Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1218X
(527 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g02720.1 68415.m00214 pectate lyase family protein similar to... 31 0.48
At4g36520.1 68417.m05185 trichohyalin-related low similarity to ... 31 0.63
At5g11110.1 68418.m01297 sucrose-phosphate synthase, putative si... 30 0.84
At1g51900.1 68414.m05850 hypothetical protein 29 1.5
At1g09140.1 68414.m01018 SF2/ASF-like splicing modulator (SRP30)... 29 1.5
At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam d... 29 1.9
At1g56040.1 68414.m06434 U-box domain-containing protein contain... 29 1.9
At3g28770.1 68416.m03591 expressed protein 29 2.6
At1g23230.1 68414.m02906 expressed protein 29 2.6
At4g31820.1 68417.m04522 phototropic-responsive NPH3 family prot... 28 3.4
At4g05280.1 68417.m00799 Ulp1 protease family protein contains P... 28 3.4
At3g57780.1 68416.m06436 expressed protein 28 3.4
At3g43390.1 68416.m04592 hypothetical protein similar to At3g243... 28 3.4
At3g02320.1 68416.m00214 N2,N2-dimethylguanosine tRNA methyltran... 28 3.4
At3g19840.1 68416.m02513 FF domain-containing protein / WW domai... 27 5.9
At2g29210.1 68415.m03550 splicing factor PWI domain-containing p... 27 5.9
At3g54290.1 68416.m06000 expressed protein contains Pfam PF03794... 27 7.8
>At2g02720.1 68415.m00214 pectate lyase family protein similar to
pectate lyase P59 SP:P15722 from [Lycopersicon
esculentum]
Length = 455
Score = 31.1 bits (67), Expect = 0.48
Identities = 17/58 (29%), Positives = 24/58 (41%)
Frame = +1
Query: 271 KHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFDPTKLKHTETCEKNPLPTKDVIEQE 444
K +E P P+ + + EK+ FLNG K KNP+P K I +
Sbjct: 375 KQVTKREYTPYPEWKSWNWQSEKDYFLNGAYFVQSGKANAWSATPKNPIPRKFAIRPQ 432
>At4g36520.1 68417.m05185 trichohyalin-related low similarity to
SP|Q07283 Trichohyalin {Homo sapiens}
Length = 1400
Score = 30.7 bits (66), Expect = 0.63
Identities = 17/68 (25%), Positives = 33/68 (48%), Gaps = 1/68 (1%)
Frame = +1
Query: 247 REVDSSQLKHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFD-PTKLKHTETCEKNPLPT 423
+E Q+K + +E+N K+ +E + + K +E + +LK T E+N
Sbjct: 772 QEEKERQIKERQEREENERRAKEVLEQAENERKLKEALEQKENERRLKETREKEENKKKL 831
Query: 424 KDVIEQEK 447
++ IE E+
Sbjct: 832 REAIELEE 839
>At5g11110.1 68418.m01297 sucrose-phosphate synthase, putative
similar to sucrose-phosphate synthase isoform 1, Citrus
unshiu, PIR:S72648
Length = 894
Score = 30.3 bits (65), Expect = 0.84
Identities = 22/59 (37%), Positives = 32/59 (54%), Gaps = 3/59 (5%)
Frame = +2
Query: 68 SVSDTPS--LKDLXKVATDLKSQLEGFNTSCLGDVDTNEKIVLRSAEDVA-TEKTQKSL 235
S SD+PS L+D+ ++ +LK L+G + VDTN R+AE A EK +L
Sbjct: 532 SDSDSPSDSLRDINDISLNLKLSLDGEKSGSNNGVDTNLDAEDRAAERKAEVEKAVSTL 590
>At1g51900.1 68414.m05850 hypothetical protein
Length = 774
Score = 29.5 bits (63), Expect = 1.5
Identities = 22/76 (28%), Positives = 36/76 (47%)
Frame = +1
Query: 220 DPEVFIRRYREVDSSQLKHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFDPTKLKHTET 399
DP+++IR Y E + K + T + + + D+IE K + L+ I LK +
Sbjct: 362 DPDIYIRSYEESPNEVYKFSLTDLEEEIMENDSIEG--VKCRMLDEIMKKSGHHLKISRP 419
Query: 400 CEKNPLPTKDVIEQEK 447
E P K V E+E+
Sbjct: 420 -EYKPEIEKQVYEEEE 434
>At1g09140.1 68414.m01018 SF2/ASF-like splicing modulator (SRP30)
nearly identical to SF2/ASF-like splicing modulator
Srp30 [Arabidopsis thaliana] GI:4775270
Length = 268
Score = 29.5 bits (63), Expect = 1.5
Identities = 15/35 (42%), Positives = 18/35 (51%)
Frame = +3
Query: 201 KTSPLRRPRSLYSTVSRS*FEPAEAHRDSGEEPAS 305
++ PL R RSLYS+VSRS R P S
Sbjct: 229 RSRPLSRSRSLYSSVSRSQSRSKSRSRSRSNSPVS 263
>At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam
domain PF04931: DNA polymerase V
Length = 1306
Score = 29.1 bits (62), Expect = 1.9
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = +1
Query: 277 TETQEKNPLPDKDAIEAEKEKNKFLNGIENFD 372
TE E + LPD ++ +K K + +N + N+D
Sbjct: 12 TELVENDNLPDSSIVKKKKSKTEKMNTVANYD 43
>At1g56040.1 68414.m06434 U-box domain-containing protein contains
Pfam profile PF04564: U-box domain
Length = 437
Score = 29.1 bits (62), Expect = 1.9
Identities = 33/127 (25%), Positives = 55/127 (43%), Gaps = 5/127 (3%)
Frame = +1
Query: 85 LPERPXQGRNRLEESARRLQHKLSR*RRHQ*KDCASVC*RRRH*EDPEVFIRRY-REVDS 261
L E + + +L+ A+ L+HKL RH+ + + R E ++ + E+D+
Sbjct: 175 LLESYKEEQGKLQLQAKALEHKLEAELRHRKETETLLAIERDRIEKVKIQLETVENEIDN 234
Query: 262 SQLKHTETQEKNP----LPDKDAIEAEKEKNKFLNGIENFDPTKLKHTETCEKNPLPTKD 429
++LK E + K L + I EKEK K L ++ T + E +D
Sbjct: 235 TRLKAEEFERKYEGEMILRRESEIALEKEK-KELEEVKLKLETYEREQENLSSEVRTWQD 293
Query: 430 VIEQEKS 450
EQE S
Sbjct: 294 KYEQESS 300
>At3g28770.1 68416.m03591 expressed protein
Length = 2081
Score = 28.7 bits (61), Expect = 2.6
Identities = 20/66 (30%), Positives = 30/66 (45%), Gaps = 1/66 (1%)
Frame = +1
Query: 235 IRRYREVDSSQLKHTETQEKNPLPD-KDAIEAEKEKNKFLNGIENFDPTKLKHTETCEKN 411
+R E K +E EK + KDA E + NK L+ EN D K + E +++
Sbjct: 768 VRNKEENVQGNKKESEKVEKGEKKESKDAKSVETKDNKKLSSTENRDEAKERSGEDNKED 827
Query: 412 PLPTKD 429
+KD
Sbjct: 828 KEESKD 833
>At1g23230.1 68414.m02906 expressed protein
Length = 1615
Score = 28.7 bits (61), Expect = 2.6
Identities = 14/35 (40%), Positives = 20/35 (57%), Gaps = 1/35 (2%)
Frame = -2
Query: 427 PLWGAGSSRTFPCASA*WDRSSRC-RSGICSFPSP 326
PL+G + + P S W+R+ RC R I + PSP
Sbjct: 393 PLYGEDLAMSIPKGSLDWERAVRCIRHAIRTTPSP 427
>At4g31820.1 68417.m04522 phototropic-responsive NPH3 family protein
contains NPH3 family domain, Pfam:PF03000
Length = 571
Score = 28.3 bits (60), Expect = 3.4
Identities = 25/92 (27%), Positives = 41/92 (44%), Gaps = 10/92 (10%)
Frame = +3
Query: 123 RVSSKASTQAVSVTSTPMKRLCFGLLKTSPLRRPRSLYSTVSRS*FEPAE-AHRDSGEEP 299
+++++AST A P+ R+ +L LR S ++V+ S P E + GEE
Sbjct: 438 KLTNEASTHAAQNERLPL-RVVVQVLYFEQLRANHSPVASVAASSHSPVEKTEENKGEEA 496
Query: 300 AS---------GQRCYRSGEGKEQIPERHREL 368
G + RSG G + +P R R +
Sbjct: 497 TKKVELSKKSRGSKSTRSGGGAQLMPSRSRRI 528
>At4g05280.1 68417.m00799 Ulp1 protease family protein contains Pfam
profile PF02902: Ulp1 protease family, C-terminal
catalytic domain; similar to At3g24380, At5g36840,
At5g35010, At3g42740, At4g05290, At2g14770, At3g43390,
At2g05560, At4g08880, At1g34730, At1g27790, At1g34740,
At1g27780, At5g36850, At3g42730, At1g52020, At3g24390,
At1g25886, At4g03300
Length = 1312
Score = 28.3 bits (60), Expect = 3.4
Identities = 17/73 (23%), Positives = 32/73 (43%)
Frame = +1
Query: 232 FIRRYREVDSSQLKHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFDPTKLKHTETCEKN 411
+ R +++ QL+ T+ + K P E +K I D K ++ E C
Sbjct: 348 YARVPKQLRPPQLEETDVKRKRNAPGPSPKEPAMKKQ-----ISEMDCDKEENAEDCFGE 402
Query: 412 PLPTKDVIEQEKS 450
P+P + ++E +S
Sbjct: 403 PVPERFIVEMRRS 415
>At3g57780.1 68416.m06436 expressed protein
Length = 670
Score = 28.3 bits (60), Expect = 3.4
Identities = 20/51 (39%), Positives = 27/51 (52%), Gaps = 1/51 (1%)
Frame = +1
Query: 256 DSSQLKHTETQEK-NPLPDKDAIEAEKEKNKFLNGIENFDPTKLKHTETCE 405
DSS+ TE +E N L D E EKE++K L+ I + + ETCE
Sbjct: 72 DSSE--KTEKEETINGLACDDEDEEEKEESKELDAIAHEKTDSVSSPETCE 120
>At3g43390.1 68416.m04592 hypothetical protein similar to At3g24380,
At5g36840, At5g35010, At3g42740, At4g05290, At2g14770,
At2g05560, At4g08880, At1g34730, At1g27790, At1g34740,
At1g27780, At5g36850, At3g42730, At1g52020, At3g24390,
At4g05280, At1g25886, At4g03300
Length = 1113
Score = 28.3 bits (60), Expect = 3.4
Identities = 17/73 (23%), Positives = 32/73 (43%)
Frame = +1
Query: 232 FIRRYREVDSSQLKHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFDPTKLKHTETCEKN 411
+ R +++ QL+ T+ + K P E +K K D K ++ E C
Sbjct: 402 YARVPKQLCPPQLEETDVKRKRNAPGPSPKEPAMKKQK-----SEMDCDKEENAEDCFGE 456
Query: 412 PLPTKDVIEQEKS 450
P+P + ++E +S
Sbjct: 457 PVPERFIVEMRRS 469
>At3g02320.1 68416.m00214 N2,N2-dimethylguanosine tRNA
methyltransferase family protein similar to
N2,N2-dimethylguanosine tRNA methyltransferase [Homo
sapiens] GI:11066198; contains Pfam profile PF02005:
N2,N2-dimethylguanosine tRNA methyltransferase
Length = 599
Score = 28.3 bits (60), Expect = 3.4
Identities = 17/49 (34%), Positives = 25/49 (51%)
Frame = +1
Query: 226 EVFIRRYREVDSSQLKHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFD 372
E +R +E ++ K T K + +KDA EA KE+ NG+ N D
Sbjct: 45 EFLSKRKQEHEAKSSKRTRPASK--VIEKDASEASKEETPSENGMNNGD 91
>At3g19840.1 68416.m02513 FF domain-containing protein / WW
domain-containing protein weak similarity to
transcription factor CA150b [Mus musculus] GI:6329166;
contains Pfam profiles PF01846: FF domain, PF00397: WW
domain
Length = 743
Score = 27.5 bits (58), Expect = 5.9
Identities = 15/55 (27%), Positives = 25/55 (45%)
Frame = +3
Query: 285 SGEEPASGQRCYRSGEGKEQIPERHRELRSH*AEAHGNVREEPAPHKGRH*AREI 449
S E+ A R + + K + ER + SH ++ ++R EP H RE+
Sbjct: 489 SAEQKAQEIRAAAASDFKTMLREREISINSHWSKVKDSLRNEPRYRSVAHEDREV 543
>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
protein contains Pfam profile PF01480: PWI domain
Length = 878
Score = 27.5 bits (58), Expect = 5.9
Identities = 30/127 (23%), Positives = 49/127 (38%), Gaps = 4/127 (3%)
Frame = +3
Query: 6 LSARIFYPLPHQKYIDSQWPAR*VTLPP*KT----SPRSQQT*RVSSKASTQAVSVTSTP 173
++ R+ P P + + S PA+ + PP + SP ++ + + S S P
Sbjct: 456 VAQRLPSPPPRRAGLPSPPPAQRLPSPPPRRAGLPSPMRIGGSHAANHLESPSPSSLSPP 515
Query: 174 MKRLCFGLLKTSPLRRPRSLYSTVSRS*FEPAEAHRDSGEEPASGQRCYRSGEGKEQIPE 353
++ +L + P+RR RSL R H G G GK
Sbjct: 516 GRKK---VLPSPPVRRRRSLTPDEERVSLSQGGRHTSPSHIKQDGSMSPVRGRGKSSPSS 572
Query: 354 RHRELRS 374
RH++ RS
Sbjct: 573 RHQKARS 579
>At3g54290.1 68416.m06000 expressed protein contains Pfam PF03794:
Domain of Unknown function
Length = 350
Score = 27.1 bits (57), Expect = 7.8
Identities = 14/37 (37%), Positives = 20/37 (54%)
Frame = +1
Query: 241 RYREVDSSQLKHTETQEKNPLPDKDAIEAEKEKNKFL 351
R++ + H E +EK+ LP +A E KEK K L
Sbjct: 260 RFKSLQMMCKTHFEEEEKDLLPMVEAAEMGKEKQKKL 296
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,246,471
Number of Sequences: 28952
Number of extensions: 207963
Number of successful extensions: 721
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 690
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 721
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 977150592
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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