Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--1016
         (394 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein |Schizo...    28   0.45 
SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase...    28   0.59 
SPBP4H10.10 |||rhomboid family protease|Schizosaccharomyces pomb...    28   0.59 
SPAC24H6.01c ||SPAPB21F2.01|membrane bound O-acyltransferase, MB...    28   0.59 
SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3 |Schizosacchar...    27   0.79 
SPAPYUK71.03c |||C2 domain protein|Schizosaccharomyces pombe|chr...    27   1.0  
SPAC23C4.02 |crn1||actin binding protein, coronin Crn1|Schizosac...    27   1.0  
SPAC1527.03 |||RNA-binding protein|Schizosaccharomyces pombe|chr...    26   1.8  
SPBC17F3.02 |nak1|orb3, mor4|PAK-related kinase Nak1|Schizosacch...    25   3.2  
SPAC1805.04 |nup132|Nup133b, Nup133b|nucleoporin Nup132|Schizosa...    25   4.2  
SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S...    25   5.5  
SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo...    25   5.5  
SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces ...    25   5.5  
SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces p...    24   7.3  
SPCC1840.08c |||protein disulfide isomerase |Schizosaccharomyces...    24   9.7  
SPAC17G6.12 |cul1|pcu1|cullin 1|Schizosaccharomyces pombe|chr 1|...    24   9.7  

>SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 391

 Score = 28.3 bits (60), Expect = 0.45
 Identities = 17/40 (42%), Positives = 18/40 (45%)
 Frame = +1

Query: 160 VEEKSTVQVNTMEDDSVDLSSVETITLNKVNMPSIGFSLE 279
           VE KSTVQ N    DS  LS      +NK N      S E
Sbjct: 107 VESKSTVQRNASGADSTSLSHFTANEINKGNCKKTALSNE 146


>SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase
            Gpt1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1448

 Score = 27.9 bits (59), Expect = 0.59
 Identities = 22/84 (26%), Positives = 35/84 (41%)
 Frame = +1

Query: 19   LDLELRGVINIERSSASMLGTKVEIKLKKGEPGSWSKLDFPRTEPKKVEEKSTVQVNTME 198
            + L+L  + N   +   +L      +LK   PG W+         +  E  S  + N+ +
Sbjct: 1040 MQLKLGNLTNSHVTDTIVLSNLGYFQLK-ANPGVWTLEPMDGRSSQFYEILSLNKKNSYK 1098

Query: 199  DDSVDLSSVETITLNKVNMPSIGF 270
            D  V + S E +TLN V     GF
Sbjct: 1099 DPQVIVDSFEGVTLNPVMRRKPGF 1122


>SPBP4H10.10 |||rhomboid family protease|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 392

 Score = 27.9 bits (59), Expect = 0.59
 Identities = 11/24 (45%), Positives = 17/24 (70%)
 Frame = -2

Query: 105 LFQFYLHFSTQHRSRTSFDIDNPS 34
           +F+ Y+ FS  HRS +S D+ +PS
Sbjct: 37  IFRPYVIFSRSHRSLSSVDVSSPS 60


>SPAC24H6.01c ||SPAPB21F2.01|membrane bound O-acyltransferase, MBOAT
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 588

 Score = 27.9 bits (59), Expect = 0.59
 Identities = 12/33 (36%), Positives = 21/33 (63%)
 Frame = -3

Query: 251 LTLFNVIVSTELRSTESSSIVLTWTVDFSSTFF 153
           + L N ++S  L+++  + + LTWT+D S  FF
Sbjct: 177 IALINYLISKSLKNSIFNPL-LTWTLDISVVFF 208


>SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1131

 Score = 27.5 bits (58), Expect = 0.79
 Identities = 23/88 (26%), Positives = 37/88 (42%), Gaps = 2/88 (2%)
 Frame = -3

Query: 320 ISKAFISKMSQNCISREKPILGMLT--LFNVIVSTELRSTESSSIVLTWTVDFSSTFFGS 147
           +S    S M  + +S    +   L   + N  ++T      S    L  +   SSTF  S
Sbjct: 371 VSSISTSSMDSSAVSSYSVVQSSLASIISNAYIATSKSGLNSGVSTLLASPTSSSTFVTS 430

Query: 146 VLGKSSLDHEPGSPFFNFISTLVPNIEA 63
           +L +SS+D   GS   +  S  VP + +
Sbjct: 431 LLRRSSID---GSASSSSASLAVPTVSS 455


>SPAPYUK71.03c |||C2 domain protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1225

 Score = 27.1 bits (57), Expect = 1.0
 Identities = 14/33 (42%), Positives = 19/33 (57%)
 Frame = -3

Query: 146 VLGKSSLDHEPGSPFFNFISTLVPNIEAELLSI 48
           V+GKS + H  GSP FN     V N  +E L++
Sbjct: 468 VIGKSKVAHNTGSPVFNETFYSVLNSFSENLNL 500


>SPAC23C4.02 |crn1||actin binding protein, coronin
           Crn1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 601

 Score = 27.1 bits (57), Expect = 1.0
 Identities = 16/36 (44%), Positives = 19/36 (52%)
 Frame = +1

Query: 139 PRTEPKKVEEKSTVQVNTMEDDSVDLSSVETITLNK 246
           P  E KKVEEK    V+    D  D+S+V    LNK
Sbjct: 530 PLAESKKVEEKEPFYVS---KDKKDISAVNLADLNK 562


>SPAC1527.03 |||RNA-binding protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 475

 Score = 26.2 bits (55), Expect = 1.8
 Identities = 17/63 (26%), Positives = 30/63 (47%)
 Frame = +1

Query: 73  LGTKVEIKLKKGEPGSWSKLDFPRTEPKKVEEKSTVQVNTMEDDSVDLSSVETITLNKVN 252
           L + + IK+++ E  +WS    P     K E     Q+N+    S   SS+  +T++   
Sbjct: 395 LQSPMSIKVRRKE--TWSPWILPSESRLKFEMAKYPQINSSSSMSPLASSISNLTISPPF 452

Query: 253 MPS 261
           +PS
Sbjct: 453 IPS 455


>SPBC17F3.02 |nak1|orb3, mor4|PAK-related kinase
           Nak1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 652

 Score = 25.4 bits (53), Expect = 3.2
 Identities = 14/39 (35%), Positives = 20/39 (51%)
 Frame = -3

Query: 224 TELRSTESSSIVLTWTVDFSSTFFGSVLGKSSLDHEPGS 108
           + + S   +S   T     S+T  G+V+ KSS  HEP S
Sbjct: 331 SNVSSITGTSTSTTTAATSSTTVTGTVIPKSSTVHEPPS 369


>SPAC1805.04 |nup132|Nup133b, Nup133b|nucleoporin
           Nup132|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1162

 Score = 25.0 bits (52), Expect = 4.2
 Identities = 11/39 (28%), Positives = 22/39 (56%)
 Frame = +2

Query: 164 KKSQLSRSIRWKTIQSTSVQWKRLR*IRLTCLVLVFLSK 280
           K+  + +S+   T  + S+ W R +  +++CL+  F SK
Sbjct: 20  KRISVEKSLLDSTAYNNSLDWLRSKNFKVSCLLKHFNSK 58


>SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase
            Mok11|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 2397

 Score = 24.6 bits (51), Expect = 5.5
 Identities = 11/29 (37%), Positives = 16/29 (55%)
 Frame = -3

Query: 290  QNCISREKPILGMLTLFNVIVSTELRSTE 204
            QN   R+  +LGM  LFN   S +++  E
Sbjct: 1107 QNLHFRQHDVLGMKNLFNFSCSNKIKGEE 1135


>SPAC56F8.03 |||translation initiation factor IF2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1079

 Score = 24.6 bits (51), Expect = 5.5
 Identities = 15/46 (32%), Positives = 22/46 (47%), Gaps = 4/46 (8%)
 Frame = +1

Query: 88  EIKLKKGEPGSWSKLDFPRTEPKKVEE----KSTVQVNTMEDDSVD 213
           + K KKG+ G  SK      EP+++E     K    V  ++DD  D
Sbjct: 101 DYKPKKGKKGKKSKKVEEDDEPQEIESPQGPKELTAVTELDDDEFD 146


>SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1877

 Score = 24.6 bits (51), Expect = 5.5
 Identities = 15/53 (28%), Positives = 26/53 (49%)
 Frame = -3

Query: 254  MLTLFNVIVSTELRSTESSSIVLTWTVDFSSTFFGSVLGKSSLDHEPGSPFFN 96
            ++  F   V  ++ S E +  VLT  + F    FG  + ++ L+   GS F+N
Sbjct: 1163 LIVHFMKFVLKKITSMEVNLNVLTRELHFKFVSFGLRIAENLLNSPLGSRFYN 1215


>SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1323

 Score = 24.2 bits (50), Expect = 7.3
 Identities = 14/41 (34%), Positives = 22/41 (53%)
 Frame = +1

Query: 67   SMLGTKVEIKLKKGEPGSWSKLDFPRTEPKKVEEKSTVQVN 189
            +ML   ++I   K + G+ S       +PK+V EK  VQ+N
Sbjct: 1082 AMLACVLDIPNPKKQSGNTSADQVLFNQPKQVTEKLGVQLN 1122


>SPCC1840.08c |||protein disulfide isomerase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 561

 Score = 23.8 bits (49), Expect = 9.7
 Identities = 10/58 (17%), Positives = 26/58 (44%)
 Frame = +1

Query: 160 VEEKSTVQVNTMEDDSVDLSSVETITLNKVNMPSIGFSLEMQFCDILLMNALDILTYY 333
           ++ K   + +   D +  +  +     NK+   S+  +  ++ C+ + +N+   L YY
Sbjct: 125 IDSKKKPRQSFYTDSATSIVQLSKKYRNKIKFKSVDCASSLEKCEEIGINSFPSLVYY 182


>SPAC17G6.12 |cul1|pcu1|cullin 1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 767

 Score = 23.8 bits (49), Expect = 9.7
 Identities = 10/25 (40%), Positives = 15/25 (60%)
 Frame = -1

Query: 340 LNNNMLIYLKHSLARCHKIAFREKN 266
           L NN+++YL+  LAR  K    + N
Sbjct: 82  LYNNLVLYLEEYLARLRKECISQTN 106


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,528,998
Number of Sequences: 5004
Number of extensions: 30342
Number of successful extensions: 90
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 89
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 90
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 130061696
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)