279


>SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 362

 Score = 27.5 bits (58), Expect = 1.3
 Identities = 12/26 (46%), Positives = 17/26 (65%)
 Frame = +3

Query: 252 RRIKCTLIPGDGVGPELVYAVQEVFK 329
           RRI   LIP DG+G E+V A + + +
Sbjct: 5   RRIVLGLIPADGIGKEVVPAARRLME 30


>SPBC3E7.15c |mug83|SPBC4F6.02c|sphingosine N-acyltransferase
           Lac1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 384

 Score = 27.1 bits (57), Expect = 1.7
 Identities = 12/32 (37%), Positives = 16/32 (50%)
 Frame = -2

Query: 437 NLVNSDGVYDVFEWCTKCWIHFRKEKGFKVHW 342
           N VN+  VY +F      WI+ R    FK+ W
Sbjct: 269 NYVNTVIVYPIFVIFVFVWIYMRHYLNFKIMW 300


>SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor
           Spt6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1365

 Score = 27.1 bits (57), Expect = 1.7
 Identities = 11/37 (29%), Positives = 24/37 (64%), Gaps = 1/37 (2%)
 Frame = -2

Query: 440 TNLVNSDGVYDVFEWCTKCW-IHFRKEKGFKVHWNAG 333
           T L++ + ++++F  CTK W +H +K+   K++ + G
Sbjct: 355 TPLLSQNDLWNIFFLCTKFWSLHSKKQDILKLYSDLG 391


>SPBC365.09c |||human KIN homolog|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 304

 Score = 27.1 bits (57), Expect = 1.7
 Identities = 11/32 (34%), Positives = 17/32 (53%)
 Frame = -2

Query: 440 TNLVNSDGVYDVFEWCTKCWIHFRKEKGFKVH 345
           +N + S G+  +  +C+ C    R E GFK H
Sbjct: 13  SNALKSKGLQRLRWYCSACQKQMRDENGFKCH 44


>SPAC19G12.02c |pms1||MutL family mismatch-repair protein
           Pms1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 794

 Score = 26.6 bits (56), Expect = 2.3
 Identities = 21/70 (30%), Positives = 34/70 (48%)
 Frame = +3

Query: 294 PELVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIKGILATPDFS 473
           P    A+Q++    S P+D  + F  ++N +LS   +D+V S A+ K   K I    D S
Sbjct: 533 PSKTAALQKLKFFQSRPLDGLNKFSKKINISLSGVQKDIVRSDALLKFSNK-IGVVHDIS 591

Query: 474 HTGELQTLNM 503
              +   LN+
Sbjct: 592 DENQEDHLNL 601


>SPAC1071.02 |||TFIIH regulator |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1018

 Score = 25.8 bits (54), Expect = 4.0
 Identities = 13/44 (29%), Positives = 24/44 (54%)
 Frame = +3

Query: 381  PTLSAPLEDVVNSIAVNKICIKGILATPDFSHTGELQTLNMKLV 512
            P+L A  +D+ N   V  + +K + + P+F+ T  LQ    K++
Sbjct: 948  PSLLAIPKDMNNPTVVRLLALKCLGSLPEFTPTTNLQLFRDKVI 991


>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 613

 Score = 25.0 bits (52), Expect = 6.9
 Identities = 10/15 (66%), Positives = 12/15 (80%)
 Frame = -2

Query: 284 VPRNQGAFDTPSFVA 240
           +P +QGA  TPSFVA
Sbjct: 31  IPNDQGARTTPSFVA 45


>SPCC550.15c |||ribosome biogenesis protein |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 463

 Score = 25.0 bits (52), Expect = 6.9
 Identities = 7/20 (35%), Positives = 11/20 (55%)
 Frame = -2

Query: 395 CTKCWIHFRKEKGFKVHWNA 336
           CT C + F   +  K+HW +
Sbjct: 7   CTTCTVAFNNAESQKIHWKS 26


>SPCC1223.13 |cbf12||CBF1/Su|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 963

 Score = 25.0 bits (52), Expect = 6.9
 Identities = 9/21 (42%), Positives = 13/21 (61%)
 Frame = +2

Query: 167 QRRKMYVMHHLEPCYFQQSPN 229
           ++ K Y   H E  Y+QQSP+
Sbjct: 341 EKEKSYSQDHAELSYYQQSPS 361


>SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1098

 Score = 24.6 bits (51), Expect = 9.1
 Identities = 12/46 (26%), Positives = 20/46 (43%)
 Frame = +3

Query: 369 SEVNPTLSAPLEDVVNSIAVNKICIKGILATPDFSHTGELQTLNMK 506
           S++N    A LED       N +C++  +A    S    LQ +  +
Sbjct: 541 SQLNVFTKASLEDKQKKEKYNLLCVRSCIAIDSISWISSLQGVKFR 586


>SPAC343.10 |met11|mthfr2|methylenetetrahydrofolate reductase
           Met11|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 641

 Score = 24.6 bits (51), Expect = 9.1
 Identities = 18/65 (27%), Positives = 28/65 (43%), Gaps = 9/65 (13%)
 Frame = +3

Query: 282 DGVGPELVYAVQEVFKAASIPVD-------FESFFFSEVN--PTLSAPLEDVVNSIAVNK 434
           DG GP L +   E  K    PVD       F+    S+++  P +  P+E    +IA   
Sbjct: 415 DGYGPTLHFPPSEALKLWGYPVDESDITSLFQKHIMSDISAIPWIDEPVEVETKTIAKYL 474

Query: 435 ICIKG 449
           + + G
Sbjct: 475 LKLNG 479


>SPBC11C11.05 |||KRE9 family cell wall biosynthesis protein
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 284

 Score = 24.6 bits (51), Expect = 9.1
 Identities = 11/37 (29%), Positives = 19/37 (51%)
 Frame = +3

Query: 312 VQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSI 422
           ++ V  A +  +DF +  FS        PLED+ N++
Sbjct: 23  IRFVTPATTDSMDFTAISFSWEESNTGIPLEDITNTV 59


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,268,965
Number of Sequences: 5004
Number of extensions: 50167
Number of successful extensions: 150
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 214353836
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)