BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--1004 (692 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g36530.1 68415.m04481 enolase identical to SWISS-PROT:P25696 ... 137 7e-33 At1g74030.1 68414.m08573 enolase, putative similar to Swiss-Prot... 129 1e-30 At2g29560.1 68415.m03590 enolase, putative similar to enolase [S... 95 5e-20 At3g49490.1 68416.m05409 expressed protein 33 0.14 At5g08530.1 68418.m01013 NADH-ubiquinone oxidoreductase 51 kDa s... 29 2.9 At1g14140.1 68414.m01671 mitochondrial substrate carrier family ... 29 2.9 At3g59020.1 68416.m06578 importin beta-2 subunit family protein ... 29 3.9 At1g26370.1 68414.m03217 RNA helicase, putative similar to SP|Q1... 29 3.9 At5g16290.2 68418.m01904 acetolactate synthase small subunit, pu... 28 6.8 At5g16290.1 68418.m01903 acetolactate synthase small subunit, pu... 28 6.8 At2g44950.1 68415.m05596 zinc finger (C3HC4-type RING finger) fa... 27 8.9 >At2g36530.1 68415.m04481 enolase identical to SWISS-PROT:P25696 enolase (EC 4.2.1.11) (2-phosphoglycerate dehydratase)(2-phospho-D- glycerate hydro-lyase) [Arabidopsis thaliana] Length = 444 Score = 137 bits (331), Expect = 7e-33 Identities = 60/86 (69%), Positives = 73/86 (84%) Frame = +2 Query: 2 SHAGNKLAMQEFMIFPTGASTFSEAMRMGSEVYHHLKKIIKEKFGLDSTAVGDEGGFAPN 181 SHAGNKLAMQEFMI P GA++F EAM+MG EVYHHLK +IK+K+G D+T VGDEGGFAPN Sbjct: 162 SHAGNKLAMQEFMILPVGAASFKEAMKMGVEVYHHLKSVIKKKYGQDATNVGDEGGFAPN 221 Query: 182 IQNNKDALYLIQDAIQKAGYAGKIAL 259 IQ NK+ L L++ AI+KAGY GK+ + Sbjct: 222 IQENKEGLELLKTAIEKAGYTGKVVI 247 Score = 103 bits (247), Expect = 1e-22 Identities = 49/87 (56%), Positives = 63/87 (72%), Gaps = 3/87 (3%) Frame = +1 Query: 256 IGMDVAASEFFKDGK-YDLDFKNPDSNPGDYLSSDKLADVYLDFIKDFPMVSIEDPFDQD 432 IGMDVAASEF+ + K YDL+FK ++N +S D L D+Y F+ ++P+VSIEDPFDQD Sbjct: 247 IGMDVAASEFYSEDKTYDLNFKEENNNGSQKISGDALKDLYKSFVAEYPIVSIEDPFDQD 306 Query: 433 DWSAWANLTGR--TPIQIVGDDLTVTN 507 DW +A +T T +QIVGDDL VTN Sbjct: 307 DWEHYAKMTTECGTEVQIVGDDLLVTN 333 Score = 55.2 bits (127), Expect = 4e-08 Identities = 24/38 (63%), Positives = 33/38 (86%) Frame = +3 Query: 507 PKRIATAVEKKACNCLLLKVNQIGSVTESIDAHLLARR 620 PKR+A A+ +K+CN LLLKVNQIGSVTESI+A ++++ Sbjct: 334 PKRVAKAIAEKSCNALLLKVNQIGSVTESIEAVKMSKK 371 Score = 49.6 bits (113), Expect = 2e-06 Identities = 21/26 (80%), Positives = 22/26 (84%) Frame = +2 Query: 614 QKNGWGTMVSHRSGETEDTFIADLVV 691 +K GWG M SHRSGETEDTFIADL V Sbjct: 370 KKAGWGVMTSHRSGETEDTFIADLAV 395 >At1g74030.1 68414.m08573 enolase, putative similar to Swiss-Prot:P15007 enolase (EC 4.2.1.11) (2-phosphoglycerate dehydratase)(2-phospho-D- glycerate hydro-lyase) [Drosophila melanogaster] Length = 477 Score = 129 bits (312), Expect = 1e-30 Identities = 59/86 (68%), Positives = 71/86 (82%) Frame = +2 Query: 2 SHAGNKLAMQEFMIFPTGASTFSEAMRMGSEVYHHLKKIIKEKFGLDSTAVGDEGGFAPN 181 SHAGN LAMQEFMI P GA++FSEA +MGSEVYH LK IIK K+G D+ VGDEGGFAPN Sbjct: 202 SHAGNSLAMQEFMILPVGATSFSEAFQMGSEVYHTLKGIIKTKYGQDACNVGDEGGFAPN 261 Query: 182 IQNNKDALYLIQDAIQKAGYAGKIAL 259 +Q+N++ L L+ DAI+KAGY GKI + Sbjct: 262 VQDNREGLVLLIDAIEKAGYTGKIKI 287 Score = 124 bits (300), Expect = 4e-29 Identities = 57/86 (66%), Positives = 70/86 (81%), Gaps = 1/86 (1%) Frame = +1 Query: 253 RIGMDVAASEFF-KDGKYDLDFKNPDSNPGDYLSSDKLADVYLDFIKDFPMVSIEDPFDQ 429 +IGMDVAASEFF KDG+YDL+FK ++ LS++ LAD+Y +FIKDFP+VSIEDPFDQ Sbjct: 286 KIGMDVAASEFFMKDGRYDLNFKKQPNDGAHVLSAESLADLYREFIKDFPIVSIEDPFDQ 345 Query: 430 DDWSAWANLTGRTPIQIVGDDLTVTN 507 DDWS+WA+L IQ+VGDDL VTN Sbjct: 346 DDWSSWASLQSSVDIQLVGDDLLVTN 371 Score = 54.4 bits (125), Expect = 7e-08 Identities = 25/34 (73%), Positives = 30/34 (88%) Frame = +3 Query: 507 PKRIATAVEKKACNCLLLKVNQIGSVTESIDAHL 608 PKRIA A++K++CN LLLKVNQIG+VTESI A L Sbjct: 372 PKRIAEAIKKQSCNALLLKVNQIGTVTESIQAAL 405 Score = 47.2 bits (107), Expect = 1e-05 Identities = 21/30 (70%), Positives = 23/30 (76%) Frame = +2 Query: 602 SLAGQKNGWGTMVSHRSGETEDTFIADLVV 691 +L + GWG MVSHRSGETED FIADL V Sbjct: 404 ALDSKAAGWGVMVSHRSGETEDNFIADLSV 433 >At2g29560.1 68415.m03590 enolase, putative similar to enolase [Spinacia oleracea] gi|8919731|emb|CAB96173 Length = 475 Score = 94.7 bits (225), Expect = 5e-20 Identities = 44/86 (51%), Positives = 58/86 (67%) Frame = +2 Query: 5 HAGNKLAMQEFMIFPTGASTFSEAMRMGSEVYHHLKKIIKEKFGLDSTAVGDEGGFAPNI 184 HA N A+QE MI P GAS F EA++ GSE YHHLK +I EK G VG++GG AP+I Sbjct: 200 HASNTFAIQEIMILPIGASRFEEALQWGSETYHHLKAVISEKNGGLGCNVGEDGGLAPDI 259 Query: 185 QNNKDALYLIQDAIQKAGYAGKIALA 262 + K+ L L+++AI + GY KI +A Sbjct: 260 SSLKEGLELVKEAINRTGYNDKIKIA 285 Score = 81.0 bits (191), Expect = 7e-16 Identities = 34/85 (40%), Positives = 56/85 (65%) Frame = +1 Query: 253 RIGMDVAASEFFKDGKYDLDFKNPDSNPGDYLSSDKLADVYLDFIKDFPMVSIEDPFDQD 432 +I +D+AA+ F KYDLD K+P+ + ++ S++ + D+Y + D+P+VSIEDPFD++ Sbjct: 283 KIAIDIAATNFCLGTKYDLDIKSPNKSGQNFKSAEDMIDMYKEICNDYPIVSIEDPFDKE 342 Query: 433 DWSAWANLTGRTPIQIVGDDLTVTN 507 DW + Q+VGDDL ++N Sbjct: 343 DWEHTKYFSSLGICQVVGDDLLMSN 367 Score = 49.2 bits (112), Expect = 3e-06 Identities = 23/45 (51%), Positives = 33/45 (73%) Frame = +3 Query: 510 KRIATAVEKKACNCLLLKVNQIGSVTESIDAHLLARRTDGAQWSL 644 KR+ A+++ +CN LLLKVNQIG+VTE+I+ +AR AQW + Sbjct: 369 KRVERAIQESSCNALLLKVNQIGTVTEAIEVVKMAR---DAQWGV 410 Score = 38.3 bits (85), Expect = 0.005 Identities = 15/22 (68%), Positives = 18/22 (81%) Frame = +2 Query: 626 WGTMVSHRSGETEDTFIADLVV 691 WG + SHR GETED+FI+DL V Sbjct: 408 WGVVTSHRCGETEDSFISDLSV 429 >At3g49490.1 68416.m05409 expressed protein Length = 953 Score = 33.5 bits (73), Expect = 0.14 Identities = 19/48 (39%), Positives = 27/48 (56%) Frame = -2 Query: 691 NYQVGNKGIFGLTRSVRDHCAPSVLLASK*ASIDSVTLPI*LTFNSRQ 548 N Q GN G+ G S +D +PS +S+ +S D V+LP F+ RQ Sbjct: 93 NTQHGNSGLRGEDSSYKDMLSPSGFYSSRESSSDFVSLPPTENFSERQ 140 >At5g08530.1 68418.m01013 NADH-ubiquinone oxidoreductase 51 kDa subunit, mitochondrial, putative similar to NADH-ubiquinone oxidoreductase 51 kDa subunit, mitochondrial precursor (EC 1.6.5.3) (EC 1.6.99.3) from {Homo sapiens} SP|P49821, {Bos taurus} SP|P25708, {Aspergillus niger} SP|Q92406; contains Pfam profile PF01512: Respiratory-chain NADH dehydrogenase 51 Kd subunit Length = 486 Score = 29.1 bits (62), Expect = 2.9 Identities = 12/41 (29%), Positives = 21/41 (51%) Frame = +3 Query: 537 KACNCLLLKVNQIGSVTESIDAHLLARRTDGAQWSLTDLVR 659 K N L +++ + VT+ I+ H + D A W + L+R Sbjct: 423 KVGNAKLEEIDMLQEVTKQIEGHTICALGDAAAWPVQGLIR 463 >At1g14140.1 68414.m01671 mitochondrial substrate carrier family protein contains Pfam profile: PF00153 mitochondrial carrier protein Length = 305 Score = 29.1 bits (62), Expect = 2.9 Identities = 19/40 (47%), Positives = 24/40 (60%), Gaps = 1/40 (2%) Frame = -2 Query: 622 VLLASK*ASI-DSVTLPI*LTFNSRQLHAFFSTAVAIRLG 506 +LLAS A + +SVT PI LT QLH S + A R+G Sbjct: 16 ILLASLSAMVAESVTFPIDLTKTRMQLHGSGSASGAHRIG 55 >At3g59020.1 68416.m06578 importin beta-2 subunit family protein similar to D-Importin 7/RanBP7 [Drosophila melanogaster] GI:7542336; contains Pfam profile PF03810: Importin-beta N-terminal domain Length = 1112 Score = 28.7 bits (61), Expect = 3.9 Identities = 25/66 (37%), Positives = 30/66 (45%), Gaps = 1/66 (1%) Frame = +1 Query: 247 QDRIGMDVAASEFFKDGK-YDLDFKNPDSNPGDYLSSDKLADVYLDFIKDFPMVSIEDPF 423 +D G D EF D + D D +NPD G L KLA KDF S +D F Sbjct: 976 EDEDGDDDDMDEFQTDDEDEDGDDENPDETDGSTLR--KLAAQ----AKDFRSYSDDDDF 1029 Query: 424 DQDDWS 441 DD+S Sbjct: 1030 SDDDFS 1035 >At1g26370.1 68414.m03217 RNA helicase, putative similar to SP|Q14562 ATP-dependent helicase DDX8 (RNA helicase HRH1) (DEAH-box protein 8) {Homo sapiens}; contains Pfam profiles PF04408: Helicase associated domain (HA2), PF00271: Helicase conserved C-terminal domain Length = 717 Score = 28.7 bits (61), Expect = 3.9 Identities = 15/36 (41%), Positives = 21/36 (58%) Frame = -1 Query: 332 LESGFLKSRSYFPSLKNSEAATSMPMRSCQHSQLSG 225 ++ GF+K+RSY PS K E+ +P Q Q SG Sbjct: 373 IDPGFVKARSYDPS-KGMESLDVVPASKAQTLQRSG 407 >At5g16290.2 68418.m01904 acetolactate synthase small subunit, putative similar to gi:5931761 from Nicotiana plumbaginifolia Length = 477 Score = 27.9 bits (59), Expect = 6.8 Identities = 12/36 (33%), Positives = 19/36 (52%) Frame = -3 Query: 489 ITNNLNRRATSEVSPCRPIILVKRILNGHHGKIFDE 382 +T N N + +V P P K +L+ H G ++DE Sbjct: 265 LTGNGNASSGGDVYPVEPYNDFKPVLDAHWGMVYDE 300 >At5g16290.1 68418.m01903 acetolactate synthase small subunit, putative similar to gi:5931761 from Nicotiana plumbaginifolia Length = 477 Score = 27.9 bits (59), Expect = 6.8 Identities = 12/36 (33%), Positives = 19/36 (52%) Frame = -3 Query: 489 ITNNLNRRATSEVSPCRPIILVKRILNGHHGKIFDE 382 +T N N + +V P P K +L+ H G ++DE Sbjct: 265 LTGNGNASSGGDVYPVEPYNDFKPVLDAHWGMVYDE 300 >At2g44950.1 68415.m05596 zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) Length = 878 Score = 27.5 bits (58), Expect = 8.9 Identities = 12/26 (46%), Positives = 17/26 (65%), Gaps = 1/26 (3%) Frame = -3 Query: 666 SSVSP-DL*ETIVPHPFFWPASEHQL 592 SS+SP + + PFFWP+SE +L Sbjct: 15 SSISPSEAAAAVKKQPFFWPSSEDKL 40 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,620,532 Number of Sequences: 28952 Number of extensions: 360723 Number of successful extensions: 995 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 958 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 991 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1477286152 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
- SilkBase 1999-2023 -