BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1148
(341 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23D3.14c |aah2||alpha-amylase homolog Aah2|Schizosaccharomyc... 29 0.15
SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pomb... 26 1.8
SPCC70.09c |mug9||conserved fungal protein|Schizosaccharomyces p... 26 1.8
SPAC20H4.06c |||RNA-binding protein|Schizosaccharomyces pombe|ch... 25 2.4
SPAC637.08 |||iron-sulfur cluster assembly ATPase Nbp35|Schizosa... 25 4.3
SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyce... 25 4.3
SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccha... 25 4.3
SPAC17H9.02 |||ATP-dependent RNA helicase Mtr4-like |Schizosacch... 24 5.6
SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosacc... 24 5.6
SPAC56F8.12 |||conserved fungal protein|Schizosaccharomyces pomb... 24 7.5
SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy... 24 7.5
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 24 7.5
SPAC22E12.11c |set3||histone lysine methyltransferase Set3|Schiz... 23 9.8
SPBC4B4.04 |||translation initiation factor eIF2A |Schizosacchar... 23 9.8
>SPAC23D3.14c |aah2||alpha-amylase homolog Aah2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 581
Score = 29.5 bits (63), Expect = 0.15
Identities = 13/29 (44%), Positives = 19/29 (65%), Gaps = 1/29 (3%)
Frame = -1
Query: 287 AISLTSTHEECNKINTRYTFL-IHSVPRV 204
AI++ +EECN IN TF+ H +PR+
Sbjct: 301 AIAINQINEECNDINVLGTFIGNHDLPRI 329
>SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1647
Score = 25.8 bits (54), Expect = 1.8
Identities = 12/36 (33%), Positives = 19/36 (52%), Gaps = 1/36 (2%)
Frame = +2
Query: 80 IAPRT-PAATKNERAVPITQTGPVRKEIKEPTAEVE 184
+AP + PA +K P+T GP+ I+ T +E
Sbjct: 675 LAPMSIPAISKRSLTKPVTSCGPLLNNIRRLTVNLE 710
>SPCC70.09c |mug9||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 208
Score = 25.8 bits (54), Expect = 1.8
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = +2
Query: 89 RTPAATKNERAVPITQTGPVRKEIKE 166
R PAA + + VPITQ + E+K+
Sbjct: 172 RRPAAMRAQTLVPITQPDSKKNELKQ 197
>SPAC20H4.06c |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 2.4
Identities = 7/18 (38%), Positives = 13/18 (72%)
Frame = -1
Query: 116 ARSSWQPESWERSRSARR 63
++ WQP+SW+ SR+ +
Sbjct: 70 SKEGWQPKSWKSSRNENK 87
>SPAC637.08 |||iron-sulfur cluster assembly ATPase
Nbp35|Schizosaccharomyces pombe|chr 1|||Manual
Length = 317
Score = 24.6 bits (51), Expect = 4.3
Identities = 11/34 (32%), Positives = 18/34 (52%)
Frame = -3
Query: 111 FFVAAGVLGAITVSTPSEDSRDSTVSGLQFSGRA 10
FF +G+ GA+ V+TP E + + F +A
Sbjct: 192 FFKNSGIDGAVVVTTPQEVALQDVRKEIDFCRKA 225
>SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2310
Score = 24.6 bits (51), Expect = 4.3
Identities = 14/34 (41%), Positives = 18/34 (52%), Gaps = 2/34 (5%)
Frame = -3
Query: 231 FFDSQRSESHCFVSLLSTSAVGSLIS--FRTGPV 136
F+D ES VSL+S G L+S R GP+
Sbjct: 1421 FYDRNDEESFDIVSLVSVIKCGQLLSSKLRLGPL 1454
>SPAC16C9.06c |upf1||ATP-dependent RNA helicase
Upf1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 925
Score = 24.6 bits (51), Expect = 4.3
Identities = 13/37 (35%), Positives = 19/37 (51%), Gaps = 3/37 (8%)
Frame = -1
Query: 281 SLTSTHEECNKINTRYTFLIHSVP---RVIVLFRCSQ 180
SL+ T EC TR FL+ +P + +V+ C Q
Sbjct: 96 SLSDTVLECYNCGTRNVFLLGFIPAKAKTVVVLLCRQ 132
>SPAC17H9.02 |||ATP-dependent RNA helicase Mtr4-like
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1030
Score = 24.2 bits (50), Expect = 5.6
Identities = 13/42 (30%), Positives = 23/42 (54%)
Frame = +3
Query: 186 ATKQNNDSRNAVNQKSVACVNFIAFLVRACETDRFIVFIFNK 311
+T+Q N S+++ K + + I +V + D IVF F+K
Sbjct: 339 STRQENYSKSSKKVKKSSSLERIINMVLSNRYDPIIVFCFSK 380
>SPAC1002.12c |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 498
Score = 24.2 bits (50), Expect = 5.6
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -3
Query: 105 VAAGVLGAITVSTPSEDSRDSTVSGL 28
V AGVL +T ++PSE + T + L
Sbjct: 214 VPAGVLNMVTANSPSEHGIELTTNPL 239
>SPAC56F8.12 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 394
Score = 23.8 bits (49), Expect = 7.5
Identities = 10/15 (66%), Positives = 11/15 (73%)
Frame = +2
Query: 140 GPVRKEIKEPTAEVE 184
GP KE+KE T EVE
Sbjct: 369 GPCEKEMKEETNEVE 383
>SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1583
Score = 23.8 bits (49), Expect = 7.5
Identities = 10/34 (29%), Positives = 20/34 (58%)
Frame = +2
Query: 11 ALPENCNPDTVESRLSSDGVLTVIAPRTPAATKN 112
+LPE C +T+ + + + V+TV + ++KN
Sbjct: 598 SLPEWCGTETIIRQFAMNLVMTVTNDKQAVSSKN 631
>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 690
Score = 23.8 bits (49), Expect = 7.5
Identities = 12/37 (32%), Positives = 19/37 (51%)
Frame = +2
Query: 92 TPAATKNERAVPITQTGPVRKEIKEPTAEVESNETKQ 202
TP++ N AVP++ T V + +PT + S Q
Sbjct: 254 TPSSINNHTAVPLSPTLAVWLPMTQPTFQPPSANVYQ 290
>SPAC22E12.11c |set3||histone lysine methyltransferase
Set3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 859
Score = 23.4 bits (48), Expect = 9.8
Identities = 10/35 (28%), Positives = 18/35 (51%)
Frame = +3
Query: 117 ELFPSLKPARSGRRLRSPLRKLRATKQNNDSRNAV 221
+L PS+K + + L+SPL + N D + +
Sbjct: 480 KLQPSVKEKKPTKDLQSPLPSVEEDSSNRDKKTDI 514
>SPBC4B4.04 |||translation initiation factor eIF2A
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 576
Score = 23.4 bits (48), Expect = 9.8
Identities = 15/41 (36%), Positives = 23/41 (56%)
Frame = +2
Query: 77 VIAPRTPAATKNERAVPITQTGPVRKEIKEPTAEVESNETK 199
V+ TP N++ +T P K++ +PTAEV S+E K
Sbjct: 488 VVPGATPVIDGNKKNNK--KTKPAVKQVAQPTAEV-SDEKK 525
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,271,332
Number of Sequences: 5004
Number of extensions: 22583
Number of successful extensions: 88
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 87
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 88
length of database: 2,362,478
effective HSP length: 64
effective length of database: 2,042,222
effective search space used: 100068878
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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