Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1244X
(525 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g35650.1 68417.m05062 isocitrate dehydrogenase, putative / NA... 64 6e-11
At3g09810.1 68416.m01169 isocitrate dehydrogenase, putative / NA... 64 6e-11
At2g17130.2 68415.m01978 isocitrate dehydrogenase subunit 2 / NA... 62 2e-10
At2g17130.1 68415.m01977 isocitrate dehydrogenase subunit 2 / NA... 62 2e-10
At4g35260.1 68417.m05011 isocitrate dehydrogenase subunit 1 / NA... 60 1e-09
At5g03290.1 68418.m00279 isocitrate dehydrogenase, putative / NA... 59 2e-09
At1g31180.1 68414.m03817 3-isopropylmalate dehydrogenase, chloro... 36 0.022
At1g80560.1 68414.m09445 3-isopropylmalate dehydrogenase, chloro... 34 0.051
At5g14200.1 68418.m01659 3-isopropylmalate dehydrogenase, chloro... 33 0.12
At1g14820.3 68414.m01773 SEC14 cytosolic factor family protein /... 30 0.83
At1g14820.2 68414.m01772 SEC14 cytosolic factor family protein /... 30 0.83
At1g14820.1 68414.m01771 SEC14 cytosolic factor family protein /... 30 0.83
At5g60620.1 68418.m07608 phospholipid/glycerol acyltransferase f... 28 4.4
At3g56150.1 68416.m06241 eukaryotic translation initiation facto... 27 7.7
>At4g35650.1 68417.m05062 isocitrate dehydrogenase, putative / NAD+
isocitrate dehydrogenase, putative strong similarity to
NAD+ dependent isocitrate dehydrogenase subunit 1
[Arabidopsis thaliana] GI:1766046
Length = 368
Score = 64.1 bits (149), Expect = 6e-11
Identities = 36/81 (44%), Positives = 51/81 (62%)
Frame = +3
Query: 267 TLIPGDGVGPELVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIK 446
TLIPGDG+GP + AV++V +A PV FE + EV + E+V+ S+ NK+C+K
Sbjct: 42 TLIPGDGIGPLVTGAVEQVMEAMHAPVHFERY---EVLGNMRKVPEEVIESVKRNKVCLK 98
Query: 447 GILATPDFSHTGELQTLNMKL 509
G LATP G + +LNM+L
Sbjct: 99 GGLATP---VGGGVSSLNMQL 116
>At3g09810.1 68416.m01169 isocitrate dehydrogenase, putative / NAD+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase (NAD+) GB:CAA65502 GI:3021506
[Nicotiana tabacum]
Length = 374
Score = 64.1 bits (149), Expect = 6e-11
Identities = 32/71 (45%), Positives = 49/71 (69%), Gaps = 2/71 (2%)
Frame = +3
Query: 258 IKCTLIPGDGVGPELVYAVQEVFKAASIPVDF-ESFFFSEVNPTLSAPLE-DVVNSIAVN 431
IK TL PGDG+GPE+ +V++VF AA + +D+ E F +EV+P ++ L D + S+ N
Sbjct: 44 IKATLFPGDGIGPEIAESVKQVFTAADVVIDWDEQFVGTEVDPRTNSFLTWDNLQSVLKN 103
Query: 432 KICIKGILATP 464
K+ +KG +ATP
Sbjct: 104 KVGLKGPMATP 114
>At2g17130.2 68415.m01978 isocitrate dehydrogenase subunit 2 / NAD+
isocitrate dehydrogenase subunit 2 nearly identical to
NAD+ dependent isocitrate dehydrogenase subunit 2
[Arabidopsis thaliana] GI:1766048
Length = 363
Score = 62.5 bits (145), Expect = 2e-10
Identities = 38/87 (43%), Positives = 52/87 (59%)
Frame = +3
Query: 249 GRRIKCTLIPGDGVGPELVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAV 428
G+ TLIPGDGVGP + AVQ+V +A PV FE F EV+ + + E ++ SI
Sbjct: 35 GKPRPVTLIPGDGVGPLVTNAVQQVMEAMHAPVYFEPF---EVHGDMKSLPEGLLESIKK 91
Query: 429 NKICIKGILATPDFSHTGELQTLNMKL 509
NK+C+KG L TP G + +LN+ L
Sbjct: 92 NKVCLKGGLKTP---VGGGVSSLNVNL 115
>At2g17130.1 68415.m01977 isocitrate dehydrogenase subunit 2 / NAD+
isocitrate dehydrogenase subunit 2 nearly identical to
NAD+ dependent isocitrate dehydrogenase subunit 2
[Arabidopsis thaliana] GI:1766048
Length = 367
Score = 62.5 bits (145), Expect = 2e-10
Identities = 38/87 (43%), Positives = 52/87 (59%)
Frame = +3
Query: 249 GRRIKCTLIPGDGVGPELVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAV 428
G+ TLIPGDGVGP + AVQ+V +A PV FE F EV+ + + E ++ SI
Sbjct: 35 GKPRPVTLIPGDGVGPLVTNAVQQVMEAMHAPVYFEPF---EVHGDMKSLPEGLLESIKK 91
Query: 429 NKICIKGILATPDFSHTGELQTLNMKL 509
NK+C+KG L TP G + +LN+ L
Sbjct: 92 NKVCLKGGLKTP---VGGGVSSLNVNL 115
>At4g35260.1 68417.m05011 isocitrate dehydrogenase subunit 1 / NAD+
isocitrate dehydrogenase subunit 1 nearly identical to
NAD+ dependent isocitrate dehydrogenase subunit 1
[Arabidopsis thaliana] GI:1766046
Length = 367
Score = 59.7 bits (138), Expect = 1e-09
Identities = 33/81 (40%), Positives = 51/81 (62%)
Frame = +3
Query: 267 TLIPGDGVGPELVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIK 446
TLIPGDG+GP + AV++V +A P+ FE + +V+ +S +V+ SI NK+C+K
Sbjct: 41 TLIPGDGIGPLVTNAVEQVMEAMHAPIFFEKY---DVHGEMSRVPPEVMESIRKNKVCLK 97
Query: 447 GILATPDFSHTGELQTLNMKL 509
G L TP G + +LN++L
Sbjct: 98 GGLKTP---VGGGVSSLNVQL 115
>At5g03290.1 68418.m00279 isocitrate dehydrogenase, putative / NAD+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase (NAD+) [Nicotiana tabacum]
GI:3021506
Length = 374
Score = 58.8 bits (136), Expect = 2e-09
Identities = 28/71 (39%), Positives = 47/71 (66%), Gaps = 2/71 (2%)
Frame = +3
Query: 258 IKCTLIPGDGVGPELVYAVQEVFKAASIPVDFESFFF-SEVNP-TLSAPLEDVVNSIAVN 431
I TL PGDG+GPE+ +V++VF A +P+++E + +E++P T S + + S+ N
Sbjct: 44 ITATLFPGDGIGPEIAESVKKVFTTAGVPIEWEEHYVGTEIDPRTQSFLTWESLESVRRN 103
Query: 432 KICIKGILATP 464
K+ +KG +ATP
Sbjct: 104 KVGLKGPMATP 114
>At1g31180.1 68414.m03817 3-isopropylmalate dehydrogenase,
chloroplast, putative strong similarity to SP|P29102
3-isopropylmalate dehydrogenase, chloroplast precursor
{Brassica napus}; EST gb|F14478 comes from this gene
Length = 404
Score = 35.5 bits (78), Expect = 0.022
Identities = 17/53 (32%), Positives = 28/53 (52%)
Frame = +3
Query: 219 KVQIATEGNKGRRIKCTLIPGDGVGPELVYAVQEVFKAASIPVDFESFFFSEV 377
+++ A +R TL+PGDG+GPE++ + V + A E F F E+
Sbjct: 31 RIRCAAASPVKKRYNITLLPGDGIGPEVISVAKNVLQKAGFLQGLE-FDFQEM 82
>At1g80560.1 68414.m09445 3-isopropylmalate dehydrogenase,
chloroplast, putative strong similarity to
3-ISOPROPYLMALATE DEHYDROGENASE PRECURSOR GB:P29102
SP|P29102 from [Brassica napus]
Length = 405
Score = 34.3 bits (75), Expect = 0.051
Identities = 16/36 (44%), Positives = 23/36 (63%)
Frame = +3
Query: 228 IATEGNKGRRIKCTLIPGDGVGPELVYAVQEVFKAA 335
+A+ G K R TL+PGDG+GPE+V + V + A
Sbjct: 35 VASPGKK--RYTITLLPGDGIGPEVVSIAKNVLQQA 68
>At5g14200.1 68418.m01659 3-isopropylmalate dehydrogenase,
chloroplast, putative strong similarity to SP|P29102
3-isopropylmalate dehydrogenase, chloroplast precursor
{Brassica napus}
Length = 409
Score = 33.1 bits (72), Expect = 0.12
Identities = 19/62 (30%), Positives = 31/62 (50%)
Frame = +3
Query: 219 KVQIATEGNKGRRIKCTLIPGDGVGPELVYAVQEVFKAASIPVDFESFFFSEVNPTLSAP 398
+++ A +R L+PGDG+GPE++ + V + A E F F E+ P A
Sbjct: 34 RIRCAAASPGKKRYNIALLPGDGIGPEVISVAKNVLQKAGSLEGLE-FDFKEM-PVGGAA 91
Query: 399 LE 404
L+
Sbjct: 92 LD 93
>At1g14820.3 68414.m01773 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
PF00650 : CRAL/TRIO domain; similar to
phosphatidylinositol/phosphatidylcholine transfer
protein SP:P24280 [Saccharomyces cerevisiae (Baker's
yeast)]
Length = 252
Score = 30.3 bits (65), Expect = 0.83
Identities = 16/50 (32%), Positives = 27/50 (54%)
Frame = +3
Query: 300 LVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIKG 449
LV + KAA + VD++ + S V PT P +V + + K+C++G
Sbjct: 35 LVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDELEFRKVCLQG 84
>At1g14820.2 68414.m01772 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
PF00650 : CRAL/TRIO domain; similar to
phosphatidylinositol/phosphatidylcholine transfer
protein SP:P24280 [Saccharomyces cerevisiae (Baker's
yeast)]
Length = 239
Score = 30.3 bits (65), Expect = 0.83
Identities = 16/50 (32%), Positives = 27/50 (54%)
Frame = +3
Query: 300 LVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIKG 449
LV + KAA + VD++ + S V PT P +V + + K+C++G
Sbjct: 22 LVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDELEFRKVCLQG 71
>At1g14820.1 68414.m01771 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
PF00650 : CRAL/TRIO domain; similar to
phosphatidylinositol/phosphatidylcholine transfer
protein SP:P24280 [Saccharomyces cerevisiae (Baker's
yeast)]
Length = 239
Score = 30.3 bits (65), Expect = 0.83
Identities = 16/50 (32%), Positives = 27/50 (54%)
Frame = +3
Query: 300 LVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIKG 449
LV + KAA + VD++ + S V PT P +V + + K+C++G
Sbjct: 22 LVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDELEFRKVCLQG 71
>At5g60620.1 68418.m07608 phospholipid/glycerol acyltransferase
family protein contains Pfam PF01553: Acyltransferase
Length = 376
Score = 27.9 bits (59), Expect = 4.4
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = -2
Query: 122 CITARKIFLLRKDMFEFDKNVC*IYIFSNKLFME 21
C+ + +K FE D VC I I NK+F++
Sbjct: 248 CVNNNYTVMFKKGAFELDCTVCPIAIKYNKIFVD 281
>At3g56150.1 68416.m06241 eukaryotic translation initiation factor 3
subunit 8 / eIF3 p110 / eIF3c / p105 (TIF3C1) nearly
identical to SP|O49160 Eukaryotic translation initiation
factor 3 subunit 8 (eIF3 p110) (eIF3c) (p105)
{Arabidopsis thaliana}
Length = 900
Score = 27.1 bits (57), Expect = 7.7
Identities = 20/76 (26%), Positives = 36/76 (47%), Gaps = 2/76 (2%)
Frame = +2
Query: 53 FNKRFYQIQTCPFLAGIFFELLCRDLNMLERVYTQVR*QRRKMYVMHHLEPCYFQQSPNS 232
FN+ Q+ C F AG+ E + L +Y+ R R++ + Y +++P
Sbjct: 540 FNRTMAQLGLCAFRAGMITE----SHSCLSELYSGQR--VRELLAQGVSQSRYHEKTPEQ 593
Query: 233 NRGQQRK--AYQMHLD 274
R ++R+ Y MHL+
Sbjct: 594 ERMERRRQMPYHMHLN 609
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,674,336
Number of Sequences: 28952
Number of extensions: 253204
Number of successful extensions: 637
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 618
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 631
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 967280384
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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