Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0887
(677 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1815.01 |eno101|eno1|enolase|Schizosaccharomyces pombe|chr 2... 117 2e-27
SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase |Schizosa... 107 2e-24
SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 28 1.1
SPCC1672.03c |||guanine deaminase |Schizosaccharomyces pombe|chr... 27 1.9
SPCC1753.05 |rsm1||RNA export factor Rsm1|Schizosaccharomyces po... 26 4.4
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 25 7.6
SPBC4B4.10c |mug77|atg5|autophagy associated protein Atg5 |Schiz... 25 7.6
>SPBC1815.01 |eno101|eno1|enolase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 439
Score = 117 bits (281), Expect = 2e-27
Identities = 54/84 (64%), Positives = 69/84 (82%)
Frame = +1
Query: 7 EIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGVLT 186
++ ARQI+DSRGNPTVEVDL TE G+ RA VPSGASTG+ EALE+RD K+++ GKGVL
Sbjct: 5 KVFARQIYDSRGNPTVEVDLTTETGIHRAIVPSGASTGIWEALEMRDGDKTKWGGKGVLK 64
Query: 187 AIKNINELIAPELTKANLEVTQQE 258
A+ N+N +IAP + KANL+VT Q+
Sbjct: 65 AVGNVNNIIAPAVVKANLDVTDQK 88
Score = 111 bits (267), Expect = 9e-26
Identities = 58/110 (52%), Positives = 71/110 (64%), Gaps = 1/110 (0%)
Frame = +3
Query: 264 DELMLKLDGTENKSKLGANAIL*VSLXXXXXXXXXXNVPLYKHLADLAGNND-IVLPVPA 440
DE +LKLDGTENKSKLGANAIL VS+ +PL+K++A+ G VLPVP+
Sbjct: 91 DEFLLKLDGTENKSKLGANAILGVSMAICRAGAAQKKLPLWKYIAENFGTKGPYVLPVPS 150
Query: 441 FNVINGGSHAGNKLAMQEFMIFPQGHPPSVKP*GWVQKCTHHLKKIIKEK 590
FNV+NGGSHAG LA QEFMI P G P + W + H LK I K++
Sbjct: 151 FNVLNGGSHAGGDLAFQEFMILPTGAPSFSEAMRWGAETYHTLKSIAKKR 200
>SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 440
Score = 107 bits (256), Expect = 2e-24
Identities = 49/83 (59%), Positives = 65/83 (78%)
Frame = +1
Query: 7 EIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGVLT 186
+I +R I+DSRGNPTVEV+L TELG FR+ VPSGASTG EA ELRDN K+++ GKGV
Sbjct: 6 KIYSRSIYDSRGNPTVEVELTTELGTFRSMVPSGASTGEWEAKELRDNDKNKWGGKGVTI 65
Query: 187 AIKNINELIAPELTKANLEVTQQ 255
A+ N+N +I P L K+++++T Q
Sbjct: 66 AVHNVNNIIGPALVKSDIKITDQ 88
Score = 105 bits (251), Expect = 8e-24
Identities = 55/122 (45%), Positives = 73/122 (59%), Gaps = 1/122 (0%)
Frame = +3
Query: 228 KSQPRSNPTREIDELMLKLDGTENKSKLGANAIL*VSLXXXXXXXXXXNVPLYKHLADLA 407
KS + R IDE M+KLDGT +KSKLGAN+I+ VS+ +PLY+++ LA
Sbjct: 80 KSDIKITDQRGIDEFMIKLDGTNDKSKLGANSIVGVSMAVARAAAAFLKIPLYEYIGKLA 139
Query: 408 GNNDI-VLPVPAFNVINGGSHAGNKLAMQEFMIFPQGHPPSVKP*GWVQKCTHHLKKIIK 584
G+ +PVP+FNV+NGG HAG LA QEFMI P P + W + H LK + K
Sbjct: 140 GSKTTECIPVPSFNVLNGGRHAGGDLAFQEFMIMPIKAPTFSEGLRWGSEVYHTLKALAK 199
Query: 585 EK 590
+K
Sbjct: 200 KK 201
>SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 245
Score = 28.3 bits (60), Expect = 1.1
Identities = 22/72 (30%), Positives = 32/72 (44%)
Frame = +1
Query: 31 DSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGVLTAIKNINEL 210
D +G +VD ++A +ST LE R N + E V TAI + E
Sbjct: 140 DEKGPSLYQVDPSGTYFAWKATAIGKSSTAAKTFLEKRYNDELELDD-AVHTAILALKET 198
Query: 211 IAPELTKANLEV 246
ELT+ N+E+
Sbjct: 199 FEGELTEDNIEI 210
>SPCC1672.03c |||guanine deaminase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 527
Score = 27.5 bits (58), Expect = 1.9
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = +2
Query: 338 PSCC*GWCCQEE 373
PSCC G CC+EE
Sbjct: 490 PSCCGGHCCKEE 501
>SPCC1753.05 |rsm1||RNA export factor Rsm1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 296
Score = 26.2 bits (55), Expect = 4.4
Identities = 15/38 (39%), Positives = 23/38 (60%)
Frame = +1
Query: 196 NINELIAPELTKANLEVTQQERLMNSCLSWMALRTNPN 309
++NEL PE TK LEV+ +E ++CL W + P+
Sbjct: 106 SLNEL--PEKTKERLEVSLKEEHQDNCL-WRLHKFPPD 140
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 25.4 bits (53), Expect = 7.6
Identities = 14/54 (25%), Positives = 28/54 (51%), Gaps = 1/54 (1%)
Frame = -3
Query: 231 FCKFRSNEFIDIFDCGQNSLAMIFTLDV-IS*FKSFMNTSGCTRGYSCPEQAKL 73
F + +EF +IF+C ++LA++ +S + +N +G + C AK+
Sbjct: 657 FVEILESEFPNIFECLVDNLALLHIFQFWMSNEVTSVNCTGIVLSFCCDNLAKI 710
>SPBC4B4.10c |mug77|atg5|autophagy associated protein Atg5
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 261
Score = 25.4 bits (53), Expect = 7.6
Identities = 13/36 (36%), Positives = 18/36 (50%)
Frame = -2
Query: 520 GCPCGKIMNSCMASLFPACDPPLITLNAGTGRTMSL 413
G G+ +N + LFP+CD LI G T+ L
Sbjct: 201 GSSLGEFLNKRLPDLFPSCDKFLIVKPVIHGITIFL 236
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,877,032
Number of Sequences: 5004
Number of extensions: 59386
Number of successful extensions: 145
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 135
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 141
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 311890690
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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