BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--1004 (692 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1815.01 |eno101|eno1|enolase|Schizosaccharomyces pombe|chr 2... 116 4e-27 SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase |Schizosa... 101 1e-22 SPBC4B4.10c |mug77|atg5|autophagy associated protein Atg5 |Schiz... 29 0.64 SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 26 4.5 SPBC106.04 |ada1||adenosine deaminase Ada1 |Schizosaccharomyces ... 25 7.9 SPAC4A8.10 |||lipase |Schizosaccharomyces pombe|chr 1|||Manual 25 7.9 >SPBC1815.01 |eno101|eno1|enolase|Schizosaccharomyces pombe|chr 2|||Manual Length = 439 Score = 116 bits (278), Expect = 4e-27 Identities = 50/86 (58%), Positives = 65/86 (75%) Frame = +1 Query: 253 RIGMDVAASEFFKDGKYDLDFKNPDSNPGDYLSSDKLADVYLDFIKDFPMVSIEDPFDQD 432 +IG+DVA+SEF+ DGKYDLD K P + L+ +L D+Y++ K +P+VSIEDPFDQD Sbjct: 242 KIGLDVASSEFYVDGKYDLDIKAAKPKPENKLTYQQLTDLYVELSKKYPIVSIEDPFDQD 301 Query: 433 DWSAWANLTGRTPIQIVGDDLTVTNL 510 DWSAW ++ T QIVGDDLTVTN+ Sbjct: 302 DWSAWTHMKAETDFQIVGDDLTVTNV 327 Score = 110 bits (264), Expect = 2e-25 Identities = 52/86 (60%), Positives = 64/86 (74%) Frame = +2 Query: 2 SHAGNKLAMQEFMIFPTGASTFSEAMRMGSEVYHHLKKIIKEKFGLDSTAVGDEGGFAPN 181 SHAG LA QEFMI PTGA +FSEAMR G+E YH LK I K+++G + VGDEGG AP+ Sbjct: 158 SHAGGDLAFQEFMILPTGAPSFSEAMRWGAETYHTLKSIAKKRYGSSAGNVGDEGGIAPD 217 Query: 182 IQNNKDALYLIQDAIQKAGYAGKIAL 259 +Q ++AL LI +AI KAGY GKI + Sbjct: 218 LQTPQEALDLIVEAINKAGYEGKIKI 243 Score = 49.2 bits (112), Expect = 6e-07 Identities = 22/31 (70%), Positives = 27/31 (87%) Frame = +3 Query: 510 KRIATAVEKKACNCLLLKVNQIGSVTESIDA 602 KR+ TA++KK N LLLKVNQIGSVTES++A Sbjct: 328 KRLRTAIDKKCANALLLKVNQIGSVTESLNA 358 Score = 43.2 bits (97), Expect = 4e-05 Identities = 18/23 (78%), Positives = 19/23 (82%) Frame = +2 Query: 623 GWGTMVSHRSGETEDTFIADLVV 691 GWG MVSHRSGET DTFI+ L V Sbjct: 366 GWGVMVSHRSGETADTFISHLTV 388 >SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase |Schizosaccharomyces pombe|chr 2|||Manual Length = 440 Score = 101 bits (242), Expect = 1e-22 Identities = 46/87 (52%), Positives = 62/87 (71%) Frame = +2 Query: 5 HAGNKLAMQEFMIFPTGASTFSEAMRMGSEVYHHLKKIIKEKFGLDSTAVGDEGGFAPNI 184 HAG LA QEFMI P A TFSE +R GSEVYH LK + K+K+G + VGDEGG AP++ Sbjct: 160 HAGGDLAFQEFMIMPIKAPTFSEGLRWGSEVYHTLKALAKKKYGASAGNVGDEGGIAPDL 219 Query: 185 QNNKDALYLIQDAIQKAGYAGKIALAW 265 ++AL L+ +AI++AGY GK+ + + Sbjct: 220 TTAEEALDLVNEAIKEAGYDGKVKIGF 246 Score = 89.0 bits (211), Expect = 6e-19 Identities = 38/86 (44%), Positives = 58/86 (67%) Frame = +1 Query: 253 RIGMDVAASEFFKDGKYDLDFKNPDSNPGDYLSSDKLADVYLDFIKDFPMVSIEDPFDQD 432 +IG DVAASE + YDLDFK+ P + L KL + Y I+ +P+V IEDPF ++ Sbjct: 243 KIGFDVAASELYNGKLYDLDFKSEHPKPENKLDYKKLYEKYSALIEKYPIVFIEDPFSEE 302 Query: 433 DWSAWANLTGRTPIQIVGDDLTVTNL 510 DW A++ ++ +T ++++ DDLTVTN+ Sbjct: 303 DWGAFSYMSSKTKVEVIADDLTVTNV 328 Score = 46.0 bits (104), Expect = 5e-06 Identities = 22/45 (48%), Positives = 33/45 (73%) Frame = +3 Query: 510 KRIATAVEKKACNCLLLKVNQIGSVTESIDAHLLARRTDGAQWSL 644 KR++ A+E K N LL+K+NQIGS++E+IDA +A++ A W L Sbjct: 329 KRLSKAIELKCANALLVKINQIGSLSETIDAANMAKK---AGWGL 370 Score = 45.2 bits (102), Expect = 9e-06 Identities = 19/26 (73%), Positives = 22/26 (84%) Frame = +2 Query: 614 QKNGWGTMVSHRSGETEDTFIADLVV 691 +K GWG MVSHRSGET+D+FIA L V Sbjct: 364 KKAGWGLMVSHRSGETDDSFIAHLAV 389 >SPBC4B4.10c |mug77|atg5|autophagy associated protein Atg5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 261 Score = 29.1 bits (62), Expect = 0.64 Identities = 10/29 (34%), Positives = 18/29 (62%) Frame = -3 Query: 87 PILMASLKVDAPVGKIMNSCMASLFPACD 1 PI+ S + + +G+ +N + LFP+CD Sbjct: 192 PIIQTSAPLGSSLGEFLNKRLPDLFPSCD 220 >SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1610 Score = 26.2 bits (55), Expect = 4.5 Identities = 16/70 (22%), Positives = 34/70 (48%), Gaps = 2/70 (2%) Frame = -2 Query: 271 LHPCQCDLASIASFLDSILNQIKS--ILVVLYVWSETTFITNSRRVQSKLLLNDLFQMVV 98 L PC L +++ + + + + ++ V +++S+TT I + + K L+NDL ++ Sbjct: 525 LGPCLSFLMKLSTHKNERIACLSASQLITVCHIFSDTTLIKPVKELFQKYLVNDLPSSIL 584 Query: 97 HF*THPHGFT 68 FT Sbjct: 585 KLGPQSPAFT 594 >SPBC106.04 |ada1||adenosine deaminase Ada1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 846 Score = 25.4 bits (53), Expect = 7.9 Identities = 19/54 (35%), Positives = 28/54 (51%), Gaps = 15/54 (27%) Frame = +1 Query: 322 PDSNPG-----DYLSSDKLADVYLDFIKDFPM------VSIE----DPFDQDDW 438 P+++PG D L SD+LA++YL K M VS++ +P D D W Sbjct: 154 PETDPGNMETTDPLFSDELAEIYLSIHKCMDMRHKYIRVSLQGELDNPIDDDSW 207 >SPAC4A8.10 |||lipase |Schizosaccharomyces pombe|chr 1|||Manual Length = 723 Score = 25.4 bits (53), Expect = 7.9 Identities = 18/73 (24%), Positives = 33/73 (45%) Frame = +1 Query: 220 CYPESWLCWQDRIGMDVAASEFFKDGKYDLDFKNPDSNPGDYLSSDKLADVYLDFIKDFP 399 C P S Q I +++A+ FKD ++F+ S + + AD+ D + FP Sbjct: 163 CVPYSCFSNQQPIYIEIASQAIFKD--RSINFELAISQSQHSIRAMSAADI--DTLHAFP 218 Query: 400 MVSIEDPFDQDDW 438 + +E ++ W Sbjct: 219 ALHVEHQTPENLW 231 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,109,110 Number of Sequences: 5004 Number of extensions: 66191 Number of successful extensions: 179 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 170 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 179 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 321951680 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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