BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--1068 (646 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC694.04c |||conserved eukaryotic protein|Schizosaccharomyces ... 83 3e-17 SPBC21D10.12 |hob1||BAR adaptor protein Hob1|Schizosaccharomyces... 29 0.76 SPBP19A11.04c |mor2|cps12|morphogenesis protein Mor2|Schizosacch... 27 3.1 SPAC6G10.02c |tea3||cell end marker Tea3|Schizosaccharomyces pom... 26 4.0 SPBC1703.08c |||5-formyltetrahydrofolate cyclo-ligase|Schizosacc... 26 4.0 SPBC6B1.12c |sus1||SAGA complex subunit Sus1 |Schizosaccharomyce... 25 7.1 SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 25 9.3 SPCC364.05 |vps3||GTPase regulator Vps3 |Schizosaccharomyces pom... 25 9.3 >SPAC694.04c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 324 Score = 83.4 bits (197), Expect = 3e-17 Identities = 37/73 (50%), Positives = 50/73 (68%) Frame = +2 Query: 260 MKIGTHDGVFHCDEVLACFMLKNLPQYKDAEIIRTRDLNKLNDCDIVVDVGSVFDHEKKR 439 +KI TH G FH DE LA +ML+ L ++ A+I+R+RD L+ CDI+VDVG +D K Sbjct: 5 VKIATHSGTFHADEALAVYMLRRLDRFSGAQIVRSRDPQVLDSCDIIVDVGGKYD-GIKY 63 Query: 440 YDHHQAGFNETLS 478 +DHHQ FN+T S Sbjct: 64 FDHHQREFNDTFS 76 Score = 44.0 bits (99), Expect = 2e-05 Identities = 17/44 (38%), Positives = 32/44 (72%) Frame = +1 Query: 514 KLSSAGLVYAYYGEDIIQQLKEESTSLTNEDLKLIYKKVYESFI 645 +LSSAGL+Y ++G ++I + + + +DL+ +Y+KVY+SF+ Sbjct: 82 RLSSAGLIYKHFGREVIHAVLPQ-LKINEQDLETLYEKVYQSFV 124 >SPBC21D10.12 |hob1||BAR adaptor protein Hob1|Schizosaccharomyces pombe|chr 2|||Manual Length = 466 Score = 28.7 bits (61), Expect = 0.76 Identities = 22/68 (32%), Positives = 31/68 (45%), Gaps = 4/68 (5%) Frame = +2 Query: 323 KNLPQYKDAEIIRTRDLNKLNDC-DIVVDVGSVF---DHEKKRYDHHQAGFNETLSTLRP 490 KNL D +I TR +N + IV DV + DH++ YD H++ F + Sbjct: 115 KNLASEMD--VINTRIVNPTGELLKIVKDVDKLLLKRDHKQLDYDRHRSSFKKLQEKKDK 172 Query: 491 ELGDSYKL 514 L D KL Sbjct: 173 SLKDEKKL 180 >SPBP19A11.04c |mor2|cps12|morphogenesis protein Mor2|Schizosaccharomyces pombe|chr 2|||Manual Length = 2196 Score = 26.6 bits (56), Expect = 3.1 Identities = 11/23 (47%), Positives = 14/23 (60%) Frame = -2 Query: 429 SWSKTLPTSTTISQSFSLFRSLV 361 SW+ T P+ SF LFRSL+ Sbjct: 1578 SWATTCPSRRLACNSFQLFRSLL 1600 >SPAC6G10.02c |tea3||cell end marker Tea3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1125 Score = 26.2 bits (55), Expect = 4.0 Identities = 19/68 (27%), Positives = 34/68 (50%) Frame = -3 Query: 452 GDDHNASFHGQKHFLHQQLYHSHSAYLDLLCG*SQHLCIEEDSLA*SKLELHRSGILRRE 273 G A+ + QK+ QQL + + +LL ++ LC++ D L S E S IL + Sbjct: 664 GTLEEATSYYQKNTELQQLLKQNESASELLKSRNEKLCVDYDKLR-SVFEEDSSKILSLQ 722 Query: 272 SQSSSLNT 249 ++ +L + Sbjct: 723 KENENLQS 730 >SPBC1703.08c |||5-formyltetrahydrofolate cyclo-ligase|Schizosaccharomyces pombe|chr 2|||Manual Length = 204 Score = 26.2 bits (55), Expect = 4.0 Identities = 11/53 (20%), Positives = 26/53 (49%), Gaps = 1/53 (1%) Frame = +2 Query: 386 DCDIVVDVGSVFDHEKKRYDHHQAGFNETLSTLRP-ELGDSYKLNLVQQALCM 541 DC++++ G FD + R H + ++ +S + L + N+ + +C+ Sbjct: 120 DCELIIVPGVAFDEKLSRLGHGKGYYDNYISKYQSWALQKESRANMFKVGICL 172 >SPBC6B1.12c |sus1||SAGA complex subunit Sus1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 108 Score = 25.4 bits (53), Expect = 7.1 Identities = 9/24 (37%), Positives = 17/24 (70%) Frame = +1 Query: 568 QLKEESTSLTNEDLKLIYKKVYES 639 QL++ + + N D K+ ++K+YES Sbjct: 44 QLRDYTRGIVNSDSKIDFQKLYES 67 >SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 646 Score = 25.0 bits (52), Expect = 9.3 Identities = 10/34 (29%), Positives = 19/34 (55%) Frame = +1 Query: 472 LEYLEA*TRRQL*IKLSSAGLVYAYYGEDIIQQL 573 L+ LE RR + +K + ++Y Y+G+ + L Sbjct: 95 LDKLEVERRRGITVKAQTCSMIYYYHGQSYLLNL 128 >SPCC364.05 |vps3||GTPase regulator Vps3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 910 Score = 25.0 bits (52), Expect = 9.3 Identities = 11/28 (39%), Positives = 18/28 (64%) Frame = +2 Query: 461 FNETLSTLRPELGDSYKLNLVQQALCML 544 F+ TLS + ++ D + LNL+ LC+L Sbjct: 839 FSYTLSYVCEQIPDHWNLNLLADFLCIL 866 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,461,737 Number of Sequences: 5004 Number of extensions: 48744 Number of successful extensions: 132 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 127 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 130 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 289756512 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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