BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1151
(738 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1020.01c |pma2|SPCC1393.01|P-type proton ATPase Pma2 |Schizo... 32 0.074
SPAC1071.10c |pma1||P-type proton ATPase Pma1 |Schizosaccharomyc... 32 0.074
SPAPB1E7.10 |rpc17||DNA-directed RNA polymerase III complex subu... 27 3.7
SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1 |Schiz... 26 4.9
SPCC594.05c |||COMPASS complex subunit |Schizosaccharomyces pomb... 26 6.4
SPAC25B8.14 |mal2||kinetochore protein Mal2 |Schizosaccharomyces... 25 8.5
>SPCC1020.01c |pma2|SPCC1393.01|P-type proton ATPase Pma2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1010
Score = 32.3 bits (70), Expect = 0.074
Identities = 20/75 (26%), Positives = 37/75 (49%), Gaps = 2/75 (2%)
Frame = +1
Query: 367 RYSFNVLYFFVTTEVVRRLQINDSVNNFFDCLN--LTIELYVFVLSMYVDLDTVFNAFDL 540
R F+ +Y +V + L + + + N L +EL VF+ +++ D+ T+ A+D
Sbjct: 768 RQIFHRMYAYVVYRIALSLHLEIFLGLWLIIRNQLLNLELIVFI-AIFADVATLAIAYDN 826
Query: 541 TPYPVLPTHKNKPNI 585
PY + P N P +
Sbjct: 827 APYAMKPVKWNLPRL 841
>SPAC1071.10c |pma1||P-type proton ATPase Pma1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 919
Score = 32.3 bits (70), Expect = 0.074
Identities = 20/75 (26%), Positives = 37/75 (49%), Gaps = 2/75 (2%)
Frame = +1
Query: 367 RYSFNVLYFFVTTEVVRRLQINDSVNNFFDCLN--LTIELYVFVLSMYVDLDTVFNAFDL 540
R F+ +Y +V + L + + + N L +EL VF+ +++ D+ T+ A+D
Sbjct: 680 RQIFHRMYSYVVYRIALSLHLEIFLGLWLIIRNQLLNLELVVFI-AIFADVATLAIAYDN 738
Query: 541 TPYPVLPTHKNKPNI 585
PY + P N P +
Sbjct: 739 APYSMKPVKWNLPRL 753
>SPAPB1E7.10 |rpc17||DNA-directed RNA polymerase III complex subunit
Rpc17|Schizosaccharomyces pombe|chr 1|||Manual
Length = 129
Score = 26.6 bits (56), Expect = 3.7
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = +1
Query: 502 YVDLDTVFNAFDLTPYPVLPTHKNKPNISP 591
++D +FN F+LT +L NKP+ P
Sbjct: 70 FLDCIAIFNEFELTKAEILVILNNKPSSVP 99
>SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 676
Score = 26.2 bits (55), Expect = 4.9
Identities = 12/32 (37%), Positives = 21/32 (65%), Gaps = 1/32 (3%)
Frame = -1
Query: 462 KTIKKIVYG-IIYLQPSHYFSRYEKIQNIETV 370
K ++++VY +I + SH+F+ E IQN+ V
Sbjct: 609 KKVRQLVYDDLIRIGRSHHFANIELIQNVVLV 640
>SPCC594.05c |||COMPASS complex subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 424
Score = 25.8 bits (54), Expect = 6.4
Identities = 14/49 (28%), Positives = 23/49 (46%)
Frame = +2
Query: 545 RTRCCQHTKTNLTYPR*VYIFNSNFTNKPNLDVLDKYLSLRH*KKKFCL 691
+ RC +HT + + + SN K N+ K + L H +K+ CL
Sbjct: 351 KRRCAKHTSWQVIFTEDFELQESNILQKLNMKQTAKDVMLEH-QKQRCL 398
>SPAC25B8.14 |mal2||kinetochore protein Mal2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 303
Score = 25.4 bits (53), Expect = 8.5
Identities = 15/48 (31%), Positives = 25/48 (52%), Gaps = 3/48 (6%)
Frame = -2
Query: 554 TGYGVKSNALNTVSKSTYIDNTNTYSSIV---RLRQSKKLFTESFICS 420
T Y + NAL ++KS ID TN +++ + Q K+ + +CS
Sbjct: 204 TAYICRKNALLQINKSLTIDGTNISANLSCTHLVIQIPKVIQATLVCS 251
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,586,086
Number of Sequences: 5004
Number of extensions: 49166
Number of successful extensions: 123
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 121
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 123
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 349251756
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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