Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1017
(388 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g34555.1 68417.m04910 40S ribosomal protein S25, putative 71 4e-13
At4g39200.1 68417.m05550 40S ribosomal protein S25 (RPS25E) ribo... 69 1e-12
At2g21580.1 68415.m02567 40S ribosomal protein S25 (RPS25B) 69 1e-12
At2g16360.1 68415.m01872 40S ribosomal protein S25 (RPS25A) 68 3e-12
At5g64950.1 68418.m08170 mitochondrial transcription termination... 31 0.36
At1g14500.1 68414.m01719 ankyrin repeat family protein contains ... 29 1.4
At2g17250.1 68415.m01992 expressed protein weak similarity to Ri... 28 2.5
At5g44415.1 68418.m05439 hypothetical protein contains Pfam prof... 27 4.4
At4g09370.1 68417.m01546 hypothetical protein contains Pfam prof... 27 4.4
At2g23710.1 68415.m02831 hypothetical protein contains Pfam prof... 27 4.4
At5g37390.1 68418.m04498 hypothetical protein contains Pfam prof... 27 5.8
At2g23170.1 68415.m02768 auxin-responsive GH3 family protein sim... 27 5.8
At1g24706.1 68414.m03104 expressed protein 27 5.8
At5g54770.1 68418.m06822 thiazole biosynthetic enzyme, chloropla... 26 7.6
At1g48180.1 68414.m05378 expressed protein ; expression supporte... 26 7.6
>At4g34555.1 68417.m04910 40S ribosomal protein S25, putative
Length = 108
Score = 70.5 bits (165), Expect = 4e-13
Identities = 30/53 (56%), Positives = 43/53 (81%)
Frame = +1
Query: 109 LNNQVLFDKPTYEKLYKEVPQYKLITPAVVSERLKVRGSLARRALIELRKKAS 267
+NN VLFD+ TY+KL E P++KLITP+++S+RL++ GSLARRA+ EL K +
Sbjct: 37 VNNMVLFDQATYDKLLSEAPKFKLITPSILSDRLRINGSLARRAIRELMAKGT 89
Score = 29.1 bits (62), Expect = 1.1
Identities = 13/22 (59%), Positives = 14/22 (63%)
Frame = +3
Query: 258 KGLIKQVVQHHGQVIYTRATKG 323
KG I+ V H Q IYTRAT G
Sbjct: 87 KGTIRMVSAHSSQQIYTRATHG 108
>At4g39200.1 68417.m05550 40S ribosomal protein S25 (RPS25E)
ribosomal protein S25, Lycopersicon esculentum,
PIR2:S40089
Length = 108
Score = 68.9 bits (161), Expect = 1e-12
Identities = 29/51 (56%), Positives = 42/51 (82%)
Frame = +1
Query: 109 LNNQVLFDKPTYEKLYKEVPQYKLITPAVVSERLKVRGSLARRALIELRKK 261
+NN VLFD+ TY+KL E P++KLITP+++S+R+++ GSLARRA+ EL K
Sbjct: 37 VNNMVLFDQATYDKLLTEAPKFKLITPSILSDRMRINGSLARRAIRELMAK 87
Score = 28.7 bits (61), Expect = 1.4
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = +3
Query: 258 KGLIKQVVQHHGQVIYTRAT 317
KG+I+ V H Q IYTRAT
Sbjct: 87 KGVIRMVAAHSSQQIYTRAT 106
>At2g21580.1 68415.m02567 40S ribosomal protein S25 (RPS25B)
Length = 108
Score = 68.5 bits (160), Expect = 1e-12
Identities = 29/51 (56%), Positives = 42/51 (82%)
Frame = +1
Query: 109 LNNQVLFDKPTYEKLYKEVPQYKLITPAVVSERLKVRGSLARRALIELRKK 261
+NN VLFD+ TY+KL E P++KLITP+++S+R+++ GSLARRA+ EL K
Sbjct: 37 VNNMVLFDQGTYDKLLTEAPKFKLITPSILSDRMRINGSLARRAIRELMAK 87
Score = 29.1 bits (62), Expect = 1.1
Identities = 13/20 (65%), Positives = 14/20 (70%)
Frame = +3
Query: 258 KGLIKQVVQHHGQVIYTRAT 317
KGLI+ V H Q IYTRAT
Sbjct: 87 KGLIRMVSAHSSQQIYTRAT 106
>At2g16360.1 68415.m01872 40S ribosomal protein S25 (RPS25A)
Length = 125
Score = 67.7 bits (158), Expect = 3e-12
Identities = 28/53 (52%), Positives = 43/53 (81%)
Frame = +1
Query: 109 LNNQVLFDKPTYEKLYKEVPQYKLITPAVVSERLKVRGSLARRALIELRKKAS 267
+NN VLFD+ TY+KL E P++KLITP+++S+RL++ GSLAR+A+ +L K +
Sbjct: 53 VNNMVLFDQATYDKLMSEAPKFKLITPSILSDRLRINGSLARKAIRDLMVKGT 105
>At5g64950.1 68418.m08170 mitochondrial transcription termination
factor-related / mTERF-related contains Pfam profile
PF02536: mTERF
Length = 391
Score = 30.7 bits (66), Expect = 0.36
Identities = 17/49 (34%), Positives = 26/49 (53%)
Frame = -3
Query: 344 SLCDWIIALGRTRVDHLPMVLDYLFDEAFFLSSMSALLAREPRTFNLSD 198
S C W++ + LP + YL S +++LL R+PR FNLS+
Sbjct: 166 SRCGWLLLSRDPNLFLLPNI-SYLETCGIVGSQLASLLRRQPRIFNLSE 213
>At1g14500.1 68414.m01719 ankyrin repeat family protein contains
Pfam domain, PF00023: Ankyrin repeat
Length = 436
Score = 28.7 bits (61), Expect = 1.4
Identities = 12/49 (24%), Positives = 24/49 (48%), Gaps = 2/49 (4%)
Frame = -3
Query: 173 YCGTSLYSFSYVGLSN--NTWLFNLSRTFPLDHFFFLALPPPDPSFFFC 33
+C LY+F + + + TW F + + + + +A+ P+P F C
Sbjct: 342 FCCALLYTFCLLPIGSLFTTWFFWIGASLGVSYALAMAIISPNPLLFLC 390
>At2g17250.1 68415.m01992 expressed protein weak similarity to
Ribosome biogenesis protein MAK21 (Swiss-Prot:Q12176)
[Saccharomyces cerevisiae]
Length = 577
Score = 27.9 bits (59), Expect = 2.5
Identities = 13/47 (27%), Positives = 29/47 (61%)
Frame = +1
Query: 112 NNQVLFDKPTYEKLYKEVPQYKLITPAVVSERLKVRGSLARRALIEL 252
+++ + +KPT +K E L++PA +S+R+K++ + A + + L
Sbjct: 245 SDESISEKPTDKKKKTEKGDSTLLSPATISKRMKLKFTKAWISFLRL 291
>At5g44415.1 68418.m05439 hypothetical protein contains Pfam profile
PF03384: Drosophila protein of unknown function, DUF287
Length = 611
Score = 27.1 bits (57), Expect = 4.4
Identities = 12/36 (33%), Positives = 17/36 (47%)
Frame = +2
Query: 215 SEVPWREEHSSNLGKRPHQTGSPTPWAGDLHACDQG 322
+E+P R+E + L + G P GD A D G
Sbjct: 31 AEIPQRDEEGAELLTTEEEAGEIQPTTGDCDAADLG 66
>At4g09370.1 68417.m01546 hypothetical protein contains Pfam profile
PF03384: Drosophila protein of unknown function, DUF287
Length = 666
Score = 27.1 bits (57), Expect = 4.4
Identities = 12/36 (33%), Positives = 17/36 (47%)
Frame = +2
Query: 215 SEVPWREEHSSNLGKRPHQTGSPTPWAGDLHACDQG 322
+E+P R+E + L + G P GD A D G
Sbjct: 31 AEIPRRDEEGAELFTTEEEAGEIQPTTGDCDAADLG 66
>At2g23710.1 68415.m02831 hypothetical protein contains Pfam profile
PF03384: Drosophila protein of unknown function, DUF287;
this may be a pseudogene; the protein appears slightly
truncated on the 3' end due to intervening stop codon.
Length = 592
Score = 27.1 bits (57), Expect = 4.4
Identities = 12/36 (33%), Positives = 17/36 (47%)
Frame = +2
Query: 215 SEVPWREEHSSNLGKRPHQTGSPTPWAGDLHACDQG 322
+E+P R+E + L + G P GD A D G
Sbjct: 31 AEIPQRDEEGAELLTTEEEAGEIQPTTGDCDAADLG 66
>At5g37390.1 68418.m04498 hypothetical protein contains Pfam profile
PF03384: Drosophila protein of unknown function, DUF287
Length = 628
Score = 26.6 bits (56), Expect = 5.8
Identities = 14/45 (31%), Positives = 21/45 (46%)
Frame = +2
Query: 215 SEVPWREEHSSNLGKRPHQTGSPTPWAGDLHACDQGR*SSRINYD 349
+E+P R+E L + G P GD A D G S+ + +D
Sbjct: 31 AEIPQRDEEGVELLTTEEEAGEIQPTTGDCDAADLGN-SNAVAWD 74
>At2g23170.1 68415.m02768 auxin-responsive GH3 family protein
similar to auxin-responsive GH3 product [Glycine max]
GI:18591; contains Pfam profile PF03321: GH3
auxin-responsive promoter
Length = 595
Score = 26.6 bits (56), Expect = 5.8
Identities = 11/31 (35%), Positives = 19/31 (61%)
Frame = -2
Query: 147 LICGFIKQHLVVQLVTNFSFGPLLLLGFAAT 55
++CG + +H V++L F+ G L +GF T
Sbjct: 213 MLCGLLMRHEVLRLGAVFASGLLRAIGFLQT 243
>At1g24706.1 68414.m03104 expressed protein
Length = 1781
Score = 26.6 bits (56), Expect = 5.8
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = -3
Query: 110 NLSRTFPLDHFFFLALPPPDP 48
+L ++ P DHF LPPP P
Sbjct: 1562 SLEKSHPDDHFHSQGLPPPPP 1582
>At5g54770.1 68418.m06822 thiazole biosynthetic enzyme, chloroplast
(ARA6) (THI1) (THI4) identical to SP|Q38814 Thiazole
biosynthetic enzyme, chloroplast precursor (ARA6)
{Arabidopsis thaliana}
Length = 349
Score = 26.2 bits (55), Expect = 7.6
Identities = 14/33 (42%), Positives = 22/33 (66%), Gaps = 3/33 (9%)
Frame = -3
Query: 275 LFDEAFFLSSMSAL---LAREPRTFNLSDTTAG 186
LFD +F S++SA + +PR+F++ TTAG
Sbjct: 18 LFDSSFHGSAISAAPISIGLKPRSFSVRATTAG 50
>At1g48180.1 68414.m05378 expressed protein ; expression supported
by MPSS
Length = 239
Score = 26.2 bits (55), Expect = 7.6
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = -3
Query: 206 LSDTTAGVISLYCGTSLYSFSYVGL 132
L + + +YCGTS SYVGL
Sbjct: 149 LQQDASAITGIYCGTSGEPASYVGL 173
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,274,358
Number of Sequences: 28952
Number of extensions: 168900
Number of successful extensions: 546
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 534
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 546
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 547638520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -