Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1187
(752 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 62 8e-11
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 51 2e-07
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 49 8e-07
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 42 1e-04
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 40 5e-04
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 37 0.004
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 36 0.005
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 29 0.71
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 28 1.6
SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9... 27 2.2
SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr 1||... 26 5.0
SPBC32H8.05 |||conserved fungal protein|Schizosaccharomyces pomb... 25 8.8
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 62.1 bits (144), Expect = 8e-11
Identities = 32/86 (37%), Positives = 48/86 (55%), Gaps = 3/86 (3%)
Frame = +1
Query: 256 EESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPI-DYSGGRQADDIISWLKKKTG 432
E+ I L +VD T+E DL Y +RGYPTL F+NG I YSG R+ D ++ +++K+
Sbjct: 69 EKDGISLVEVDCTEEGDLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYMRKQLL 128
Query: 433 PPAVEVT--SAEQAKELIDANTVIVF 504
P ++ + E E D V+ F
Sbjct: 129 PTVKPISKDTLENFVEKADDLAVVAF 154
Score = 61.3 bits (142), Expect = 1e-10
Identities = 25/52 (48%), Positives = 39/52 (75%), Gaps = 1/52 (1%)
Frame = +2
Query: 104 TEENVLVLSKANFETVITT-TEYILVEFYAPWCGHCKSLAPEYAKAATKLAE 256
++E+++VL NF+ ++ T+ +LVEFYAPWCGHCK+LAP Y K A + ++
Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYSD 404
Score = 58.8 bits (136), Expect = 8e-10
Identities = 25/49 (51%), Positives = 36/49 (73%)
Frame = +2
Query: 125 LSKANFETVITTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEKNLLS 271
++K +IT + ++V+FYAPWCGHCK+LAPEY AA +L EK+ +S
Sbjct: 27 VNKEGLNELITADKVLMVKFYAPWCGHCKALAPEYESAADEL-EKDGIS 74
Score = 39.5 bits (88), Expect = 5e-04
Identities = 20/59 (33%), Positives = 38/59 (64%), Gaps = 3/59 (5%)
Frame = +1
Query: 256 EESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNG---SPIDYSGGRQADDIISWLKK 423
++S + +AK+DAT E D++ S + G+PT+ FF+ +P+ Y G R +D+ +++ K
Sbjct: 404 DDSNVVVAKIDAT-ENDISVS--ISGFPTIMFFKANDKVNPVRYEGDRTLEDLSAFIDK 459
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 50.8 bits (116), Expect = 2e-07
Identities = 24/67 (35%), Positives = 31/67 (46%)
Frame = +2
Query: 50 LIFTAIALLGLALGDEVPTEENVLVLSKANFETVITTTEYILVEFYAPWCGHCKSLAPEY 229
L +L+ G N + L+ NF + LV FYAPWCG+CK L P Y
Sbjct: 11 LFLACFSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPWCGYCKKLVPTY 70
Query: 230 AKAATKL 250
K A+ L
Sbjct: 71 QKLASNL 77
Score = 30.3 bits (65), Expect = 0.31
Identities = 19/72 (26%), Positives = 34/72 (47%), Gaps = 6/72 (8%)
Frame = +1
Query: 265 PIKLAKVDATQEQDLAESYGVRGYPTLKFF---RNGSPI---DYSGGRQADDIISWLKKK 426
P+ DA Q + + Y V+G+PT+K GS + DY+G R + ++
Sbjct: 82 PVTAVDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDRSYKSLQKFVSDS 141
Query: 427 TGPPAVEVTSAE 462
P V++ ++E
Sbjct: 142 I-PSKVKILTSE 152
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 48.8 bits (111), Expect = 8e-07
Identities = 24/80 (30%), Positives = 42/80 (52%), Gaps = 2/80 (2%)
Frame = +1
Query: 256 EESPIKLAKVDATQEQDLAESYGVRGYPTLKFF-RNGS-PIDYSGGRQADDIISWLKKKT 429
+ + + + K+DA D+A+ Y + G+PTL +F +GS P+ YS R D + ++ +KT
Sbjct: 70 DHNDVLIGKIDADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNARDVDSLTQFVSEKT 129
Query: 430 GPPAVEVTSAEQAKELIDAN 489
G ++ EL N
Sbjct: 130 GIKKRKIVLPSNVVELDSLN 149
Score = 48.8 bits (111), Expect = 8e-07
Identities = 21/40 (52%), Positives = 27/40 (67%), Gaps = 1/40 (2%)
Frame = +2
Query: 113 NVLVLSKANFETVITTTEY-ILVEFYAPWCGHCKSLAPEY 229
NV+ L NF+ V+ + +LVEFYA WCG+CK LAP Y
Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTY 180
Score = 47.6 bits (108), Expect = 2e-06
Identities = 25/76 (32%), Positives = 37/76 (48%)
Frame = +2
Query: 35 IEMRVLIFTAIALLGLALGDEVPTEENVLVLSKANFETVITTTEYILVEFYAPWCGHCKS 214
+ + +L F AL L V +++ L T+ + + L+EFYA WCGHCKS
Sbjct: 1 MRLPLLSFVIFALFALVFASGVVELQSLNELEN----TIRASKKGALIEFYATWCGHCKS 56
Query: 215 LAPEYAKAATKLAEKN 262
LAP Y + + N
Sbjct: 57 LAPVYEELGALFEDHN 72
Score = 37.9 bits (84), Expect = 0.002
Identities = 18/61 (29%), Positives = 32/61 (52%), Gaps = 3/61 (4%)
Frame = +1
Query: 259 ESPIKLAKVDATQEQDLAESYGVRGYPTLKFF---RNGSPIDYSGGRQADDIISWLKKKT 429
E +++ K++A D+ + V +PT+KFF P Y G R + +I ++ KK+
Sbjct: 190 EPNVEIVKINADVFADIGRLHEVASFPTIKFFPKDDKDKPELYEGDRSLESLIEYINKKS 249
Query: 430 G 432
G
Sbjct: 250 G 250
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 41.5 bits (93), Expect = 1e-04
Identities = 12/49 (24%), Positives = 31/49 (63%)
Frame = +2
Query: 122 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEKNLL 268
V + F++++ + ++V+F+A WCG CK++AP++ + + ++ +
Sbjct: 5 VSDSSEFKSIVCQDKLVVVDFFATWCGPCKAIAPKFEQFSNTYSDATFI 53
Score = 27.1 bits (57), Expect = 2.9
Identities = 11/32 (34%), Positives = 17/32 (53%)
Frame = +1
Query: 280 KVDATQEQDLAESYGVRGYPTLKFFRNGSPID 375
KVD Q ++A GV P+ ++NG I+
Sbjct: 54 KVDVDQLSEIAAEAGVHAMPSFFLYKNGEKIE 85
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 39.5 bits (88), Expect = 5e-04
Identities = 15/44 (34%), Positives = 29/44 (65%)
Frame = +2
Query: 122 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAPEYAKAATKLA 253
+ S ++ + I + Y+ V+ YA WCG CK+++P +++ A+K A
Sbjct: 6 IRSYQHWISTIPKSGYLAVDCYADWCGPCKAISPLFSQLASKYA 49
Score = 35.9 bits (79), Expect = 0.006
Identities = 23/69 (33%), Positives = 34/69 (49%), Gaps = 4/69 (5%)
Frame = +1
Query: 277 AKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGR--QA--DDIISWLKKKTGPPAV 444
AKV+ +++ +A GV+ PT FF NG ID G QA + + K TG A+
Sbjct: 56 AKVNVDEQRQIASGLGVKAMPTFVFFENGKQIDMLTGANPQALKEKVALISSKATGTGAL 115
Query: 445 EVTSAEQAK 471
+S+ K
Sbjct: 116 ASSSSAPVK 124
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 36.7 bits (81), Expect = 0.004
Identities = 19/47 (40%), Positives = 27/47 (57%)
Frame = +2
Query: 122 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEKN 262
V S ++ T I+ + +V+FYA WCG CK L P KL+E+N
Sbjct: 22 VESFGDYNTRISADKVTVVDFYADWCGPCKYLKP----FLEKLSEQN 64
Score = 31.1 bits (67), Expect = 0.18
Identities = 16/47 (34%), Positives = 23/47 (48%)
Frame = +1
Query: 283 VDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKK 423
V+A + D+A+ GV PT+ FR G +D G + S L K
Sbjct: 72 VNADKFSDIAQKNGVYALPTMVLFRKGQELDRIVGADVKTLSSLLAK 118
>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 36.3 bits (80), Expect = 0.005
Identities = 12/65 (18%), Positives = 36/65 (55%)
Frame = +1
Query: 268 IKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKKKTGPPAVE 447
+K+A+V+ +E+++ + ++ +PT + F+ I Y+G + ++S+ + A++
Sbjct: 230 LKMAQVNCDEEKEMCNHFHIKKFPTFRVFQGFDSIQYNGPLKYQQLLSYSNQVASYQAIK 289
Query: 448 VTSAE 462
+ +
Sbjct: 290 IEEGD 294
Score = 28.3 bits (60), Expect = 1.2
Identities = 18/54 (33%), Positives = 25/54 (46%), Gaps = 5/54 (9%)
Frame = +1
Query: 307 LAESYGVRGYPTLKFFRNGSPIDYSG--GRQADD---IISWLKKKTGPPAVEVT 453
LA YG + P++ RNG PI Y R+ D I W+ + P E+T
Sbjct: 343 LANKYGAQSQPSIIAVRNGMPIVYQAITPREFRDYKRITEWINIVSSPFITELT 396
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 29.1 bits (62), Expect = 0.71
Identities = 10/52 (19%), Positives = 28/52 (53%)
Frame = +1
Query: 268 IKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKK 423
+ +A ++ + + Y ++ +PT FF+ + ++Y G D++S+ ++
Sbjct: 331 LNVAHINCAVSKRACKQYSIQYFPTFLFFKEEAFVEYVGLPNEGDLVSFAEE 382
Score = 27.9 bits (59), Expect = 1.6
Identities = 10/28 (35%), Positives = 15/28 (53%)
Frame = +2
Query: 176 VEFYAPWCGHCKSLAPEYAKAATKLAEK 259
+++Y P CG CK L P + K E+
Sbjct: 47 IKYYLPSCGACKRLGPMWDNMVEKAKEQ 74
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 27.9 bits (59), Expect = 1.6
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +2
Query: 164 EYILVEFYAPWCGHCKSL 217
+ IL+ FYAPW CK +
Sbjct: 21 QIILLNFYAPWAAPCKQM 38
>SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit
Srb9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1223
Score = 27.5 bits (58), Expect = 2.2
Identities = 14/41 (34%), Positives = 19/41 (46%)
Frame = +3
Query: 111 KMCSF*VKLTLKL*LQPRSTF*LNSMLHGAATANLWHRNTP 233
K +F L LQP T N +L+ + NLW R+ P
Sbjct: 620 KSANFDFSFLKSLDLQPTITLGKNDLLNAILSQNLWFRSLP 660
>SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1367
Score = 26.2 bits (55), Expect = 5.0
Identities = 13/45 (28%), Positives = 22/45 (48%)
Frame = +3
Query: 207 ANLWHRNTPRQQQSWLRRISYQTSES*RNSRTGSRRELRCTRIPD 341
AN + + QSW R++ + +E+ S S R + T IP+
Sbjct: 74 ANSFEGSCNNSDQSWTSRVTSKKNEAGTESGDASVRRIYVTSIPE 118
>SPBC32H8.05 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 117
Score = 25.4 bits (53), Expect = 8.8
Identities = 20/60 (33%), Positives = 28/60 (46%), Gaps = 2/60 (3%)
Frame = +3
Query: 525 SARAKTFLSTAQVVDDQVFAIVSDEKVIKELEAEDEDVV--LFKNFEEKRVKYEDEEITE 698
SAR+K+ +V+ + VF V DE+ K L A D V L K+ K D + E
Sbjct: 4 SARSKSIRRNKKVLRENVFQPVIDERT-KRLSAHLRDQVNDLTKSSSSKEEGIADNSLKE 62
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,825,294
Number of Sequences: 5004
Number of extensions: 54338
Number of successful extensions: 180
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 162
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 179
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 359287726
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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