Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--1077
         (637 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g06000.1 68418.m00665 eukaryotic translation initiation facto...    40   0.001
At3g11400.1 68416.m01390 eukaryotic translation initiation facto...    38   0.006
At1g52650.1 68414.m05945 F-box family protein contains F-box dom...    28   6.0  
At1g76760.1 68414.m08933 thioredoxin family protein similar to t...    27   7.9  
At1g67370.1 68414.m07669 meiotic asynaptic mutant 1 (ASY1) ident...    27   7.9  

>At5g06000.1 68418.m00665 eukaryotic translation initiation factor
           3G, putative / eIF3g, putative similar to eukaryotic
           translation initiation factor 3g [Arabidopsis thaliana]
           GI:12407751; contains InterPro entry IPR000504:
           RNA-binding region RNP-1 (RNA recognition motif) (RRM)
          Length = 276

 Score = 40.3 bits (90), Expect = 0.001
 Identities = 23/79 (29%), Positives = 40/79 (50%), Gaps = 3/79 (3%)
 Frame = +3

Query: 255 KRVVSKSIAKRKTWSKFGDSASDKPGPNPATTNVAEDVFMQFIT---SKEESQRPDDGEL 425
           KR ++K   +R++W+KFGD+A ++   +  T    ED+ ++ I    S  E        +
Sbjct: 34  KRALTKQAVERRSWNKFGDAAHEE-SSSYLTMRSTEDIILERIRAPGSNAEQSTVSGDSM 92

Query: 426 DGLKPPSSNVIFKCRTCQE 482
             L  P + V+  CR CQ+
Sbjct: 93  SQLGKPGA-VLMVCRLCQK 110



 Score = 34.7 bits (76), Expect = 0.052
 Identities = 13/28 (46%), Positives = 22/28 (78%)
 Frame = +1

Query: 172 ENGLKIVTEYKYDNDNKKVKIVRTYKIE 255
           +NG+K V EYK++ ++KKVKI  T +++
Sbjct: 6   QNGVKKVIEYKFNEEDKKVKITTTTRVQ 33


>At3g11400.1 68416.m01390 eukaryotic translation initiation factor
           3G / eIF3g nearly identical to eukaryotic translation
           initiation factor 3g [Arabidopsis thaliana] GI:12407751
          Length = 294

 Score = 37.9 bits (84), Expect = 0.006
 Identities = 22/62 (35%), Positives = 34/62 (54%), Gaps = 3/62 (4%)
 Frame = +1

Query: 100 EFQASWADEVEIDQGVLPPPSEVV---ENGLKIVTEYKYDNDNKKVKIVRTYKIENVLFQ 270
           +F+    DE E D   L PP +V+   ENGLK   EYK++++  KVKI    ++  +   
Sbjct: 11  KFRWGEMDEDE-DLDFLLPPKQVIGPDENGLKTTIEYKFNDEENKVKITTRTRVRKLASA 69

Query: 271 RV 276
           R+
Sbjct: 70  RL 71



 Score = 31.9 bits (69), Expect = 0.37
 Identities = 22/77 (28%), Positives = 40/77 (51%), Gaps = 4/77 (5%)
 Frame = +3

Query: 264 VSKSIAKRKTWSKFGDSASDKPGPNPATTNVAEDVFMQFI----TSKEESQRPDDGELDG 431
           ++K   +R+ W KFGD+A+++ G +  T    E++ ++      T  +ES+   DG L  
Sbjct: 71  LNKRAMERRNWPKFGDAANEEAGSH-LTMVSTEEILLERPRAPGTKADESKATGDG-LSQ 128

Query: 432 LKPPSSNVIFKCRTCQE 482
           L    + V+  CR C +
Sbjct: 129 LGKGGA-VLMVCRICHK 144


>At1g52650.1 68414.m05945 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 465

 Score = 27.9 bits (59), Expect = 6.0
 Identities = 12/31 (38%), Positives = 20/31 (64%)
 Frame = -1

Query: 187 FLVHFQPLQKVVEVHLGQSRPHQPRKPEILQ 95
           FL HF P  + +++H+ ++ P Q R PE+ Q
Sbjct: 404 FLEHF-PCLREIKIHMEENGPTQLRVPEVSQ 433


>At1g76760.1 68414.m08933 thioredoxin family protein similar to
           thioredoxin CH2, M-type, chloroplast precursor GB:P23400
           SP|P23400 [Chlamydomonas reinhardtii]; contains Pfam
           profile: PF00085 Thioredoxin
          Length = 172

 Score = 27.5 bits (58), Expect = 7.9
 Identities = 17/58 (29%), Positives = 29/58 (50%)
 Frame = +1

Query: 82  DMPVAEEFQASWADEVEIDQGVLPPPSEVVENGLKIVTEYKYDNDNKKVKIVRTYKIE 255
           D PV  ++ A+W    +    +L   SE +++ +++V   K D + K   I   YKIE
Sbjct: 81  DKPVLVDYYATWCGPCQFMVPILNEVSETLKDKIQVV---KIDTE-KYPSIANKYKIE 134


>At1g67370.1 68414.m07669 meiotic asynaptic mutant 1 (ASY1)
           identical to meiotic asynaptic mutant 1 [Arabidopsis
           thaliana] GI:7939627; contains Pfam profiles PF02301:
           DNA-binding HORMA domain, PF04433: SWIRM domain
          Length = 596

 Score = 27.5 bits (58), Expect = 7.9
 Identities = 14/40 (35%), Positives = 22/40 (55%)
 Frame = +3

Query: 309 DSASDKPGPNPATTNVAEDVFMQFITSKEESQRPDDGELD 428
           DS  D   P+ + + +++    QFI +  E Q  DDGE+D
Sbjct: 252 DSVHDDQ-PSDSDSEISQTQENQFIVAPVEKQDDDDGEVD 290


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,467,034
Number of Sequences: 28952
Number of extensions: 228964
Number of successful extensions: 668
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 648
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 666
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1305036432
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)