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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--1004
         (692 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1815.01 |eno101|eno1|enolase|Schizosaccharomyces pombe|chr 2...   116   4e-27
SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase |Schizosa...   101   1e-22
SPBC4B4.10c |mug77|atg5|autophagy associated protein Atg5 |Schiz...    29   0.64 
SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce...    26   4.5  
SPBC106.04 |ada1||adenosine deaminase Ada1 |Schizosaccharomyces ...    25   7.9  
SPAC4A8.10 |||lipase |Schizosaccharomyces pombe|chr 1|||Manual         25   7.9  

>SPBC1815.01 |eno101|eno1|enolase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 439

 Score =  116 bits (278), Expect = 4e-27
 Identities = 50/86 (58%), Positives = 65/86 (75%)
 Frame = +1

Query: 253 RIGMDVAASEFFKDGKYDLDFKNPDSNPGDYLSSDKLADVYLDFIKDFPMVSIEDPFDQD 432
           +IG+DVA+SEF+ DGKYDLD K     P + L+  +L D+Y++  K +P+VSIEDPFDQD
Sbjct: 242 KIGLDVASSEFYVDGKYDLDIKAAKPKPENKLTYQQLTDLYVELSKKYPIVSIEDPFDQD 301

Query: 433 DWSAWANLTGRTPIQIVGDDLTVTNL 510
           DWSAW ++   T  QIVGDDLTVTN+
Sbjct: 302 DWSAWTHMKAETDFQIVGDDLTVTNV 327



 Score =  110 bits (264), Expect = 2e-25
 Identities = 52/86 (60%), Positives = 64/86 (74%)
 Frame = +2

Query: 2   SHAGNKLAMQEFMIFPTGASTFSEAMRMGSEVYHHLKKIIKEKFGLDSTAVGDEGGFAPN 181
           SHAG  LA QEFMI PTGA +FSEAMR G+E YH LK I K+++G  +  VGDEGG AP+
Sbjct: 158 SHAGGDLAFQEFMILPTGAPSFSEAMRWGAETYHTLKSIAKKRYGSSAGNVGDEGGIAPD 217

Query: 182 IQNNKDALYLIQDAIQKAGYAGKIAL 259
           +Q  ++AL LI +AI KAGY GKI +
Sbjct: 218 LQTPQEALDLIVEAINKAGYEGKIKI 243



 Score = 49.2 bits (112), Expect = 6e-07
 Identities = 22/31 (70%), Positives = 27/31 (87%)
 Frame = +3

Query: 510 KRIATAVEKKACNCLLLKVNQIGSVTESIDA 602
           KR+ TA++KK  N LLLKVNQIGSVTES++A
Sbjct: 328 KRLRTAIDKKCANALLLKVNQIGSVTESLNA 358



 Score = 43.2 bits (97), Expect = 4e-05
 Identities = 18/23 (78%), Positives = 19/23 (82%)
 Frame = +2

Query: 623 GWGTMVSHRSGETEDTFIADLVV 691
           GWG MVSHRSGET DTFI+ L V
Sbjct: 366 GWGVMVSHRSGETADTFISHLTV 388


>SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 440

 Score =  101 bits (242), Expect = 1e-22
 Identities = 46/87 (52%), Positives = 62/87 (71%)
 Frame = +2

Query: 5   HAGNKLAMQEFMIFPTGASTFSEAMRMGSEVYHHLKKIIKEKFGLDSTAVGDEGGFAPNI 184
           HAG  LA QEFMI P  A TFSE +R GSEVYH LK + K+K+G  +  VGDEGG AP++
Sbjct: 160 HAGGDLAFQEFMIMPIKAPTFSEGLRWGSEVYHTLKALAKKKYGASAGNVGDEGGIAPDL 219

Query: 185 QNNKDALYLIQDAIQKAGYAGKIALAW 265
              ++AL L+ +AI++AGY GK+ + +
Sbjct: 220 TTAEEALDLVNEAIKEAGYDGKVKIGF 246



 Score = 89.0 bits (211), Expect = 6e-19
 Identities = 38/86 (44%), Positives = 58/86 (67%)
 Frame = +1

Query: 253 RIGMDVAASEFFKDGKYDLDFKNPDSNPGDYLSSDKLADVYLDFIKDFPMVSIEDPFDQD 432
           +IG DVAASE +    YDLDFK+    P + L   KL + Y   I+ +P+V IEDPF ++
Sbjct: 243 KIGFDVAASELYNGKLYDLDFKSEHPKPENKLDYKKLYEKYSALIEKYPIVFIEDPFSEE 302

Query: 433 DWSAWANLTGRTPIQIVGDDLTVTNL 510
           DW A++ ++ +T ++++ DDLTVTN+
Sbjct: 303 DWGAFSYMSSKTKVEVIADDLTVTNV 328



 Score = 46.0 bits (104), Expect = 5e-06
 Identities = 22/45 (48%), Positives = 33/45 (73%)
 Frame = +3

Query: 510 KRIATAVEKKACNCLLLKVNQIGSVTESIDAHLLARRTDGAQWSL 644
           KR++ A+E K  N LL+K+NQIGS++E+IDA  +A++   A W L
Sbjct: 329 KRLSKAIELKCANALLVKINQIGSLSETIDAANMAKK---AGWGL 370



 Score = 45.2 bits (102), Expect = 9e-06
 Identities = 19/26 (73%), Positives = 22/26 (84%)
 Frame = +2

Query: 614 QKNGWGTMVSHRSGETEDTFIADLVV 691
           +K GWG MVSHRSGET+D+FIA L V
Sbjct: 364 KKAGWGLMVSHRSGETDDSFIAHLAV 389


>SPBC4B4.10c |mug77|atg5|autophagy associated protein Atg5
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 261

 Score = 29.1 bits (62), Expect = 0.64
 Identities = 10/29 (34%), Positives = 18/29 (62%)
 Frame = -3

Query: 87  PILMASLKVDAPVGKIMNSCMASLFPACD 1
           PI+  S  + + +G+ +N  +  LFP+CD
Sbjct: 192 PIIQTSAPLGSSLGEFLNKRLPDLFPSCD 220


>SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1610

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 16/70 (22%), Positives = 34/70 (48%), Gaps = 2/70 (2%)
 Frame = -2

Query: 271 LHPCQCDLASIASFLDSILNQIKS--ILVVLYVWSETTFITNSRRVQSKLLLNDLFQMVV 98
           L PC   L  +++  +  +  + +  ++ V +++S+TT I   + +  K L+NDL   ++
Sbjct: 525 LGPCLSFLMKLSTHKNERIACLSASQLITVCHIFSDTTLIKPVKELFQKYLVNDLPSSIL 584

Query: 97  HF*THPHGFT 68
                   FT
Sbjct: 585 KLGPQSPAFT 594


>SPBC106.04 |ada1||adenosine deaminase Ada1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 846

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 19/54 (35%), Positives = 28/54 (51%), Gaps = 15/54 (27%)
 Frame = +1

Query: 322 PDSNPG-----DYLSSDKLADVYLDFIKDFPM------VSIE----DPFDQDDW 438
           P+++PG     D L SD+LA++YL   K   M      VS++    +P D D W
Sbjct: 154 PETDPGNMETTDPLFSDELAEIYLSIHKCMDMRHKYIRVSLQGELDNPIDDDSW 207


>SPAC4A8.10 |||lipase |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 723

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 18/73 (24%), Positives = 33/73 (45%)
 Frame = +1

Query: 220 CYPESWLCWQDRIGMDVAASEFFKDGKYDLDFKNPDSNPGDYLSSDKLADVYLDFIKDFP 399
           C P S    Q  I +++A+   FKD    ++F+   S     + +   AD+  D +  FP
Sbjct: 163 CVPYSCFSNQQPIYIEIASQAIFKD--RSINFELAISQSQHSIRAMSAADI--DTLHAFP 218

Query: 400 MVSIEDPFDQDDW 438
            + +E    ++ W
Sbjct: 219 ALHVEHQTPENLW 231


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,109,110
Number of Sequences: 5004
Number of extensions: 66191
Number of successful extensions: 179
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 179
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 321951680
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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