Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--1077
         (637 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC18H10.03 |tif35||translation initiation factor eIF3g|Schizos...    45   1e-05
SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|...    31   0.14 
SPBC16E9.01c |php4|SPBP16F5.09c|CCAAT-binding factor complex sub...    27   3.0  
SPCC553.10 |||conserved fungal protein|Schizosaccharomyces pombe...    25   6.9  
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces...    25   9.1  

>SPBC18H10.03 |tif35||translation initiation factor
           eIF3g|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 282

 Score = 44.8 bits (101), Expect = 1e-05
 Identities = 26/77 (33%), Positives = 41/77 (53%), Gaps = 5/77 (6%)
 Frame = +3

Query: 264 VSKSIAKRKTWSKFGDSASDKPGPNPATTNVAEDVFMQ----FITSKEESQRPDDGELDG 431
           V  ++A+RK W KFG  A    G +  TT+V E+V ++    + T+KEE Q  D+  L  
Sbjct: 61  VQHAVAERKKWKKFGKEAGKNSGVDARTTSVGENVQLRLQLGWTTTKEEEQ--DEAALAA 118

Query: 432 LK-PPSSNVIFKCRTCQ 479
            K     + + +CR C+
Sbjct: 119 AKVKAKGSSVVRCRACK 135



 Score = 33.5 bits (73), Expect = 0.026
 Identities = 18/56 (32%), Positives = 33/56 (58%), Gaps = 2/56 (3%)
 Frame = +1

Query: 115 WADEVEIDQGVLPPPSEVVEN--GLKIVTEYKYDNDNKKVKIVRTYKIENVLFQRV 276
           WAD+ +   G+  P  +  +N  G K + E++ D++ KKVK+ R  + + V+ +RV
Sbjct: 9   WADDEDYGTGL--PSIQTFDNPDGTKTMIEFRIDDNGKKVKVTRVIR-KTVITERV 61


>SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large
           subunit|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1752

 Score = 31.1 bits (67), Expect = 0.14
 Identities = 17/44 (38%), Positives = 22/44 (50%)
 Frame = +3

Query: 384 TSKEESQRPDDGELDGLKPPSSNVIFKCRTCQETI*HCIVHSAH 515
           T  E  QRP  G L   +  + +  FKC+TC ET+  C  H  H
Sbjct: 42  TMDESGQRPRVGGLLDPRLGTIDRQFKCQTCGETMADCPGHFGH 85


>SPBC16E9.01c |php4|SPBP16F5.09c|CCAAT-binding factor complex
           subunit Php4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 295

 Score = 26.6 bits (56), Expect = 3.0
 Identities = 11/28 (39%), Positives = 16/28 (57%)
 Frame = +1

Query: 124 EVEIDQGVLPPPSEVVENGLKIVTEYKY 207
           EVE++  V+ P S   EN  + V +Y Y
Sbjct: 114 EVEVNSEVVKPDSATTENENRYVNQYNY 141


>SPCC553.10 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 349

 Score = 25.4 bits (53), Expect = 6.9
 Identities = 12/31 (38%), Positives = 20/31 (64%)
 Frame = -3

Query: 173 STTSEGGGSTPWSISTSSAQEA*NSSATGMS 81
           STTS    +TP + S+SS+  + +SS++  S
Sbjct: 131 STTSSSSSATPSTTSSSSSSSSSSSSSSSKS 161


>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1485

 Score = 25.0 bits (52), Expect = 9.1
 Identities = 20/73 (27%), Positives = 32/73 (43%), Gaps = 8/73 (10%)
 Frame = +3

Query: 219  QESENCQDIQN*--KRVVSKSIAKRKTWSKFGDSASD------KPGPNPATTNVAEDVFM 374
            +E ++ +D  N   K+   KS   RK  +  G S+S            P+TTN  +   +
Sbjct: 1215 EEEQSSRDFVNRTKKKPRGKSTGTRKPRAIAGSSSSTAVKKEASSESKPSTTNRKQQTLL 1274

Query: 375  QFITSKEESQRPD 413
            +F  SKE  +  D
Sbjct: 1275 EFAASKEPEKSSD 1287


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,379,847
Number of Sequences: 5004
Number of extensions: 43601
Number of successful extensions: 126
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 118
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 126
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 283719918
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)