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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--1004
         (692 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g36530.1 68415.m04481 enolase identical to SWISS-PROT:P25696 ...   137   7e-33
At1g74030.1 68414.m08573 enolase, putative similar to Swiss-Prot...   129   1e-30
At2g29560.1 68415.m03590 enolase, putative similar to enolase [S...    95   5e-20
At3g49490.1 68416.m05409 expressed protein                             33   0.14 
At5g08530.1 68418.m01013 NADH-ubiquinone oxidoreductase 51 kDa s...    29   2.9  
At1g14140.1 68414.m01671 mitochondrial substrate carrier family ...    29   2.9  
At3g59020.1 68416.m06578 importin beta-2 subunit family protein ...    29   3.9  
At1g26370.1 68414.m03217 RNA helicase, putative similar to SP|Q1...    29   3.9  
At5g16290.2 68418.m01904 acetolactate synthase small subunit, pu...    28   6.8  
At5g16290.1 68418.m01903 acetolactate synthase small subunit, pu...    28   6.8  
At2g44950.1 68415.m05596 zinc finger (C3HC4-type RING finger) fa...    27   8.9  

>At2g36530.1 68415.m04481 enolase identical to SWISS-PROT:P25696
           enolase (EC 4.2.1.11) (2-phosphoglycerate
           dehydratase)(2-phospho-D- glycerate hydro-lyase)
           [Arabidopsis thaliana]
          Length = 444

 Score =  137 bits (331), Expect = 7e-33
 Identities = 60/86 (69%), Positives = 73/86 (84%)
 Frame = +2

Query: 2   SHAGNKLAMQEFMIFPTGASTFSEAMRMGSEVYHHLKKIIKEKFGLDSTAVGDEGGFAPN 181
           SHAGNKLAMQEFMI P GA++F EAM+MG EVYHHLK +IK+K+G D+T VGDEGGFAPN
Sbjct: 162 SHAGNKLAMQEFMILPVGAASFKEAMKMGVEVYHHLKSVIKKKYGQDATNVGDEGGFAPN 221

Query: 182 IQNNKDALYLIQDAIQKAGYAGKIAL 259
           IQ NK+ L L++ AI+KAGY GK+ +
Sbjct: 222 IQENKEGLELLKTAIEKAGYTGKVVI 247



 Score =  103 bits (247), Expect = 1e-22
 Identities = 49/87 (56%), Positives = 63/87 (72%), Gaps = 3/87 (3%)
 Frame = +1

Query: 256 IGMDVAASEFFKDGK-YDLDFKNPDSNPGDYLSSDKLADVYLDFIKDFPMVSIEDPFDQD 432
           IGMDVAASEF+ + K YDL+FK  ++N    +S D L D+Y  F+ ++P+VSIEDPFDQD
Sbjct: 247 IGMDVAASEFYSEDKTYDLNFKEENNNGSQKISGDALKDLYKSFVAEYPIVSIEDPFDQD 306

Query: 433 DWSAWANLTGR--TPIQIVGDDLTVTN 507
           DW  +A +T    T +QIVGDDL VTN
Sbjct: 307 DWEHYAKMTTECGTEVQIVGDDLLVTN 333



 Score = 55.2 bits (127), Expect = 4e-08
 Identities = 24/38 (63%), Positives = 33/38 (86%)
 Frame = +3

Query: 507 PKRIATAVEKKACNCLLLKVNQIGSVTESIDAHLLARR 620
           PKR+A A+ +K+CN LLLKVNQIGSVTESI+A  ++++
Sbjct: 334 PKRVAKAIAEKSCNALLLKVNQIGSVTESIEAVKMSKK 371



 Score = 49.6 bits (113), Expect = 2e-06
 Identities = 21/26 (80%), Positives = 22/26 (84%)
 Frame = +2

Query: 614 QKNGWGTMVSHRSGETEDTFIADLVV 691
           +K GWG M SHRSGETEDTFIADL V
Sbjct: 370 KKAGWGVMTSHRSGETEDTFIADLAV 395


>At1g74030.1 68414.m08573 enolase, putative similar to
           Swiss-Prot:P15007 enolase (EC 4.2.1.11)
           (2-phosphoglycerate dehydratase)(2-phospho-D- glycerate
           hydro-lyase) [Drosophila melanogaster]
          Length = 477

 Score =  129 bits (312), Expect = 1e-30
 Identities = 59/86 (68%), Positives = 71/86 (82%)
 Frame = +2

Query: 2   SHAGNKLAMQEFMIFPTGASTFSEAMRMGSEVYHHLKKIIKEKFGLDSTAVGDEGGFAPN 181
           SHAGN LAMQEFMI P GA++FSEA +MGSEVYH LK IIK K+G D+  VGDEGGFAPN
Sbjct: 202 SHAGNSLAMQEFMILPVGATSFSEAFQMGSEVYHTLKGIIKTKYGQDACNVGDEGGFAPN 261

Query: 182 IQNNKDALYLIQDAIQKAGYAGKIAL 259
           +Q+N++ L L+ DAI+KAGY GKI +
Sbjct: 262 VQDNREGLVLLIDAIEKAGYTGKIKI 287



 Score =  124 bits (300), Expect = 4e-29
 Identities = 57/86 (66%), Positives = 70/86 (81%), Gaps = 1/86 (1%)
 Frame = +1

Query: 253 RIGMDVAASEFF-KDGKYDLDFKNPDSNPGDYLSSDKLADVYLDFIKDFPMVSIEDPFDQ 429
           +IGMDVAASEFF KDG+YDL+FK   ++    LS++ LAD+Y +FIKDFP+VSIEDPFDQ
Sbjct: 286 KIGMDVAASEFFMKDGRYDLNFKKQPNDGAHVLSAESLADLYREFIKDFPIVSIEDPFDQ 345

Query: 430 DDWSAWANLTGRTPIQIVGDDLTVTN 507
           DDWS+WA+L     IQ+VGDDL VTN
Sbjct: 346 DDWSSWASLQSSVDIQLVGDDLLVTN 371



 Score = 54.4 bits (125), Expect = 7e-08
 Identities = 25/34 (73%), Positives = 30/34 (88%)
 Frame = +3

Query: 507 PKRIATAVEKKACNCLLLKVNQIGSVTESIDAHL 608
           PKRIA A++K++CN LLLKVNQIG+VTESI A L
Sbjct: 372 PKRIAEAIKKQSCNALLLKVNQIGTVTESIQAAL 405



 Score = 47.2 bits (107), Expect = 1e-05
 Identities = 21/30 (70%), Positives = 23/30 (76%)
 Frame = +2

Query: 602 SLAGQKNGWGTMVSHRSGETEDTFIADLVV 691
           +L  +  GWG MVSHRSGETED FIADL V
Sbjct: 404 ALDSKAAGWGVMVSHRSGETEDNFIADLSV 433


>At2g29560.1 68415.m03590 enolase, putative similar to enolase
           [Spinacia oleracea] gi|8919731|emb|CAB96173
          Length = 475

 Score = 94.7 bits (225), Expect = 5e-20
 Identities = 44/86 (51%), Positives = 58/86 (67%)
 Frame = +2

Query: 5   HAGNKLAMQEFMIFPTGASTFSEAMRMGSEVYHHLKKIIKEKFGLDSTAVGDEGGFAPNI 184
           HA N  A+QE MI P GAS F EA++ GSE YHHLK +I EK G     VG++GG AP+I
Sbjct: 200 HASNTFAIQEIMILPIGASRFEEALQWGSETYHHLKAVISEKNGGLGCNVGEDGGLAPDI 259

Query: 185 QNNKDALYLIQDAIQKAGYAGKIALA 262
            + K+ L L+++AI + GY  KI +A
Sbjct: 260 SSLKEGLELVKEAINRTGYNDKIKIA 285



 Score = 81.0 bits (191), Expect = 7e-16
 Identities = 34/85 (40%), Positives = 56/85 (65%)
 Frame = +1

Query: 253 RIGMDVAASEFFKDGKYDLDFKNPDSNPGDYLSSDKLADVYLDFIKDFPMVSIEDPFDQD 432
           +I +D+AA+ F    KYDLD K+P+ +  ++ S++ + D+Y +   D+P+VSIEDPFD++
Sbjct: 283 KIAIDIAATNFCLGTKYDLDIKSPNKSGQNFKSAEDMIDMYKEICNDYPIVSIEDPFDKE 342

Query: 433 DWSAWANLTGRTPIQIVGDDLTVTN 507
           DW      +     Q+VGDDL ++N
Sbjct: 343 DWEHTKYFSSLGICQVVGDDLLMSN 367



 Score = 49.2 bits (112), Expect = 3e-06
 Identities = 23/45 (51%), Positives = 33/45 (73%)
 Frame = +3

Query: 510 KRIATAVEKKACNCLLLKVNQIGSVTESIDAHLLARRTDGAQWSL 644
           KR+  A+++ +CN LLLKVNQIG+VTE+I+   +AR    AQW +
Sbjct: 369 KRVERAIQESSCNALLLKVNQIGTVTEAIEVVKMAR---DAQWGV 410



 Score = 38.3 bits (85), Expect = 0.005
 Identities = 15/22 (68%), Positives = 18/22 (81%)
 Frame = +2

Query: 626 WGTMVSHRSGETEDTFIADLVV 691
           WG + SHR GETED+FI+DL V
Sbjct: 408 WGVVTSHRCGETEDSFISDLSV 429


>At3g49490.1 68416.m05409 expressed protein
          Length = 953

 Score = 33.5 bits (73), Expect = 0.14
 Identities = 19/48 (39%), Positives = 27/48 (56%)
 Frame = -2

Query: 691 NYQVGNKGIFGLTRSVRDHCAPSVLLASK*ASIDSVTLPI*LTFNSRQ 548
           N Q GN G+ G   S +D  +PS   +S+ +S D V+LP    F+ RQ
Sbjct: 93  NTQHGNSGLRGEDSSYKDMLSPSGFYSSRESSSDFVSLPPTENFSERQ 140


>At5g08530.1 68418.m01013 NADH-ubiquinone oxidoreductase 51 kDa
           subunit, mitochondrial, putative similar to
           NADH-ubiquinone oxidoreductase 51 kDa subunit,
           mitochondrial precursor (EC 1.6.5.3) (EC 1.6.99.3) from
           {Homo sapiens} SP|P49821, {Bos taurus} SP|P25708,
           {Aspergillus niger} SP|Q92406; contains Pfam profile
           PF01512: Respiratory-chain NADH dehydrogenase 51 Kd
           subunit
          Length = 486

 Score = 29.1 bits (62), Expect = 2.9
 Identities = 12/41 (29%), Positives = 21/41 (51%)
 Frame = +3

Query: 537 KACNCLLLKVNQIGSVTESIDAHLLARRTDGAQWSLTDLVR 659
           K  N  L +++ +  VT+ I+ H +    D A W +  L+R
Sbjct: 423 KVGNAKLEEIDMLQEVTKQIEGHTICALGDAAAWPVQGLIR 463


>At1g14140.1 68414.m01671 mitochondrial substrate carrier family
           protein contains Pfam profile: PF00153 mitochondrial
           carrier protein
          Length = 305

 Score = 29.1 bits (62), Expect = 2.9
 Identities = 19/40 (47%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
 Frame = -2

Query: 622 VLLASK*ASI-DSVTLPI*LTFNSRQLHAFFSTAVAIRLG 506
           +LLAS  A + +SVT PI LT    QLH   S + A R+G
Sbjct: 16  ILLASLSAMVAESVTFPIDLTKTRMQLHGSGSASGAHRIG 55


>At3g59020.1 68416.m06578 importin beta-2 subunit family protein
            similar to D-Importin 7/RanBP7 [Drosophila melanogaster]
            GI:7542336; contains Pfam profile PF03810: Importin-beta
            N-terminal domain
          Length = 1112

 Score = 28.7 bits (61), Expect = 3.9
 Identities = 25/66 (37%), Positives = 30/66 (45%), Gaps = 1/66 (1%)
 Frame = +1

Query: 247  QDRIGMDVAASEFFKDGK-YDLDFKNPDSNPGDYLSSDKLADVYLDFIKDFPMVSIEDPF 423
            +D  G D    EF  D +  D D +NPD   G  L   KLA       KDF   S +D F
Sbjct: 976  EDEDGDDDDMDEFQTDDEDEDGDDENPDETDGSTLR--KLAAQ----AKDFRSYSDDDDF 1029

Query: 424  DQDDWS 441
              DD+S
Sbjct: 1030 SDDDFS 1035


>At1g26370.1 68414.m03217 RNA helicase, putative similar to
           SP|Q14562 ATP-dependent helicase DDX8 (RNA helicase
           HRH1) (DEAH-box protein 8) {Homo sapiens}; contains Pfam
           profiles PF04408: Helicase associated domain (HA2),
           PF00271: Helicase conserved C-terminal domain
          Length = 717

 Score = 28.7 bits (61), Expect = 3.9
 Identities = 15/36 (41%), Positives = 21/36 (58%)
 Frame = -1

Query: 332 LESGFLKSRSYFPSLKNSEAATSMPMRSCQHSQLSG 225
           ++ GF+K+RSY PS K  E+   +P    Q  Q SG
Sbjct: 373 IDPGFVKARSYDPS-KGMESLDVVPASKAQTLQRSG 407


>At5g16290.2 68418.m01904 acetolactate synthase small subunit,
           putative similar to gi:5931761 from Nicotiana
           plumbaginifolia
          Length = 477

 Score = 27.9 bits (59), Expect = 6.8
 Identities = 12/36 (33%), Positives = 19/36 (52%)
 Frame = -3

Query: 489 ITNNLNRRATSEVSPCRPIILVKRILNGHHGKIFDE 382
           +T N N  +  +V P  P    K +L+ H G ++DE
Sbjct: 265 LTGNGNASSGGDVYPVEPYNDFKPVLDAHWGMVYDE 300


>At5g16290.1 68418.m01903 acetolactate synthase small subunit,
           putative similar to gi:5931761 from Nicotiana
           plumbaginifolia
          Length = 477

 Score = 27.9 bits (59), Expect = 6.8
 Identities = 12/36 (33%), Positives = 19/36 (52%)
 Frame = -3

Query: 489 ITNNLNRRATSEVSPCRPIILVKRILNGHHGKIFDE 382
           +T N N  +  +V P  P    K +L+ H G ++DE
Sbjct: 265 LTGNGNASSGGDVYPVEPYNDFKPVLDAHWGMVYDE 300


>At2g44950.1 68415.m05596 zinc finger (C3HC4-type RING finger)
           family protein contains Pfam profile: PF00097 zinc
           finger, C3HC4 type (RING finger)
          Length = 878

 Score = 27.5 bits (58), Expect = 8.9
 Identities = 12/26 (46%), Positives = 17/26 (65%), Gaps = 1/26 (3%)
 Frame = -3

Query: 666 SSVSP-DL*ETIVPHPFFWPASEHQL 592
           SS+SP +    +   PFFWP+SE +L
Sbjct: 15  SSISPSEAAAAVKKQPFFWPSSEDKL 40


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,620,532
Number of Sequences: 28952
Number of extensions: 360723
Number of successful extensions: 995
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 958
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 991
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1477286152
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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