BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0901
(674 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC227.09 |||folylpolyglutamate synthase|Schizosaccharomyces po... 28 1.1
SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux transpo... 27 1.9
SPAC27E2.04c |mug155||sequence orphan|Schizosaccharomyces pombe|... 26 5.7
SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy... 26 5.7
SPBC16E9.05 |erg6||delta-sterol C-methyltransferase |Schizosacch... 25 7.6
SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy... 25 7.6
SPCC736.08 |cbf11||CBF1/Su|Schizosaccharomyces pombe|chr 3|||Manual 25 10.0
SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit ... 25 10.0
>SPAC227.09 |||folylpolyglutamate synthase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 417
Score = 28.3 bits (60), Expect = 1.1
Identities = 19/54 (35%), Positives = 24/54 (44%)
Frame = -1
Query: 344 IVLAMASVNRLTLGGASPG*ARARPPILVKWKGLRANKSPFFIKKTRWGVNTFT 183
+V + VN L S RA P L K K NK+ + I WG NTF+
Sbjct: 177 VVDGLNEVNVLNQLKLSAEETRAHPFYLAKGKS-GENKNEWIINTPNWGTNTFS 229
>SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux
transporter Bfr1|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1530
Score = 27.5 bits (58), Expect = 1.9
Identities = 21/63 (33%), Positives = 27/63 (42%), Gaps = 1/63 (1%)
Frame = -2
Query: 553 TNYGRLAKG-GSHLLQEIINYWCSVL*VRTIILFLPQSSPALRLQGLGAAAIL*YTVQVR 377
TN R A G ++ L I C R++ +P A L G+G AI YT
Sbjct: 642 TNLKRTAGGFWTYFLFLFIGATCMSAFFRSLAGIMPNVESASALGGIGVLAIAIYTGYAI 701
Query: 376 PNI 368
PNI
Sbjct: 702 PNI 704
>SPAC27E2.04c |mug155||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 187
Score = 25.8 bits (54), Expect = 5.7
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = -3
Query: 312 HTRWSLSWLSPCSPTYLGQMER 247
H +W+L L+PC PTY +R
Sbjct: 121 HQKWALR-LNPCLPTYFFHRQR 141
>SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 541
Score = 25.8 bits (54), Expect = 5.7
Identities = 11/21 (52%), Positives = 13/21 (61%)
Frame = +3
Query: 234 VGPEAFPFDQDRWASTGLARR 296
V PE F+ DRWA GLA +
Sbjct: 424 VYPEPETFNPDRWAPNGLAEQ 444
>SPBC16E9.05 |erg6||delta-sterol C-methyltransferase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 25.4 bits (53), Expect = 7.6
Identities = 15/50 (30%), Positives = 23/50 (46%)
Frame = -3
Query: 480 YKSVPSSYFCHKAVLH*GFKGWEQLLYSDILYK*DLISQEKRLESHCISY 331
YKSV +SY+ L+ GW Q + YK + +Q H ++Y
Sbjct: 71 YKSVVNSYYDLATDLY--EYGWSQSFHFSRFYKGEAFAQSIARHEHYLAY 118
>SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 4924
Score = 25.4 bits (53), Expect = 7.6
Identities = 13/32 (40%), Positives = 19/32 (59%)
Frame = -3
Query: 603 ISLLWDISKRT*SIGTVQITVGLLKEGATYYR 508
I L+W SK + G ++ +GLL E T+YR
Sbjct: 3799 IDLIWLASKVS-EAGIGKLDIGLLMEKPTFYR 3829
>SPCC736.08 |cbf11||CBF1/Su|Schizosaccharomyces pombe|chr 3|||Manual
Length = 613
Score = 25.0 bits (52), Expect = 10.0
Identities = 8/28 (28%), Positives = 18/28 (64%)
Frame = +2
Query: 350 SSLFS*DIRSHLYSISEYSSCSQPLKPQ 433
SS+F+ R+ + ++++ CSQP+ +
Sbjct: 212 SSIFNQSFRTEISIMNDFGQCSQPISEE 239
>SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit
Bgs2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1894
Score = 25.0 bits (52), Expect = 10.0
Identities = 13/39 (33%), Positives = 22/39 (56%), Gaps = 2/39 (5%)
Frame = +1
Query: 142 NIFLGH*SFL--LYRLVKVFTPHLVFFMKKGLLLARRPF 252
+I+LG + L L+ + V+ PHL++F L + PF
Sbjct: 1485 SIYLGSRTLLMLLFGTMTVWIPHLIYFWISTLAMCISPF 1523
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,755,696
Number of Sequences: 5004
Number of extensions: 57841
Number of successful extensions: 87
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 85
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 87
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 309878492
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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