Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1144
(675 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g13950.1 68414.m01639 eukaryotic translation initiation facto... 99 1e-21
At1g69410.1 68414.m07972 eukaryotic translation initiation facto... 97 7e-21
At1g26630.1 68414.m03243 eukaryotic translation initiation facto... 96 2e-20
At5g28080.1 68418.m03391 protein kinase family protein contains ... 34 0.100
At3g22420.1 68416.m02829 protein kinase family protein contains ... 32 0.40
At3g04910.1 68416.m00533 protein kinase family protein contains ... 31 0.70
At4g10120.1 68417.m01655 sucrose-phosphate synthase, putative si... 30 1.2
At5g03140.1 68418.m00262 lectin protein kinase family protein co... 29 3.8
At3g18750.1 68416.m02380 protein kinase family protein contains ... 29 3.8
At1g49160.2 68414.m05512 protein kinase family protein contains ... 29 3.8
At1g49160.1 68414.m05511 protein kinase family protein contains ... 29 3.8
At5g05980.1 68418.m00662 dihydrofolate synthetase/folylpolygluta... 28 5.0
At4g21700.1 68417.m03142 hypothetical protein 28 5.0
At3g51290.1 68416.m05614 proline-rich family protein 28 5.0
At1g77670.1 68414.m09043 aminotransferase class I and II family ... 28 5.0
At3g49180.1 68416.m05375 transducin family protein / WD-40 repea... 28 6.6
At2g28550.1 68415.m03468 AP2 domain-containing transcription fac... 27 8.7
At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein... 27 8.7
At1g35830.1 68414.m04452 VQ motif-containing protein contains PF... 27 8.7
>At1g13950.1 68414.m01639 eukaryotic translation initiation factor
5A-1 / eIF-5A 1 identical to SP|Q9XI91 Eukaryotic
translation initiation factor 5A-1 (eIF-5A 1)
{Arabidopsis thaliana}
Length = 158
Score = 99 bits (238), Expect = 1e-21
Identities = 45/88 (51%), Positives = 62/88 (70%), Gaps = 1/88 (1%)
Frame = +1
Query: 250 QNGKHGHAKFTWLGLISSMVKSMXYICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADN 429
+ GKHGHAK ++ + K + I PS+HN DVPHV R DYQL DIS+DGY++L+ DN
Sbjct: 48 KTGKHGHAKCHFVAIDIFTSKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDN 107
Query: 430 GDLREDLKIPDGD-LGTQLRTDFDSGKD 510
G ++DLK+P+ D L Q+++ FD GKD
Sbjct: 108 GSTKDDLKLPNDDTLLQQIKSGFDDGKD 135
Score = 70.5 bits (165), Expect = 9e-13
Identities = 27/46 (58%), Positives = 38/46 (82%)
Frame = +2
Query: 119 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKT 256
E+ HFE+ D+GAS T+P Q +RKNG++++K RPCK+VE+STSKT
Sbjct: 4 EEHHFESSDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKT 49
>At1g69410.1 68414.m07972 eukaryotic translation initiation factor
5A, putative / eIF-5A, putative strong similarity to
eukaryotic initiation factor 5A (2) (Nicotiana
plumbaginifolia) GI:19702, SP|Q9AXQ6| Eukaryotic
translation initiation factor 5A-1 (eIF-5A 1)
{Lycopersicon esculentum}
Length = 158
Score = 97.5 bits (232), Expect = 7e-21
Identities = 45/88 (51%), Positives = 61/88 (69%), Gaps = 1/88 (1%)
Frame = +1
Query: 250 QNGKHGHAKFTWLGLISSMVKSMXYICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADN 429
+ GKHGHAK ++ + K + I PS+HN DVPHV R DYQL DIS+DG+++L+ DN
Sbjct: 48 KTGKHGHAKCHFVAIDIFTSKKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDN 107
Query: 430 GDLREDLKIP-DGDLGTQLRTDFDSGKD 510
G ++DLK+P D L TQL+ F+ GKD
Sbjct: 108 GSTKDDLKLPTDEALLTQLKNGFEEGKD 135
Score = 66.9 bits (156), Expect = 1e-11
Identities = 26/46 (56%), Positives = 37/46 (80%)
Frame = +2
Query: 119 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKT 256
++ HFE+ D+GAS T+P Q +RK G +++KGRPCK+VE+STSKT
Sbjct: 4 DEHHFESSDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKT 49
>At1g26630.1 68414.m03243 eukaryotic translation initiation factor
5A, putative / eIF-5A, putative strong similariy to
SP|Q9AXQ6 Eukaryotic translation initiation factor 5A-1
(eIF-5A 1) {Lycopersicon esculentum}
Length = 159
Score = 95.9 bits (228), Expect = 2e-20
Identities = 43/88 (48%), Positives = 60/88 (68%), Gaps = 1/88 (1%)
Frame = +1
Query: 250 QNGKHGHAKFTWLGLISSMVKSMXYICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADN 429
+ GKHGHAK ++ + K + I PS+HN DVPHV R DYQL DI++DG+++L+ D+
Sbjct: 48 KTGKHGHAKCHFVAIDIFTAKKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDS 107
Query: 430 GDLREDLKIP-DGDLGTQLRTDFDSGKD 510
G ++DLK+P D L Q+R FD GKD
Sbjct: 108 GGTKDDLKLPTDDGLTAQMRLGFDEGKD 135
Score = 61.7 bits (143), Expect = 4e-10
Identities = 24/46 (52%), Positives = 34/46 (73%)
Frame = +2
Query: 119 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKT 256
++ HFE +SGAS T+P +RK G +++K RPCK+VE+STSKT
Sbjct: 4 DEHHFEASESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKT 49
>At5g28080.1 68418.m03391 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 406
Score = 33.9 bits (74), Expect = 0.100
Identities = 23/63 (36%), Positives = 28/63 (44%), Gaps = 1/63 (1%)
Frame = +1
Query: 232 C*NVHIQNGKHGHAKFTWLGLISSMVKSMXYICPSTHNMDVPHVKREDY-QLTDISDDGY 408
C N+ I NG G K LGL + + S C T P V +E+Y QL DI G
Sbjct: 70 CDNIFI-NGNQGEVKIGDLGLAACLQHSHAAHCVGTPEFMAPEVYKEEYNQLVDIYSFGM 128
Query: 409 LTL 417
L
Sbjct: 129 CVL 131
>At3g22420.1 68416.m02829 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 568
Score = 31.9 bits (69), Expect = 0.40
Identities = 22/63 (34%), Positives = 28/63 (44%), Gaps = 1/63 (1%)
Frame = +1
Query: 232 C*NVHIQNGKHGHAKFTWLGLISSMVKSMXYICPSTHNMDVPHVKREDY-QLTDISDDGY 408
C N+ I NG G K LGL + + KS C T P V E+Y +L D+ G
Sbjct: 155 CDNIFI-NGNQGEVKIGDLGLAAILRKSHAVRCVGTPEFMAPEVYDEEYNELVDVYAFGM 213
Query: 409 LTL 417
L
Sbjct: 214 CVL 216
>At3g04910.1 68416.m00533 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 700
Score = 31.1 bits (67), Expect = 0.70
Identities = 22/63 (34%), Positives = 27/63 (42%), Gaps = 1/63 (1%)
Frame = +1
Query: 232 C*NVHIQNGKHGHAKFTWLGLISSMVKSMXYICPSTHNMDVPHVKREDY-QLTDISDDGY 408
C N+ + NG G K LGL + + KS C T P V E Y +L DI G
Sbjct: 155 CDNIFV-NGNQGEVKIGDLGLAAILRKSHAAHCVGTPEFMAPEVYEEAYNELVDIYSFGM 213
Query: 409 LTL 417
L
Sbjct: 214 CIL 216
>At4g10120.1 68417.m01655 sucrose-phosphate synthase, putative
similar to sucrose-phosphate synthase, Zea mays,
PIR2:JQ1329; contains non-consensus (GC) donor splice
site at intron 4
Length = 1050
Score = 30.3 bits (65), Expect = 1.2
Identities = 17/52 (32%), Positives = 28/52 (53%)
Frame = +1
Query: 436 LREDLKIPDGDLGTQLRTDFDSGKDCCAPC*NPAVRSASSPSKQTQLSTNKP 591
L +D + PDGDL + + D + K P + +R S+P K T L+ ++P
Sbjct: 461 LTQDSQEPDGDLKSLIGPDRNQIKKPVPPIWSEIMRFFSNPHKPTILALSRP 512
>At5g03140.1 68418.m00262 lectin protein kinase family protein
contains Pfam domains, PF00138: Legume lectins alpha
domain, PF00139: Legume lectins beta domain and PF00069:
Protein kinase domain
Length = 711
Score = 28.7 bits (61), Expect = 3.8
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = -1
Query: 219 RPFSITKPFLRRAEHCMGKVAEAPESPVSKCVSSMS 112
+P +T+P +R + A+ PE P++K SSMS
Sbjct: 631 QPDPVTRPTMRSVVQILVGEADVPEVPIAKPSSSMS 666
>At3g18750.1 68416.m02380 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 567
Score = 28.7 bits (61), Expect = 3.8
Identities = 21/63 (33%), Positives = 28/63 (44%), Gaps = 1/63 (1%)
Frame = +1
Query: 232 C*NVHIQNGKHGHAKFTWLGLISSMVKSMXYICPSTHNMDVPHVKREDY-QLTDISDDGY 408
C N+ I NG HG K LGL + M ++ T P + E+Y +L DI G
Sbjct: 159 CDNIFI-NGNHGEVKIGDLGLATVMEQANAKSVIGTPEFMAPELYDENYNELADIYSFGM 217
Query: 409 LTL 417
L
Sbjct: 218 CML 220
>At1g49160.2 68414.m05512 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 557
Score = 28.7 bits (61), Expect = 3.8
Identities = 21/63 (33%), Positives = 28/63 (44%), Gaps = 1/63 (1%)
Frame = +1
Query: 232 C*NVHIQNGKHGHAKFTWLGLISSMVKSMXYICPSTHNMDVPHVKREDY-QLTDISDDGY 408
C N+ I NG HG K LGL + M ++ T P + E+Y +L DI G
Sbjct: 159 CDNIFI-NGNHGEVKIGDLGLATVMEQANAKSVIGTPEFMAPELYDENYNELADIYSFGM 217
Query: 409 LTL 417
L
Sbjct: 218 CML 220
>At1g49160.1 68414.m05511 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 539
Score = 28.7 bits (61), Expect = 3.8
Identities = 21/63 (33%), Positives = 28/63 (44%), Gaps = 1/63 (1%)
Frame = +1
Query: 232 C*NVHIQNGKHGHAKFTWLGLISSMVKSMXYICPSTHNMDVPHVKREDY-QLTDISDDGY 408
C N+ I NG HG K LGL + M ++ T P + E+Y +L DI G
Sbjct: 141 CDNIFI-NGNHGEVKIGDLGLATVMEQANAKSVIGTPEFMAPELYDENYNELADIYSFGM 199
Query: 409 LTL 417
L
Sbjct: 200 CML 202
>At5g05980.1 68418.m00662 dihydrofolate
synthetase/folylpolyglutamate synthetase (DHFS/FPGS2)
nearly identical to gi:17976705; identical to cDNA
dihydrofolate synthetase/folylpolyglutamate synthetase
(dhfs/fpgs2 gene) GI:17976704
Length = 571
Score = 28.3 bits (60), Expect = 5.0
Identities = 18/70 (25%), Positives = 28/70 (40%)
Frame = +1
Query: 157 SHLPHAMFGPA*KWFRYAEGSSMQDC*NVHIQNGKHGHAKFTWLGLISSMVKSMXYICPS 336
+H P +M A KWF A Q + H+ NG G + W + +
Sbjct: 387 AHSPESMEACA-KWFSVAVKGDNQSGSSGHLVNGSAGSSHDKWSNETCEQILLFNCMSVR 445
Query: 337 THNMDVPHVK 366
N+ +PH+K
Sbjct: 446 DPNLLLPHLK 455
>At4g21700.1 68417.m03142 hypothetical protein
Length = 962
Score = 28.3 bits (60), Expect = 5.0
Identities = 17/59 (28%), Positives = 25/59 (42%)
Frame = -1
Query: 294 QSQPSEFSVAVLSVLDVDISTILHGRPFSITKPFLRRAEHCMGKVAEAPESPVSKCVSS 118
QS PS F + L+ ++ P S P + ++HC V E+P P V S
Sbjct: 21 QSYPSLFFLLFLTTSATSLTVASLVNPHSFIAPRIPYSDHCNHIVPESPIDPSPSAVFS 79
>At3g51290.1 68416.m05614 proline-rich family protein
Length = 602
Score = 28.3 bits (60), Expect = 5.0
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = +3
Query: 96 KTQQWVTSKTHTLRPETPGPQPPSP 170
+T W T+ T ++ P P P PP P
Sbjct: 91 ETTTWTTTTTSSVLPPPPPPPPPPP 115
>At1g77670.1 68414.m09043 aminotransferase class I and II family
protein similar to kynurenine aminotransferase
/glutamine transaminase K GI:1030066 [Rattus norvegicus]
Length = 440
Score = 28.3 bits (60), Expect = 5.0
Identities = 14/31 (45%), Positives = 16/31 (51%)
Frame = +2
Query: 257 ESTATLNSLGWD*YLQW*KV*XISVPPHTTW 349
E T T+NSLG L K+ PPH TW
Sbjct: 274 ERTVTMNSLGKTFSLTGWKIGWAIAPPHLTW 304
>At3g49180.1 68416.m05375 transducin family protein / WD-40 repeat
family protein contains 5 WD-40 repeats (PF00400);
GTP-binding protein beta chain homolog, Nicotiana
tabacum, PIR:T16970
Length = 438
Score = 27.9 bits (59), Expect = 6.6
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = -2
Query: 575 SCVCFDGDDALLTAGFQHGA 516
+C+ F GDD+LL +G Q G+
Sbjct: 124 TCLVFSGDDSLLVSGSQDGS 143
>At2g28550.1 68415.m03468 AP2 domain-containing transcription factor
RAP2.7 (RAP2.7) nearly identical to AP2 domain
transcription factor RAP2.7 (GI:2281639) [Arabidopsis
thaliana]
Length = 449
Score = 27.5 bits (58), Expect = 8.7
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = +3
Query: 120 KTHTLRPETPGPQPP 164
+T +RP+ PGPQPP
Sbjct: 402 QTSAVRPQPPGPQPP 416
>At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein
contains INTERPRO domain, IPR001878: Zn-finger CCHC type
Length = 353
Score = 27.5 bits (58), Expect = 8.7
Identities = 15/45 (33%), Positives = 18/45 (40%), Gaps = 3/45 (6%)
Frame = +3
Query: 120 KTHTLRPETPGPQPPS---PCNVRPCVKMVSLC*RVVHARLLKCP 245
KT T P PP PCN PC + S + + R CP
Sbjct: 22 KTTTKPTAAAAPSPPDIHCPCNAGPCNTVTSKTEKNPNRRFYTCP 66
>At1g35830.1 68414.m04452 VQ motif-containing protein contains
PF05678: VQ motif
Length = 302
Score = 27.5 bits (58), Expect = 8.7
Identities = 11/33 (33%), Positives = 17/33 (51%)
Frame = +3
Query: 72 SFLTVVKFKTQQWVTSKTHTLRPETPGPQPPSP 170
++L V + ++TH L P+ P P PP P
Sbjct: 60 TYLNFVNNLISDDILNQTHLLPPQPPPPPPPPP 92
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,933,623
Number of Sequences: 28952
Number of extensions: 298057
Number of successful extensions: 871
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 803
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 864
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1432596384
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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