BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--1048 (640 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g17240.1 68417.m02592 expressed protein 30 1.5 At4g38990.1 68417.m05524 glycosyl hydrolase family 9 protein end... 29 2.6 At3g44940.1 68416.m04842 expressed protein 29 2.6 At1g33880.1 68414.m04200 avirulence-responsive family protein / ... 29 3.4 At4g31160.1 68417.m04423 transducin family protein / WD-40 repea... 27 7.9 >At4g17240.1 68417.m02592 expressed protein Length = 200 Score = 29.9 bits (64), Expect = 1.5 Identities = 14/33 (42%), Positives = 20/33 (60%) Frame = -3 Query: 341 KIIVLLPDNKRSKIEQMFELQHLKTKFPSLKKR 243 K VL PD+ S+I QM EL + P+L+K+ Sbjct: 41 KSAVLFPDSMNSQINQMQELNQAREIIPNLQKQ 73 >At4g38990.1 68417.m05524 glycosyl hydrolase family 9 protein endo-1,4-beta-glucanase precursor - Fragariax ananassa, PID:g3549291 A short intron was annotated between exons 4 and 5 to circumvent a frameshift. The frameshift may be artificial due to a sequencing error, or alternatively is genuine suggesting a truncated protein or pseudogene. Length = 495 Score = 29.1 bits (62), Expect = 2.6 Identities = 11/37 (29%), Positives = 21/37 (56%) Frame = +3 Query: 162 CRFLALPLILVPSWVRHEISQMTEELLALFERGKLGF 272 C F+ + LIL+P+ + H+ S + + FE + G+ Sbjct: 6 CSFIVILLILLPTTISHDYSDALTKSILFFEGQRSGY 42 >At3g44940.1 68416.m04842 expressed protein Length = 250 Score = 29.1 bits (62), Expect = 2.6 Identities = 19/53 (35%), Positives = 31/53 (58%), Gaps = 5/53 (9%) Frame = -3 Query: 311 RSKIEQMFELQHLKTKFPSLKKRKELL---SHL--GNLMTNPRRHQNQRQSEK 168 R + EQ+ L+ + TK +LK+R E L +HL NL+ ++ QNQ Q ++ Sbjct: 45 RKRDEQLIHLEDVLTK--TLKERDEALEKYNHLLLNNLLLQQKQQQNQNQKQE 95 >At1g33880.1 68414.m04200 avirulence-responsive family protein / avirulence induced gene (AIG1) family protein similar to AIG1 protein SP:P54120 [Arabidopsis thaliana]; contains Pfam PF04548: AIG1 family; Length = 234 Score = 28.7 bits (61), Expect = 3.4 Identities = 19/61 (31%), Positives = 32/61 (52%), Gaps = 2/61 (3%) Frame = +3 Query: 204 VRHEISQMTEELLALFERGKLGFQMLKFKH--LFNLTAFIIREKDDYFPICCPAALNAEM 377 +++ I+Q E L +R G ++L++ L + F +E +DYFP CCP L + Sbjct: 113 MKNRITQEEEYALNTLQR-IFGSKILEYLIFLLIDGEKFEAKEFEDYFPECCPEFLMRVL 171 Query: 378 R 380 R Sbjct: 172 R 172 >At4g31160.1 68417.m04423 transducin family protein / WD-40 repeat family protein contains 2 WD-40 repeats (PF00400); KIAA0800 protein, Homo sapiens GI:3882321 EMBL:AB018343 Length = 1846 Score = 27.5 bits (58), Expect = 7.9 Identities = 12/35 (34%), Positives = 19/35 (54%) Frame = -3 Query: 419 LTKSRHDVFLEARSHFGVKRCWTTDGKIIVLLPDN 315 L +S+ +FLE HF R GK+ +L+ +N Sbjct: 79 LLESQESLFLEENGHFSNARGSHNSGKLCILIREN 113 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,876,660 Number of Sequences: 28952 Number of extensions: 278848 Number of successful extensions: 752 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 739 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 752 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1314848736 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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