Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0867
(578 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q9VVS4 Cluster: Mediator of RNA polymerase II transcrip... 88 1e-16
UniRef50_Q17NH9 Cluster: Mediator of RNA polymerase II transcrip... 83 3e-15
UniRef50_UPI0000E4A3BB Cluster: PREDICTED: similar to conserved ... 63 4e-09
UniRef50_Q9P086 Cluster: Mediator of RNA polymerase II transcrip... 52 1e-05
UniRef50_A7RKV3 Cluster: Predicted protein; n=1; Nematostella ve... 47 3e-04
UniRef50_UPI0001555AE6 Cluster: PREDICTED: hypothetical protein,... 44 0.003
UniRef50_Q8XAT1 Cluster: No significant matches; n=2; Enterobact... 35 1.2
UniRef50_UPI0000DB79C9 Cluster: PREDICTED: similar to kinectin 1... 33 3.7
UniRef50_Q8EWP9 Cluster: Predicted coiled-coil structure contain... 33 3.7
>UniRef50_Q9VVS4 Cluster: Mediator of RNA polymerase II
transcription subunit 11; n=4; Endopterygota|Rep:
Mediator of RNA polymerase II transcription subunit 11 -
Drosophila melanogaster (Fruit fly)
Length = 176
Score = 88.2 bits (209), Expect = 1e-16
Identities = 41/61 (67%), Positives = 46/61 (75%)
Frame = +3
Query: 216 KNTQSSGIKVE*TINYLTQVSTGQPHEGSGYASQKVLQMAWHRLEHVRSWVNELDRLKAS 395
K+ S K+ INYLTQVSTGQPHEGSGYAS KVLQMAWHR++H RS V EL+ KA
Sbjct: 50 KSLSSVESKLSEQINYLTQVSTGQPHEGSGYASAKVLQMAWHRIQHARSRVRELEETKAK 109
Query: 396 H 398
H
Sbjct: 110 H 110
Score = 66.9 bits (156), Expect = 3e-10
Identities = 33/60 (55%), Positives = 41/60 (68%)
Frame = +1
Query: 76 PMERIQVLDEIEKDIITXXXXXXXXXXELSKEKSGQKQAESNTSQFLRTLSQVESKLSEQ 255
P+++I LDEIEK+II EL KEKS QK AE+ + QFL++LS VESKLSEQ
Sbjct: 3 PLDKIHALDEIEKEIILCMQSAGQALQELGKEKSSQKNAETQSQQFLKSLSSVESKLSEQ 62
>UniRef50_Q17NH9 Cluster: Mediator of RNA polymerase II
transcription subunit 11; n=3; Endopterygota|Rep:
Mediator of RNA polymerase II transcription subunit 11 -
Aedes aegypti (Yellowfever mosquito)
Length = 132
Score = 83.4 bits (197), Expect = 3e-15
Identities = 39/62 (62%), Positives = 48/62 (77%), Gaps = 1/62 (1%)
Frame = +3
Query: 240 KVE*TINYLTQVSTGQPHEGSGYASQKVLQMAWHRLEHVRSWVNELDRLKASHL-ATPRT 416
K+ INYLTQVSTGQPHEGSGYA+ KVLQMAWHR++HV+S + EL+ K ++ AT R
Sbjct: 58 KLSEQINYLTQVSTGQPHEGSGYAAAKVLQMAWHRIQHVKSRIKELEECKIKYVQATNRL 117
Query: 417 AT 422
T
Sbjct: 118 QT 119
Score = 62.1 bits (144), Expect = 9e-09
Identities = 30/59 (50%), Positives = 43/59 (72%)
Frame = +1
Query: 79 MERIQVLDEIEKDIITXXXXXXXXXXELSKEKSGQKQAESNTSQFLRTLSQVESKLSEQ 255
+++IQVLD IEK+++ ELSKEK+ QK E++T+QFL++L+ VESKLSEQ
Sbjct: 4 IDKIQVLDSIEKELLLCLQSAGQALLELSKEKTSQKATETHTNQFLKSLNIVESKLSEQ 62
>UniRef50_UPI0000E4A3BB Cluster: PREDICTED: similar to conserved
hypothetical protein; n=2; Strongylocentrotus
purpuratus|Rep: PREDICTED: similar to conserved
hypothetical protein - Strongylocentrotus purpuratus
Length = 88
Score = 63.3 bits (147), Expect = 4e-09
Identities = 27/43 (62%), Positives = 35/43 (81%)
Frame = +3
Query: 255 INYLTQVSTGQPHEGSGYASQKVLQMAWHRLEHVRSWVNELDR 383
INYL+QVSTGQPHEGS Y++QK QMA HRLEH ++ + E+ +
Sbjct: 41 INYLSQVSTGQPHEGSSYSAQKEAQMAIHRLEHAKTKLTEIKK 83
Score = 37.9 bits (84), Expect = 0.17
Identities = 17/33 (51%), Positives = 23/33 (69%)
Frame = +1
Query: 157 ELSKEKSGQKQAESNTSQFLRTLSQVESKLSEQ 255
ELSK++ +KQ +SNT F +TL VE +L EQ
Sbjct: 8 ELSKDRPVEKQIDSNTKTFAKTLEAVEKRLMEQ 40
>UniRef50_Q9P086 Cluster: Mediator of RNA polymerase II
transcription subunit 11; n=15; Euteleostomi|Rep:
Mediator of RNA polymerase II transcription subunit 11 -
Homo sapiens (Human)
Length = 117
Score = 52.0 bits (119), Expect = 1e-05
Identities = 23/43 (53%), Positives = 33/43 (76%)
Frame = +3
Query: 255 INYLTQVSTGQPHEGSGYASQKVLQMAWHRLEHVRSWVNELDR 383
I YLTQV+TGQPHEGS Y+S+K QMA R+++ R ++++ R
Sbjct: 67 IRYLTQVATGQPHEGSSYSSRKDCQMALKRVDYARLKLSDVAR 109
Score = 37.9 bits (84), Expect = 0.17
Identities = 18/58 (31%), Positives = 33/58 (56%)
Frame = +1
Query: 82 ERIQVLDEIEKDIITXXXXXXXXXXELSKEKSGQKQAESNTSQFLRTLSQVESKLSEQ 255
ER++ L++IE++I ELSKEK+ ++ + + F ++ VE++LS Q
Sbjct: 9 ERLRALEDIEREIGAILQNAGTVILELSKEKTNERLLDRQAAAFTASVQHVEAELSAQ 66
>UniRef50_A7RKV3 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 116
Score = 47.2 bits (107), Expect = 3e-04
Identities = 21/44 (47%), Positives = 29/44 (65%)
Frame = +3
Query: 255 INYLTQVSTGQPHEGSGYASQKVLQMAWHRLEHVRSWVNELDRL 386
INYLTQV+TGQPHEGS Y K ++A R V+ + E+ ++
Sbjct: 64 INYLTQVATGQPHEGSTYGVDKDFELATSRTAIVKGQLQEVQKI 107
Score = 41.9 bits (94), Expect = 0.011
Identities = 21/63 (33%), Positives = 33/63 (52%)
Frame = +1
Query: 67 MAAPMERIQVLDEIEKDIITXXXXXXXXXXELSKEKSGQKQAESNTSQFLRTLSQVESKL 246
MA +R++ L+EIEKDI+ ELS E + ++F+++L VE L
Sbjct: 1 MAHSRDRLKQLEEIEKDIVKVMQSAGETIAELSNENPSEDMVNMKATEFVKSLEGVEKGL 60
Query: 247 SEQ 255
+EQ
Sbjct: 61 TEQ 63
>UniRef50_UPI0001555AE6 Cluster: PREDICTED: hypothetical protein,
partial; n=1; Ornithorhynchus anatinus|Rep: PREDICTED:
hypothetical protein, partial - Ornithorhynchus anatinus
Length = 150
Score = 44.0 bits (99), Expect = 0.003
Identities = 24/52 (46%), Positives = 33/52 (63%), Gaps = 1/52 (1%)
Frame = +3
Query: 273 VSTGQPHEGSGYASQKVLQMAWHRLEHVRSWVNELDRLKASHLAT-PRTATG 425
V+TGQPHEGS Y+++K QMA R+++ R V EL L T PR ++G
Sbjct: 1 VATGQPHEGSSYSARKDCQMALKRVDYARLKVGELACTCEQMLDTSPRLSSG 52
>UniRef50_Q8XAT1 Cluster: No significant matches; n=2;
Enterobacteriaceae|Rep: No significant matches -
Escherichia coli O157:H7
Length = 447
Score = 35.1 bits (77), Expect = 1.2
Identities = 21/69 (30%), Positives = 33/69 (47%), Gaps = 1/69 (1%)
Frame = +3
Query: 255 INYLTQVSTGQPHEGSGYASQKVLQMAWHRL-EHVRSWVNELDRLKASHLATPRTATGSV 431
+NY QV +PHE + + + + Q W L +HVR V L++ P TA +
Sbjct: 292 LNYAGQVKVNRPHEYAKFTLEHIRQ--WAALNKHVRKPVGYLEKWCKERNLAPTTARNYL 349
Query: 432 PNGNMTSSG 458
N +T+ G
Sbjct: 350 KNDGLTALG 358
>UniRef50_UPI0000DB79C9 Cluster: PREDICTED: similar to kinectin 1;
n=1; Apis mellifera|Rep: PREDICTED: similar to kinectin
1 - Apis mellifera
Length = 943
Score = 33.5 bits (73), Expect = 3.7
Identities = 22/58 (37%), Positives = 31/58 (53%)
Frame = +1
Query: 157 ELSKEKSGQKQAESNTSQFLRTLSQVESKLSEQ*IT*LKFQLDNHMKVLDMLHRRFCK 330
++S+E+ K+ E TSQ Q E K S + IT L+ QLD K LD + +F K
Sbjct: 502 KISQEELVNKEEELKTSQEQLNNVQTELKQSTENITQLEIQLDTVQKNLDTVKDKFDK 559
>UniRef50_Q8EWP9 Cluster: Predicted coiled-coil structure containing
protein; n=1; Mycoplasma penetrans|Rep: Predicted
coiled-coil structure containing protein - Mycoplasma
penetrans
Length = 828
Score = 33.5 bits (73), Expect = 3.7
Identities = 15/36 (41%), Positives = 23/36 (63%)
Frame = -3
Query: 348 LNDAKPFAKPSVKHIQNLHVVVQLKLESSNLLFTQL 241
LND + F+K S++ I NL + + E +NLLF Q+
Sbjct: 393 LNDGEYFSKKSIRKINNLCIQISEYTEKNNLLFEQV 428
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 534,675,475
Number of Sequences: 1657284
Number of extensions: 9621620
Number of successful extensions: 19731
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 19047
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 19706
length of database: 575,637,011
effective HSP length: 96
effective length of database: 416,537,747
effective search space used: 39987623712
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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