SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--0857
         (596 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1834.04 |hht1||histone H3 h3.1|Schizosaccharomyces pombe|chr...   144   1e-35
SPBC8D2.04 |hht2|h3.2|histone H3 h3.2|Schizosaccharomyces pombe|...   144   1e-35
SPBC1105.11c |hht3|h3.3|histone H3 h3.3|Schizosaccharomyces pomb...   144   1e-35
SPBC1105.17 |cnp1|sim2|CENP-A|Schizosaccharomyces pombe|chr 2|||...   105   4e-24
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa...    27   2.7  
SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schi...    27   2.7  
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc...    26   3.6  
SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subu...    26   4.8  
SPBC19G7.10c |||topoisomerase associated protein |Schizosaccharo...    26   4.8  
SPAC3G6.01 |hrp3||ATP-dependent DNA helicase Hrp3|Schizosaccharo...    25   6.3  
SPAC607.06c |||metallopeptidase|Schizosaccharomyces pombe|chr 1|...    25   6.3  

>SPAC1834.04 |hht1||histone H3 h3.1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 136

 Score =  144 bits (348), Expect = 1e-35
 Identities = 70/76 (92%), Positives = 72/76 (94%)
 Frame = +3

Query: 255 RRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCA 434
           RRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQS+AIGALQEA EAYLV LFEDTNLCA
Sbjct: 53  RRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAIGALQEAVEAYLVSLFEDTNLCA 112

Query: 435 IHAKRVTIMPKDIQLA 482
           IH KRVTI PKD+QLA
Sbjct: 113 IHGKRVTIQPKDMQLA 128



 Score =  105 bits (252), Expect = 5e-24
 Identities = 49/52 (94%), Positives = 52/52 (100%)
 Frame = +1

Query: 100 MARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGTVALREI 255
           MARTKQTARKSTGGKAPRKQLA+KAARK+AP+TGGVKKPHRYRPGTVALREI
Sbjct: 1   MARTKQTARKSTGGKAPRKQLASKAARKAAPATGGVKKPHRYRPGTVALREI 52


>SPBC8D2.04 |hht2|h3.2|histone H3 h3.2|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 136

 Score =  144 bits (348), Expect = 1e-35
 Identities = 70/76 (92%), Positives = 72/76 (94%)
 Frame = +3

Query: 255 RRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCA 434
           RRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQS+AIGALQEA EAYLV LFEDTNLCA
Sbjct: 53  RRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAIGALQEAVEAYLVSLFEDTNLCA 112

Query: 435 IHAKRVTIMPKDIQLA 482
           IH KRVTI PKD+QLA
Sbjct: 113 IHGKRVTIQPKDMQLA 128



 Score =  105 bits (252), Expect = 5e-24
 Identities = 49/52 (94%), Positives = 52/52 (100%)
 Frame = +1

Query: 100 MARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGTVALREI 255
           MARTKQTARKSTGGKAPRKQLA+KAARK+AP+TGGVKKPHRYRPGTVALREI
Sbjct: 1   MARTKQTARKSTGGKAPRKQLASKAARKAAPATGGVKKPHRYRPGTVALREI 52


>SPBC1105.11c |hht3|h3.3|histone H3 h3.3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 136

 Score =  144 bits (348), Expect = 1e-35
 Identities = 70/76 (92%), Positives = 72/76 (94%)
 Frame = +3

Query: 255 RRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCA 434
           RRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQS+AIGALQEA EAYLV LFEDTNLCA
Sbjct: 53  RRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAIGALQEAVEAYLVSLFEDTNLCA 112

Query: 435 IHAKRVTIMPKDIQLA 482
           IH KRVTI PKD+QLA
Sbjct: 113 IHGKRVTIQPKDMQLA 128



 Score =  105 bits (252), Expect = 5e-24
 Identities = 49/52 (94%), Positives = 52/52 (100%)
 Frame = +1

Query: 100 MARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGTVALREI 255
           MARTKQTARKSTGGKAPRKQLA+KAARK+AP+TGGVKKPHRYRPGTVALREI
Sbjct: 1   MARTKQTARKSTGGKAPRKQLASKAARKAAPATGGVKKPHRYRPGTVALREI 52


>SPBC1105.17 |cnp1|sim2|CENP-A|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 120

 Score =  105 bits (253), Expect = 4e-24
 Identities = 54/82 (65%), Positives = 68/82 (82%), Gaps = 6/82 (7%)
 Frame = +3

Query: 255 RRYQKSTELLIRKLPFQRLVREIAQDF----KTD--LRFQSAAIGALQEASEAYLVGLFE 416
           R+YQ+ST+LLI++LPF R+VREI+ +F     TD  LR+QS A+  LQEA+EA+LV LFE
Sbjct: 33  RKYQRSTDLLIQRLPFSRIVREISSEFVANFSTDVGLRWQSTALQCLQEAAEAFLVHLFE 92

Query: 417 DTNLCAIHAKRVTIMPKDIQLA 482
           DTNLCAIHAKRVTIM +D+QLA
Sbjct: 93  DTNLCAIHAKRVTIMQRDMQLA 114



 Score = 27.5 bits (58), Expect = 1.6
 Identities = 16/31 (51%), Positives = 20/31 (64%), Gaps = 4/31 (12%)
 Frame = +1

Query: 175 ARKSAPSTGG--VKKPH--RYRPGTVALREI 255
           A+KS  +  G  + +P   RYRPGT ALREI
Sbjct: 2   AKKSLMAEPGDPIPRPRKKRYRPGTTALREI 32


>SPCC584.04 |sup35|erf3|translation release factor eRF3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 662

 Score = 26.6 bits (56), Expect = 2.7
 Identities = 22/77 (28%), Positives = 38/77 (49%), Gaps = 2/77 (2%)
 Frame = +3

Query: 216 TSLSPWYSGPS*NRRYQKSTELLIRKL--PFQRLVREIAQDFKTDLRFQSAAIGALQEAS 389
           +S+ PWY GPS    Y  S   L RK+  PF   +    +D  T L  +  A G++++ S
Sbjct: 440 SSVCPWYQGPS-LLEYLDSMTHLERKVNAPFIMPIASKYKDLGTILEGKIEA-GSIKKNS 497

Query: 390 EAYLVGLFEDTNLCAIH 440
              ++ + +   + AI+
Sbjct: 498 NVLVMPINQTLEVTAIY 514


>SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein
           homolog|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 3071

 Score = 26.6 bits (56), Expect = 2.7
 Identities = 19/67 (28%), Positives = 29/67 (43%), Gaps = 1/67 (1%)
 Frame = -1

Query: 467 LRHDGHTFGVNSTQVGVFEQANEISLACLLESSDGGRLETQI-SFEILSDFSHKTLERQL 291
           LR+   T  +    + +  QA        LE S     + Q+ SFEIL     +TLE   
Sbjct: 73  LRNKSLTINIEDIYLSIHPQAKNSLTRDELEQSQQALKQEQLDSFEILRKNFRETLEESS 132

Query: 290 TDKQLSR 270
           ++  +SR
Sbjct: 133 SNPNISR 139


>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
           Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 227

 Score = 26.2 bits (55), Expect = 3.6
 Identities = 10/25 (40%), Positives = 16/25 (64%)
 Frame = +2

Query: 95  KQWLVLNKQPVNLLEVKLRVNNLLQ 169
           K++L L K PV L++ K    N+L+
Sbjct: 12  KEYLALQKNPVELVDAKPATENILE 36


>SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1405

 Score = 25.8 bits (54), Expect = 4.8
 Identities = 18/62 (29%), Positives = 29/62 (46%)
 Frame = -1

Query: 428 QVGVFEQANEISLACLLESSDGGRLETQISFEILSDFSHKTLERQLTDKQLSRLLITTIS 249
           Q+G F +AN I  A  + +      E +   EIL        ER L+ K+ +R   T+ +
Sbjct: 112 QIGAFFKANAIQPASRIANRKNNEKEDEFMAEILGSIDQDIPER-LSTKKGNRSHTTSNA 170

Query: 248 RR 243
           +R
Sbjct: 171 KR 172


>SPBC19G7.10c |||topoisomerase associated protein
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 744

 Score = 25.8 bits (54), Expect = 4.8
 Identities = 19/59 (32%), Positives = 28/59 (47%), Gaps = 2/59 (3%)
 Frame = +3

Query: 207 QEATSLSPWYSGPS*NRRYQKSTELLIRKL--PFQRLVREIAQDFKTDLRFQSAAIGAL 377
           +EA+     Y GPS NRR  + +E  ++KL    QRLV    +  K        A+G +
Sbjct: 359 EEASHFIQTYLGPSNNRRRGRRSENPMQKLQQQLQRLVSSAKERPKATQLSLEGALGKI 417


>SPAC3G6.01 |hrp3||ATP-dependent DNA helicase
           Hrp3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1388

 Score = 25.4 bits (53), Expect = 6.3
 Identities = 19/58 (32%), Positives = 28/58 (48%), Gaps = 5/58 (8%)
 Frame = +3

Query: 258 RYQKSTELLIRKLPFQRLVREIAQ--DFKTDLRF---QSAAIGALQEASEAYLVGLFE 416
           +++ S  LLI   P Q  +RE+A   DF    +F   +   + A  E  EAY+  L E
Sbjct: 526 QFKNSNRLLITGTPLQNNIRELAALVDFLMPGKFEIREEINLEAPDEEQEAYIRSLQE 583


>SPAC607.06c |||metallopeptidase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 612

 Score = 25.4 bits (53), Expect = 6.3
 Identities = 9/21 (42%), Positives = 15/21 (71%)
 Frame = -1

Query: 383 LLESSDGGRLETQISFEILSD 321
           L  + DGG+LE Q+ +++L D
Sbjct: 72  LFTTDDGGKLELQVYYQLLVD 92


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,134,482
Number of Sequences: 5004
Number of extensions: 40064
Number of successful extensions: 116
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 109
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 115
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 260219058
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -