Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1072
(651 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 153 7e-38
At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 153 1e-37
At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 153 1e-37
At4g10710.1 68417.m01751 transcriptional regulator-related simil... 31 0.66
At1g29760.1 68414.m03639 expressed protein 31 0.66
At4g08180.3 68417.m01353 oxysterol-binding family protein simila... 29 2.7
At4g08180.2 68417.m01352 oxysterol-binding family protein simila... 29 2.7
At4g08180.1 68417.m01351 oxysterol-binding family protein simila... 29 2.7
At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, ... 29 2.7
At5g05030.1 68418.m00534 expressed protein contains similarity t... 28 4.7
At3g14075.1 68416.m01778 lipase class 3 family protein low simil... 28 6.2
>At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal
protein S4, Arabidopsis thaliana, PIR:T48480
Length = 262
Score = 153 bits (372), Expect = 7e-38
Identities = 77/133 (57%), Positives = 92/133 (69%), Gaps = 2/133 (1%)
Frame = +1
Query: 256 AGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHD 435
AGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+D
Sbjct: 84 AGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYD 143
Query: 436 GRTIRYPDPLIKVNDSIQLDIATTRLWTSSSLSPG-TCDDHGRRNLGRVGTSCPRET-SG 609
GRTIRYPDPLIK ND+I+LD+ ++ G G RN GRVG RE G
Sbjct: 144 GRTIRYPDPLIKPNDTIKLDLEANKIVEFIKFDVGNVVMVTGGRNRGRVGVIKNREKHKG 203
Query: 610 SFDIVHIKDSTGH 648
SF+ +HI+DSTGH
Sbjct: 204 SFETIHIQDSTGH 216
Score = 138 bits (335), Expect = 2e-33
Identities = 63/82 (76%), Positives = 71/82 (86%)
Frame = +2
Query: 8 MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 187
MARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT E
Sbjct: 1 MARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYRE 60
Query: 188 VLKIVKQRLIKVDGKVRTDPTY 253
V+ I+ QR I+VDGKVRTD TY
Sbjct: 61 VISILMQRHIQVDGKVRTDKTY 82
>At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B)
Length = 262
Score = 153 bits (371), Expect = 1e-37
Identities = 77/133 (57%), Positives = 92/133 (69%), Gaps = 2/133 (1%)
Frame = +1
Query: 256 AGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHD 435
AGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+D
Sbjct: 84 AGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYD 143
Query: 436 GRTIRYPDPLIKVNDSIQLDIATTRLWTSSSLSPG-TCDDHGRRNLGRVGTSCPRET-SG 609
GRTIRYPDPLIK ND+I+LD+ ++ G G RN GRVG RE G
Sbjct: 144 GRTIRYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRNRGRVGVIKNREKHKG 203
Query: 610 SFDIVHIKDSTGH 648
SF+ +HI+DSTGH
Sbjct: 204 SFETIHIQDSTGH 216
Score = 138 bits (335), Expect = 2e-33
Identities = 63/82 (76%), Positives = 71/82 (86%)
Frame = +2
Query: 8 MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 187
MARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT E
Sbjct: 1 MARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYRE 60
Query: 188 VLKIVKQRLIKVDGKVRTDPTY 253
V+ I+ QR I+VDGKVRTD TY
Sbjct: 61 VISILMQRHIQVDGKVRTDKTY 82
>At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains
ribosomal protein S4 signature from residues 8 to 22
Length = 261
Score = 153 bits (371), Expect = 1e-37
Identities = 77/133 (57%), Positives = 92/133 (69%), Gaps = 2/133 (1%)
Frame = +1
Query: 256 AGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHD 435
AGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+D
Sbjct: 84 AGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYD 143
Query: 436 GRTIRYPDPLIKVNDSIQLDIATTRLWTSSSLSPG-TCDDHGRRNLGRVGTSCPRET-SG 609
GRTIRYPDPLIK ND+I+LD+ ++ G G RN GRVG RE G
Sbjct: 144 GRTIRYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRNRGRVGVIKNREKHKG 203
Query: 610 SFDIVHIKDSTGH 648
SF+ +HI+DSTGH
Sbjct: 204 SFETIHIQDSTGH 216
Score = 138 bits (335), Expect = 2e-33
Identities = 63/82 (76%), Positives = 71/82 (86%)
Frame = +2
Query: 8 MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 187
MARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT E
Sbjct: 1 MARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYRE 60
Query: 188 VLKIVKQRLIKVDGKVRTDPTY 253
V+ I+ QR I+VDGKVRTD TY
Sbjct: 61 VISILMQRHIQVDGKVRTDKTY 82
>At4g10710.1 68417.m01751 transcriptional regulator-related similar
to chromatin-specific transcription elongation factor
FACT 140 kDa subunit (GI:5499741) [Homo sapiens]
Length = 1074
Score = 31.1 bits (67), Expect = 0.66
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -1
Query: 132 KHSRNLWGPVDGLGAYTPPSLSNIHAL 52
KHS +LWG D L TPP+ ++ L
Sbjct: 44 KHSADLWGSADALAIATPPASDDLRYL 70
>At1g29760.1 68414.m03639 expressed protein
Length = 526
Score = 31.1 bits (67), Expect = 0.66
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = -1
Query: 243 SVLTFPSTFMRRCFTIFRTSFPVKAYFRRFL 151
S+LTFP +R CF F F + RRFL
Sbjct: 195 SLLTFPPWLLRNCFLFFFDPFSTIRFGRRFL 225
>At4g08180.3 68417.m01353 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 29.1 bits (62), Expect = 2.7
Identities = 13/34 (38%), Positives = 21/34 (61%)
Frame = +2
Query: 155 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTYL 256
N ++A+T NE+ +K+RL D ++R D YL
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYL 736
>At4g08180.2 68417.m01352 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 29.1 bits (62), Expect = 2.7
Identities = 13/34 (38%), Positives = 21/34 (61%)
Frame = +2
Query: 155 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTYL 256
N ++A+T NE+ +K+RL D ++R D YL
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYL 736
>At4g08180.1 68417.m01351 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 814
Score = 29.1 bits (62), Expect = 2.7
Identities = 13/34 (38%), Positives = 21/34 (61%)
Frame = +2
Query: 155 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTYL 256
N ++A+T NE+ +K+RL D ++R D YL
Sbjct: 704 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYL 737
>At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase,
putative / steroid 5-alpha-reductase, putative identical
to gi:1280611; contains a steroid 5-alpha reductase,
C-terminal domain
Length = 262
Score = 29.1 bits (62), Expect = 2.7
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = -1
Query: 222 TFMRRCFTIFRTSFPVKAYFRRFLRKITRGKHSRNLWGP 106
TF R C + P A +FL+ GKH+R WGP
Sbjct: 8 TFFRYCLLTLIFAGPPTAVLLKFLQA-PYGKHNRTGWGP 45
>At5g05030.1 68418.m00534 expressed protein contains similarity to
carboxyl-terminal proteinase contains Pfam profile
PF03080: Arabidopsis proteins of unknown function
Length = 363
Score = 28.3 bits (60), Expect = 4.7
Identities = 15/57 (26%), Positives = 24/57 (42%)
Frame = +1
Query: 403 PKNVPYLVTHDGRTIRYPDPLIKVNDSIQLDIATTRLWTSSSLSPGTCDDHGRRNLG 573
PKN + I P+ + + + + +A + WT L+P T D HG G
Sbjct: 67 PKNQVKGESERKNIIECPNGTVPIIRNTKKYVANAQYWTEKHLNPLTIDSHGTHIAG 123
>At3g14075.1 68416.m01778 lipase class 3 family protein low
similarity to calmodulin-binding heat-shock protein
CaMBP [Nicotiana tabacum] GI:1087073; contains Pfam
profile PF01764: Lipase, PF03893: Lipase 3 N-terminal
region
Length = 642
Score = 27.9 bits (59), Expect = 6.2
Identities = 11/31 (35%), Positives = 21/31 (67%), Gaps = 1/31 (3%)
Frame = -2
Query: 551 SSQVPGLKLDEV-HNLVVAMSNWMESLTLIS 462
S+ P L++D V H L+ A + W+E+++ +S
Sbjct: 39 SASRPSLRIDRVSHRLIQAPATWLETISTLS 69
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,842,195
Number of Sequences: 28952
Number of extensions: 339517
Number of successful extensions: 955
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 913
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 949
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1354097952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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