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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--0994
         (618 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3 |Schi...    38   9e-04
SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc...    37   0.002
SPBC13E7.02 |cwf24||GCN5-related N acetyltransferase|Schizosacch...    35   0.011
SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces...    33   0.033
SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ...    33   0.044
SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pom...    33   0.044
SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces pomb...    30   0.31 
SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8 |Schizosacch...    29   0.41 
SPAC6B12.07c |||ubiquitin-protein ligase E3 |Schizosaccharomyces...    27   1.6  
SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit 1|Schizosacchar...    27   2.9  
SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch...    27   2.9  
SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p...    27   2.9  
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc...    26   5.0  
SPAC29B12.10c |||OPT oligopeptide transporter family|Schizosacch...    26   5.0  
SPBC30D10.05c |||sepiapterin reductase |Schizosaccharomyces pomb...    25   8.8  

>SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 306

 Score = 38.3 bits (85), Expect = 9e-04
 Identities = 14/43 (32%), Positives = 23/43 (53%)
 Frame = -3

Query: 388 KKHLDQLKEIPNEVDNEKTCVACLEEILQPCVLQCGHIFCHQC 260
           ++ L+   ++P   +  + C  C+E I  P   +CGHIFC  C
Sbjct: 237 ERDLEDKNKLPFIPEGNRKCSLCMEFIHCPAATECGHIFCWSC 279


>SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 732

 Score = 37.1 bits (82), Expect = 0.002
 Identities = 17/40 (42%), Positives = 21/40 (52%)
 Frame = -3

Query: 379 LDQLKEIPNEVDNEKTCVACLEEILQPCVLQCGHIFCHQC 260
           +D  KE  +E  +E+ C  C E I   CVL C H  CH C
Sbjct: 67  VDASKEEQDE--DEQICFICAEGITYSCVLPCNHRMCHVC 104


>SPBC13E7.02 |cwf24||GCN5-related N
           acetyltransferase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 533

 Score = 34.7 bits (76), Expect = 0.011
 Identities = 19/54 (35%), Positives = 25/54 (46%), Gaps = 4/54 (7%)
 Frame = -3

Query: 409 WQSVYXAKKHLDQLKE--IPNEV--DNEKTCVACLEEILQPCVLQCGHIFCHQC 260
           W SV    K   +L+E  + NE   D    C+ C ++   P    CGH FC QC
Sbjct: 224 WDSVQEKYKKGAKLEEGMVKNEKKEDIPFVCLICKKDYRSPIATTCGHHFCEQC 277


>SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 486

 Score = 33.1 bits (72), Expect = 0.033
 Identities = 14/41 (34%), Positives = 18/41 (43%)
 Frame = -3

Query: 382 HLDQLKEIPNEVDNEKTCVACLEEILQPCVLQCGHIFCHQC 260
           H++ L  +   V  E  C  C   +  P V  CGH FC  C
Sbjct: 154 HMEDL--LQENVSRELECQICFGMLYDPVVSPCGHTFCGPC 192


>SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 468

 Score = 32.7 bits (71), Expect = 0.044
 Identities = 11/37 (29%), Positives = 18/37 (48%)
 Frame = -3

Query: 370 LKEIPNEVDNEKTCVACLEEILQPCVLQCGHIFCHQC 260
           L E   ++     C  C E + +P    CGH +C++C
Sbjct: 72  LLETFQKIKKTLECPICTEALQRPFTTHCGHTYCYEC 108


>SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1375

 Score = 32.7 bits (71), Expect = 0.044
 Identities = 13/44 (29%), Positives = 25/44 (56%), Gaps = 1/44 (2%)
 Frame = -3

Query: 388  KKHLDQLKE-IPNEVDNEKTCVACLEEILQPCVLQCGHIFCHQC 260
            +++L  L E I  + ++ + C+ C + I Q  +  CGH++C  C
Sbjct: 1072 RRYLTNLYEHIVLKAESHQICIICRDIIKQGFITTCGHLYCSFC 1115


>SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 387

 Score = 29.9 bits (64), Expect = 0.31
 Identities = 10/30 (33%), Positives = 14/30 (46%)
 Frame = -3

Query: 349 VDNEKTCVACLEEILQPCVLQCGHIFCHQC 260
           +D+   C+ C E    P +  C H FC  C
Sbjct: 23  LDSSLRCLICHEYFRAPLITSCSHTFCSFC 52


>SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 269

 Score = 29.5 bits (63), Expect = 0.41
 Identities = 11/24 (45%), Positives = 13/24 (54%)
 Frame = -3

Query: 331 CVACLEEILQPCVLQCGHIFCHQC 260
           CV CL+         CGHIFC+ C
Sbjct: 206 CVICLDSPENLSCTPCGHIFCNFC 229


>SPAC6B12.07c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 456

 Score = 27.5 bits (58), Expect = 1.6
 Identities = 9/24 (37%), Positives = 11/24 (45%)
 Frame = -3

Query: 331 CVACLEEILQPCVLQCGHIFCHQC 260
           C  C     +P  L C H+FC  C
Sbjct: 360 CAICSNVAYKPVRLGCSHVFCLHC 383


>SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit
           1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 232

 Score = 26.6 bits (56), Expect = 2.9
 Identities = 19/62 (30%), Positives = 26/62 (41%), Gaps = 1/62 (1%)
 Frame = -3

Query: 412 LWQSVYXAKKHLDQLKEIPNEVD-NEKTCVACLEEILQPCVLQCGHIFCHQCC*AH*TVV 236
           +W     A   LD    + NE + N   C AC E ++   V  CG+     CC  H   V
Sbjct: 158 IWTFTNHALAELDAY--LHNEYESNLYECNACREIVIAGYVCDCGYCLHVYCC-KHLAHV 214

Query: 235 HC 230
           +C
Sbjct: 215 NC 216


>SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1133

 Score = 26.6 bits (56), Expect = 2.9
 Identities = 10/27 (37%), Positives = 12/27 (44%)
 Frame = -3

Query: 328 VACLEEILQPCVLQCGHIFCHQCC*AH 248
           + C E I  P +L C H  C  C   H
Sbjct: 879 ICCNEPIQNPLLLNCKHACCGDCLSEH 905


>SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 673

 Score = 26.6 bits (56), Expect = 2.9
 Identities = 10/25 (40%), Positives = 15/25 (60%), Gaps = 1/25 (4%)
 Frame = -3

Query: 331 CVACLEEI-LQPCVLQCGHIFCHQC 260
           C  CLEE  +   + +CGH++C  C
Sbjct: 214 CPFCLEEKPVAARMSRCGHVYCFSC 238


>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
           Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1958

 Score = 25.8 bits (54), Expect = 5.0
 Identities = 8/20 (40%), Positives = 15/20 (75%)
 Frame = +1

Query: 298 KVVIFPPSMQHMSFRYPLHW 357
           K++ +  ++Q +SF +PLHW
Sbjct: 694 KLIEYDIALQPVSFHHPLHW 713


>SPAC29B12.10c |||OPT oligopeptide transporter
           family|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 851

 Score = 25.8 bits (54), Expect = 5.0
 Identities = 14/43 (32%), Positives = 23/43 (53%)
 Frame = -1

Query: 360 FPMKWITKRHVLHAWRKYYNLVFFSVAIYSAINVARRTKQLCI 232
           F   ++ ++  +H WRK YN V  + A+ S + VA     LC+
Sbjct: 771 FIFNYVIRKRAIHWWRK-YNYV-LAAAMDSGVAVAGVVIFLCV 811


>SPBC30D10.05c |||sepiapterin reductase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 247

 Score = 25.0 bits (52), Expect = 8.8
 Identities = 12/35 (34%), Positives = 21/35 (60%), Gaps = 2/35 (5%)
 Frame = -1

Query: 327 LHAWRKYYNLVFFSV--AIYSAINVARRTKQLCIV 229
           ++ WRK +++ FFSV   +  AI   R+TK   ++
Sbjct: 100 INEWRKLFDINFFSVVETVKYAIPHLRKTKGTIVI 134


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,437,400
Number of Sequences: 5004
Number of extensions: 50293
Number of successful extensions: 138
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 134
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 138
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 271646730
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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