BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0854
(595 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC417.08 |tef3||translation elongation factor eEF3|Schizosacch... 28 0.89
SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces pomb... 28 1.2
SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2 |Schizosacch... 27 2.7
SPCC777.04 |||amino acid transporter |Schizosaccharomyces pombe|... 26 3.6
SPBC1861.02 |abp2||ARS binding protein Abp2|Schizosaccharomyces ... 26 4.8
SPAP27G11.15 |slx1||structure-specific endonuclease catalytic su... 25 6.3
SPBC11G11.05 |rpa34||DNA-directed RNA polymerase I complex subun... 25 6.3
SPBC3E7.06c |||membrane transporter|Schizosaccharomyces pombe|ch... 25 8.3
>SPCC417.08 |tef3||translation elongation factor
eEF3|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1047
Score = 28.3 bits (60), Expect = 0.89
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = +3
Query: 117 VNCSNMEQKFPSPLFVFGEKVRTRAI 194
+ S ME KFP P F+ G K + RAI
Sbjct: 647 LGASEMEFKFPEPGFLEGVKTKQRAI 672
>SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 398
Score = 27.9 bits (59), Expect = 1.2
Identities = 17/44 (38%), Positives = 22/44 (50%)
Frame = -2
Query: 525 GTFRDSXDGHVDGLRFYVCSHRASVTQSSRRHSTTFRSAGENCS 394
G R S G +D YVC SV ++ + T R AGE+CS
Sbjct: 305 GHGRCSKYGQLDSC--YVCQCSNSVVSNAAGQNKTIRWAGESCS 346
>SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2176
Score = 26.6 bits (56), Expect = 2.7
Identities = 10/23 (43%), Positives = 13/23 (56%)
Frame = +2
Query: 482 RRPSTCPSXESRKVPKNRFPWXK 550
R+ CPS R+V K FPW +
Sbjct: 1168 RQFPNCPSEVIRRVEKKEFPWQR 1190
>SPCC777.04 |||amino acid transporter |Schizosaccharomyces pombe|chr
3|||Manual
Length = 521
Score = 26.2 bits (55), Expect = 3.6
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = -2
Query: 477 YVCSHRASVTQSSRRHSTTFRSAGE 403
Y+C HRA++ Q FRS G+
Sbjct: 430 YICYHRATIVQQIPTERIPFRSWGQ 454
>SPBC1861.02 |abp2||ARS binding protein Abp2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 527
Score = 25.8 bits (54), Expect = 4.8
Identities = 32/124 (25%), Positives = 61/124 (49%), Gaps = 3/124 (2%)
Frame = +2
Query: 47 LSTPREEEGIND--GRETG-IDTQDLSELQQHGAEVPLASVRFRREGAHPGHQLQADHHG 217
+S+P+E +G+ND R+T ++T+++ ++ A VP R+ G PG + +
Sbjct: 296 VSSPKESDGLNDSWSRQTNQVETENV----ENEAGVP------RKRGRPPGARNKIKRL- 344
Query: 218 RSHTSAALRL*QMEWVDNVVRLDNWLNFQMSPRSAALIIALRPAIERIVERAAAQPDAAL 397
RS S +L L + W + +L + N + RSA +A P +E + + + +
Sbjct: 345 RSEPSVSLTL-SISWYERFEKLMHAQNTML--RSAFSHVARLP-METVSQLLSHYTETIS 400
Query: 398 QFSP 409
Q+ P
Sbjct: 401 QYLP 404
>SPAP27G11.15 |slx1||structure-specific endonuclease catalytic
subunit |Schizosaccharomyces pombe|chr 1|||Manual
Length = 271
Score = 25.4 bits (53), Expect = 6.3
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = -1
Query: 112 VLCINAGFPSVVNTFLFPWCRQTLG 38
+ C+ GFP+ V+ F W Q LG
Sbjct: 57 ISCLVYGFPNKVSALKFEWNWQNLG 81
>SPBC11G11.05 |rpa34||DNA-directed RNA polymerase I complex subunit
Rpa34 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 251
Score = 25.4 bits (53), Expect = 6.3
Identities = 14/39 (35%), Positives = 20/39 (51%), Gaps = 1/39 (2%)
Frame = +1
Query: 454 GRPV-TTNIKTKTVNMPIXRIPKGSKKPIPMEXGIGGKP 567
G+PV TTN K N+ + R+P G + +G KP
Sbjct: 60 GKPVLTTNDLKKESNVFLVRLPPGMSADQISQLKVGSKP 98
>SPBC3E7.06c |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 577
Score = 25.0 bits (52), Expect = 8.3
Identities = 10/16 (62%), Positives = 12/16 (75%)
Frame = -1
Query: 193 MARVRTFSPKTNRGEG 146
M+ RT SP TNRG+G
Sbjct: 1 MSNPRTKSPNTNRGQG 16
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,339,360
Number of Sequences: 5004
Number of extensions: 43536
Number of successful extensions: 123
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 121
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 123
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 258201856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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