BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--1226 (635 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1919.03c |||AMP-activated protein kinase beta subunit |Schiz... 42 1e-04 SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit ... 27 1.7 SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic... 27 3.0 SPCC1672.06c |asp1|vip1|inositol hexakisphosphate kinase/inosito... 27 3.0 SPCC663.10 |||methyltransferase, DUF1613 family |Schizosaccharom... 27 3.0 SPCC1672.11c |||P-type ATPase |Schizosaccharomyces pombe|chr 3||... 26 4.0 SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-pho... 26 4.0 SPAPB2B4.02 |grx5||monothiol glutaredoxin Grx5|Schizosaccharomyc... 26 4.0 SPCC61.05 |||S. pombe specific multicopy membrane protein family... 25 6.9 >SPCC1919.03c |||AMP-activated protein kinase beta subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 298 Score = 41.5 bits (93), Expect = 1e-04 Identities = 25/87 (28%), Positives = 45/87 (51%), Gaps = 3/87 (3%) Frame = +3 Query: 258 DDNNIEDGAPYYTKAVPESDIEYTEQRERSNTLTDGSKIVDDIK--VLPTVFKWEGGGKQ 431 DDN E G ++ +T++R + T G K +PT+ +W GGG+ Sbjct: 56 DDNQQEGGNNRTSQNGTSGSSGHTKRRSQ----TSGKKTHQPYSGPCVPTIIRWRGGGEV 111 Query: 432 VFISGTFTDW-KTIPMVKSHGDFVTIM 509 V+++G+F+ W K I ++KS D+ ++ Sbjct: 112 VYVTGSFSRWKKKIQLLKSE-DYTVLL 137 >SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit Bgs2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1894 Score = 27.5 bits (58), Expect = 1.7 Identities = 10/22 (45%), Positives = 15/22 (68%) Frame = -3 Query: 156 SIFMPFFGLVTSGIPHLIFKWL 91 ++ M FG +T IPHLI+ W+ Sbjct: 1492 TLLMLLFGTMTVWIPHLIYFWI 1513 >SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic subunit Bgs1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1729 Score = 26.6 bits (56), Expect = 3.0 Identities = 8/21 (38%), Positives = 15/21 (71%) Frame = -3 Query: 153 IFMPFFGLVTSGIPHLIFKWL 91 ++M FG +T+ +PH I+ W+ Sbjct: 1335 MYMLLFGSITAWLPHYIYFWI 1355 >SPCC1672.06c |asp1|vip1|inositol hexakisphosphate kinase/inositol pyrophosphate synthase |Schizosaccharomyces pombe|chr 3|||Manual Length = 920 Score = 26.6 bits (56), Expect = 3.0 Identities = 14/45 (31%), Positives = 25/45 (55%) Frame = +1 Query: 115 NAGSNQPKEWHKDANKSPDHDQGPSSPAKEGEAFTFDKKLDDDRS 249 + G K + K ANKS D+D +P EG +F +++ ++ D + Sbjct: 213 SVGGGGRKLFRKVANKSSDYDPDLCAPRTEG-SFIYEEFMNVDNA 256 >SPCC663.10 |||methyltransferase, DUF1613 family |Schizosaccharomyces pombe|chr 3|||Manual Length = 502 Score = 26.6 bits (56), Expect = 3.0 Identities = 9/22 (40%), Positives = 13/22 (59%) Frame = +2 Query: 80 LHTYNHLKIKWGMPEVTNPKNG 145 +H KI W +P+ NPK+G Sbjct: 397 VHDLTGAKISWALPKPRNPKHG 418 >SPCC1672.11c |||P-type ATPase |Schizosaccharomyces pombe|chr 3|||Manual Length = 1315 Score = 26.2 bits (55), Expect = 4.0 Identities = 12/22 (54%), Positives = 16/22 (72%), Gaps = 2/22 (9%) Frame = -3 Query: 132 LVTSGIPHLIFKWL--YVCRFV 73 L+T G+ +LIF+WL Y RFV Sbjct: 176 LLTLGLAYLIFRWLPKYFIRFV 197 >SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-phosphate 5-kinase Fab1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1932 Score = 26.2 bits (55), Expect = 4.0 Identities = 12/40 (30%), Positives = 18/40 (45%) Frame = -3 Query: 195 WR*RSLIMIRRFVSIFMPFFGLVTSGIPHLIFKWLYVCRF 76 W RS + +S+F+ F G+ I W Y C+F Sbjct: 984 WHYRSYVHGNSRISVFLESFSCPVPGLEEKIIMWSY-CKF 1022 >SPAPB2B4.02 |grx5||monothiol glutaredoxin Grx5|Schizosaccharomyces pombe|chr 1|||Manual Length = 146 Score = 26.2 bits (55), Expect = 4.0 Identities = 9/17 (52%), Positives = 13/17 (76%) Frame = +3 Query: 450 FTDWKTIPMVKSHGDFV 500 F+DW TIP + +G+FV Sbjct: 89 FSDWPTIPQLYINGEFV 105 >SPCC61.05 |||S. pombe specific multicopy membrane protein family 1|Schizosaccharomyces pombe|chr 3|||Manual Length = 469 Score = 25.4 bits (53), Expect = 6.9 Identities = 10/31 (32%), Positives = 20/31 (64%) Frame = -2 Query: 424 PPPSHLKTVGNTLISSTILLPSVRVFDLSRC 332 PP S+ +T+ +TL++ ++ L + + L RC Sbjct: 191 PPESYFRTIKSTLLAISMFLGFITLTWLLRC 221 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,727,642 Number of Sequences: 5004 Number of extensions: 60811 Number of successful extensions: 147 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 145 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 147 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 283719918 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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