Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1194X
(498 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g21060.1 68415.m02500 cold-shock DNA-binding family protein /... 37 0.009
At4g38680.1 68417.m05477 cold-shock DNA-binding family protein c... 36 0.011
At4g36020.1 68417.m05128 cold-shock DNA-binding family protein c... 34 0.046
At2g17870.1 68415.m02070 cold-shock DNA-binding family protein c... 31 0.43
At2g42960.1 68415.m05328 protein kinase family protein contains ... 28 4.0
At5g59950.3 68418.m07518 RNA and export factor-binding protein, ... 27 5.3
At5g59950.2 68418.m07519 RNA and export factor-binding protein, ... 27 5.3
At5g59950.1 68418.m07517 RNA and export factor-binding protein, ... 27 5.3
At5g50800.1 68418.m06293 nodulin MtN3 family protein similar to ... 27 5.3
At2g33620.3 68415.m04122 DNA-binding family protein / AT-hook pr... 27 5.3
At2g33620.2 68415.m04121 DNA-binding family protein / AT-hook pr... 27 5.3
At2g33620.1 68415.m04120 DNA-binding family protein / AT-hook pr... 27 5.3
At5g48940.1 68418.m06054 leucine-rich repeat transmembrane prote... 27 7.0
At1g56720.2 68414.m06524 protein kinase family protein contains ... 27 7.0
At1g56720.1 68414.m06523 protein kinase family protein contains ... 27 7.0
At2g18510.1 68415.m02157 pre-mRNA splicing factor, putative simi... 27 9.3
>At2g21060.1 68415.m02500 cold-shock DNA-binding family protein /
glycine-rich protein (GRP2) identical to Glycine-rich
protein 2b (AtGRP2b) [Arabidopsis thaliana]
SWISS-PROT:Q38896; contains Pfam domains PF00313:
'Cold-shock' DNA-binding domain and PF00098: Zinc
knuckle
Length = 201
Score = 36.7 bits (81), Expect = 0.009
Identities = 13/29 (44%), Positives = 21/29 (72%)
Frame = +3
Query: 168 EKVSGTVKWFNVKSGYGFINRNDTKEDVF 254
++ GTVKWF+ + G+GFI +D +D+F
Sbjct: 13 DRRKGTVKWFDTQKGFGFITPSDGGDDLF 41
Score = 29.9 bits (64), Expect = 1.00
Identities = 26/78 (33%), Positives = 36/78 (46%), Gaps = 1/78 (1%)
Frame = +2
Query: 254 LHQTAIARNNPRKAVRSVGDGEAVEFAVVAGEKGF-EAAGVTGPGGEPVKGSPYAADKLR 430
+HQ++I + RS+ E+VEF V G +A V+GP G PV+G+
Sbjct: 42 VHQSSIRS----EGFRSLAAEESVEFDVEVDNSGRPKAIEVSGPDGAPVQGNSGGGGSSG 97
Query: 431 GYHRQYFPRQRGGLGGVG 484
G RGG GG G
Sbjct: 98 G---------RGGFGGGG 106
>At4g38680.1 68417.m05477 cold-shock DNA-binding family protein
contains Pfam domains PF00313: 'Cold-shock' DNA-binding
domain and PF00098: Zinc knuckle
Length = 203
Score = 36.3 bits (80), Expect = 0.011
Identities = 13/29 (44%), Positives = 21/29 (72%)
Frame = +3
Query: 168 EKVSGTVKWFNVKSGYGFINRNDTKEDVF 254
E+ G+VKWF+ + G+GFI +D +D+F
Sbjct: 9 ERRKGSVKWFDTQKGFGFITPDDGGDDLF 37
Score = 29.1 bits (62), Expect = 1.7
Identities = 19/52 (36%), Positives = 29/52 (55%), Gaps = 1/52 (1%)
Frame = +2
Query: 254 LHQTAIARNNPRKAVRSVGDGEAVEFAV-VAGEKGFEAAGVTGPGGEPVKGS 406
+HQ++I + RS+ EAVEF V + +A V+GP G PV+G+
Sbjct: 38 VHQSSIRS----EGFRSLAAEEAVEFEVEIDNNNRPKAIDVSGPDGAPVQGN 85
>At4g36020.1 68417.m05128 cold-shock DNA-binding family protein
contains Pfam domains, PF00313: 'Cold-shock' DNA-binding
domain and PF00098: Zinc knuckle
Length = 299
Score = 34.3 bits (75), Expect = 0.046
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = +3
Query: 165 AEKVSGTVKWFNVKSGYGFINRNDTKEDVF 254
A + +G V WFN GYGFI +D ++F
Sbjct: 8 AARSTGKVNWFNASKGYGFITPDDGSVELF 37
Score = 33.9 bits (74), Expect = 0.061
Identities = 20/50 (40%), Positives = 29/50 (58%), Gaps = 1/50 (2%)
Frame = +2
Query: 254 LHQTAIARNNPRKAVRSVGDGEAVEFAVVAGEKG-FEAAGVTGPGGEPVK 400
+HQ++I + RS+ G+AVEFA+ G G +A VT PGG +K
Sbjct: 38 VHQSSIVS----EGYRSLTVGDAVEFAITQGSDGKTKAVNVTAPGGGSLK 83
>At2g17870.1 68415.m02070 cold-shock DNA-binding family protein
contains Pfam domains, PF00313: 'Cold-shock' DNA-binding
domain and PF00098: Zinc knuckle
Length = 301
Score = 31.1 bits (67), Expect = 0.43
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = +3
Query: 165 AEKVSGTVKWFNVKSGYGFINRNDTKEDVF 254
A + G V WF+ GYGFI +D E++F
Sbjct: 8 AARSIGKVSWFSDGKGYGFITPDDGGEELF 37
>At2g42960.1 68415.m05328 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 494
Score = 27.9 bits (59), Expect = 4.0
Identities = 12/30 (40%), Positives = 19/30 (63%), Gaps = 2/30 (6%)
Frame = -2
Query: 494 CVELLHRLV--RHVDEGNIAGGSHGACLQH 411
C+E +HR++ +V+ GN+ HGA QH
Sbjct: 246 CIEGVHRMLVYEYVNSGNLEQWLHGAMRQH 275
>At5g59950.3 68418.m07518 RNA and export factor-binding protein,
putative
Length = 242
Score = 27.5 bits (58), Expect = 5.3
Identities = 18/62 (29%), Positives = 27/62 (43%)
Frame = +2
Query: 311 DGEAVEFAVVAGEKGFEAAGVTGPGGEPVKGSPYAADKLRGYHRQYFPRQRGGLGGVGAP 490
DG+ ++ +V AA P G+P+ + RG ++ R GG GG G
Sbjct: 152 DGKPMKIEIVGTNLQTAAAPSGRPANGNSNGAPWRGGQGRGGQQRGGGRGGGGRGGGGRG 211
Query: 491 RR 496
RR
Sbjct: 212 RR 213
>At5g59950.2 68418.m07519 RNA and export factor-binding protein,
putative
Length = 178
Score = 27.5 bits (58), Expect = 5.3
Identities = 18/62 (29%), Positives = 27/62 (43%)
Frame = +2
Query: 311 DGEAVEFAVVAGEKGFEAAGVTGPGGEPVKGSPYAADKLRGYHRQYFPRQRGGLGGVGAP 490
DG+ ++ +V AA P G+P+ + RG ++ R GG GG G
Sbjct: 88 DGKPMKIEIVGTNLQTAAAPSGRPANGNSNGAPWRGGQGRGGQQRGGGRGGGGRGGGGRG 147
Query: 491 RR 496
RR
Sbjct: 148 RR 149
>At5g59950.1 68418.m07517 RNA and export factor-binding protein,
putative
Length = 244
Score = 27.5 bits (58), Expect = 5.3
Identities = 18/62 (29%), Positives = 27/62 (43%)
Frame = +2
Query: 311 DGEAVEFAVVAGEKGFEAAGVTGPGGEPVKGSPYAADKLRGYHRQYFPRQRGGLGGVGAP 490
DG+ ++ +V AA P G+P+ + RG ++ R GG GG G
Sbjct: 154 DGKPMKIEIVGTNLQTAAAPSGRPANGNSNGAPWRGGQGRGGQQRGGGRGGGGRGGGGRG 213
Query: 491 RR 496
RR
Sbjct: 214 RR 215
>At5g50800.1 68418.m06293 nodulin MtN3 family protein similar to
MtN3 GI:1619602 (root nodule development) from [Medicago
truncatula]
Length = 294
Score = 27.5 bits (58), Expect = 5.3
Identities = 11/24 (45%), Positives = 17/24 (70%)
Frame = +3
Query: 270 SPVTTHVRLCARSATERRWSLPWL 341
+PV T VR+C + +TE SLP++
Sbjct: 26 APVPTFVRICKKKSTEGFQSLPYV 49
>At2g33620.3 68415.m04122 DNA-binding family protein / AT-hook
protein 1 (AHP1) identical to AT-hook protein 1
[Arabidopsis thaliana] gi|2598227|emb|CAA10857
Length = 351
Score = 27.5 bits (58), Expect = 5.3
Identities = 19/51 (37%), Positives = 24/51 (47%)
Frame = +2
Query: 302 SVGDGEAVEFAVVAGEKGFEAAGVTGPGGEPVKGSPYAADKLRGYHRQYFP 454
S G+ + + GE G G+TG G EPVK K RG R+Y P
Sbjct: 69 SAGENSVLNMNLPGGESG----GMTGTGSEPVK-------KRRGRPRKYGP 108
>At2g33620.2 68415.m04121 DNA-binding family protein / AT-hook
protein 1 (AHP1) identical to AT-hook protein 1
[Arabidopsis thaliana] gi|2598227|emb|CAA10857
Length = 351
Score = 27.5 bits (58), Expect = 5.3
Identities = 19/51 (37%), Positives = 24/51 (47%)
Frame = +2
Query: 302 SVGDGEAVEFAVVAGEKGFEAAGVTGPGGEPVKGSPYAADKLRGYHRQYFP 454
S G+ + + GE G G+TG G EPVK K RG R+Y P
Sbjct: 69 SAGENSVLNMNLPGGESG----GMTGTGSEPVK-------KRRGRPRKYGP 108
>At2g33620.1 68415.m04120 DNA-binding family protein / AT-hook
protein 1 (AHP1) identical to AT-hook protein 1
[Arabidopsis thaliana] gi|2598227|emb|CAA10857
Length = 351
Score = 27.5 bits (58), Expect = 5.3
Identities = 19/51 (37%), Positives = 24/51 (47%)
Frame = +2
Query: 302 SVGDGEAVEFAVVAGEKGFEAAGVTGPGGEPVKGSPYAADKLRGYHRQYFP 454
S G+ + + GE G G+TG G EPVK K RG R+Y P
Sbjct: 69 SAGENSVLNMNLPGGESG----GMTGTGSEPVK-------KRRGRPRKYGP 108
>At5g48940.1 68418.m06054 leucine-rich repeat transmembrane protein
kinase, putative
Length = 1135
Score = 27.1 bits (57), Expect = 7.0
Identities = 15/40 (37%), Positives = 24/40 (60%)
Frame = -3
Query: 352 FLPGNHGKLHRLSVADRAHSLTWVVTGDGSLMQNTSSLVS 233
F+P L+RLSV D +H++ ++GD S + +LVS
Sbjct: 627 FIPERISALNRLSVLDISHNM---LSGDLSALSGLENLVS 663
>At1g56720.2 68414.m06524 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 492
Score = 27.1 bits (57), Expect = 7.0
Identities = 12/30 (40%), Positives = 18/30 (60%), Gaps = 2/30 (6%)
Frame = -2
Query: 494 CVELLHRLV--RHVDEGNIAGGSHGACLQH 411
C+E HR++ +V+ GN+ HGA QH
Sbjct: 242 CIEGTHRILVYEYVNNGNLEQWLHGAMRQH 271
>At1g56720.1 68414.m06523 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 492
Score = 27.1 bits (57), Expect = 7.0
Identities = 12/30 (40%), Positives = 18/30 (60%), Gaps = 2/30 (6%)
Frame = -2
Query: 494 CVELLHRLV--RHVDEGNIAGGSHGACLQH 411
C+E HR++ +V+ GN+ HGA QH
Sbjct: 242 CIEGTHRILVYEYVNNGNLEQWLHGAMRQH 271
>At2g18510.1 68415.m02157 pre-mRNA splicing factor, putative similar
to SP|Q15427 Splicing factor 3B subunit 4 (Spliceosome
associated protein 49) (SAP 49) (SF3b50) (Pre-mRNA
splicing factor SF3b 49 kDa subunit) {Homo sapiens};
contains Pfam profile PF00076: RNA recognition motif.
(a.k.a. RRM, RBD, or RNP domain)
Length = 363
Score = 26.6 bits (56), Expect = 9.3
Identities = 13/38 (34%), Positives = 18/38 (47%), Gaps = 2/38 (5%)
Frame = -1
Query: 489 GAPTPPSPPR*RG--KYCRW*PRSLSAA*GEPFTGSPP 382
G P PP PP+ G ++ W P+ + G P PP
Sbjct: 310 GGPRPPPPPQAMGMHQHGGWPPQHMQQQGGPPQQQQPP 347
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,602,833
Number of Sequences: 28952
Number of extensions: 169705
Number of successful extensions: 577
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 555
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 577
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 878448512
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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