BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--0838 (573 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAP27G11.06c |||AP-1 adaptor complex subunit |Schizosaccharomyc... 137 1e-33 SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosacc... 101 6e-23 SPAC30D11.05 |aps3||AP-3 adaptor complex subunit Aps3 |Schizosac... 73 4e-14 SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosac... 32 0.052 SPBC15C4.01c |oca3||TPR repeat protein Oca3|Schizosaccharomyces ... 26 4.5 SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosacc... 25 6.0 SPCC550.11 |||karyopherin|Schizosaccharomyces pombe|chr 3|||Manual 25 6.0 >SPAP27G11.06c |||AP-1 adaptor complex subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 162 Score = 137 bits (331), Expect = 1e-33 Identities = 56/92 (60%), Positives = 78/92 (84%) Frame = +1 Query: 259 RELITTVLARKPKMCSFLEWKDVKVVYKRYASLYFCCAMEQEDNELLTLELIHRYVELLD 438 R++ + V+ RKPKMC+F+E+K K+VY+RYASL+F C +EQ+DNEL+ LE+IH++VE LD Sbjct: 34 RDVSSLVITRKPKMCNFVEYKGEKIVYRRYASLFFVCGIEQDDNELIILEVIHKFVECLD 93 Query: 439 KYFGXVCELDIIFNFEKAYFILDDWFLGGXLQ 534 KYFG VCELD+IFNFEKAY+++++ L G LQ Sbjct: 94 KYFGNVCELDLIFNFEKAYYVMEELLLAGELQ 125 Score = 31.5 bits (68), Expect = 0.091 Identities = 12/19 (63%), Positives = 16/19 (84%) Frame = +2 Query: 167 MQFMLLFSRQGKLRLQKWY 223 ++F LL SRQGK+RL KW+ Sbjct: 3 IKFFLLVSRQGKVRLAKWF 21 >SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 143 Score = 101 bits (243), Expect = 6e-23 Identities = 43/76 (56%), Positives = 58/76 (76%) Frame = +1 Query: 304 SFLEWKDVKVVYKRYASLYFCCAMEQEDNELLTLELIHRYVELLDKYFGXVCELDIIFNF 483 +FLEW++ K+VY+RYA LYFC ++ DN+L LE+IH +VE+LD +FG VCELD+IFNF Sbjct: 49 NFLEWENSKLVYRRYAGLYFCFCVDSTDNDLAILEMIHFFVEILDSFFGNVCELDLIFNF 108 Query: 484 EKAYFILDDWFLGGXL 531 K ILD+ LGG + Sbjct: 109 YKVSAILDEIILGGEI 124 Score = 30.3 bits (65), Expect = 0.21 Identities = 13/25 (52%), Positives = 18/25 (72%) Frame = +2 Query: 164 MMQFMLLFSRQGKLRLQKWYVAHPD 238 M+QF+L+ +R GK RL K+YV D Sbjct: 1 MIQFILIQNRHGKNRLSKYYVPFDD 25 >SPAC30D11.05 |aps3||AP-3 adaptor complex subunit Aps3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 165 Score = 72.5 bits (170), Expect = 4e-14 Identities = 34/92 (36%), Positives = 58/92 (63%), Gaps = 4/92 (4%) Frame = +1 Query: 262 ELITTVLARKPKMCSFLEWKDV----KVVYKRYASLYFCCAMEQEDNELLTLELIHRYVE 429 ++ V R P C+FLE + +++Y++YA+LYF +++ ++EL L+LI +VE Sbjct: 34 DIYAAVSTRPPTACNFLESNLIAGKNRIIYRQYATLYFVFVVDEGESELGILDLIQVFVE 93 Query: 430 LLDKYFGXVCELDIIFNFEKAYFILDDWFLGG 525 LD+ F VCELD++F F++ + IL + GG Sbjct: 94 ALDRCFNNVCELDLVFKFQEIHAILAEVVSGG 125 >SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 446 Score = 32.3 bits (70), Expect = 0.052 Identities = 16/67 (23%), Positives = 31/67 (46%) Frame = +1 Query: 334 VYKRYASLYFCCAMEQEDNELLTLELIHRYVELLDKYFGXVCELDIIFNFEKAYFILDDW 513 +Y ++ LY + N ++ LE + ++ L YFG + E + N + +LD+ Sbjct: 55 IYTKHEDLYVVAITKGNPNVMIVLEFLESLIQDLTHYFGKLNENTVKDNVSFIFELLDEM 114 Query: 514 FLGGXLQ 534 G +Q Sbjct: 115 IDYGIIQ 121 >SPBC15C4.01c |oca3||TPR repeat protein Oca3|Schizosaccharomyces pombe|chr 2|||Manual Length = 282 Score = 25.8 bits (54), Expect = 4.5 Identities = 12/38 (31%), Positives = 19/38 (50%) Frame = +1 Query: 334 VYKRYASLYFCCAMEQEDNELLTLELIHRYVELLDKYF 447 ++ R LYF A N +L+ R VE+ ++YF Sbjct: 175 LFARLGDLYFVLAQSNATNYWFSLKHYCRSVEICEEYF 212 >SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 426 Score = 25.4 bits (53), Expect = 6.0 Identities = 16/80 (20%), Positives = 31/80 (38%) Frame = +1 Query: 310 LEWKDVKVVYKRYASLYFCCAMEQEDNELLTLELIHRYVELLDKYFGXVCELDIIFNFEK 489 L + + +Y + +Y + + + L + + ++ YF + E I NF Sbjct: 50 LTHEGINYIYIHHNDVYLLALSKMNSDAMEMLVFLRKMADVFIDYFKELQEESIRDNFVL 109 Query: 490 AYFILDDWFLGGXLQVNPVK 549 Y +LD+ G Q K Sbjct: 110 VYELLDEIMDFGFPQTTETK 129 >SPCC550.11 |||karyopherin|Schizosaccharomyces pombe|chr 3|||Manual Length = 1029 Score = 25.4 bits (53), Expect = 6.0 Identities = 11/27 (40%), Positives = 17/27 (62%), Gaps = 3/27 (11%) Frame = +1 Query: 373 MEQEDNELLTL---ELIHRYVELLDKY 444 M +ED EL L E IH+Y+++ D + Sbjct: 361 MSEEDEELWELDPVEFIHKYIDIYDDF 387 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,254,854 Number of Sequences: 5004 Number of extensions: 43452 Number of successful extensions: 122 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 115 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 122 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 244081442 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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